Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,438,697 A→C L310R (CTG→CGG)  fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,438,6970AC99.2% 375.8 / ‑4.7 117L310R (CTG→CGG) fabB3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (57/59);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

Only 100 of 122 total aligned reads displayed.

GCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATG  >  NC_000913/2438609‑2438786
                                                                                        |                                                                                         
gCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGctc                                                                                       >  1:3346476/1‑93 (MQ=255)
 ccAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGctct                                                                                      <  1:2645858/93‑1 (MQ=255)
  cagcagCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGCTAGCTGCCCGctctc                                                                                     >  1:2115871/1‑92 (MQ=255)
   agcagcGCGCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCttt                                                                                    <  1:4584388/93‑1 (MQ=255)
   agcagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCttt                                                                                    <  1:665992/93‑1 (MQ=255)
   agcagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCttt                                                                                    <  1:4584389/93‑1 (MQ=255)
   agcagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCttt                                                                                    <  1:447890/93‑1 (MQ=255)
   agcagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCttt                                                                                    <  1:4172396/93‑1 (MQ=255)
    gcagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCttta                                                                                   >  1:2528696/1‑92 (MQ=255)
    gcagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTc                                                                                   <  1:2425886/93‑1 (MQ=255)
     cagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCa                                                                                  <  1:1143538/93‑1 (MQ=255)
      agcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCAc                                                                                 >  1:2059556/1‑93 (MQ=255)
      agcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCAc                                                                                 <  1:1621955/93‑1 (MQ=255)
      agcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCAc                                                                                 <  1:3354801/93‑1 (MQ=255)
       gcgcCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACg                                                                                <  2:2342243/93‑1 (MQ=255)
         gcCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTc                                                                              <  1:1141790/93‑1 (MQ=255)
           ccAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGc                                                                            >  1:1314108/1‑93 (MQ=255)
             agagagTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCa                                                                          >  2:4690162/1‑93 (MQ=255)
               agagTGCCGTGTCATGACTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGACCACTTCCCGGATAGCTGCCCGCTCTTTCACGTCGCCAAc                                                                        <  2:1815631/93‑1 (MQ=255)
                  gTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCgg                                                                     >  1:3635881/1‑93 (MQ=255)
                  gTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCgg                                                                     >  1:3635882/1‑93 (MQ=255)
                   tGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGa                                                                    <  1:1491864/93‑1 (MQ=255)
                   tGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGa                                                                    <  1:3130228/93‑1 (MQ=255)
                     gccGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGt                                                                  <  1:2343826/92‑1 (MQ=255)
                     aCCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAgc                                                                  >  2:1117159/1‑92 (MQ=255)
                     aCCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAgc                                                                  >  2:730627/1‑92 (MQ=255)
                     aCCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGt                                                                  <  2:2970565/93‑1 (MQ=255)
                      ccGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTc                                                                 <  2:2183409/93‑1 (MQ=255)
                      ccGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTc                                                                 <  2:2982103/93‑1 (MQ=255)
                         gTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGaa                                                              <  2:1345509/93‑1 (MQ=255)
                          tCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAg                                                             >  1:3808271/1‑93 (MQ=255)
                           cATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGt                                                            >  2:3839398/1‑93 (MQ=255)
                             tGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTAc                                                          <  1:4240913/93‑1 (MQ=255)
                              tgCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc                                                         <  2:1159711/92‑1 (MQ=255)
                               gCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCg                                                        >  1:4048187/1‑93 (MQ=255)
                                cTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGTAGTCGAAGTACCGt                                                       >  2:1408408/1‑93 (MQ=255)
                                 tttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTg                                                      <  2:584811/93‑1 (MQ=255)
                                 tttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTg                                                      >  1:408056/1‑93 (MQ=255)
                                  ttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTgg                                                     >  2:2044092/1‑93 (MQ=255)
                                    ggTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGa                                                   <  2:2016995/93‑1 (MQ=255)
                                    ggTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGa                                                   <  1:4541634/93‑1 (MQ=255)
                                    gcgtGCAGAACTCGCCGGGCGCTTATCGCCGAACACTTCGCGGATAGCTGCCCGCTCTTTCACGGCTCCAACCGGAGTCGAAGTACCGGGGGa                                                   <  2:2939125/90‑1 (MQ=255)
                                       tGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGtt                                                >  2:2041443/1‑93 (MQ=255)
                                        gCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTc                                               >  2:2461458/1‑93 (MQ=255)
                                         cAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCa                                              <  2:908848/93‑1 (MQ=255)
                                          aGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGCGTTCAg                                             >  2:2377539/1‑93 (MQ=255)
                                          aGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAg                                             <  2:3753075/93‑1 (MQ=255)
                                           gAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAgg                                            >  1:4163895/1‑93 (MQ=255)
                                             aaTCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTa                                          >  1:3782898/1‑93 (MQ=255)
