BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,865	T→A	L135Q (CTG→CAG)	suhB →	SuhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,865	0	T	A	98.0%	530.9 / 0.6	151	L135Q (CTG→CAG)	suhB	SuhB
Reads supporting (aligned to +/- strand): ref base T (2/1); new base A (74/74); total (76/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 159 total aligned reads displayed.

TGTTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAC  >  NC_000913/2661773‑2661953

tGTTCGTATCAAAGGACGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCt                                                                                          >  1:3699964/1‑93 (MQ=255)

  ttCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGa                                                                                        <  1:3632499/93‑1 (MQ=255)

  ttCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGa                                                                                        >  1:3041927/1‑93 (MQ=255)

  ttCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGa                                                                                        >  2:2351727/1‑93 (MQ=255)

  ttCGGAGCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGa                                                                                        <  1:3134867/93‑1 (MQ=255)

   tCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGaa                                                                                       <  2:1145456/93‑1 (MQ=255)

    cGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAAc                                                                                      <  2:1013723/93‑1 (MQ=255)

     gTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACg                                                                                     >  2:4747498/1‑93 (MQ=255)

     gTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACg                                                                                     >  2:3497143/1‑93 (MQ=255)

     gTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACg                                                                                     <  1:3020860/93‑1 (MQ=255)

      tATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACgg                                                                                    >  1:120571/1‑93 (MQ=255)

      tATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACgg                                                                                    >  1:2007318/1‑93 (MQ=255)

         cAAAGGCCGCACCGAAGTTTCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTa                                                                                 <  1:542479/93‑1 (MQ=255)

          aaaGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGACCTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCCAc                                                                                >  1:1540752/1‑93 (MQ=255)

          aaaGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTAc                                                                                >  1:373768/1‑93 (MQ=255)

           aaGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTAcc                                                                               >  1:2300855/1‑93 (MQ=255)

           aaGGCCGCACCGAAGTTGCTGTGGTATAAGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGCCGCACAGCAGAACGGCTAcc                                                                               >  2:2456688/1‑93 (MQ=255)

              gCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGAc                                                                            <  2:4627946/93‑1 (MQ=255)

              gCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGAc                                                                            <  2:4085537/93‑1 (MQ=255)

                cGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTg                                                                          >  1:887802/1‑93 (MQ=255)

                 gCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgc                                                                         >  1:4311321/1‑93 (MQ=255)

                 gCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgc                                                                         >  2:2684407/1‑93 (MQ=255)

                    ccGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGcgcg                                                                      >  1:3478049/1‑93 (MQ=255)

                      gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                    <  1:2922291/93‑1 (MQ=255)

                      gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                    >  1:4661353/1‑93 (MQ=255)

                      gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                    >  2:1282040/1‑93 (MQ=255)

                      gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGCGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                    >  1:3415752/1‑93 (MQ=255)

                         gTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgcagc                                                                 >  1:4048718/1‑93 (MQ=255)

                         gTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgcagc                                                                 <  2:2665475/93‑1 (MQ=255)

                          ttGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgcagca                                                                <  2:2750484/93‑1 (MQ=255)

                              tgtgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGc                                                            >  1:609550/1‑93 (MQ=255)

                              tgtgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGc                                                            >  1:413196/1‑93 (MQ=255)

                               gtgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGcc                                                           >  2:3560271/1‑92 (MQ=255)

                               gtgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCt                                                           >  1:253375/1‑93 (MQ=255)

                               gtgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCt                                                           <  1:437632/93‑1 (MQ=255)

                               gtgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCACACAGCAGAACGGCTACCAACTGCGCGGCAGCACCGcc                                                           >  2:3727879/1‑92 (MQ=255)

                               gtgGTATACGATCATATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCt                                                           <  1:4265258/93‑1 (MQ=255)

                                tgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTc                                                          <  1:2929497/93‑1 (MQ=255)

                                    ataCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGa                                                      <  2:2661455/93‑1 (MQ=255)

                                    ataCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGa                                                      >  2:4327589/1‑93 (MQ=255)

                                    ataCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGa                                                      >  2:4327586/1‑93 (MQ=255)

                                      aCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtc                                                    <  1:285057/93‑1 (MQ=255)

                                       cGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtct                                                   <  1:1818751/93‑1 (MQ=255)

                                       cGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtct                                                   <  2:3746673/93‑1 (MQ=255)

                                          tCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGCTCTCGa                                                >  1:833840/1‑93 (MQ=255)

                                          tCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGa                                                >  1:609344/1‑93 (MQ=255)

                                           ccTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGAc                                               >  1:4470389/1‑93 (MQ=255)

