BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,470,298	A→G	I37T (ATT→ACT)	b2356 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,470,298	0	A	G	96.2%	397.8 / 0.9	132	I37T (ATT→ACT)	b2356	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (3/2); new base G (69/58); total (72/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 132 total aligned reads displayed.

GCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCC  >  NC_000913/2470208‑2470387

gcgTTGTCTTCCGCAGTGTGATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGtt                                                                                         <  1:1031574/93‑1 (MQ=255)

 cgTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTc                                                                                        >  1:3689091/1‑93 (MQ=255)

  gTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTcc                                                                                       >  1:1030365/1‑93 (MQ=255)

    tGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTcccc                                                                                     <  2:3121927/93‑1 (MQ=255)

         tCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGaaa                                                                                >  2:4515142/1‑93 (MQ=255)

           cGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAt                                                                              <  2:3896545/93‑1 (MQ=255)

            gCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtg                                                                             >  2:2566980/1‑93 (MQ=255)

            gCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtg                                                                             >  2:1623922/1‑93 (MQ=255)

              aGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgtt                                                                           >  2:348977/1‑93 (MQ=255)

               gtgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttg                                                                          <  1:4717047/93‑1 (MQ=255)

                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         >  2:1184173/1‑93 (MQ=255)

                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         >  2:1134125/1‑93 (MQ=255)

                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         >  1:4129384/1‑93 (MQ=255)

                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         <  2:1756949/93‑1 (MQ=255)

                   aaTGCGCGGCGCATTTGGCGTTATCACGATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCg                                                                      <  1:4422977/93‑1 (MQ=255)

                   aaTACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCg                                                                      <  2:4431364/93‑1 (MQ=255)

                   aaTACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCg                                                                      <  1:3956602/93‑1 (MQ=255)

                   aaTACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCg                                                                      <  2:545118/93‑1 (MQ=255)

                     tACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGc                                                                    >  1:3519033/1‑93 (MQ=255)

                     tACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGc                                                                    <  2:1060067/93‑1 (MQ=255)

                      aCGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCg                                                                   <  2:4613489/93‑1 (MQ=255)

                      aCGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCg                                                                   <  1:621284/93‑1 (MQ=255)

                       cgcgGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGt                                                                  <  2:2777748/93‑1 (MQ=255)

                        gcgGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGAGTTAGTTCCCCAGAAAATGTTGTCCGGCGTg                                                                 >  1:2728418/1‑93 (MQ=255)

                         cgGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgc                                                                <  1:3331546/93‑1 (MQ=255)

                         cgGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgc                                                                <  1:1728615/93‑1 (MQ=255)

                           gCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgcgc                                                              >  2:3935428/1‑93 (MQ=255)

                            cacaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGcc                                                             <  1:4380890/93‑1 (MQ=255)

                              caTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAc                                                           <  2:1253145/93‑1 (MQ=255)

                               aTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACt                                                          >  2:4105119/1‑93 (MQ=255)

                                tttGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTg                                                         >  1:1248909/1‑93 (MQ=255)

                                tttGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTg                                                         >  1:1052736/1‑93 (MQ=255)

                                  tGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGAt                                                       <  1:1321241/93‑1 (MQ=255)

                                  tGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGAt                                                       >  1:188887/1‑93 (MQ=255)

                                  tGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGAt                                                       <  2:4274502/93‑1 (MQ=255)

                                    gCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCg                                                     <  1:1927155/93‑1 (MQ=255)

                                    gCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCg                                                     >  2:4564992/1‑93 (MQ=255)

                                    gCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCg                                                     <  1:3473226/93‑1 (MQ=255)

                                      gTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGcc                                                   >  1:3329377/1‑93 (MQ=255)

                                      gTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGcc                                                   >  2:66530/1‑93 (MQ=255)

                                      gTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGCGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGcc                                                   >  2:3768832/1‑93 (MQ=255)

                                       ttATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGccc                                                  >  1:4242664/1‑93 (MQ=255)

                                         aTCACCATCAGTAAACAGATCCAGAGCAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACCGATCGCCCAc                                                <  2:3055512/93‑1 (MQ=255)

                                            aCCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtc                                             >  2:4104153/1‑93 (MQ=255)

                                            aCCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtc                                             >  1:3817952/1‑93 (MQ=255)

                                             ccATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcc                                            >  2:1879832/1‑93 (MQ=255)

                                             ccATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcc                                            <  1:3357697/93‑1 (MQ=255)

                                              cATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcct                                           >  2:316988/1‑93 (MQ=255)

                                              cATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcct                                           >  2:2622197/1‑93 (MQ=255)

