BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,645,360	T→C	I1650V (ATT→GTT)	b2520 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,645,360	0	T	C	97.9%	436.7 / ‑1.6	140	I1650V (ATT→GTT)	b2520	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (72/65); total (74/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 144 total aligned reads displayed.

ACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGG  >  NC_000913/2645270‑2645447

aCGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACca                                                                                       >  1:4573360/1‑93 (MQ=255)

aCGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACca                                                                                       >  2:1097954/1‑93 (MQ=255)

aCGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACca                                                                                       <  2:702878/93‑1 (MQ=255)

 cGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGGTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcag                                                                                      >  1:1948333/1‑93 (MQ=255)

   cTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagca                                                                                    >  1:661194/1‑93 (MQ=255)

    tGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagcag                                                                                   <  1:3604043/93‑1 (MQ=255)

    tGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagcag                                                                                   >  1:3356394/1‑93 (MQ=255)

    tGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagcag                                                                                   >  2:2675678/1‑93 (MQ=255)

     gCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGa                                                                                  <  1:91204/93‑1 (MQ=255)

      cAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGAt                                                                                 >  2:3989856/1‑93 (MQ=255)

      cAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGAt                                                                                 >  1:1502883/1‑93 (MQ=255)

      cAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGAt                                                                                 >  1:747472/1‑93 (MQ=255)

      cAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGAt                                                                                 <  2:3117520/93‑1 (MQ=255)

       aaGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATc                                                                                <  2:1180383/93‑1 (MQ=255)

        aGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCt                                                                               <  1:1313951/93‑1 (MQ=255)

         gAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCtt                                                                              >  1:1349785/1‑93 (MQ=255)

          aaaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTc                                                                             <  1:2142170/93‑1 (MQ=255)

           aaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCTCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCa                                                                            <  2:1402847/93‑1 (MQ=255)

           aaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTcc                                                                            >  2:2653303/1‑92 (MQ=255)

           aaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCa                                                                            <  1:1981626/93‑1 (MQ=255)

           aaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCa                                                                            >  1:546770/1‑93 (MQ=255)

           aaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCa                                                                            <  2:1402714/93‑1 (MQ=255)

             aaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGc                                                                          >  2:2215541/1‑93 (MQ=255)

             aaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGc                                                                          <  1:1137214/93‑1 (MQ=255)

              aGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGcc                                                                         >  1:2390999/1‑93 (MQ=255)

                 agaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCcgcg                                                                      >  1:3255614/1‑93 (MQ=255)

                  gaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCgcgc                                                                     >  1:1951991/1‑93 (MQ=255)

                  gaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCgcgc                                                                     >  1:4089666/1‑93 (MQ=255)

                  gaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCgcgc                                                                     >  1:1094719/1‑93 (MQ=255)

                  gaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGCTAACAAGCGAGGCATTTACGGTCTGACAACCAGCACATCTTCAGCCgcgc                                                                     >  2:1945194/1‑93 (MQ=255)

                   aaaGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGcc                                                                    <  2:4438847/93‑1 (MQ=255)

                     aGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCgg                                                                  <  2:1657885/93‑1 (MQ=255)

                     aGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCgg                                                                  >  1:839203/1‑93 (MQ=255)

                      gggCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGt                                                                 >  1:4708606/1‑93 (MQ=255)

                       ggCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTc                                                                <  1:4301160/93‑1 (MQ=255)

                        gcgcGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcg                                                               >  1:1180421/1‑93 (MQ=255)

                         cgcgGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgc                                                              >  2:3615684/1‑93 (MQ=255)

                          gcgGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgcc                                                             <  2:965856/93‑1 (MQ=255)

                           cgGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgccc                                                            >  1:2582114/1‑93 (MQ=255)

                            ggCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgcccg                                                           >  1:4573017/1‑93 (MQ=255)

                            ggCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgcccg                                                           >  2:2606546/1‑93 (MQ=255)

                               tGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgcccgcct                                                        >  1:3398850/1‑92 (MQ=255)

                               tGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCa                                                        <  2:3312517/93‑1 (MQ=255)

                               tGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCa                                                        <  2:1354054/93‑1 (MQ=255)

                               tGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCa                                                        >  1:3398849/1‑93 (MQ=255)

                                 ccAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCAtt                                                      >  1:4074388/1‑93 (MQ=255)

                                   aaCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTgg                                                    <  1:2617513/93‑1 (MQ=255)

                                   aaCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTgg                                                    <  2:4502640/93‑1 (MQ=255)

                                    aCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTggg                                                   >  1:2237048/1‑93 (MQ=255)

                                    aCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTggg                                                   >  2:476343/1‑93 (MQ=255)

