Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,393,734 A→G G61G (GGT→GGC)  nuoJ ← NADH dehydrogenase I chain J

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,393,7340AG99.2% 404.7 / ‑4.6 129G61G (GGT→GGC) nuoJNADH dehydrogenase I chain J
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (69/59);  total (70/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 140 total aligned reads displayed.

CTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCACCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAG  >  NC_000913/2393644‑2393823
                                                                                          |                                                                                         
cTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGcc                                                                                         >  2:1965538/1‑93 (MQ=255)
cTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACCGACACGAACAGCACCATAATAGCGcc                                                                                         >  2:1317271/1‑93 (MQ=255)
 tGCGTTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGccc                                                                                        <  2:2121005/93‑1 (MQ=255)
  gCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCcg                                                                                       <  2:3816674/93‑1 (MQ=255)
    ggTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCcgcg                                                                                     >  2:3618008/1‑93 (MQ=255)
     gTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGt                                                                                    <  1:1062243/93‑1 (MQ=255)
     gTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGt                                                                                    <  1:1909633/93‑1 (MQ=255)
     gTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGt                                                                                    <  1:1909634/93‑1 (MQ=255)
     gTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCAACCCGAACACGAACAGCACCATAATAGCGCCCGCGt                                                                                    >  1:2575197/1‑93 (MQ=255)
      tttCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTa                                                                                   >  1:1420722/1‑93 (MQ=255)
       ttCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAg                                                                                  <  1:3931624/93‑1 (MQ=255)
        tCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGa                                                                                 <  1:4140325/93‑1 (MQ=255)
         cAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGAc                                                                                >  2:2172307/1‑93 (MQ=255)
          aGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACg                                                                               >  2:2177714/1‑93 (MQ=255)
          aGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACg                                                                               >  1:2651253/1‑93 (MQ=255)
           gCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGa                                                                              >  1:4594814/1‑93 (MQ=255)
           gCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCACCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGa                                                                              >  2:3868588/1‑93 (MQ=255)
             cACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATC‑TTAACACGGACCCGAAACGGACCATAAATGCGCCCGCGTAGACGATaa                                                                           >  2:790249/1‑93 (MQ=255)
             cACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATC‑TCACCACGAACACGAACAGCACCATAATGGAGCCCGCGTAGACGATaa                                                                           >  2:790250/1‑93 (MQ=255)
              aCTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATaa                                                                           <  2:3989226/93‑1 (MQ=255)
              aCTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATaa                                                                           >  2:1492587/1‑93 (MQ=255)
               cTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAAt                                                                          <  1:4551345/93‑1 (MQ=255)
                tGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCACCCGCGTAGACGATAAtt                                                                         >  2:1556244/1‑93 (MQ=255)
                 ggCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAAttt                                                                        <  1:2281897/93‑1 (MQ=255)
                  gCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTc                                                                       <  2:3719029/93‑1 (MQ=255)
                    gTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCa                                                                     >  1:2150356/1‑93 (MQ=255)
                    gTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCa                                                                     >  1:4672253/1‑93 (MQ=255)
                    gTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCa                                                                     <  1:2928350/93‑1 (MQ=255)
                     ttCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAg                                                                    <  1:3443648/93‑1 (MQ=255)
                     ttCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAg                                                                    <  1:4410458/93‑1 (MQ=255)
                      tCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAgc                                                                   >  1:225001/1‑93 (MQ=255)
                      tCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAgc                                                                   <  2:836293/93‑1 (MQ=255)
                         tGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCa                                                                >  1:2541202/1‑93 (MQ=255)
                            tCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCg                                                             >  1:1336250/1‑93 (MQ=255)
                             cGATTTCTGAATCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCgg                                                            <  2:1661164/93‑1 (MQ=255)
                               aTTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCg                                                          >  1:1421943/1‑93 (MQ=255)
                                tttCTGAACCTCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGTCGCCCGCGTAGACGATAATTTCCAGCGCACCTGCGa                                                         >  2:4199337/1‑93 (MQ=255)
                                tttCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGa                                                         >  2:369850/1‑93 (MQ=255)
                                tttCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGa                                                         >  1:1076342/1‑93 (MQ=255)
                                tttCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGa                                                         <  2:4267200/93‑1 (MQ=255)
                                  tCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAg                                                       <  2:3017580/93‑1 (MQ=255)
                                    tGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTa                                                     >  1:1351745/1‑93 (MQ=255)
                                     gAACCGCCCCGGTTGAGCATCACCCCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTaa                                                    <  2:4695199/93‑1 (MQ=255)
                                     gAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTaa                                                    <  1:2094634/93‑1 (MQ=255)
                                     gAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTaa                                                    >  1:3486706/1‑93 (MQ=255)
                                      aaCCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAg                                                   <  1:2483876/93‑1 (MQ=255)
                                      aaCCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAg                                                   >  1:846398/1‑93 (MQ=255)
                                      aaCCGCCCAGGTTGAGCATCAGCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAg                                                   <  2:219922/93‑1 (MQ=255)
                                       aCCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAgc                                                  >  1:4685790/1‑93 (MQ=255)
