BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,532,993	A→G	D303G (GAC→GGC)	ptsI →	PEP‑protein phosphotransferase system enzyme I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,532,993	0	A	G	100.0%	459.2 / NA	142	D303G (GAC→GGC)	ptsI	PEP‑protein phosphotransferase system enzyme I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (56/86); total (56/86)

Only 100 of 142 total aligned reads displayed.

CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082

 tAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcg                                                                                        <  1:4525728/93‑1 (MQ=255)

   aCATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcgcg                                                                                      <  2:1050014/93‑1 (MQ=255)

          tACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACt                                                                               <  2:895323/93‑1 (MQ=255)

            cGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGc                                                                             >  2:1178580/1‑93 (MQ=255)

            cGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGc                                                                             <  1:48822/93‑1 (MQ=255)

              gTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           <  1:1093249/93‑1 (MQ=255)

              gTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           >  2:791066/1‑93 (MQ=255)

              gTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           >  2:2387265/1‑93 (MQ=255)

                  gTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTg                                                                       >  2:2128875/1‑93 (MQ=255)

                   tGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTga                                                                      <  1:2100811/93‑1 (MQ=255)

                    gACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaa                                                                     <  1:268652/93‑1 (MQ=255)

                      cGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaaga                                                                   <  1:563606/93‑1 (MQ=255)

                      cGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaaga                                                                   <  1:2035104/93‑1 (MQ=255)

                         tGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaaga                                                                <  1:2142897/93‑1 (MQ=255)

                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               <  2:897184/93‑1 (MQ=255)

                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               >  1:3915693/1‑93 (MQ=255)

                           aaGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAc                                                              <  2:3148201/93‑1 (MQ=255)

                           aaGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAc                                                              <  2:3148202/93‑1 (MQ=255)

                           aaGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAc                                                              <  2:1006828/93‑1 (MQ=255)

                            aGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACa                                                             <  2:1893664/93‑1 (MQ=255)

                            aGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACa                                                             >  2:4625570/1‑93 (MQ=255)

                            aGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACa                                                             <  1:3008827/93‑1 (MQ=255)

                             ggTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAg                                                            <  1:2244350/93‑1 (MQ=255)

                             ggTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAg                                                            >  2:2068318/1‑93 (MQ=255)

                             ggTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAg                                                            <  2:2732963/93‑1 (MQ=255)

                               tGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGtt                                                          <  2:1314610/93‑1 (MQ=255)

                               ggCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCTGCGACGCACTGCCCACTGAAGAAGAACAGtt                                                          <  1:3456595/92‑1 (MQ=255)

                                  agagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgc                                                       <  2:2865431/93‑1 (MQ=255)

                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      >  1:4581248/1‑93 (MQ=255)

                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      <  1:556427/93‑1 (MQ=255)

                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      <  1:3789062/93‑1 (MQ=255)

                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      <  2:2263078/93‑1 (MQ=255)

                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      >  2:207730/1‑93 (MQ=255)

                                      cGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTgctgct                                                   <  2:702480/93‑1 (MQ=255)

                                       gTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCtt                                                  <  1:3401792/93‑1 (MQ=255)

                                       gTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCtt                                                  <  2:4405204/93‑1 (MQ=255)

                                       gTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCtt                                                  <  1:2181581/93‑1 (MQ=255)

                                        tAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTa                                                 >  2:381981/1‑93 (MQ=255)

                                           cGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACaa                                              >  1:779484/1‑93 (MQ=255)

                                           cGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACaa                                              >  1:2535258/1‑93 (MQ=255)

                                             gcgcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAg                                            <  2:665163/93‑1 (MQ=255)

                                               gcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGGACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCa                                          >  1:75323/1‑93 (MQ=255)

                                               gcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCa                                          <  2:2997411/93‑1 (MQ=255)

                                               gcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCa                                          <  1:1570507/93‑1 (MQ=255)

                                               gcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCa                                          >  2:3411203/1‑93 (MQ=255)

                                               gcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCa                                          <  1:2526232/93‑1 (MQ=255)

                                                cTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAg                                         <  2:667220/93‑1 (MQ=255)

                                                   aaGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACaaa                                             <  1:2241566/86‑1 (MQ=255)

                                                   aaGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACaaa                                             >  2:2241566/1‑86 (MQ=255)

                                                   aaGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTgg                                      >  2:3077928/1‑93 (MQ=255)

