BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,560,887	T→C	V115A (GTG→GCG)	b2447 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,560,887	0	T	C	98.7%	463.1 / ‑3.5	149	V115A (GTG→GCG)	b2447	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (77/70); total (78/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

Only 100 of 157 total aligned reads displayed.

ATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGC  >  NC_000913/2560797‑2560976

aTCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGgcgc                                                                                         >  1:1682553/1‑93 (MQ=255)

aTCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGTGc                                                                                         >  1:4279651/1‑93 (MQ=255)

 tCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCt                                                                                        >  1:3472699/1‑93 (MQ=255)

  ccAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCtt                                                                                       <  1:1697245/93‑1 (MQ=255)

  ccAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCtt                                                                                       >  1:3712680/1‑93 (MQ=255)

  ccAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCtt                                                                                       >  1:4334854/1‑93 (MQ=255)

  ccAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCtt                                                                                       >  2:2608885/1‑93 (MQ=255)

  ccAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCtt                                                                                       >  2:1794059/1‑93 (MQ=255)

    aGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGc                                                                                     >  1:1153527/1‑93 (MQ=255)

     gAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGcc                                                                                    >  1:2730417/1‑93 (MQ=255)

     gAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGcc                                                                                    <  1:4001872/93‑1 (MQ=255)

     gAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGcc                                                                                    >  2:4561282/1‑93 (MQ=255)

      aaTATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCt                                                                                   <  2:2442991/93‑1 (MQ=255)

       atatGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTg                                                                                  <  1:1681074/93‑1 (MQ=255)

       atatGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTg                                                                                  <  2:3719459/93‑1 (MQ=255)

        tatGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTgg                                                                                 <  1:3504093/93‑1 (MQ=255)

         atGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGTCAAGGGGGTCGGGAACAGCAAGGAGCTTGCCgggg                                                                                >  1:3202100/1‑93 (MQ=255)

         atGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTggg                                                                                >  1:3202101/1‑93 (MQ=255)

            ggAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGa                                                                             <  1:3294034/92‑1 (MQ=255)

              aaGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGc                                                                           >  2:4178698/1‑93 (MQ=255)

               aGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCt                                                                          <  1:115949/93‑1 (MQ=255)

               aGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCt                                                                          <  2:2999303/93‑1 (MQ=255)

               aGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCt                                                                          <  2:4662369/93‑1 (MQ=255)

                gCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCtt                                                                         >  1:278624/1‑93 (MQ=255)

                 cACTGGAAGATGAAAGGCGTCATGAATGACAAACCCAGCTTCCACGCCGACAAGTGGGTAAGATACAGAAAGGCGCTTGCATGGGTGAGCTTa                                                                        <  2:3875788/93‑1 (MQ=255)

                   cTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATc                                                                      <  2:579642/93‑1 (MQ=255)

                     ggAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGc                                                                    >  1:4185367/1‑93 (MQ=255)

                     ggAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGc                                                                    >  1:4185368/1‑93 (MQ=255)

                      gAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGca                                                                   >  1:941496/1‑93 (MQ=255)

                       aaCATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGcac                                                                  <  1:2624273/93‑1 (MQ=255)

                        aCATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGcaca                                                                 <  1:3570992/93‑1 (MQ=255)

                        aCATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGcaca                                                                 >  2:666926/1‑93 (MQ=255)

                        aCATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGcaca                                                                 >  1:1622710/1‑93 (MQ=255)

                        aCATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGGACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGcaca                                                                 >  2:4182053/1‑93 (MQ=255)

                          aTGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACaaa                                                               >  1:2556945/1‑93 (MQ=255)

                          aTGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGCACAGCAAGGCGCTGGCCTGGGTGAGCTTATCGCACaaa                                                               >  2:1214630/1‑93 (MQ=255)

                           tGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAg                                                              >  2:4294634/1‑93 (MQ=255)

                             aaaGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGac                                                            <  1:1565933/93‑1 (MQ=255)

                             aaaGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGac                                                            <  2:3901151/93‑1 (MQ=255)

                              aaGGCGGCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGaca                                                           >  1:1752868/1‑93 (MQ=255)

                               aGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGacac                                                          <  1:280717/93‑1 (MQ=255)

                               aGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGacac                                                          >  1:905105/1‑93 (MQ=255)

                                ggCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACAcc                                                         >  1:2052046/1‑93 (MQ=255)

                                ggCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACAcc                                                         >  2:3205393/1‑93 (MQ=255)

                                 gCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCt                                                        <  1:1935513/93‑1 (MQ=255)

                                 gCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCt                                                        <  2:3240416/93‑1 (MQ=255)

                                   gTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTc                                                      >  2:1254210/1‑93 (MQ=255)

                                     cATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCAc                                                    >  1:2086577/1‑93 (MQ=255)

                                        gaatgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACggg                                                 <  1:4075348/93‑1 (MQ=255)

                                        gaatgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACggg                                                 >  2:498647/1‑93 (MQ=255)

                                         aatgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGt                                                >  1:1461736/1‑93 (MQ=255)

