BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,413,512	T→C	I249T (ATC→ACC)	pta →	phosphotransacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,413,512	0	T	C	96.4%	327.5 / 1.6	111	I249T (ATC→ACC)	pta	phosphotransacetylase
Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (47/59); total (50/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 127 total aligned reads displayed.

CTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGA  >  NC_000913/2413425‑2413601

cTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATa                                                                                      <  2:696547/93‑1 (MQ=255)

cTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATa                                                                                      <  2:512708/93‑1 (MQ=255)

cTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATa                                                                                      <  2:512707/93‑1 (MQ=255)

 tgtgCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAacac                                                                                     >  1:1883276/1‑93 (MQ=255)

  gtgCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATaat                                                                                    >  2:3946551/1‑91 (MQ=255)

   tgCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTc                                                                                   <  2:4394643/93‑1 (MQ=255)

    gCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTcg                                                                                  <  1:3177441/93‑1 (MQ=255)

    gCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTcg                                                                                  <  1:3010269/93‑1 (MQ=255)

      cGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTcgcc                                                                                >  1:2507696/1‑93 (MQ=255)

      cGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAAAACTcgcc                                                                                >  1:4588302/1‑93 (MQ=255)

       gTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACCAAGGCGGAACCAATACTcgccc                                                                               >  2:453051/1‑92 (MQ=255)

        tGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTcgccgc                                                                              >  2:4030534/1‑93 (MQ=255)

        tGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACAAAAGCGACACCAATACTcgccgc                                                                              >  2:3221748/1‑93 (MQ=255)

         ggAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCcgcg                                                                             >  1:3701887/1‑93 (MQ=255)

           aGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGtt                                                                           <  2:1978207/93‑1 (MQ=255)

           aGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCGCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGtt                                                                           <  2:1978206/93‑1 (MQ=255)

            gCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTa                                                                          <  2:1892864/93‑1 (MQ=255)

              tttGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGCATGGGAACATCATCAACGAATTCGACACCAATAATCTCCGCGTTaaa                                                                        >  2:3266896/1‑93 (MQ=255)

              tttGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTaaa                                                                        >  2:3266895/1‑93 (MQ=255)

              tttGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTaaa                                                                        <  2:4633501/93‑1 (MQ=255)

              tttGACCTGAGCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTaaa                                                                        <  1:2174339/93‑1 (MQ=255)

               ttGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCCATAATCGCCCCGTTAAAt                                                                       >  2:2514554/1‑93 (MQ=255)

                 gACCTGATCGCGACTCGTGCGATCGATATGGCTCCCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAAAACTCGCCGCGGTAAATcc                                                                     >  2:1706590/1‑93 (MQ=255)

                   ccTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGCAGGCGACACCAATACTCGCCGCGTTTAATCCGt                                                                   >  2:4690518/1‑93 (MQ=255)

                   ccTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGt                                                                   <  1:56426/93‑1 (MQ=255)

                   ccTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGt                                                                   <  2:3963605/93‑1 (MQ=255)

                    cTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTc                                                                  >  1:3169392/1‑93 (MQ=255)

                     tGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTTCCCGCGGTAAATCCgctc                                                                 >  2:860480/1‑90 (MQ=255)

                     cgATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCa                                                                 <  1:1543848/92‑1 (MQ=255)

                       aTCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGGGACACCAATACTCGCCGCGTTAAATCCGTCACt                                                               >  1:296084/1‑93 (MQ=255)

                          ggcaCTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACG‑AGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCt                                                           <  2:661928/90‑1 (MQ=255)

                          gcgACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACtttt                                                            >  2:3766930/1‑92 (MQ=255)

                           cgACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCt                                                           <  2:3879717/93‑1 (MQ=255)

                           cgACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCt                                                           <  2:662062/93‑1 (MQ=255)

                             aCTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTgc                                                         <  1:3781070/93‑1 (MQ=255)

                             aCTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTgc                                                         <  1:3582262/93‑1 (MQ=255)

                               tCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTgcgc                                                       >  1:1365806/1‑93 (MQ=255)

                                cGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGcgca                                                      <  2:1669014/93‑1 (MQ=255)

                                cGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGcgca                                                      <  2:3032816/93‑1 (MQ=255)

                                 gTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCCTCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGcgcac                                                     >  1:220447/1‑93 (MQ=255)

                                 gTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTTTTcgcac                                                     >  2:1740264/1‑93 (MQ=255)

                                  tGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGcgcacg                                                    >  1:3986370/1‑93 (MQ=255)

                                    cgatcgatATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATCCTCGCCGCGTTAAATCCGTCACTTTCTGcgcacgca                                                  <  2:3243212/93‑1 (MQ=255)

                                    cgatcgatATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGcgcacgca                                                  >  1:378468/1‑93 (MQ=255)

                                     gatcgatATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACgcag                                                 >  1:2762641/1‑93 (MQ=255)