                                                   aaGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTgg                                      <  2:3934516/93‑1 (MQ=255)

                                                   aaGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCCGCCTACAAAGCAGTgg                                      >  1:4197457/1‑93 (MQ=255)

                                                     ggCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCt                                    <  2:3102450/93‑1 (MQ=255)

                                                      gCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTg                                   >  2:2309358/1‑93 (MQ=255)

                                                        gTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 >  1:2796387/1‑93 (MQ=255)

                                                        gTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 >  2:2003400/1‑93 (MQ=255)

                                                         ttGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAg                                >  1:1238462/1‑93 (MQ=255)

                                                             tCTGTATCGTACTGAGTTCCTGTTCATGGGGCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTATAAAGCAGTCGCTGAAGCgtg                            >  2:300605/1‑93 (MQ=255)

                                                             tCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtg                            <  2:4695159/93‑1 (MQ=255)

                                                             tCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtg                            >  1:3578769/1‑93 (MQ=255)

                                                              cTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtgt                           >  1:3329143/1‑93 (MQ=255)

                                                                gTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTgg                         <  1:344940/93‑1 (MQ=255)

                                                                gTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTgg                         <  1:344941/93‑1 (MQ=255)

                                                                 tATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGc                        <  1:1029048/93‑1 (MQ=255)

                                                                  aTCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCt                       >  1:1033085/1‑93 (MQ=255)

                                                                  aTCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCt                       <  1:3767503/93‑1 (MQ=255)

                                                                  aTCGTACTGAGTGCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCt                       <  1:2823289/93‑1 (MQ=255)

                                                                   tCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTc                      <  2:1523487/93‑1 (MQ=255)

                                                                    cGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCg                     <  2:4474243/93‑1 (MQ=255)

                                                                     gTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACCAAGCCGTGGCTGCGGCGTGTGGCTCGc                    >  1:3552870/1‑93 (MQ=255)

                                                                     gTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTCCTTACAAAGCAGTGCCTGAAGCGTGTGGCTCGc                    >  1:2344291/1‑93 (MQ=255)

                                                                      tACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCa                   >  1:1567852/1‑93 (MQ=255)

                                                                       aCTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCaa                  <  1:3067637/93‑1 (MQ=255)

                                                                       aCTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCaa                  <  1:3800999/93‑1 (MQ=255)

                                                                        cTGAGTTCCTGTTTATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 <  2:1326713/93‑1 (MQ=255)

                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 <  1:1948787/93‑1 (MQ=255)

                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 <  1:578669/93‑1 (MQ=255)

                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 <  1:4616489/93‑1 (MQ=255)

                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 <  1:1426774/93‑1 (MQ=255)

                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                <  1:969608/93‑1 (MQ=255)

                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                >  1:4322093/1‑93 (MQ=255)

                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                <  2:3537514/93‑1 (MQ=255)

                                                                           aGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCgg              >  1:1304136/1‑93 (MQ=255)

                                                                            gTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGt             <  1:383949/93‑1 (MQ=255)

                                                                            gTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGt             >  2:3555687/1‑93 (MQ=255)

                                                                             ttCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGtt            >  2:1602285/1‑93 (MQ=255)

                                                                             ttCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGtt            <  1:1863420/93‑1 (MQ=255)

                                                                             ttCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGtt            <  1:1863419/93‑1 (MQ=255)

                                                                              tCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTa           <  2:2923575/93‑1 (MQ=255)

                                                                               ccTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAt          <  1:4139673/93‑1 (MQ=255)

                                                                               ccTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAt          <  1:29878/93‑1 (MQ=255)

                                                                                cTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtc         >  1:4492057/1‑93 (MQ=255)

                                                                                 tGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcg        >  1:2752221/1‑93 (MQ=255)

                                                                                  gTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgt       <  2:3761177/93‑1 (MQ=255)

                                                                                  gTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgt       >  1:670101/1‑93 (MQ=255)

                                                                                   ttCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgtt      <  1:1393276/93‑1 (MQ=255)

                                                                                     cATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttcg    <  2:4576096/93‑1 (MQ=255)

                                                                                      aTGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttcgt   >  2:2502354/1‑93 (MQ=255)

                                                                                       tGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTa  >  2:2899274/1‑93 (MQ=255)

                                                                                       tGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTa  >  1:128477/1‑93 (MQ=255)

CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`