BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,461,706	T→C	S225S (AGT→AGC)	b2345 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,461,706	0	T	C	99.0%	668.4 / ‑3.2	205	S225S (AGT→AGC)	b2345	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (113/90); total (114/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01

Only 100 of 208 total aligned reads displayed.

TTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGT  >  NC_000913/2461616‑2461795

ttACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGa                                                                                         >  1:4593096/1‑93 (MQ=255)

ttACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGa                                                                                         <  1:2909311/93‑1 (MQ=255)

 tACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaa                                                                                        >  1:2332267/1‑93 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  1:412387/93‑1 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  1:2459800/93‑1 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  2:3184218/93‑1 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAAGTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  1:2785948/93‑1 (MQ=255)

    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAc                                                                                     <  1:1366069/93‑1 (MQ=255)

    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAc                                                                                     <  1:3671182/93‑1 (MQ=255)

     tATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAcc                                                                                    >  2:1489915/1‑92 (MQ=255)

     tATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACg                                                                                    >  1:1306292/1‑93 (MQ=255)

     tATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACg                                                                                    >  1:726787/1‑93 (MQ=255)

     tATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACg                                                                                    <  1:2674083/93‑1 (MQ=255)

     tATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACg                                                                                    >  1:1381061/1‑93 (MQ=255)

      aTCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGc                                                                                   >  1:4080392/1‑93 (MQ=255)

      aTCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGc                                                                                   >  1:4507224/1‑93 (MQ=255)

       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGATGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGcc                                                                                  >  2:4306743/1‑93 (MQ=255)

        ctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCa                                                                                 >  1:4625666/1‑93 (MQ=255)

        ctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGCAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAATCGCCa                                                                                 <  2:4709564/93‑1 (MQ=255)

           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              <  2:4435015/93‑1 (MQ=255)

           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              >  1:1038524/1‑93 (MQ=255)

           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              >  2:2545040/1‑93 (MQ=255)

           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              >  2:3928616/1‑93 (MQ=255)

           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              >  2:310502/1‑93 (MQ=255)

             ggTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAgg                                                                            >  2:2073682/1‑92 (MQ=255)

             ggTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGt                                                                            <  1:4302196/93‑1 (MQ=255)

              gTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTg                                                                           >  1:2731112/1‑93 (MQ=255)

              gTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTg                                                                           >  2:1019956/1‑93 (MQ=255)

               ttACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGc                                                                          <  1:3009090/93‑1 (MQ=255)

               ttACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGc                                                                          >  1:1326249/1‑93 (MQ=255)

                tactacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCt                                                                         >  1:1844146/1‑93 (MQ=255)

                  ctacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTc                                                                       >  2:2844781/1‑93 (MQ=255)

                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      >  1:3494738/1‑93 (MQ=255)

                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGAGCTTCa                                                                      >  2:1170231/1‑93 (MQ=255)

                    acGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAg                                                                     >  1:3930689/1‑93 (MQ=255)

                    acGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAg                                                                     <  1:2598406/93‑1 (MQ=255)

                     cGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGc                                                                    <  1:3738598/93‑1 (MQ=255)

                     cGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGc                                                                    <  2:223650/93‑1 (MQ=255)

                      gATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCg                                                                   <  1:147779/93‑1 (MQ=255)

                        tCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTg                                                                 <  2:1433263/93‑1 (MQ=255)

                         cAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTgg                                                                <  2:785422/93‑1 (MQ=255)

                          aGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGt                                                               <  2:505168/93‑1 (MQ=255)

                           ggACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGtt                                                              <  2:4200604/93‑1 (MQ=255)

                              cgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGa                                                           <  2:664873/93‑1 (MQ=255)

                              cgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGa                                                           <  2:2665210/93‑1 (MQ=255)

                                acTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaaa                                                         >  2:4495190/1‑93 (MQ=255)

                                acTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaaa                                                         >  1:3290383/1‑93 (MQ=255)

                                  tACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGc                                                       <  1:1636090/93‑1 (MQ=255)

                                  tACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGc                                                       >  2:4043232/1‑93 (MQ=255)

                                   aCCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCa                                                      <  2:1734491/93‑1 (MQ=255)

                                   aCCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTAAGCGTGGTTTGAAAGCa                                                      >  1:251474/1‑93 (MQ=255)