                                     gatcgatATGGCTCGCCACCTGAATGCGACCAACATCAACGAAGGCGAAACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACgcag                                                 >  2:2285445/1‑93 (MQ=255)

                                      atcgatATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACgcagc                                                >  2:694256/1‑93 (MQ=255)

                                       tcgatATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACgcagca                                               <  2:886859/93‑1 (MQ=255)

                                       tcgatATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACgcagca                                               >  2:4166841/1‑93 (MQ=255)

                                       tccatatGGCTCCCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACgcagca                                               <  2:3755285/90‑1 (MQ=255)

                                        cgatATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCAt                                              <  1:702096/93‑1 (MQ=255)

                                          atatGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTc                                            >  2:3953201/1‑93 (MQ=255)

                                           tatGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTcc                                           <  2:926075/93‑1 (MQ=255)

                                                cTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGcaca                                      >  2:4063809/1‑93 (MQ=255)

                                                 tCGCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACAt                                     <  1:818643/93‑1 (MQ=255)

                                                   gCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGc                                   <  1:1326715/93‑1 (MQ=255)

                                                   gCCACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGc                                   >  2:661553/1‑93 (MQ=255)

                                                    ccACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCt                                  >  1:2041931/1‑93 (MQ=255)

                                                     cACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTg                                 <  2:4235686/93‑1 (MQ=255)

                                                     cACCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTg                                 >  2:166867/1‑93 (MQ=255)

                                                      aCCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTgg                                >  2:2941929/1‑93 (MQ=255)

                                                      aCCTGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTgg                                >  1:209986/1‑93 (MQ=255)

                                                         tGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGc                             >  2:4528152/1‑93 (MQ=255)

                                                         tGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGc                             <  2:2831874/93‑1 (MQ=255)

                                                         tGAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGc                             <  1:3474231/93‑1 (MQ=255)

                                                          gAATGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCa                            >  1:968222/1‑93 (MQ=255)

                                                           aaTGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCAc                           >  2:3426168/1‑93 (MQ=255)

                                                            aTGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACt                          <  2:3315460/93‑1 (MQ=255)

                                                             tGCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACtt                         >  1:442188/1‑93 (MQ=255)

                                                              gCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTc                        >  1:3458101/1‑93 (MQ=255)

                                                              gCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTc                        <  2:663612/93‑1 (MQ=255)

                                                              gCGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTc                        <  2:2231230/93‑1 (MQ=255)

                                                               cGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTcc                       <  2:3634285/93‑1 (MQ=255)

                                                               cGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTcc                       <  1:648028/93‑1 (MQ=255)

                                                               cGACCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTcc                       <  2:1384945/93‑1 (MQ=255)

                                                                 aCCATCATCAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGt                     >  2:1198753/1‑93 (MQ=255)

                                                                    atcatcAACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGcc                  <  1:367576/93‑1 (MQ=255)

                                                                     tcatcaACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCg                 >  2:765986/1‑93 (MQ=255)

                                                                       atcaACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGt               >  1:2514262/1‑93 (MQ=255)

                                                                        tcaACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGtt              <  2:502515/93‑1 (MQ=255)

                                                                        tcaACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACACGCTGGAGCACTTCCGTGCCGGtt              >  1:1786623/1‑93 (MQ=255)

                                                                         caACGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTtc             >  1:3059462/1‑93 (MQ=255)

                                                                          aaCGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTtct            >  2:877025/1‑93 (MQ=255)

                                                                           aCGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTtctc           >  1:1098819/1‑93 (MQ=255)

                                                                           aCGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTtctc           >  1:3946899/1‑93 (MQ=255)

                                                                            cGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTctct          >  1:1671345/1‑93 (MQ=255)

                                                                            cGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTctct          <  2:2507696/93‑1 (MQ=255)

                                                                            cGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTctct          >  2:1467635/1‑93 (MQ=255)

                                                                             gAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTctg         >  1:745482/1‑93 (MQ=255)

                                                                              aaGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTctgc        >  1:2355826/1‑93 (MQ=255)

                                                                              aaGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTctgc        <  2:1226586/93‑1 (MQ=255)

                                                                               aGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTctgct       >  1:1440725/1‑93 (MQ=255)

                                                                                ggCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTctgctg      >  2:2115127/1‑93 (MQ=255)

                                                                                ggCGACACCAATACTCGCCGCATTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTctgctg      <  2:2104507/93‑1 (MQ=255)

                                                                                ggCGACAACAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTctgctg      >  2:3697758/1‑93 (MQ=255)

                                                                                  cGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGt    <  1:3389635/93‑1 (MQ=255)

                                                                                  cGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGt    <  1:3979725/93‑1 (MQ=255)

                                                                                    acacCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTTGTGa  >  1:565442/1‑93 (MQ=255)

                                                                                    acacCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGa  >  1:3203746/1‑93 (MQ=255)

                                                                                    acacCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGa  >  1:414963/1‑93 (MQ=255)

CTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGA  >  NC_000913/2413425‑2413601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`