BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,309,476	C→T	A27T (GCC→ACC)	yojL ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,309,476	0	C	T	97.7%	451.1 / ‑3.2	133	A27T (GCC→ACC)	yojL	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (67/63); total (68/65)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 137 total aligned reads displayed.

GTTTGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCT  >  NC_000913/2309386‑2309567

gTTTGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTtggtgg                                                                                           <  2:2505920/93‑1 (MQ=255)

 tttGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTggg                                                                                          <  2:1617966/93‑1 (MQ=255)

    ggCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTtg                                                                                       >  1:3957718/1‑93 (MQ=255)

     gCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTTCCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGtttt                                                                                      <  2:4744814/93‑3 (MQ=255)

     gCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTtgt                                                                                      <  1:1557589/93‑1 (MQ=255)

     gCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTtgt                                                                                      >  1:4106128/1‑93 (MQ=255)

      cGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTtgtg                                                                                     <  2:2111411/93‑1 (MQ=255)

        tCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGggtggtggt                                                                                   >  2:1610777/1‑93 (MQ=255)

        tCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGt                                                                                   <  1:2787498/93‑1 (MQ=255)

         cAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGtt                                                                                  <  1:1103297/93‑1 (MQ=255)

            tGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGttttt                                                                               <  1:102507/93‑1 (MQ=255)

             gCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTg                                                                              <  1:1909717/93‑1 (MQ=255)

             gCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTg                                                                              <  1:4301417/93‑1 (MQ=255)

              cccGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGa                                                                             <  1:877568/93‑1 (MQ=255)

               ccGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGAt                                                                            <  1:2218852/93‑1 (MQ=255)

               ccGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGAt                                                                            <  1:4181978/93‑1 (MQ=255)

                cGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATc                                                                           >  2:885688/1‑93 (MQ=255)

                 gggATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCg                                                                          >  1:2079943/1‑93 (MQ=255)

                 gggATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCg                                                                          >  1:326199/1‑93 (MQ=255)

                  ggATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGc                                                                         >  1:3793023/1‑93 (MQ=255)

                   gATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGca                                                                        >  2:3322034/1‑93 (MQ=255)

                    aTGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaa                                                                       <  2:3751444/93‑1 (MQ=255)

                    aTGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaa                                                                       >  1:4058669/1‑93 (MQ=255)

                     tGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaac                                                                      >  2:2106517/1‑93 (MQ=255)

                       cTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaacca                                                                    <  2:3007373/93‑1 (MQ=255)

                        tGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcaaccaa                                                                   <  2:3116041/93‑1 (MQ=255)

                         ggCACTCCAGAAAGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGcacccaac                                                                  <  2:1504107/93‑1 (MQ=255)

                         ggCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGATTTGGTGGGCTGGGGTTTTTGATCGcaaacaac                                                                  >  2:1927486/1‑93 (MQ=255)

                            aCGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACaaa                                                               <  1:1370700/93‑1 (MQ=255)

                              gCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaaga                                                             >  2:4404027/1‑93 (MQ=255)

                              gCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaaga                                                             <  1:3097007/93‑1 (MQ=255)

                               ccAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaa                                                            <  1:4225937/93‑1 (MQ=255)

                               ccAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaa                                                            <  2:1199179/93‑1 (MQ=255)

                                cAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaag                                                           <  1:2111998/93‑1 (MQ=255)

                                cAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaag                                                           <  2:3443202/93‑1 (MQ=255)

                                cAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaag                                                           <  2:4723727/93‑1 (MQ=255)

                                cAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaag                                                           <  2:2890420/93‑1 (MQ=255)

                                cAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaag                                                           >  2:367975/1‑93 (MQ=255)

                                cAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaag                                                           <  1:2401508/93‑1 (MQ=255)

                                cAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAaagaag                                                           >  1:3106751/1‑93 (MQ=255)

                                 aGAAGGTTCCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAagaaga                                                          <  2:721065/93‑1 (MQ=255)

                                 aGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAagaaga                                                          <  2:1156441/93‑1 (MQ=255)

                                   aaGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgc                                                        <  1:3899119/93‑1 (MQ=255)

                                    aGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgcg                                                       >  1:4683516/1‑93 (MQ=255)

                                    aGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgcg                                                       >  1:4683517/1‑93 (MQ=255)

                                     ggTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAgcgc                                                      >  2:3940011/1‑93 (MQ=255)

                                         cccATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGca                                                  >  2:3323070/1‑93 (MQ=255)

                                         cccATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAACAGCGCGGca                                                  >  2:3330971/1‑93 (MQ=255)

                                          ccATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGcag                                                 >  1:2505949/1‑93 (MQ=255)

