Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,519,341	A→G	noncoding (69/76 nt)	lysV →	tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,519,341	0	A	G	99.0%	238.0 / ‑5.1	96	noncoding (69/76 nt)	lysV	tRNA‑Lys
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (49/46);  total (50/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGC  >  NC_000913/2519251‑2519429                                                                                           |                                                                                         tCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGcc                                                                                        >  1:3780090/1‑93 (MQ=37) tCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGcc                                                                                        >  2:1425081/1‑93 (MQ=37)   ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCcca                                                                                      >  2:57790/1‑93 (MQ=37)   ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCcca                                                                                      >  1:2923802/1‑93 (MQ=37)   ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCcca                                                                                      >  2:3406027/1‑93 (MQ=37)     gTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCccacc                                                                                    >  1:1947700/1‑93 (MQ=37)     gTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTCCACGGCccacc                                                                                    >  1:2314333/1‑93 (MQ=255)       catcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCaa                                                                                  >  1:4120872/1‑93 (MQ=255)        atcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAAt                                                                                 <  2:2992175/93‑1 (MQ=255)           acccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTa                                                                              >  1:1277940/1‑93 (MQ=255)            cccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaa                                                                             <  1:4237787/93‑1 (MQ=255)             ccaccaCTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaa                                                                            >  2:1507675/1‑93 (MQ=255)             ccaccaCTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaa                                                                            <  2:3075594/93‑1 (MQ=255)             ccaccaCTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaa                                                                            >  2:4351680/1‑93 (MQ=255)              caccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaaa                                                                           <  2:3568446/93‑1 (MQ=255)              caccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaaa                                                                           >  2:4048061/1‑93 (MQ=255)              caccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaaa                                                                           >  2:4048062/1‑93 (MQ=255)              caccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaaa                                                                           >  1:1322208/1‑93 (MQ=255)               accacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCAGGGCCCACCAATGTaaaaa                                                                          >  2:4152437/1‑93 (MQ=255)                ccacTTCGGTTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaaaaa                                                                         <  2:3415702/93‑1 (MQ=255)                ccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaaaaa                                                                         <  2:2715811/93‑1 (MQ=255)                ccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTaaaaaa                                                                         >  2:31566/1‑93 (MQ=255)                 cacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAg                                                                        <  1:1104320/93‑1 (MQ=37)                  ccTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAgc                                                                       <  2:1426387/92‑1 (MQ=19)                  ccTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAgc                                                                       <  2:588046/92‑1 (MQ=19)                   cTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAgcg                                                                      >  1:491274/1‑93 (MQ=34)                    ttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAgcgc                                                                     >  2:1656687/1‑93 (MQ=18)                    ttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAgcgc                                                                     >  1:4101748/1‑93 (MQ=18)                     tCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGcc                                                                    <  2:493325/93‑1 (MQ=17)                     tCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGcc                                                                    <  2:4275791/93‑1 (MQ=17)                                      ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTa                                                   <  1:1782386/93‑1 (MQ=14)                                        ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACt                                                 <  2:1497471/93‑1 (MQ=18)                                          tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTAt                                               >  2:2040480/1‑93 (MQ=18)                                          tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTAt                                               >  2:786057/1‑93 (MQ=18)                                           agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATc                                              <  2:4425008/93‑1 (MQ=18)                                            gagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCt                                             >  1:3156294/1‑93 (MQ=18)                                            gagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCt                                             <  1:3967935/93‑1 (MQ=21)                                              gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGc                                           >  2:3995017/1‑93 (MQ=18)                                               cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCg                                          >  1:1420174/1‑93 (MQ=18)                                               cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCg                                          >  2:593949/1‑93 (MQ=18)                                                aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGa                                         >  1:3526060/1‑93 (MQ=18)                                                aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGa                                         >  1:1388489/1‑93 (MQ=18)                                                aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGa                                         >  1:908713/1‑93 (MQ=18)                                                aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGa                                         <  2:313679/93‑1 (MQ=21)                                                aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGa                                         >  1:279655/1‑93 (MQ=18)                                                 gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGAt                                        >  1:3199167/1‑93 (MQ=18)                                                  ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATa                                       >  1:4125561/1‑93 (MQ=18)                                                  ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATa                                       >  1:1603847/1‑93 (MQ=18)                                                  ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATa                                       <  1:821956/93‑1 (MQ=18)                                                   tGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAc                                      >  1:3292572/1‑93 (MQ=18)                                                   tGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAc                                      >  2:697406/1‑93 (MQ=18)                                                   tGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAc                                      <  1:2956663/93‑1 (MQ=18)                                                    gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACt                                     <  1:3288361/93‑1 (MQ=18)                                                    gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACt                                     >  2:2565014/1‑93 (MQ=18)                                                    gACTTTTAAACAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACt                                     >  1:2600601/1‑93 (MQ=255)                                                     aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTc                                    >  2:191407/1‑93 (MQ=18)                                                       ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaa                                  >  2:1671532/1‑93 (MQ=18)                                                       ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaa                                  <  2:712314/93‑1 (MQ=21)                                                        tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaaa                                 <  2:558339/93‑1 (MQ=21)                                                        tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaaa                                 <  2:2926767/93‑1 (MQ=21)                                                         ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAg                                >  2:3126814/1‑93 (MQ=18)                                                          tAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGa                               <  2:2327630/93‑1 (MQ=18)                                                          tAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGa                               <  1:4737899/93‑1 (MQ=21)                                                          tAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGa                               <  1:610726/93‑1 (MQ=18)                                                          tAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGa                               <  1:2775043/93‑1 (MQ=21)                                                           aaTCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAATAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAt                              >  2:4245178/1‑93 (MQ=16)                                                            aTCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAtt                             <  2:3790581/93‑1 (MQ=21)                                                               aaTTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGa                          <  2:1202963/93‑1 (MQ=21)                                                               aaTTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGa                          <  1:1795425/93‑1 (MQ=18)                                                                 ttGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAc                        >  1:4052628/1‑93 (MQ=18)                                                                 ttGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAc                        >  1:4052627/1‑93 (MQ=18)                                                                  tGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAcc                       >  1:4176655/1‑93 (MQ=18)                                                                    gTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTg                     <  1:202826/93‑1 (MQ=18)                                                                    gTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTg                     >  2:220018/1‑93 (MQ=18)                                                                     tCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgc                    <  2:4106339/93‑1 (MQ=18)                                                                       gCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgcag                  <  1:3987779/93‑1 (MQ=18)                                                                       gCAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgcag                  >  1:956780/1‑93 (MQ=18)                                                                        cAGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgcagc                 >  2:1406315/1‑93 (MQ=18)                                                                         aGGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgcagca                >  2:2496801/1‑93 (MQ=18)                                                                          ggTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGcagcag               <  1:3492428/93‑1 (MQ=21)                                                                          ggTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGcagcag               <  2:2572407/93‑1 (MQ=21)                                                                          ggTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGcagcag               <  2:2138491/93‑1 (MQ=18)                                                                            ttCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt             >  1:3283840/1‑93 (MQ=18)                                                                            ttCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt             <  2:2113805/93‑1 (MQ=18)                                                                            ttCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt             <  2:2006057/93‑1 (MQ=18)                                                                             tCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGtt            <  1:3816987/93‑1 (MQ=18)                                                                             tCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGtt            <  2:1290292/93‑1 (MQ=18)                                                                              cGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt           <  2:1724005/93‑1 (MQ=18)                                                                                aaTCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttga         >  1:1294385/1‑93 (MQ=18)                                                                                 aTCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgag        >  2:1205429/1‑93 (MQ=18)                                                                                  tCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagt       >  2:1203786/1‑93 (MQ=18)                                                                                    cTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttg     <  2:806163/93‑1 (MQ=18)                                                                                    cTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttg     >  2:428465/1‑93 (MQ=18)                                                                                     tGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga    >  2:4576859/1‑93 (MQ=18)                                                                                     tGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga    <  2:3984166/93‑1 (MQ=18)                                                                                     tGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga    <  2:3984165/93‑1 (MQ=21)                                                                                     tGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga    <  1:1075953/93‑1 (MQ=18)                                                                                     tGCACGCCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga    <  2:4536716/93‑1 (MQ=18)                                                                                      gCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttgag   <  1:1892417/93‑1 (MQ=18)                                                                                       cACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAgc  <  1:3570170/93‑1 (MQ=21)                                                                                           |                                                                                         TCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGC  >  NC_000913/2519251‑2519429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