Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,557,200 T→C V137A (GTG→GCG)  b2442 → putative prophage integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,557,2000TC99.2% 370.0 / ‑4.6 117V137A (GTG→GCG) b2442putative prophage integrase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (58/58);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

Only 100 of 123 total aligned reads displayed.

CCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATC  >  NC_000913/2557110‑2557289
                                                                                          |                                                                                         
cccTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCgg                                                                                         >  2:156964/1‑93 (MQ=255)
cccTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCgg                                                                                         <  2:1838096/93‑1 (MQ=255)
  cTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGgcggcg                                                                                       <  1:1018130/93‑1 (MQ=255)
  cTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGgcggcg                                                                                       <  2:385224/93‑1 (MQ=255)
  cTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGgcggcg                                                                                       <  1:1018131/93‑1 (MQ=255)
  cTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCg                                                                                       >  1:88555/1‑93 (MQ=255)
   ttATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGcggcgg                                                                                      >  1:1190702/1‑93 (MQ=255)
        tccAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCTGCAGGATTTAAGAAGGCGGCGGAAGAc                                                                                 <  2:3462730/92‑1 (MQ=255)
        gCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGAc                                                                                 <  2:3346389/93‑1 (MQ=255)
        gCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGAc                                                                                 <  2:2524458/93‑1 (MQ=255)
          cAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTg                                                                               <  1:3402455/93‑1 (MQ=255)
          cAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTg                                                                               <  2:1087585/93‑1 (MQ=255)
           aaTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTgg                                                                              >  1:2982934/1‑93 (MQ=255)
           aaTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTgg                                                                              >  1:1539405/1‑93 (MQ=255)
            aTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGt                                                                             <  1:2499966/93‑1 (MQ=255)
             tGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGtt                                                                            >  1:200549/1‑93 (MQ=255)
              ggAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGttt                                                                           <  1:3705547/93‑1 (MQ=255)
              ggAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGttt                                                                           <  1:2994972/93‑1 (MQ=255)
                  ttAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTa                                                                       >  1:4626480/1‑93 (MQ=255)
                   tAACCCAGTGCACCAAGCCAGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTAc                                                                      <  2:33184/93‑1 (MQ=255)
                     aCCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCa                                                                    >  1:854234/1‑93 (MQ=255)
                     aCCCAGTGGAACAAGCCCGCAACGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCa                                                                    >  2:965389/1‑93 (MQ=255)
                      cccAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAg                                                                   >  1:2732414/1‑93 (MQ=255)
                      cccAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAg                                                                   >  2:1805716/1‑93 (MQ=255)
                       ccAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAgg                                                                  <  2:1571547/93‑1 (MQ=255)
                       ccAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAgg                                                                  >  1:4198983/1‑93 (MQ=255)
                         aGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGt                                                                >  2:806051/1‑93 (MQ=255)
                          gTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGtt                                                               <  1:1005094/93‑1 (MQ=255)
                          gTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGtt                                                               <  2:3591886/93‑1 (MQ=255)
                           tGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTg                                                              >  2:1027673/1‑93 (MQ=255)
                           tGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTg                                                              >  2:3586424/1‑93 (MQ=255)
                             gAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGa                                                            <  2:1492518/93‑1 (MQ=255)
                               aCAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAgg                                                          <  2:4661814/93‑1 (MQ=255)
                               aCAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGGTGGAgg                                                          >  1:256315/1‑93 (MQ=255)
                                 aaGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggt                                                        <  2:930788/93‑1 (MQ=255)
                                 aaGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggt                                                        <  2:910880/93‑1 (MQ=255)
                                 aaGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGAATGGTTTGCTACCAGGGTTGGAgggg                                                        >  2:1228766/1‑92 (MQ=255)
                                  aGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtg                                                       >  1:4188768/1‑93 (MQ=255)
                                  aGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtg                                                       <  1:2833668/93‑1 (MQ=255)
                                   gCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                                                      >  1:2738874/1‑93 (MQ=255)
                                    cccGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtggt                                                     <  1:431228/93‑1 (MQ=255)
                                    cccGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtggt                                                     <  1:483962/93‑1 (MQ=255)
                                      cGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCg                                                   >  1:1519535/1‑93 (MQ=255)
                                       gCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCgg                                                  >  1:2858759/1‑93 (MQ=255)
                                       gCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCgg                                                  <  1:1109134/93‑1 (MQ=255)
                                         aaGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGaa                                                >  1:4279529/1‑93 (MQ=255)
                                              aaaaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTa                                           <  1:4558712/93‑1 (MQ=255)
                                               aaaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTAt                                          <  2:1644710/93‑1 (MQ=255)
                                               aaaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTAt                                          <  2:1644711/93‑1 (MQ=255)
