BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,696,865	T→C	P53P (CCT→CCC)	yfhH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,696,865	0	T	C	97.8%	429.1 / ‑0.8	138	P53P (CCT→CCC)	yfhH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (71/64); total (72/66)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 147 total aligned reads displayed.

AGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGG  >  NC_000913/2696775‑2696954

aGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGa                                                                                         >  1:491600/1‑93 (MQ=255)

 gATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGAt                                                                                        >  2:3800309/1‑93 (MQ=255)

 gATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGAt                                                                                        <  1:743008/93‑1 (MQ=255)

 gATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTCATGCTACAACCCGAt                                                                                        >  1:3634757/1‑93 (MQ=255)

  atatGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATa                                                                                       <  2:150593/93‑1 (MQ=255)

   tatGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATAc                                                                                      <  1:2984237/93‑1 (MQ=255)

    atGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACg                                                                                     <  1:2354679/93‑1 (MQ=255)

      gAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTTCTGCTACAACCCGATACGgc                                                                                   <  1:3689833/93‑1 (MQ=255)

        aCGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGgcgc                                                                                 <  2:1751536/93‑1 (MQ=255)

        aCGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGgcgc                                                                                 <  1:1632878/93‑1 (MQ=255)

           gCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGcc                                                                              >  2:443809/1‑93 (MQ=255)

           gCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGcc                                                                              <  1:121180/93‑1 (MQ=255)

                 ttCGTATCCGTCAGCGTTACGAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTaa                                                                        <  2:2147620/93‑1 (MQ=255)

                   cGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGc                                                                      <  1:3331354/93‑1 (MQ=255)

                     tATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGctc                                                                    >  2:2661228/1‑93 (MQ=255)

                     tATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGctc                                                                    >  1:3702218/1‑93 (MQ=255)

                       tCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCtctc                                                                  <  1:3017364/93‑1 (MQ=255)

                       tCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCtctc                                                                  >  1:3828579/1‑93 (MQ=255)

                       tCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCtctc                                                                  >  2:3582796/1‑93 (MQ=255)

                        ccGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCa                                                                 <  1:3934074/93‑1 (MQ=255)

                         cGTCAGCGTTACCACGGGATTACCCAGAGGGATAAAAAACTCCTGGAATATCTGATGCTACAAGCCGATACGGCGCGCCACTTAAGCTATCAg                                                                >  2:3549865/1‑93 (MQ=255)

                           tCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCa                                                              <  1:2187397/93‑1 (MQ=255)

                            cAGCGTTACCAGGGGCTTGCCCAGAGAGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCaa                                                             >  1:1504699/1‑93 (MQ=255)

                             aGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAAc                                                            <  2:3727490/93‑1 (MQ=255)

                              gCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACt                                                           >  1:3818907/1‑93 (MQ=255)

                              gCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACt                                                           >  1:2705124/1‑93 (MQ=255)

                              gCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACt                                                           <  1:840357/93‑1 (MQ=255)

                                gTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTgg                                                         <  2:3018299/93‑1 (MQ=255)

                                  tACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGcc                                                       <  2:1383125/93‑1 (MQ=255)

                                  tACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGcc                                                       <  2:974464/93‑1 (MQ=255)

                                   aCCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCa                                                      >  1:2520400/1‑93 (MQ=255)

                                   aCCAGGGGCTTGCCCAGAGCGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCa                                                      <  2:2334566/93‑1 (MQ=255)

                                    ccAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCaa                                                     <  1:3364277/93‑1 (MQ=255)

                                    ccAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCaa                                                     >  1:4255991/1‑93 (MQ=255)

                                     cAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAAc                                                    <  1:1045104/93‑1 (MQ=255)

                                      aGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACg                                                   >  2:653718/1‑93 (MQ=255)

                                       ggggCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGa                                                  >  1:1175801/1‑93 (MQ=255)

                                        gggCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGaa                                                 <  1:4381583/93‑1 (MQ=255)

                                         ggCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAg                                                >  1:3944591/1‑93 (MQ=255)

                                          gCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCGACGAAGc                                               >  2:553548/1‑93 (MQ=255)

                                          gCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGc                                               <  2:3018764/93‑1 (MQ=255)

                                           cTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGcc                                              <  2:1907504/93‑1 (MQ=255)

                                           cTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGcc                                              <  2:3379686/93‑1 (MQ=255)

                                           cTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGcc                                              <  2:3379685/93‑1 (MQ=255)

                                            ttGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCg                                             >  1:1057851/1‑93 (MQ=255)

                                               cccAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGag                                          >  2:4459788/1‑93 (MQ=255)

                                                ccAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagt                                         >  1:2510224/1‑93 (MQ=255)

                                                ccAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagt                                         >  2:1307092/1‑93 (MQ=255)

                                                 cAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtc                                        >  1:3671125/1‑93 (MQ=255)

                                                 cAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtc                                        >  2:275807/1‑93 (MQ=255)

