BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,327,687	A→G	V44A (GTG→GCG)	b2226 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,327,687	0	A	G	97.8%	419.7 / ‑1.0	137	V44A (GTG→GCG)	b2226	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (76/58); total (77/60)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

Only 100 of 143 total aligned reads displayed.

TGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAG  >  NC_000913/2327597‑2327775

tGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTcgcg                                                                                        >  1:2195518/1‑93 (MQ=255)

    tATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtg                                                                                    >  1:4465819/1‑93 (MQ=255)

    tATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtg                                                                                    <  2:1310690/93‑1 (MQ=255)

     aTCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtga                                                                                   >  1:4623630/1‑93 (MQ=255)

     aTCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtga                                                                                   <  2:3814042/93‑1 (MQ=255)

      tcctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgat                                                                                  <  1:977359/93‑1 (MQ=255)

       cctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgatg                                                                                 <  1:2716616/93‑1 (MQ=255)

        ctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgatga                                                                                <  2:4291120/93‑1 (MQ=255)

        ctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgatga                                                                                >  2:4053607/1‑93 (MQ=255)

         tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                               >  1:966222/1‑93 (MQ=255)

         tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                               >  1:3416169/1‑93 (MQ=255)

         cccagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                               <  1:2763124/92‑1 (MQ=255)

          ccagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacg                                                                              >  1:3183457/1‑93 (MQ=255)

           cagccagTCGTAAACATACAGTTTCCACAAACTCCCCAACGCCGTGTTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacga                                                                             <  2:4284378/93‑1 (MQ=255)

           cagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacga                                                                             >  1:2576787/1‑93 (MQ=255)

           cagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacga                                                                             >  1:1748468/1‑93 (MQ=255)

              ccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAg                                                                          >  2:2229913/1‑93 (MQ=255)

               cagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGc                                                                         <  2:4084967/93‑1 (MQ=255)

                agGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCt                                                                        >  1:3163707/1‑93 (MQ=255)

                agGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCt                                                                        >  2:1599610/1‑93 (MQ=255)

                agGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCt                                                                        >  1:158904/1‑93 (MQ=255)

                 ggCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTg                                                                       <  2:3780224/93‑1 (MQ=255)

                 ggCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTg                                                                       >  2:4608142/1‑93 (MQ=255)

                 ggCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTg                                                                       >  1:552144/1‑93 (MQ=255)

                    gTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATa                                                                    >  1:1091777/1‑93 (MQ=255)

                     tAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATAc                                                                   >  2:4581575/1‑93 (MQ=255)

                     tAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATAc                                                                   >  2:551194/1‑93 (MQ=255)

                      aaaCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACa                                                                  <  2:3099322/93‑1 (MQ=255)

                       aaCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAg                                                                 >  2:1311973/1‑93 (MQ=255)

                        aCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGt                                                                <  1:4208978/93‑1 (MQ=255)

                        aCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGt                                                                <  2:2166171/93‑1 (MQ=255)

                        aCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGt                                                                <  2:742952/93‑1 (MQ=255)

                         cATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGtt                                                               <  2:83534/93‑1 (MQ=255)

                         cATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGtt                                                               <  2:3247840/93‑1 (MQ=255)

                          aTACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTg                                                              >  2:312801/1‑93 (MQ=255)

                          aTACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTg                                                              <  1:2990035/93‑1 (MQ=255)

                           tACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGa                                                             >  2:1278201/1‑93 (MQ=255)

                           tACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGa                                                             >  2:4149288/1‑93 (MQ=255)

                           tACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGa                                                             <  1:4517073/93‑1 (MQ=255)

                             cAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATc                                                           <  1:4005460/93‑1 (MQ=255)

                             cAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATc                                                           >  1:142560/1‑93 (MQ=255)

                               gTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGt                                                         >  2:2425747/1‑93 (MQ=255)

                                tttCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtg                                                        >  2:2585203/1‑93 (MQ=255)

                                  tCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgct                                                      <  2:192945/93‑1 (MQ=255)

                                    cacaAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgctgc                                                    >  2:3074285/1‑93 (MQ=255)

                                    cacaAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgctgc                                                    <  2:233950/93‑1 (MQ=255)

                                    cacaAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgctgc                                                    >  1:4488776/1‑93 (MQ=255)

                                      caAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTgcgc                                                  <  1:2871372/93‑1 (MQ=255)

                                      caAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTgcgc                                                  >  1:3676993/1‑93 (MQ=255)

