BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,718,460	T→C	I163T (ATC→ACC)	yfiQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,718,460	0	T	C	99.2%	405.1 / ‑4.4	127	I163T (ATC→ACC)	yfiQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (61/65); total (62/65)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01

Only 100 of 139 total aligned reads displayed.

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACT  >  NC_000913/2718370‑2718552

ggCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAccc                                                                                            <  1:2279431/93‑1 (MQ=255)

ggCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAccc                                                                                            >  1:3589012/1‑93 (MQ=255)

 gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCt                                                                                           >  1:4318734/1‑93 (MQ=255)

  cAAGGGCGGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCGCCCTc                                                                                          <  2:603617/93‑1 (MQ=255)

   aaGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCg                                                                                         >  2:434382/1‑93 (MQ=255)

   aaGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCg                                                                                         <  1:1072369/93‑1 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGGCGTCTCCAACACCACCCTCGa                                                                                        >  1:3004254/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                        >  1:1260634/1‑93 (MQ=255)

     ggTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGAc                                                                                       <  1:4276683/93‑1 (MQ=255)

       tCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTg                                                                                     >  1:511518/1‑93 (MQ=255)

         tGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTggg                                                                                   <  2:3483031/93‑1 (MQ=255)

          gAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgc                                                                                  >  1:3642262/1‑93 (MQ=255)

          gAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgc                                                                                  >  2:3996394/1‑93 (MQ=255)

          gAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTAGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgc                                                                                  >  1:340741/1‑93 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                             <  2:1335212/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                             <  1:1316505/93‑1 (MQ=255)

                cAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACa                                                                            <  1:885187/93‑1 (MQ=255)

                cAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACa                                                                            <  2:4447380/93‑1 (MQ=255)

                 cgCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAg                                                                           <  1:2778908/92‑1 (MQ=255)

                  gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGc                                                                          >  1:91488/1‑93 (MQ=255)

                     tttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTa                                                                       <  1:2260366/93‑1 (MQ=255)

                      ttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTaa                                                                      <  2:97772/93‑1 (MQ=255)

                      ttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTaa                                                                      <  2:97771/93‑1 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                    >  1:4135803/1‑93 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGAATGATGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACTCCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                    <  2:3422386/93‑1 (MQ=255)

                         gCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAt                                                                   <  1:2117330/93‑1 (MQ=255)

                          ccTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATg                                                                  <  2:2379915/93‑1 (MQ=255)

                          ccTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATg                                                                  <  1:221375/93‑1 (MQ=255)

                           cTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATgg                                                                 >  1:344196/1‑93 (MQ=255)

                           cTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATgg                                                                 >  2:3525136/1‑93 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                                >  2:741309/1‑93 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                                >  1:56577/1‑93 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                                >  1:3298656/1‑93 (MQ=255)

                             gtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGc                                                               <  1:3792207/93‑1 (MQ=255)

                                ccGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCttt                                                            >  1:4682406/1‑93 (MQ=255)

                                 cGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCtttt                                                           <  2:3546783/93‑1 (MQ=255)

                                 cGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCtttt                                                           >  1:3345759/1‑93 (MQ=255)

                                 cGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCtttt                                                           >  2:3479451/1‑93 (MQ=255)

                                  gATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTc                                                          >  1:1643644/1‑93 (MQ=255)

                                   aTTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTcc                                                         <  2:384120/93‑1 (MQ=255)

                                     tAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTa                                                       >  1:2037650/1‑93 (MQ=255)

                                      aaaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAc                                                      <  1:1678090/93‑1 (MQ=255)

                                       atcgcgGCTAGCTGGCTTTTATTTCGCTATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACt                                                     <  2:1836582/91‑1 (MQ=255)

                                         cgcgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACttt                                                   >  1:2080371/1‑93 (MQ=255)

                                         cgcgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACttt                                                   <  2:2627172/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                                >  2:851892/1‑93 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                                >  1:3188034/1‑93 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                                >  2:3639102/1‑93 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                                <  1:2392005/93‑1 (MQ=255)