                                             tAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGcagcca                                              <  1:2214596/93‑1 (MQ=255)

                                               gTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGcagccagc                                            >  1:3901496/1‑93 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagca                                           >  1:59479/1‑93 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagca                                           >  2:507634/1‑93 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagca                                           >  1:1229576/1‑93 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagca                                           >  1:374458/1‑93 (MQ=255)

                                                ttttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAAACAACAAACAAGAGCCCGGAAGCcagca                                           >  1:374321/1‑93 (MQ=255)

                                                 tttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagcag                                          <  2:148170/93‑1 (MQ=255)

                                                 tttGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCcagcag                                          <  2:78950/93‑1 (MQ=255)

                                                    gCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGc                                       >  2:2827987/1‑93 (MQ=255)

                                                     ccTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGcc                                      >  1:3058126/1‑93 (MQ=255)

                                                      cTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCa                                     <  2:1050722/93‑1 (MQ=255)

                                                      cTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCa                                     >  1:3051160/1‑93 (MQ=255)

                                                      cTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCa                                     >  1:2242112/1‑93 (MQ=255)

                                                       ttCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAc                                    >  1:147756/1‑93 (MQ=255)

                                                       ttCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAc                                    >  2:2158408/1‑93 (MQ=255)

                                                        tCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcg                                   <  1:764814/93‑1 (MQ=255)

                                                        tCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcg                                   >  1:2873102/1‑93 (MQ=255)

                                                        tCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcg                                   >  2:4338818/1‑93 (MQ=255)

                                                          aaGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCCcgcg                                 <  2:3646655/93‑1 (MQ=255)

                                                          aaGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcgcg                                 <  1:1774515/93‑1 (MQ=255)

                                                          aaGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcgcg                                 <  1:2328635/93‑1 (MQ=255)

                                                          aaGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcgcg                                 <  1:923699/93‑1 (MQ=255)

                                                          aaGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCAcgcg                                 >  2:1965443/1‑93 (MQ=255)

                                                           aGAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCTGTGCCACGCgg                                >  2:2327160/1‑93 (MQ=255)

                                                            gAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCggg                               <  1:588539/93‑1 (MQ=255)

                                                            gAACGGTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCggg                               <  2:2499352/93‑1 (MQ=255)

                                                                ggTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTaaa                           <  2:4132547/93‑1 (MQ=255)

                                                                 gTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCCGTGCCGCGCGGGTacac                          >  1:1653316/1‑90 (MQ=255)

                                                                 gTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAg                          >  2:3539183/1‑93 (MQ=255)

                                                                 gTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAg                          >  1:2204911/1‑93 (MQ=255)

                                                                 gTAACTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAg                          >  2:2571164/1‑93 (MQ=255)

                                                                    aCTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCtt                       <  2:2384346/93‑1 (MQ=255)

                                                                    aCTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCtt                       >  1:3507023/1‑93 (MQ=255)

                                                                    aCTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCtt                       >  1:2854466/1‑93 (MQ=255)

                                                                     cTTCAGTAGCGTGTGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTa                      <  2:556084/93‑1 (MQ=255)

                                                                     cTTCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTa                      <  1:271519/93‑1 (MQ=255)

                                                                       tCAGTAGCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTAtt                    <  2:3225229/93‑1 (MQ=255)

                                                                            ggCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCAt               >  1:3240701/2‑93 (MQ=255)

                                                                             gCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATa              <  2:3026813/93‑1 (MQ=255)

                                                                             gCGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATa              >  2:1705373/1‑93 (MQ=255)

                                                                             gCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATa              >  2:741505/1‑93 (MQ=255)

                                                                              cGTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATAc             <  1:3185319/93‑1 (MQ=255)

                                                                              cGTGGGGTTTGGTGGGTTGTGGTTTTTGACCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATAc             <  1:3185318/93‑1 (MQ=255)

                                                                               gTGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACg            >  1:4298260/1‑93 (MQ=255)

                                                                                tGGGTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGt           >  2:3088583/1‑93 (MQ=255)

                                                                                  ggTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGa         <  2:2786394/93‑1 (MQ=255)

                                                                                   gTTTTGGTGGTTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGAt        <  2:2030095/93‑1 (MQ=255)

                                                                                   gTTTTGGTGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGAt        >  1:59908/1‑93 (MQ=255)

                                                                                      ttGGTGGGTTGTGGTTTTTGATCGCACCCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTAt     <  1:4400942/93‑1 (MQ=255)

                                                                                        ggCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATcc   <  2:4111252/93‑1 (MQ=255)

GTTTGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCT  >  NC_000913/2309386‑2309567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`