                                       ttATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                <  1:3636808/93‑1 (MQ=255)

                                       ttATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                >  1:2408368/1‑93 (MQ=255)

                                       ttATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                >  2:3767342/1‑93 (MQ=255)

                                       ttATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                >  2:3821917/1‑93 (MQ=255)

                                        tATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAac                                               <  2:3556849/93‑1 (MQ=255)

                                        tATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAac                                               <  2:4011703/93‑1 (MQ=255)

                                         aTCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAaca                                              >  1:3122831/1‑93 (MQ=255)

                                          tCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTCACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacat                                             >  2:201187/1‑93 (MQ=255)

                                           ccAGCAGCATGGTTTGGTAAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGACCTTCAGCCGCGCCGGTTGCCCGCCATTGGGGAacata                                            <  2:1333327/93‑1 (MQ=255)

                                             agcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacataca                                          <  1:632726/93‑1 (MQ=255)

                                              gcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacatacat                                         >  1:577661/1‑93 (MQ=255)

                                              gcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacatacat                                         >  2:504624/1‑93 (MQ=255)

                                               cagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACAtt                                        <  1:699636/93‑1 (MQ=255)

                                                agcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACAtta                                       >  1:539824/1‑92 (MQ=255)

                                                 gcCGTGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGa                                      <  2:436997/93‑1 (MQ=255)

                                                 gcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGa                                      >  2:2552047/1‑93 (MQ=255)

                                                 gcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGa                                      <  2:3243729/93‑1 (MQ=255)

                                                  ccGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAt                                     <  2:1840521/93‑1 (MQ=255)

                                                  ccGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAt                                     <  2:3601405/93‑1 (MQ=255)

                                                   cgcgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAtt                                    >  2:785320/1‑93 (MQ=255)

                                                       tttGGTTAACAAGCTAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccac                                <  2:1063297/93‑1 (MQ=255)

                                                        ttGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccacc                               <  1:1178139/93‑1 (MQ=255)

                                                         tGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccacca                              <  2:1450169/93‑1 (MQ=255)

                                                         tGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccacca                              <  2:1176082/93‑1 (MQ=255)

                                                          ggTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCAt                             <  1:1552355/93‑1 (MQ=255)

                                                            ttAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCg                           >  2:647389/1‑93 (MQ=255)

                                                              aaCAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGt                         >  1:4527724/1‑93 (MQ=255)

                                                               aCAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGtt                        >  1:4670722/1‑93 (MQ=255)

                                                                  aGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCg                     >  1:1133110/1‑93 (MQ=255)

                                                                     gAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGGTGCggca                  >  2:3015437/1‑91 (MQ=255)

                                                                     gAGGAATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGCCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGtc                  >  2:4139410/1‑92 (MQ=255)

                                                                       ggCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAcc                <  1:2376579/93‑1 (MQ=255)

                                                                       ggCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAcc                <  1:2787821/93‑1 (MQ=255)

                                                                        gCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCt               <  2:3891443/93‑1 (MQ=255)

                                                                          ctttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTGGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGa             <  2:1689895/92‑1 (MQ=255)

                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt            >  1:828410/1‑93 (MQ=255)

                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt            >  2:4545272/1‑93 (MQ=255)

                                                                             tACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATAc          >  2:1613473/1‑93 (MQ=255)

                                                                              aCGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACg         <  1:2451181/93‑1 (MQ=255)

                                                                              aCGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACg         >  2:4215773/1‑93 (MQ=255)

                                                                                ggTCTGACAACCAGCAGATCTTCAGCCGCGACGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGtt       >  2:3241155/1‑93 (MQ=255)

                                                                                 gTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTc      >  1:4374502/1‑93 (MQ=255)

                                                                                 gTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTc      >  1:4374503/1‑93 (MQ=255)

                                                                                 gTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTc      >  2:3454854/1‑93 (MQ=255)

                                                                                  tCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTcc     >  2:2321666/1‑93 (MQ=255)

                                                                                  tCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTcc     >  2:2321793/1‑93 (MQ=255)

                                                                                  ccTGATAACCAGCGCATCTTCAGCGGCTCCGGTCGCCCGCCATTGGGGAACATACATTGATGCCACCATCGGTTGCGGTACCTGATACGTTcc     <  2:435816/92‑1 (MQ=255)

                                                                                   cTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTccc    <  1:4549057/93‑1 (MQ=255)

                                                                                    tGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCg   >  1:2117025/1‑93 (MQ=255)

                                                                                     gACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCgg  >  1:2580356/1‑93 (MQ=255)

ACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGG  >  NC_000913/2645270‑2645447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`