                                         cGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAgcgc                                                >  1:3194057/1‑93 (MQ=255)
                                           cccAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGccc                                              <  2:590105/93‑1 (MQ=255)
                                           cccAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGccc                                              <  2:4328395/93‑1 (MQ=255)
                                            ccAGGTTGAGTATCATTACCACGAACACGAACACCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCGCCGGCGAAGTAAGCGCCCa                                             <  2:2292478/93‑1 (MQ=255)
                                             cAGGTTGAGCATCATCAGCACGAGCACGAACAGCACCATAATGGCGCCCGCGGAGACGATAATTTCGAGCGCACCGGCGAAGAAAGAGCCCAg                                            >  2:2967158/1‑93 (MQ=255)
                                             cAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAg                                            >  1:3851909/1‑93 (MQ=255)
                                              aGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGt                                           <  1:1782709/93‑1 (MQ=255)
                                              aGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGt                                           <  2:2020536/93‑1 (MQ=255)
                                               ggTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTg                                          >  1:2918146/1‑93 (MQ=255)
                                                gTTGAGCATCATCACCACGAACACGAACAGCAGCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCGCCGGCGAAGTAAGCGCCCAGTga                                         <  1:467344/93‑1 (MQ=255)
                                                   gAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGagaa                                      >  1:4308948/1‑93 (MQ=255)
                                                    aGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGagaag                                     <  1:3165081/93‑1 (MQ=255)
                                                     gCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGagaaga                                    >  1:3706145/1‑93 (MQ=255)
                                                     gCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGagaaga                                    >  2:3999810/1‑93 (MQ=255)
                                                      catcatCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAgaagaa                                   >  1:726589/1‑93 (MQ=255)
                                                       atcatcACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAac                                  <  2:2388824/93‑1 (MQ=255)
                                                       atcatcACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAac                                  <  2:263636/93‑1 (MQ=255)
                                                        tcatcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAaca                                 <  1:4401136/93‑1 (MQ=255)
                                                        tcatcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAaca                                 >  2:2966910/1‑93 (MQ=255)
                                                         catcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAacac                                <  2:3017213/93‑1 (MQ=255)
                                                         catcaCCACGAACACGAACAGCACCATAATAGCGCCCGCGTAGACGATAATTTCCAGTGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAacac                                >  2:4153888/1‑93 (MQ=255)
                                                           tcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACAccc                              <  1:409945/93‑1 (MQ=255)
                                                           tcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACAccc                              >  1:624144/1‑93 (MQ=255)
                                                           ccaccacGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACcccc                              <  2:875003/92‑1 (MQ=255)
                                                             accacGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCg                            >  1:1202266/1‑93 (MQ=255)
                                                                acgaacacgaacAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGaa                         >  2:3282349/1‑93 (MQ=255)
                                                                 cgaacacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGaaa                        <  2:1295370/93‑1 (MQ=255)
                                                                  gaacacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAAt                       >  1:1745561/1‑93 (MQ=255)
                                                                   aacacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATc                      >  1:1088069/1‑93 (MQ=255)
                                                                    acacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCg                     >  2:4141404/1‑93 (MQ=255)
                                                                    acacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCg                     >  2:1860651/1‑93 (MQ=255)
                                                                      acgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGcc                   >  1:438211/1‑93 (MQ=255)
                                                                      acgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGcc                   >  2:3336119/1‑93 (MQ=255)
                                                                       cgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCca                  <  2:3637163/93‑1 (MQ=255)
                                                                        gaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCcag                 <  2:2885967/93‑1 (MQ=255)
                                                                        gaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCcag                 <  1:3631709/93‑1 (MQ=255)
                                                                          acaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGAACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCcagca               <  1:4296485/93‑1 (MQ=255)
                                                                           caGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCcagcag              >  1:594089/1‑93 (MQ=255)
                                                                           caGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCcagcag              <  1:4021418/93‑1 (MQ=255)
                                                                           caGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCcagcag              >  2:631651/1‑93 (MQ=255)
                                                                            aGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCagcagc             >  1:3762887/1‑93 (MQ=255)
                                                                            aGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCagcagc             >  1:1814037/1‑93 (MQ=255)
                                                                             gCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCg            >  2:4263365/1‑93 (MQ=255)
                                                                               aCCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGaa          >  2:3702408/1‑93 (MQ=255)
                                                                                 cATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAaat        <  2:3467921/93‑1 (MQ=255)
                                                                                  aTAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAaata       <  1:267653/93‑1 (MQ=255)
                                                                                   tAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAaataa      >  1:2423189/1‑93 (MQ=255)
                                                                                   tAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAaataa      >  1:3215841/1‑93 (MQ=255)
                                                                                    aaTGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAaataat     <  1:831744/93‑1 (MQ=255)
                                                                                    aaTGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAaataat     >  2:1521437/1‑93 (MQ=255)
                                                                                      tGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCa   >  2:2795217/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCACCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAG  >  NC_000913/2393644‑2393823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