BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,746,200	T→C	S73S (AGT→AGC)	b2611 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,746,200	0	T	C	97.0%	411.2 / 1.5	134	S73S (AGT→AGC)	b2611	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/3); new base C (57/72); total (58/76)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 139 total aligned reads displayed.

TGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACC  >  NC_000913/2746111‑2746291

 gCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGTTtga                                                                                         >  2:2214366/1‑93 (MQ=255)

 gCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtga                                                                                         <  1:553615/93‑1 (MQ=255)

   cACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtgatg                                                                                       >  2:2949438/1‑93 (MQ=255)

   cACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtgatg                                                                                       >  2:3199332/1‑93 (MQ=255)

   cACGCAATCGCTCAGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtgatg                                                                                       >  1:3136712/1‑93 (MQ=255)

    aCGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtgatga                                                                                      <  2:2835391/93‑1 (MQ=255)

    aCGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACATCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtgatga                                                                                      >  1:1756091/1‑93 (MQ=255)

      gCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATc                                                                                    <  1:4231355/93‑1 (MQ=255)

        aaTCGCTCTGGAAGCCCGCATCCTGCCCGACTGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTg                                                                                  <  1:415182/93‑1 (MQ=255)

        aaTCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTg                                                                                  <  2:4193713/93‑1 (MQ=255)

        aaTCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTg                                                                                  >  1:1803142/1‑93 (MQ=255)

          tCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTa                                                                                <  2:128556/93‑1 (MQ=255)

           cGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTAc                                                                               >  2:1115525/1‑93 (MQ=255)

           cGCCCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTAc                                                                               <  2:2381314/93‑1 (MQ=255)

            gCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACg                                                                              >  1:11143/1‑93 (MQ=255)

              tctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGt                                                                            <  2:1547107/93‑1 (MQ=255)

              tctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGt                                                                            <  2:1043938/93‑1 (MQ=255)

              tctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGt                                                                            >  1:4480868/1‑93 (MQ=255)

              cctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGt                                                                            <  1:1419162/92‑1 (MQ=255)

               ctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTa                                                                           <  1:4502517/93‑1 (MQ=255)

               ctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTa                                                                           <  2:4496899/93‑1 (MQ=255)

               ctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTa                                                                           <  1:671337/93‑1 (MQ=255)

                 ggAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAAt                                                                         >  2:2646935/1‑93 (MQ=255)

                 ggAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAAt                                                                         <  1:1094722/93‑1 (MQ=255)

                  gAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATg                                                                        <  1:2095814/93‑1 (MQ=255)

                    aGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCATCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGAc                                                                      <  2:2457017/93‑1 (MQ=255)

                    aGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGAc                                                                      <  2:1166516/93‑1 (MQ=255)

                     gCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGAcc                                                                     >  2:2138029/1‑93 (MQ=255)

                       ccGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCAt                                                                   >  1:2081644/1‑93 (MQ=255)

                        cGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGTCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCAtt                                                                  <  1:511208/93‑1 (MQ=255)

                         ccATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATtg                                                                 <  2:3756178/92‑1 (MQ=255)

                          cATGCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATtgt                                                                <  2:897616/93‑1 (MQ=255)

                          cATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATtgt                                                                <  1:1802894/93‑1 (MQ=255)

                          cATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATtgt                                                                <  2:961504/93‑1 (MQ=255)

                           aTCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATtgtg                                                               <  2:800739/93‑1 (MQ=255)

                            tCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTgg                                                              >  1:2433317/1‑93 (MQ=255)

                             ccTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGc                                                             <  2:338552/93‑1 (MQ=255)

                              cTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCt                                                            >  2:909143/1‑93 (MQ=255)

                               tGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCtt                                                           <  2:341459/93‑1 (MQ=255)

                               tGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCtt                                                           >  1:2999461/1‑93 (MQ=255)

                               tGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCtt                                                           >  1:2287256/1‑93 (MQ=255)

                               tGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGAACATTGTGGCtt                                                           >  2:2125584/1‑93 (MQ=255)

                                gCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTtc                                                          <  1:116638/93‑1 (MQ=255)

                                gCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTtc                                                          <  2:2038954/93‑1 (MQ=255)

                                gCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTtc                                                          <  1:40301/93‑1 (MQ=255)

                                  ccGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTtctc                                                        <  1:4370470/93‑1 (MQ=255)

                                   cGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCg                                                       >  2:4496424/1‑93 (MQ=255)

                                    gACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGc                                                      >  2:3052133/1‑93 (MQ=255)

                                    gACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGc                                                      <  1:2256472/93‑1 (MQ=255)