                                         aatgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGt                                                <  2:1886524/93‑1 (MQ=255)

                                         aatgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGt                                                <  1:1341980/93‑1 (MQ=255)

                                           tgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGc                                              >  2:2955718/1‑93 (MQ=255)

                                            gaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCg                                             <  1:1121626/93‑1 (MQ=255)

                                            gaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCg                                             <  2:1150945/93‑1 (MQ=255)

                                               aaaaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGaaa                                          <  2:2334204/93‑1 (MQ=255)

                                               aaaaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGaaa                                          >  2:2785676/1‑93 (MQ=255)

                                                aaaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAAc                                         >  1:4724003/1‑93 (MQ=255)

                                                 agcccGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACg                                        <  2:1607704/91‑1 (MQ=255)

                                                 aaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTCCGAAACg                                        >  1:1380763/1‑93 (MQ=255)

                                                  aCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACgg                                       <  2:4262809/93‑1 (MQ=255)

                                                  aCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACgg                                       <  2:4183858/93‑1 (MQ=255)

                                                    ccGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGtt                                     <  2:4383553/93‑1 (MQ=255)

                                                      gCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTc                                   >  1:702277/1‑93 (MQ=255)

                                                       cTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCa                                  >  2:2893248/1‑93 (MQ=255)

                                                        ttCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCaa                                 >  2:561555/1‑93 (MQ=255)

                                                         tCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAAt                                >  2:4033269/1‑93 (MQ=255)

                                                            ccGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCTTGGGTGAGCTTATCTCACAAAGCCGCCTTCACGGGTGCGAAACGGTTTCAATACt                             <  2:3680045/92‑1 (MQ=255)

                                                             cGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACtt                            <  2:2534377/93‑1 (MQ=255)

                                                               ccGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTcc                          >  2:3282072/1‑93 (MQ=255)

                                                                 gACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGGTTCAATACTTGCat                        >  1:4370072/1‑92 (MQ=255)

                                                                  aCAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCaaa                       <  1:3354587/93‑1 (MQ=255)

                                                                   gaaGTGGGTAGGGTACAGCAAGGCGCTTGCGTGGGTGAGCTTATCGCACAAAGACTCCTTCACGGTTGCGAAACGGTTTCAATACTTCCAAAc                      <  2:520/92‑1 (MQ=255)

                                                                   cAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAAc                      <  1:2973137/93‑1 (MQ=255)

                                                                     aGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGa                    >  1:4474550/1‑93 (MQ=255)

                                                                      gTGGTTAGGGTACAGCAAGGCGCTTGCCGGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGAt                   <  1:2582711/93‑1 (MQ=255)

                                                                        gggtagggtaCAGCAAGGCGCTTCCCTGGGTCGGCTTATCGCACAAAGACACCTTCACGGGTTCGAAACGGTTTCAATGCTTCCAAACGATGt                 <  1:1002288/93‑1 (MQ=255)

                                                                         ggtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTa                <  1:3786001/93‑1 (MQ=255)

                                                                          gtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTAc               <  2:2085221/93‑1 (MQ=255)

                                                                              ggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACa           <  2:2329670/93‑1 (MQ=255)

                                                                              ggtaCAGCAAGGCGCTTACCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGCCa           <  2:4213516/93‑1 (MQ=255)

                                                                                taCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATg         <  1:536728/93‑1 (MQ=255)

                                                                                taCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATg         <  2:1568948/93‑1 (MQ=255)

                                                                                taCAGCAAGGCGCTAGCCTGGTTGAGCTTAACGCACAAAGACCCCTTCACGGGTGCGAAACGGTTTCAATACTGCCAAACGATGTACGACATg         <  1:547683/93‑1 (MQ=255)

                                                                                taCAGCAAGGCGCGTGCCTGTGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATg         <  1:4302928/93‑1 (MQ=255)

                                                                                 aCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATgg        >  1:3483434/1‑93 (MQ=255)

                                                                                 aCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATgg        >  2:3947960/1‑93 (MQ=255)

                                                                                 aCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATgg        >  2:3277857/1‑93 (MQ=255)

                                                                                  cAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGa       >  2:1051686/1‑93 (MQ=255)

                                                                                  cAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGa       <  1:1294115/93‑1 (MQ=255)

                                                                                  cAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGa       >  2:1357976/1‑93 (MQ=255)

                                                                                  cAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGa       >  1:1537411/1‑93 (MQ=255)

                                                                                    gCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAAc     <  1:4581739/93‑1 (MQ=255)

                                                                                     cAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACg    >  2:4290663/1‑93 (MQ=255)

                                                                                      aaGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACgg   >  2:380685/1‑93 (MQ=255)

                                                                                       aGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGc  >  1:231584/1‑93 (MQ=255)

                                                                                       aGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGc  >  2:1500553/1‑93 (MQ=255)

                                                                                       aGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGc  <  2:2252939/93‑1 (MQ=255)

                                                                                       aGGCGCTTGCCTGGGGGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGc  >  1:231583/1‑93 (MQ=255)

ATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGC  >  NC_000913/2560797‑2560976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`