BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,464,010	T→C	H230H (CAT→CAC)	yfdC →	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,464,010	0	T	C	97.9%	444.7 / ‑1.9	141	H230H (CAT→CAC)	yfdC	putative transport
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (77/61); total (78/63)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 146 total aligned reads displayed.

TATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCACTACCTAC  >  NC_000913/2463920‑2464099

tATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACACacat                                                                                         >  2:1770691/1‑93 (MQ=255)

 aTGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATc                                                                                        <  2:3278332/93‑1 (MQ=255)

 aTGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGAGTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATc                                                                                        >  2:2941421/1‑93 (MQ=255)

  tGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCg                                                                                       <  1:3823746/93‑1 (MQ=255)

   ggTTTGGATGTTACCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTAACACCACCCACATCGt                                                                                      <  2:4212189/93‑1 (MQ=255)

     tttGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTgg                                                                                    >  1:3467788/1‑93 (MQ=255)

       tGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTc                                                                                  <  1:2383540/93‑1 (MQ=255)

           tGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGtt                                                                              >  2:3531355/1‑93 (MQ=255)

           tGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGtt                                                                              >  1:3187504/1‑93 (MQ=255)

            gTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTc                                                                             >  2:478913/1‑93 (MQ=255)

            gTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTc                                                                             <  2:453365/93‑1 (MQ=255)

             tttCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCt                                                                            <  2:2237708/93‑1 (MQ=255)

             tttCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCt                                                                            <  2:2902759/93‑1 (MQ=255)

              ttCCTGCAGCAGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTg                                                                           <  2:1800393/93‑1 (MQ=255)

                 cTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTg                                                                        <  2:2193714/93‑1 (MQ=255)

                 cTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTg                                                                        >  2:4713810/1‑93 (MQ=255)

                 cTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTg                                                                        >  1:2236971/1‑93 (MQ=255)

                 cTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTACCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTg                                                                        <  1:2674106/93‑1 (MQ=255)

                   gCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGaa                                                                      >  1:488093/1‑93 (MQ=255)

                    cAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGaaa                                                                     <  2:1537315/93‑1 (MQ=255)

                    cAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGaaa                                                                     <  2:3669925/93‑1 (MQ=255)

                     aGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAAt                                                                    <  2:4043680/93‑1 (MQ=255)

                     aGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAAt                                                                    <  2:2061182/93‑1 (MQ=255)

                         ggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCctc                                                                <  1:295898/93‑1 (MQ=255)

                         ggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCctc                                                                >  1:1498158/1‑93 (MQ=255)

                         ggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCctc                                                                <  1:1800017/93‑1 (MQ=255)

                         ggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCctc                                                                >  2:1855020/1‑93 (MQ=255)

                            gCGGAAAAGTTTGT‑GTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATc                                                            <  2:4153672/93‑1 (MQ=255)

                             cGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATc                                                            >  2:1895361/1‑93 (MQ=255)

                               gCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTg                                                          >  2:160535/1‑93 (MQ=255)

                               gCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTg                                                          >  1:3689233/1‑93 (MQ=255)

                               gCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTg                                                          <  2:4450356/93‑1 (MQ=255)

                               gCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTg                                                          <  2:2723989/93‑1 (MQ=255)

                                cAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTgg                                                         <  1:4704494/93‑1 (MQ=255)

                                 aaaGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgt                                                        <  2:4350667/93‑1 (MQ=255)

                                 aaaGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgt                                                        >  2:229340/1‑93 (MQ=255)

                                  aaGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgtg                                                       <  2:1340316/93‑1 (MQ=255)

                                  aaGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgtg                                                       >  1:3975110/1‑93 (MQ=255)

                                  aaGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgtg                                                       >  2:3699510/1‑93 (MQ=255)

                                    gATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGtt                                                     >  2:4626/1‑93 (MQ=255)

                                      ttGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTa                                                   >  2:3684454/1‑93 (MQ=255)

                                      ttGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTa                                                   >  1:1075731/1‑93 (MQ=255)

                                      ttGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTa                                                   <  1:208367/93‑1 (MQ=255)

                                        gtggtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                                                 >  2:3516236/1‑93 (MQ=255)

                                        gtggtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                                                 <  2:1246539/93‑1 (MQ=255)

                                         tggtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACg                                                >  1:722009/1‑93 (MQ=255)

                                            tgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTa                                             <  2:651716/93‑1 (MQ=255)

                                            tgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTa                                             <  2:4373632/93‑1 (MQ=255)