                                     aCGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCa                                                     >  1:4361629/1‑93 (MQ=255)

                                        gTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATc                                                  <  1:4490150/93‑1 (MQ=255)

                                         tGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCg                                                 >  1:94032/1‑93 (MQ=255)

                                          gATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGt                                                >  2:3035212/1‑93 (MQ=255)

                                           aTAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGATTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtg                                               <  2:4381025/93‑1 (MQ=255)

                                             aGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGAACATTGTGGCTTCTCGCAATCGtggc                                             <  2:4036587/93‑1 (MQ=255)

                                                ggACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtggctgg                                          >  2:2004525/1‑93 (MQ=255)

                                                 gACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtggctggc                                         <  1:2891114/93‑1 (MQ=255)

                                                 gACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtggctggc                                         <  1:1856954/93‑1 (MQ=255)

                                                 gACAAAACCTCAGCCTGCTGAACGTTGGGTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtggcgggc                                         >  2:4445333/1‑93 (MQ=255)

                                                       aCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctgc                                   <  1:3408186/93‑1 (MQ=255)

                                                       aCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctgc                                   >  2:2994510/1‑93 (MQ=255)

                                                       aCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctgc                                   <  1:3408187/93‑1 (MQ=255)

                                                       aCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctgc                                   <  2:2071952/93‑1 (MQ=255)

                                                        ccTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctggt                                  >  2:4624814/1‑91 (MQ=255)

                                                        ccTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctgct                                  <  1:1556878/93‑1 (MQ=255)

                                                          tCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTAc                                >  1:238969/1‑93 (MQ=255)

                                                          tCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTAc                                <  2:3971419/93‑1 (MQ=255)

                                                          tCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTAc                                >  1:3077612/1‑93 (MQ=255)

                                                             gCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTAccct                             >  2:1699025/1‑92 (MQ=255)

                                                             gCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCa                             <  1:3171709/93‑1 (MQ=255)

                                                              ccTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCAt                            <  1:98473/93‑1 (MQ=255)

                                                               ctgctgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCAtt                           >  2:3067324/1‑93 (MQ=255)

                                                               ctgctgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCAtt                           <  1:2077555/93‑1 (MQ=255)

                                                                tgctgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTg                          >  1:1057153/1‑93 (MQ=255)

                                                                tgctgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTg                          >  1:2716354/1‑93 (MQ=255)

                                                                  ctgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTc                        <  1:2672496/93‑1 (MQ=255)

                                                                    gAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTa                      >  2:3571198/1‑93 (MQ=255)

                                                                    gAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTa                      <  2:2414245/93‑1 (MQ=255)

                                                                    gAACGTTGGTTCATTGGTCAGCGTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTa                      <  2:4706467/93‑1 (MQ=255)

                                                                      aCGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATg                    >  1:3274557/1‑93 (MQ=255)

                                                                      aCGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATg                    >  1:1212053/1‑93 (MQ=255)

                                                                        gTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGcc                  >  1:3999581/1‑93 (MQ=255)

                                                                          tGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCtt                <  1:824559/93‑1 (MQ=255)

                                                                           ggTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCttt               <  2:1815886/93‑1 (MQ=255)

                                                                           ggTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCttt               >  1:2611502/1‑93 (MQ=255)

                                                                           ggTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCttt               <  2:411425/93‑1 (MQ=255)

                                                                           ggTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCttt               <  1:191571/93‑1 (MQ=255)

                                                                            gTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTg              <  1:3795919/93‑1 (MQ=255)

                                                                            gTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTg              <  2:1364704/93‑1 (MQ=255)

                                                                            gTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTg              >  1:4083194/1‑93 (MQ=255)

                                                                             ttCATTGGTCAGCTTGATGATCTGTACGGTAATTACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTgc             >  1:523022/1‑93 (MQ=255)

                                                                             ttCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTgc             <  1:1275273/93‑1 (MQ=255)

                                                                             ttCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTgc             >  1:2811255/1‑93 (MQ=255)

                                                                              tCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTgcg            >  1:3832748/1‑93 (MQ=255)

                                                                               cATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTgcgc           <  1:2722386/93‑1 (MQ=255)

                                                                                aTTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCt          <  2:310382/93‑1 (MQ=255)

                                                                                   tgTCAGCTTGATGATCTGTGCGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTAt       <  1:3175220/92‑1 (MQ=255)

                                                                                     tCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCa     >  2:3660293/1‑93 (MQ=255)

                                                                                     tCAGCTGGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCa     <  1:42662/93‑1 (MQ=255)

                                                                                        gTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAAcc  <  2:646830/93‑1 (MQ=255)

TGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACC  >  NC_000913/2746111‑2746291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`