                                       aaaCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCa                                                 <  1:4132923/93‑1 (MQ=255)

                                       aaaCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCa                                                 <  1:469490/93‑1 (MQ=255)

                                        aaCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAc                                                >  2:4051922/1‑93 (MQ=255)

                                             ccTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCgcgc                                           <  1:1426889/93‑1 (MQ=255)

                                               tAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgcag                                         >  2:4326322/1‑93 (MQ=255)

                                                 aCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCATCGCgcacca                                       >  2:2045159/1‑93 (MQ=255)

                                                 aCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgcagca                                       >  2:4388392/1‑93 (MQ=255)

                                                 aCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgcagca                                       >  1:1877856/1‑93 (MQ=255)

                                                  cGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCAc                                      >  2:3300918/1‑93 (MQ=255)

                                                   ggCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCAcc                                     <  2:2866711/93‑1 (MQ=255)

                                                    gCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCa                                    >  2:3608837/1‑93 (MQ=255)

                                                    gCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCa                                    >  2:4720078/1‑93 (MQ=255)

                                                     cGTGTTGAGCTAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAg                                   <  2:1496676/93‑1 (MQ=255)

                                                     cGTGATGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCTCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAg                                   <  1:4428532/93‑1 (MQ=255)

                                                      gtggtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGt                                  >  1:2198281/1‑93 (MQ=255)

                                                      gtggtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGt                                  >  1:3596379/1‑93 (MQ=255)

                                                      gtggtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGt                                  >  1:3767678/1‑93 (MQ=255)

                                                       tggtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGtt                                 >  2:629710/1‑93 (MQ=255)

                                                        ggtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTg                                <  1:3830858/93‑1 (MQ=255)

                                                           gAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTaa                             <  2:3194962/93‑1 (MQ=255)

                                                           gAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTaa                             >  1:816147/1‑93 (MQ=255)

                                                             gCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACg                           >  1:3288408/1‑93 (MQ=255)

                                                             gCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACg                           <  1:738265/93‑1 (MQ=255)

                                                             gCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACg                           <  2:3317548/93‑1 (MQ=255)

                                                              cGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACgg                          <  2:1017966/93‑1 (MQ=255)

                                                              cGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACgg                          <  1:169468/93‑1 (MQ=255)

                                                              cGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACgc                          >  1:665604/1‑92 (MQ=255)

                                                                  tCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtc                      <  2:2011950/93‑1 (MQ=255)

                                                                     ggTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcc                   >  2:4634396/1‑93 (MQ=255)

                                                                      gTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcct                  <  1:1845199/93‑1 (MQ=255)

                                                                      gTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcct                  <  2:1207813/93‑1 (MQ=255)

                                                                       tAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcctc                 <  2:3546022/93‑1 (MQ=255)

                                                                       tAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcctc                 >  1:1382100/1‑93 (MQ=255)

                                                                       tAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcctc                 >  1:790012/1‑93 (MQ=255)

                                                                          tGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGc              >  1:1891277/1‑93 (MQ=255)

                                                                          tGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGc              >  2:831905/1‑93 (MQ=255)

                                                                          tGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGc              >  2:831904/1‑93 (MQ=255)

                                                                           gCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCa             >  1:3907641/1‑93 (MQ=255)

                                                                            cTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCaa            <  2:733329/93‑1 (MQ=255)

                                                                              gagaCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgg          >  2:948916/1‑92 (MQ=255)

                                                                              gagaCTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgt          >  2:3988221/1‑93 (MQ=255)

                                                                               agaCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgtg         >  2:2373168/1‑93 (MQ=255)

                                                                                gaCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgtgt        <  2:4572950/93‑1 (MQ=255)

                                                                                gaCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgtgt        >  1:1036266/1‑93 (MQ=255)

                                                                                 aCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGtt       <  2:2507159/93‑1 (MQ=255)

                                                                                  cTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTg      >  2:430936/1‑93 (MQ=255)

                                                                                  cTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTg      <  2:2725416/93‑1 (MQ=255)

                                                                                  cTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCCGCGCACCGCGCAGCACCAGTTGTAACGGCGGCTCCTCCGCCAGTGTTg      >  1:1850713/1‑93 (MQ=255)

                                                                                   tttGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg     >  2:2192318/1‑93 (MQ=255)

                                                                                    ttGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCCCCTCCGCAAGTGTTGGc    >  1:3488102/1‑93 (MQ=255)

                                                                                      ggTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCag  >  1:3402458/1‑93 (MQ=255)

TGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAG  >  NC_000913/2327597‑2327775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`