                                             gCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTg                                               <  2:3598773/93‑1 (MQ=255)

                                             gCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTg                                               >  2:3115615/1‑93 (MQ=255)

                                              cAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgc                                              <  1:1760674/93‑1 (MQ=255)

                                               aaccTGGCGTTTATTTCGCAATCGGCTGCTGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcg                                             >  2:1605980/4‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                            >  2:2844149/1‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                            <  2:2820729/93‑1 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                            >  1:1908505/1‑93 (MQ=255)

                                                 gCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCt                                           <  2:788229/93‑1 (MQ=255)

                                                 gCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCt                                           >  2:4048603/1‑93 (MQ=255)

                                                  cTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTc                                          <  2:4163809/93‑1 (MQ=255)

                                                   tGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCg                                         <  1:4106853/93‑1 (MQ=255)

                                                   tGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCg                                         <  1:2393279/93‑1 (MQ=255)

                                                   tGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCg                                         <  1:2591937/93‑1 (MQ=255)

                                                   tGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCg                                         >  1:3690485/1‑93 (MQ=255)

                                                   tGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCg                                         <  1:1531672/93‑1 (MQ=255)

                                                     gCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGc                                       <  1:637638/93‑1 (MQ=255)

                                                      cGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCg                                      <  2:3441759/93‑1 (MQ=255)

                                                       gTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGa                                     >  1:3830161/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                    >  1:2809744/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                    >  1:3931716/1‑93 (MQ=255)

                                                         ttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACa                                   >  1:1521201/1‑93 (MQ=255)

                                                           aTTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGc                                 >  2:289566/1‑93 (MQ=255)

                                                           aTTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGc                                 <  2:290505/93‑1 (MQ=255)

                                                            tttCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCACAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGcc                                <  1:2568680/93‑1 (MQ=255)

                                                             ttCGCAATCGGCTGCCGTCTCCAACACCTCCCTCGACTGGGCGCAACAGCGTAAGATAGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCt                               <  1:2665849/93‑1 (MQ=255)

                                                             ttCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCt                               <  1:42058/93‑1 (MQ=255)

                                                             ttCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCt                               <  2:466932/93‑1 (MQ=255)

                                                             ttCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCt                               <  2:3038426/93‑1 (MQ=255)

                                                              tCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTg                              <  2:873484/93‑1 (MQ=255)

                                                                gCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGa                            <  2:4477018/93‑1 (MQ=255)

                                                                 cAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGat                           <  2:2829284/93‑1 (MQ=255)

                                                                   aTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCACCAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGatat                         >  1:3861089/1‑93 (MQ=255)

                                                                   aTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGatat                         <  1:821629/93‑1 (MQ=255)

                                                                      ggCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGa                      <  1:2300175/93‑1 (MQ=255)

                                                                       gCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAc                     >  1:1172822/1‑93 (MQ=255)

                                                                       gCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAc                     <  1:2797021/93‑1 (MQ=255)

                                                                        cTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACg                    <  1:93069/93‑1 (MQ=255)

                                                                        cTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACg                    >  1:143787/1‑93 (MQ=255)

                                                                          gCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGtt                  >  1:3661952/1‑93 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat               >  1:3378261/1‑93 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat               >  1:4372106/1‑93 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat               <  1:1916698/93‑1 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat               >  2:1010076/1‑93 (MQ=255)

                                                                              tctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg              >  1:3087468/1‑93 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa            <  1:2740808/93‑1 (MQ=255)

                                                                                  cAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGCTGAAtt          >  1:1421724/1‑93 (MQ=255)

                                                                                  cAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAtt          <  1:2369317/93‑1 (MQ=255)

                                                                                   aaCACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACATCCTGGATATCGACGTTGATGAATTg         >  2:1040179/1‑93 (MQ=255)

                                                                                    acacCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGc        >  1:3384119/1‑93 (MQ=255)

                                                                                       ccACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTg     >  2:354087/1‑93 (MQ=255)

                                                                                          tCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACt  >  1:2336496/1‑93 (MQ=255)

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACT  >  NC_000913/2718370‑2718552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`