                                                 cAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtc                                        <  1:1954997/93‑1 (MQ=255)

                                                  agagTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtca                                       <  1:1040338/93‑1 (MQ=255)

                                                    agTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagt                                     <  2:2230714/93‑1 (MQ=255)

                                                     gTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCACTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtc                                    >  2:3886755/1‑93 (MQ=255)

                                                      tGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtca                                   >  2:1287733/1‑93 (MQ=255)

                                                      tGATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtca                                   >  1:260440/1‑93 (MQ=255)

                                                       gATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtcag                                  >  2:3284004/1‑93 (MQ=255)

                                                       gATAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtcag                                  <  2:2173805/93‑1 (MQ=255)

                                                         tAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtcagtc                                <  1:2136253/93‑1 (MQ=255)

                                                         tAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtcagtc                                >  1:4078436/1‑93 (MQ=255)

                                                          aaaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTcc                               >  2:4338103/1‑93 (MQ=255)

                                                          aaaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTcc                               >  2:343623/1‑93 (MQ=255)

                                                           aaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCa                              >  2:461679/1‑93 (MQ=255)

                                                            aaaaCTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAGCTGGCCAACGAAGCCGGAGTCAGTCAGTCCAg                             <  2:3192221/93‑1 (MQ=255)

                                                            aaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAg                             <  1:536105/93‑1 (MQ=255)

                                                            aaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAg                             >  2:2815463/1‑93 (MQ=255)

                                                             aaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGc                            <  2:1021119/93‑1 (MQ=255)

                                                               aCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGcgt                          >  1:2009858/1‑93 (MQ=255)

                                                                 tGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGcgtcg                        <  2:1946084/93‑1 (MQ=255)

                                                                 tGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGcgtcg                        >  2:4167661/1‑93 (MQ=255)

                                                                  ggCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGcgtcgt                       <  1:4122255/93‑1 (MQ=255)

                                                                  ggCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGcgtcgt                       >  2:1666092/1‑93 (MQ=255)

                                                                  ggCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGcgtcgt                       <  1:3041489/93‑1 (MQ=255)

                                                                     ggATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCCGCGTCGTGaa                    >  1:3197474/1‑93 (MQ=255)

                                                                     ggATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGaa                    <  1:2679350/93‑1 (MQ=255)

                                                                      gATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGacg                   >  2:111326/1‑91 (MQ=255)

                                                                      gATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAg                   >  2:111325/1‑93 (MQ=255)

                                                                       gttATCTGCTGCTACAACCCGATACTGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGt                  <  2:4661294/92‑1 (MQ=255)

                                                                       aTTATCTGCTGCTACACCCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGt                  <  2:2422403/93‑1 (MQ=255)

                                                                        ttATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGtt                 >  2:2228124/1‑93 (MQ=255)

                                                                        ttATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGtt                 >  2:3451947/1‑93 (MQ=255)

                                                                         tATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTc                <  1:1017745/93‑1 (MQ=255)

                                                                          aTCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTcg               <  1:735669/93‑1 (MQ=255)

                                                                          aTCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGACAGTCAGTCCAGCGTCGTGAAGTTcg               >  2:2350009/1‑93 (MQ=255)

                                                                           tCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTcgc              >  2:3724406/1‑93 (MQ=255)

                                                                           tCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTcgc              <  2:2560685/93‑1 (MQ=255)

                                                                             tgctgcTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCgcgc            >  1:1097596/1‑93 (MQ=255)

                                                                             tgctgcTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCgcgc            >  1:1896695/1‑93 (MQ=255)

                                                                                tgctACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaa         <  1:1384794/93‑1 (MQ=255)

                                                                                 gctACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaaa        >  1:4568901/1‑93 (MQ=255)

                                                                                  ctACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaaaa       <  1:804936/93‑1 (MQ=255)

                                                                                   tACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAAc      >  1:1382012/1‑93 (MQ=255)

                                                                                   tACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAAc      >  1:2829791/1‑93 (MQ=255)

                                                                                     cAACCCGATGCGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTc    <  2:3467466/93‑1 (MQ=255)

                                                                                     cAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACtg    >  1:2338928/1‑92 (MQ=255)

                                                                                      aaCCCGATACGGCGCGCCATTTAAGCTTTCAGCACCGGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCg   <  1:3537356/93‑1 (MQ=255)

                                                                                      aaCCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCg   <  1:4427848/93‑1 (MQ=255)

                                                                                      aaCCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCg   <  2:2394571/93‑1 (MQ=255)

                                                                                      aaCCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCg   >  2:92798/1‑93 (MQ=255)

                                                                                      aaCCCGATACGGCGCGCCATATAAGCTCTCAGCAACTGCGCAACAAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGAAAAAACTCg   <  2:2361674/93‑1 (MQ=255)

AGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGG  >  NC_000913/2696775‑2696954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`