                                              aTTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACg                                           <  1:3355255/93‑1 (MQ=255)

                                              aTTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACg                                           <  1:2648861/93‑1 (MQ=255)

                                               ttATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGc                                          <  1:1121307/93‑1 (MQ=255)

                                               ttATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGc                                          >  1:2847069/1‑93 (MQ=255)

                                               ttATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGc                                          >  2:1064440/1‑93 (MQ=255)

                                               ttATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGc                                          >  1:1232016/1‑93 (MQ=255)

                                                tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCt                                         >  1:2206005/1‑93 (MQ=255)

                                                tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTATATCTGGTGTTTAACGGTACGCt                                         <  2:3852546/93‑1 (MQ=255)

                                                  tatTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGc                                       <  2:4172321/93‑1 (MQ=255)

                                                  tatTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGc                                       >  1:1553133/1‑93 (MQ=255)

                                                  tatTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGc                                       >  1:2623302/1‑93 (MQ=255)

                                                   atTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCa                                      >  1:3494426/1‑93 (MQ=255)

                                                      gatgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTg                                   >  1:2680871/1‑93 (MQ=255)

                                                       atgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTgg                                  >  1:469206/1‑93 (MQ=255)

                                                       atgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTgg                                  <  1:2426542/93‑1 (MQ=255)

                                                        tgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGa                                 <  2:4324861/93‑1 (MQ=255)

                                                        tgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGa                                 <  2:4574570/93‑1 (MQ=255)

                                                        tgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGa                                 >  2:1203461/1‑93 (MQ=255)

                                                         gaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAg                                >  2:703478/1‑93 (MQ=255)

                                                          aCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGc                               <  2:3832155/93‑1 (MQ=255)

                                                           ccTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCg                              >  2:256373/1‑93 (MQ=255)

                                                            cTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGCGCGa                             >  2:1070522/1‑93 (MQ=255)

                                                            cTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGa                             >  1:476002/1‑93 (MQ=255)

                                                             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAt                            >  2:3936119/1‑93 (MQ=255)

                                                             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAt                            <  1:3296440/93‑1 (MQ=255)

                                                             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAt                            >  1:3826739/1‑93 (MQ=255)

                                                              ggCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAtt                           <  1:719189/93‑1 (MQ=255)

                                                                 ttATTCCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAtttaa                        <  2:3858682/93‑3 (MQ=255)

                                                                  tATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCAt                       >  1:4536981/1‑93 (MQ=255)

                                                                   aTTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATc                      >  1:104651/1‑93 (MQ=255)

                                                                   aTTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATCTCATc                      >  1:2758096/1‑93 (MQ=255)

                                                                    ttGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt                     <  1:2525136/93‑1 (MQ=255)

                                                                      gCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTgg                   <  2:1799354/93‑1 (MQ=255)

                                                                       cccTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGc                  >  1:2006609/1‑93 (MQ=255)

                                                                       cccTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGc                  <  2:1980182/93‑1 (MQ=255)

                                                                        ccagggTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGcc                 >  1:4315044/4‑93 (MQ=255)

                                                                        ccTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGcc                 >  1:4332507/1‑93 (MQ=255)

                                                                        ccTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGcc                 >  1:4317287/1‑93 (MQ=255)

                                                                        ccTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGcc                 >  1:646597/1‑93 (MQ=255)

                                                                         cTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGccc                >  2:2010321/1‑93 (MQ=255)

                                                                          tGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCt               >  2:1261791/1‑93 (MQ=255)

                                                                           gggTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCtt              >  1:2194250/1‑93 (MQ=255)

                                                                           gggTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCtt              >  1:1279715/1‑93 (MQ=255)

                                                                            ggTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTc             <  1:392869/93‑1 (MQ=255)

                                                                            ggTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTc             >  1:3710357/1‑93 (MQ=255)

                                                                               gACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCa          >  2:898183/1‑93 (MQ=255)

                                                                               gACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCa          >  2:958575/1‑93 (MQ=255)

                                                                                  accaccCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActa       >  1:3268249/1‑93 (MQ=255)

                                                                                    caccCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActacc     <  1:2481170/93‑1 (MQ=255)

                                                                                     accCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActacct    >  2:4270951/1‑93 (MQ=255)

                                                                                       ccACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActacctac  <  2:3788543/93‑1 (MQ=255)

                                                                                       ccACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActacctac  <  1:1978011/93‑1 (MQ=255)

TATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCACTACCTAC  >  NC_000913/2463920‑2464099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`