                                           ccTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCCACTGCGCGGCAGCACCGCTCGCGATCTCGAc                                               >  1:1502110/1‑93 (MQ=255)

                                            cTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACg                                              <  2:3553901/93‑1 (MQ=255)

                                             tATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACgg                                             >  1:954772/1‑93 (MQ=255)

                                              aTGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGt                                            <  2:3335113/93‑1 (MQ=255)

                                              aTGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGt                                            <  1:3752820/93‑1 (MQ=255)

                                                  gTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTa                                        >  2:2882410/1‑93 (MQ=255)

                                                  gTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTa                                        >  1:1506380/1‑93 (MQ=255)

                                                   tAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCCCGCGATCTCGACGGTACTAt                                       >  2:554547/1‑93 (MQ=255)

                                                     aCGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTc                                     <  1:2819542/93‑1 (MQ=255)

                                                     aCGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTc                                     >  1:373376/1‑93 (MQ=255)

                                                      cGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCt                                    <  2:2412732/93‑1 (MQ=255)

                                                       gAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTg                                   >  2:813872/1‑93 (MQ=255)

                                                       gAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTg                                   <  2:1243575/93‑1 (MQ=255)

                                                       gAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTg                                   <  1:4247510/93‑1 (MQ=255)

                                                        aaCTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTgg                                  >  1:4498442/1‑93 (MQ=255)

                                                        aaCTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTgg                                  >  2:4031567/1‑93 (MQ=255)

                                                         aCTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTggg                                 >  1:4053963/1‑92 (MQ=255)

                                                         aCTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGc                                 <  2:3837864/93‑1 (MQ=255)

                                                           tGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCgc                               >  1:3601026/1‑92 (MQ=255)

                                                           tGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGa                               >  2:1782785/1‑93 (MQ=255)

                                                            gTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGAc                              >  2:2581411/1‑93 (MQ=255)

                                                              tCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCg                            >  1:2493583/1‑93 (MQ=255)

                                                              tCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCg                            >  2:3846330/1‑93 (MQ=255)

                                                               cACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCTACCgg                           >  2:48234/1‑93 (MQ=255)

                                                                aCCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGc                          >  1:1093720/1‑93 (MQ=255)

                                                                 ccGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCt                         >  1:330243/1‑93 (MQ=255)

                                                                 ccGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCt                         >  2:3059701/1‑93 (MQ=255)

                                                                 ccGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCt                         <  1:1493930/93‑1 (MQ=255)

                                                                   gCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTc                       >  2:4174116/1‑93 (MQ=255)

                                                                    ccACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTcc                      <  1:155512/93‑1 (MQ=255)

                                                                     cACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTccc                     <  2:1414283/93‑1 (MQ=255)

                                                                     cACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTccc                     <  1:3271091/93‑1 (MQ=255)

                                                                      aCTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCg                    >  1:1006638/1‑93 (MQ=255)

                                                                        tCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGtt                  >  1:679629/1‑93 (MQ=255)

                                                                        tCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGtt                  <  2:706262/93‑1 (MQ=255)

                                                                         cgcgGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTc                 >  2:3771937/1‑93 (MQ=255)

                                                                         cgcgGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTc                 <  2:4149007/93‑1 (MQ=255)

                                                                          gcgGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCa                <  1:473246/93‑1 (MQ=255)

                                                                             gTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAg             >  2:3185650/1‑93 (MQ=255)

                                                                             gTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAg             >  1:2522578/1‑93 (MQ=255)

                                                                              tCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGc            <  1:1272892/93‑1 (MQ=255)

                                                                              tCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGc            <  2:2489626/93‑1 (MQ=255)

                                                                                aGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaa          <  1:2680293/93‑1 (MQ=255)

                                                                                 gggCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaaa         >  1:3929731/1‑93 (MQ=255)

                                                                                  ggCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaaaa        <  2:2352989/93‑1 (MQ=255)

                                                                                  ggCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaaaa        <  1:1769157/93‑1 (MQ=255)

                                                                                  ggCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaaaa        <  2:3099817/93‑1 (MQ=255)

                                                                                   gcgcACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAAc       <  2:1101435/93‑1 (MQ=255)

                                                                                    cgcACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACa      <  2:1060304/93‑1 (MQ=255)

                                                                                     gcACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAg     <  1:3797644/93‑1 (MQ=255)

                                                                                      cacaGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGt    <  2:1490947/93‑1 (MQ=255)

                                                                                       acaGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTa   <  2:3939469/93‑1 (MQ=255)

                                                                                        cagcagAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAc  <  2:2575522/93‑1 (MQ=255)

TGTTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAC  >  NC_000913/2661773‑2661953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`