                                             aaTCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTa                                          >  2:3838177/1‑93 (MQ=255)
                                               tCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAAt                                        <  1:4706514/93‑1 (MQ=255)
                                               tCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAAt                                        <  2:4100469/93‑1 (MQ=255)
                                                 gCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCg                                      >  2:1714359/1‑93 (MQ=255)
                                                  ccGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTGATCGa                                     <  2:3914951/93‑1 (MQ=255)
                                                  ccGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTCATCgt                                     >  1:2284360/1‑92 (MQ=255)
                                                  ccGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGa                                     <  1:4149313/93‑1 (MQ=255)
                                                  ccGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAAACGGAGTCGAAGTACCGTGGGAGGTCGGATAATCGa                                     >  2:3739912/1‑93 (MQ=255)
                                                    gggCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGAtt                                   >  1:2279759/1‑93 (MQ=255)
                                                     ggCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTg                                  <  2:2583644/93‑1 (MQ=255)
                                                     ggCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTg                                  >  1:1391525/1‑93 (MQ=255)
                                                        tctTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTgggg                               <  2:1383338/93‑1 (MQ=255)
                                                         ctTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCG‑GGGAGTTCAGGTAATCGATTGGGGtt                             >  1:698362/1‑92 (MQ=255)
                                                            aTCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATc                           <  2:3713280/93‑1 (MQ=255)
                                                             tCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCa                          >  1:3600735/1‑93 (MQ=255)
                                                              cgacGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCaa                         <  2:1221049/90‑1 (MQ=255)
                                                              cGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCaa                         <  2:2528539/93‑1 (MQ=255)
                                                              cGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAATACCGTGGGAGTTCAGGTAATCGATTGGGGTATCaa                         <  2:2528540/93‑1 (MQ=255)
                                                               gCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAAc                        >  2:4114786/1‑93 (MQ=255)
                                                                ccGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACg                       >  1:4548704/1‑93 (MQ=255)
                                                                  gAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGcc                     <  1:3829252/93‑1 (MQ=255)
                                                                  gAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGcc                     >  2:1021440/1‑93 (MQ=255)
                                                                   aaCACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGccc                    >  1:359731/1‑92 (MQ=255)
                                                                   aaCACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCa                    <  1:2458325/93‑1 (MQ=255)
                                                                   aaCACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCa                    >  1:1194064/1‑93 (MQ=255)
                                                                   aaCACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCa                    <  1:4275023/93‑1 (MQ=255)
                                                                   aaCACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCa                    <  2:2538092/93‑1 (MQ=255)
                                                                   aaCACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCa                    >  1:359732/1‑93 (MQ=255)
                                                                   aaCACTTCACGGATAGCTGCACGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCa                    <  2:4729548/93‑1 (MQ=255)
                                                                    acacTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCAt                   <  1:2740878/93‑1 (MQ=255)
                                                                     cacTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATg                  >  1:3548236/1‑93 (MQ=255)
                                                                      acTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGc                 >  1:2043483/1‑93 (MQ=255)
                                                                      acTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGc                 <  1:3687158/93‑1 (MQ=255)
                                                                      acTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGc                 <  1:3687318/93‑1 (MQ=255)
                                                                      acTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGc                 >  1:2043482/1‑93 (MQ=255)
                                                                       cTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCa                >  1:639632/1‑93 (MQ=255)
                                                                           aCGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGc            >  2:2388984/1‑93 (MQ=255)
                                                                                tAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCt       >  2:3446502/1‑93 (MQ=255)
                                                                                tAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCt       <  2:4570839/93‑1 (MQ=255)
                                                                                tAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGACATGCATCGCCATCt       >  2:4454283/1‑93 (MQ=255)
                                                                                  gCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTc     <  1:3084535/93‑1 (MQ=255)
                                                                                   cTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCa    >  2:1144482/1‑93 (MQ=255)
                                                                                   cTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCa    <  2:819669/93‑1 (MQ=255)
                                                                                   cTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCa    >  1:3144755/1‑93 (MQ=255)
                                                                                   cTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCa    >  1:1410733/1‑93 (MQ=255)
                                                                                    tGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCAt   >  2:4378902/1‑93 (MQ=255)
                                                                                    tGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCAt   <  1:1516024/93‑1 (MQ=255)
                                                                                    tGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGCAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCAt   >  2:2651233/1‑93 (MQ=255)
                                                                                    cgCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCAt   <  1:405140/92‑1 (MQ=255)
                                                                                     gCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATg  >  1:1170262/1‑93 (MQ=255)
                                                                                     gCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATg  >  1:1983446/1‑93 (MQ=255)
                                                                                        |                                                                                         
GCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATG  >  NC_000913/2438609‑2438786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