                                               aTCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGGGGTAGTTCCCCAGAAAATGCTGTCCGGCGTGCGCCACTGATCGCCCActtcctt                                          >  1:1737675/1‑93 (MQ=255)

                                                tCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtccttc                                         <  1:3860337/93‑1 (MQ=255)

                                                 cAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCa                                        >  2:3793396/1‑93 (MQ=255)

                                                  aGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAg                                       >  2:3946934/1‑93 (MQ=255)

                                                    tAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGtt                                     >  2:445035/1‑93 (MQ=255)

                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  <  1:1563197/93‑1 (MQ=255)

                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  <  2:3194015/93‑1 (MQ=255)

                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  <  1:1731491/93‑1 (MQ=255)

                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  >  2:2225221/1‑93 (MQ=255)

                                                        cAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGCCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATg                                 >  1:699912/1‑93 (MQ=255)

                                                        cAGATCCAGAACAAAAGGGCCAAACAGGGTCTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATg                                 >  2:4574985/1‑93 (MQ=255)

                                                         aGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATgg                                <  2:2471499/93‑1 (MQ=255)

                                                          gATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATggg                               >  1:2647770/1‑93 (MQ=255)

                                                          gATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATggg                               >  1:2661416/1‑93 (MQ=255)

                                                            tCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGcg                             >  1:1623864/1‑92 (MQ=255)

                                                            tCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCt                             >  2:1297344/1‑93 (MQ=255)

                                                             ccAGAACAAACGGGCCAAACATAGTGTTAATTCCCCAGAAAACGTCGTCCTGCACACGCCGCTGATAGTCCACTTCCTGCAGTTCATGCGCTg                            >  2:1404813/1‑93 (MQ=255)

                                                             ccAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTg                            <  1:3332916/93‑1 (MQ=255)

                                                             ccAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGCTCATGGGCTg                            >  1:2697054/1‑93 (MQ=255)

                                                              cAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTgg                           >  1:366986/1‑93 (MQ=255)

                                                                gAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGtt                         >  1:3464647/1‑93 (MQ=255)

                                                                gAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGATGGtt                         >  2:3867378/1‑93 (MQ=255)

                                                                  aCAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTg                       >  2:2365413/1‑93 (MQ=255)

                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      >  1:3198293/1‑93 (MQ=255)

                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      <  2:1996208/93‑1 (MQ=255)

                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      <  1:3490912/93‑1 (MQ=255)

                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      >  2:3428932/1‑93 (MQ=255)

                                                                     aaCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTc                    <  2:506066/93‑1 (MQ=255)

                                                                     aaCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTc                    <  2:188887/93‑1 (MQ=255)

                                                                      aCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTcc                   <  2:930971/93‑1 (MQ=255)

                                                                      aCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTcc                   <  2:1780393/93‑1 (MQ=255)

                                                                      aCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTcc                   >  2:3045715/1‑93 (MQ=255)

                                                                        gggCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGc                 >  1:703401/1‑93 (MQ=255)

                                                                         ggCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCa                <  1:4171820/93‑1 (MQ=255)

                                                                            cAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCCGCt             >  2:2760181/1‑93 (MQ=255)

                                                                             aaaCAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTc            >  1:2941021/1‑93 (MQ=255)

                                                                             aaaCAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTc            >  1:3090684/1‑93 (MQ=255)

                                                                              aaCAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTcc           >  2:1429867/1‑93 (MQ=255)

                                                                               aCAGGGGGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGATGGTTTGTTTCGCAGCTccc          >  2:485298/1‑92 (MQ=255)

                                                                                cAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGATTGTTCCGCAGCTccac         >  1:3965259/1‑93 (MQ=255)

                                                                                 aGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacc        <  2:132557/93‑1 (MQ=255)

                                                                                 aGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacc        <  2:2264585/93‑1 (MQ=255)

                                                                                 aGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacc        <  2:2671040/93‑1 (MQ=255)

                                                                                  gggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacca       >  1:2794665/1‑93 (MQ=255)

                                                                                   ggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAg      >  1:3783531/1‑93 (MQ=255)

                                                                                     tgtTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAgcg    <  2:551657/93‑1 (MQ=255)

                                                                                      gtTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAgcgc   >  1:1375349/1‑93 (MQ=255)

                                                                                       ttAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGcc  <  1:4682446/93‑1 (MQ=255)

                                                                                       ttAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGcc  <  2:1231036/93‑1 (MQ=255)

                                                                                       ttAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGcc  <  1:2047020/93‑1 (MQ=255)

                                                                                       ttAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGcc  >  2:1423926/1‑93 (MQ=255)

GCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCC  >  NC_000913/2470208‑2470387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`