                                               aaaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTAt                                          <  1:699555/93‑1 (MQ=255)
                                               aaaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTAt                                          >  2:4059883/1‑93 (MQ=255)
                                                aaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATg                                         >  1:1791591/1‑93 (MQ=255)
                                                aaGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATg                                         >  2:488871/1‑93 (MQ=255)
                                                  gCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCa                                       >  1:3586284/1‑93 (MQ=255)
                                                   ccATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCaa                                      >  2:1726379/1‑93 (MQ=255)
                                                   ccATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCaa                                      <  2:2774827/93‑1 (MQ=255)
                                                     aTTGATGCGCTCAACATGGCGGCAGGATTTTAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGACGGTGGTCGGAATCCTATGCaaaa                                    >  1:4655074/1‑93 (MQ=255)
                                                     aTTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGGTGGAGGGTGGTCGGAATCCTATGCaaaa                                    >  1:2617564/1‑93 (MQ=255)
                                                      ttGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAc                                   <  2:1383124/93‑1 (MQ=255)
                                                      ttGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAc                                   >  1:4246493/1‑93 (MQ=255)
                                                         aTGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAgg                                <  2:2080784/93‑1 (MQ=255)
                                                         aTGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAgg                                >  2:2141779/1‑93 (MQ=255)
                                                          tGCGCTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGt                               <  2:1852001/93‑1 (MQ=255)
                                                           gcgcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTc                              <  1:913457/93‑1 (MQ=255)
                                                           gcgcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTc                              <  2:2954550/93‑1 (MQ=255)
                                                            cgcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCa                             <  2:1829565/93‑1 (MQ=255)
                                                            cgcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCa                             <  1:2353213/93‑1 (MQ=255)
                                                             gcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAg                            <  1:1425472/93‑1 (MQ=255)
                                                             gcTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAg                            <  2:2821949/93‑1 (MQ=255)
                                                              cTCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGa                           <  1:3878500/93‑1 (MQ=255)
                                                               tCAACATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGAt                          >  1:2399983/1‑93 (MQ=255)
                                                                  aCATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCgg                       >  1:496317/1‑93 (MQ=255)
                                                                  aCATGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCgg                       <  1:1224683/93‑1 (MQ=255)
                                                                     tGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCAc                    >  2:3288515/1‑93 (MQ=255)
                                                                     tGGCGGCAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCAc                    >  2:4601631/1‑93 (MQ=255)
                                                                        cggcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAg                 >  2:2845857/1‑93 (MQ=255)
                                                                        cggcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAg                 <  2:2999399/93‑1 (MQ=255)
                                                                        cggcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAg                 >  1:2595837/1‑93 (MQ=255)
                                                                        cggcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAg                 >  2:3996905/1‑93 (MQ=255)
                                                                         ggcAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGa                >  2:3621757/1‑93 (MQ=255)
                                                                           cAGGATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaaa              <  2:2456269/93‑1 (MQ=255)
                                                                             ggATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaaaaa            <  1:669374/93‑1 (MQ=255)
                                                                             ggATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaaaaa            >  1:2290598/1‑93 (MQ=255)
                                                                             ggATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGaaaaa            <  2:471903/93‑1 (MQ=255)
                                                                              gATTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAg           >  2:1203644/1‑93 (MQ=255)
                                                                               aTTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGa          >  2:2939937/1‑93 (MQ=255)
                                                                               aTTTAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGa          <  1:4506098/93‑1 (MQ=255)
                                                                                tttAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGAc         >  1:2269012/1‑93 (MQ=255)
                                                                                tttAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGAc         >  1:2128948/1‑93 (MQ=255)
                                                                                tttAAGAAGGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGAc         >  1:2269011/1‑93 (MQ=255)
                                                                                  tAAGAAGGCGGCGGAAGACTGGTGTGCTCACAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGt       <  1:3175861/93‑1 (MQ=255)
                                                                                   aagaagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTc      >  2:4095794/1‑93 (MQ=255)
                                                                                   aagaagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTc      <  1:124709/93‑1 (MQ=255)
                                                                                   aagaagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTc      <  2:2802388/93‑1 (MQ=255)
                                                                                    agaagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCt     >  2:2373088/1‑93 (MQ=255)
                                                                                     gaagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTa    >  2:3727867/1‑93 (MQ=255)
                                                                                     gaagGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTa    <  1:3179411/93‑1 (MQ=255)
                                                                                       agGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATc  <  2:2301419/93‑1 (MQ=255)
                                                                                       agGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATc  <  2:4077989/93‑1 (MQ=255)
                                                                                       agGCGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATc  >  1:1069850/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGCACTAGAAAAAGACGTCTATC  >  NC_000913/2557110‑2557289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