                                     cacaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAga                                                    >  1:3312644/1‑92 (MQ=255)

                                       caTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaa                                                  <  1:541028/93‑1 (MQ=255)

                                        aTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaaa                                                 <  1:1987621/93‑1 (MQ=255)

                                        aTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaaa                                                 <  2:2296559/93‑1 (MQ=255)

                                         ttCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAg                                                >  1:1577714/1‑93 (MQ=255)

                                         atCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAg                                                <  1:3134738/92‑1 (MQ=255)

                                              agagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATc                                           <  1:3066442/93‑1 (MQ=255)

                                              agagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATc                                           >  1:4235987/1‑93 (MQ=255)

                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          >  2:3848220/1‑93 (MQ=255)

                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          >  2:3758925/1‑93 (MQ=255)

                                                agGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCAt                                         <  1:1682597/93‑1 (MQ=255)

                                                 ggAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATg                                        >  1:1743998/1‑93 (MQ=255)

                                                  gAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTCTGAAAGCAGTAAAGCTATCATgg                                       >  2:3382462/1‑93 (MQ=255)

                                                   aaTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGc                                      >  2:3383870/1‑93 (MQ=255)

                                                   aaTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGc                                      >  2:2132207/1‑93 (MQ=255)

                                                        gTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAt                                 >  2:10657/1‑93 (MQ=255)

                                                         tATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATc                                >  1:1965904/1‑93 (MQ=255)

                                                          aTTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCa                               >  2:1560026/1‑93 (MQ=255)

                                                          aTTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGAAGAATCa                               >  1:4381435/1‑93 (MQ=255)

                                                           ttAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAt                              >  1:221255/1‑93 (MQ=255)

                                                            tAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAtt                             <  2:280822/93‑1 (MQ=255)

                                                             aaTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAttt                            <  2:3064396/93‑1 (MQ=255)

                                                              aTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTg                           <  1:4744697/93‑1 (MQ=255)

                                                              aTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTg                           <  2:1674561/93‑1 (MQ=255)

                                                               tGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGc                          <  2:3465655/93‑1 (MQ=255)

                                                                   gATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACt                      >  2:4241945/1‑93 (MQ=255)

                                                                      aGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGt                   >  2:3269295/1‑93 (MQ=255)

                                                                       gTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGtt                  >  1:4708173/1‑93 (MQ=255)

                                                                         ctcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTcc                <  2:289452/93‑1 (MQ=255)

                                                                         ctcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTcc                <  1:3594866/93‑1 (MQ=255)

                                                                           cACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGa              >  2:3028378/1‑93 (MQ=255)

                                                                            aCCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGat             >  1:717695/1‑93 (MQ=255)

                                                                            aCCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGat             >  2:1959439/1‑93 (MQ=255)

                                                                             cccAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatc            <  1:676626/93‑1 (MQ=255)

                                                                              ccAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatca           >  1:1738952/1‑93 (MQ=255)

                                                                                 atatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGAtcatca        <  2:1691285/93‑1 (MQ=255)

                                                                                 atatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGAtcatca        <  1:884528/93‑1 (MQ=255)

                                                                                 atatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGAtcatca        >  1:3254831/1‑93 (MQ=255)

                                                                                  tatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAg       <  2:411882/93‑1 (MQ=255)

                                                                                   atTGTCGCGAAAACGCCAAGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAgg      >  2:4645108/1‑93 (MQ=255)

                                                                                    ttGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGt     >  1:3963260/1‑93 (MQ=255)

                                                                                    ttGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGt     >  2:1105184/1‑93 (MQ=255)

                                                                                    ttGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGt     <  2:1264016/93‑1 (MQ=255)

                                                                                     tGTAGCGAAAACGCCAGTAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGtt    <  2:3608776/93‑1 (MQ=255)

                                                                                     tGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGtt    <  2:1375500/93‑1 (MQ=255)

                                                                                     tGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGtt    >  2:1351066/1‑93 (MQ=255)

                                                                                     tGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGtt    <  1:2651831/93‑1 (MQ=255)

                                                                                     tGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGtt    <  2:2576599/93‑1 (MQ=255)

                                                                                       tAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGt  >  2:3818996/1‑93 (MQ=255)

TTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGT  >  NC_000913/2461616‑2461795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`