BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,748,983	T→C	A44A (GCT→GCC)	yfjB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,748,983	0	T	C	98.9%	601.8 / ‑4.3	188	A44A (GCT→GCC)	yfjB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (88/98); total (90/98)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 191 total aligned reads displayed.

ACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGAC  >  NC_000913/2748893‑2749070

aCGGCCCCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCa                                                                                       <  1:1014810/93‑1 (MQ=255)

aCGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCa                                                                                       <  1:1014811/93‑1 (MQ=255)

aCGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCa                                                                                       <  1:2614523/93‑1 (MQ=255)

    cACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGaa                                                                                   >  1:1567224/1‑93 (MQ=255)

      ccccACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACt                                                                                 >  2:3588178/1‑93 (MQ=255)

      ccccACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACt                                                                                 <  1:4030094/93‑1 (MQ=255)

      ccccACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACt                                                                                 >  1:1870740/1‑93 (MQ=255)

       cccACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTg                                                                                >  1:344938/1‑93 (MQ=255)

       cccACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTg                                                                                >  2:1998034/1‑93 (MQ=255)

          actgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCaa                                                                             >  2:2021562/1‑93 (MQ=255)

          actgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCaa                                                                             >  2:2504919/1‑93 (MQ=255)

           ctgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAAc                                                                            <  2:4685525/93‑1 (MQ=255)

            tgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACt                                                                           <  1:3023512/93‑1 (MQ=255)

             gcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTg                                                                          <  2:2279464/93‑1 (MQ=255)

               actgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaa                                                                        >  2:1770095/1‑93 (MQ=255)

               actgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaa                                                                        >  2:3797493/1‑93 (MQ=255)

                ctgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaaa                                                                       >  1:982481/1‑92 (MQ=255)

                 tgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaaga                                                                      >  1:4644336/1‑93 (MQ=255)

                  gACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaagaa                                                                     <  1:1931868/93‑1 (MQ=255)

                  gACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaagaa                                                                     <  2:837928/93‑1 (MQ=255)

                   acaacaCATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAAt                                                                    >  2:1266649/1‑93 (MQ=255)

                   acaacaCATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAAt                                                                    <  1:3155978/93‑1 (MQ=255)

                    caacaCATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAAtg                                                                   >  2:2475303/1‑93 (MQ=255)

                      acacATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAAtgtg                                                                 >  1:450180/1‑93 (MQ=255)

                      acacATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAAtgtg                                                                 >  2:114674/1‑93 (MQ=255)

                       cacaTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGa                                                                <  2:367740/93‑1 (MQ=255)

                        acaTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGaa                                                               >  1:434230/1‑93 (MQ=255)

                         caTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGaaa                                                              <  2:1620561/93‑1 (MQ=255)

                          aTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGTGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGaaaa                                                             >  1:3748394/1‑93 (MQ=255)

                          aTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGaaaa                                                             <  1:1175532/93‑1 (MQ=255)

                             aaaTGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTg                                                          <  2:4504407/93‑1 (MQ=255)

                             aaaTGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTg                                                          <  2:4273021/93‑1 (MQ=255)

                             aaaTGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTg                                                          <  1:1829387/93‑1 (MQ=255)

                             aaaTGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTg                                                          >  2:3030909/1‑93 (MQ=255)

                                tGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCa                                                       <  1:1652406/93‑1 (MQ=255)

                                tGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCa                                                       <  1:3501505/93‑1 (MQ=255)

                                  ctctACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACg                                                     >  1:590575/1‑93 (MQ=255)

                                   tctACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGc                                                    <  2:912779/93‑1 (MQ=255)

                                   tctACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGc                                                    >  2:1788382/1‑93 (MQ=255)

                                   tctACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGc                                                    >  2:4597832/1‑93 (MQ=255)

                                   tctACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGc                                                    <  1:1243508/93‑1 (MQ=255)

                                    ctACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCt                                                   <  1:330915/93‑1 (MQ=255)

                                      aCCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTcg                                                 <  2:2292100/93‑1 (MQ=255)

                                      aCCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTcg                                                 >  1:3218493/1‑93 (MQ=255)

                                       ccGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTcgc                                                >  2:1474453/1‑93 (MQ=255)

                                        cGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTcgcg                                               >  1:3635155/1‑93 (MQ=255)

                                         gctggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCgg                                              >  1:3687261/1‑93 (MQ=255)

                                          ctggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAGCTGGCACGCTCGCGga                                             <  2:4366574/93‑1 (MQ=255)

                                          ctggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGga                                             <  2:3561893/93‑1 (MQ=255)

                                          ctggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGga                                             >  1:3007867/1‑93 (MQ=255)

                                            ggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGgaga                                           >  1:3111150/1‑93 (MQ=255)

                                             gctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGAt                                          <  1:4124938/93‑1 (MQ=255)

                                             gctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGAt                                          <  2:2949437/93‑1 (MQ=255)

                                             gctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGAt                                          <  1:1444605/93‑1 (MQ=255)

                                               tgtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTg                                        >  2:152038/1‑93 (MQ=255)

                                                gtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTgg                                       >  1:4735265/1‑93 (MQ=255)

                                                gtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTgg                                       >  2:3877629/1‑93 (MQ=255)

                                                gtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTgg                                       <  2:4674613/93‑1 (MQ=255)

                                                gtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTgg                                       >  2:1362685/1‑93 (MQ=255)

                                                 tgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTggg                                      <  1:3730322/93‑1 (MQ=255)

                                                   cacaAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCa                                    >  2:3168512/1‑93 (MQ=255)

                                                    acaAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCaa                                   >  2:3301877/1‑93 (MQ=255)

                                                     caAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGTTTGGGCAAc                                  >  2:1099947/1‑93 (MQ=255)

                                                     caAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAAc                                  <  2:1838690/93‑1 (MQ=255)

                                                     caAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAAc                                  <  2:3119335/93‑1 (MQ=255)

                                                      aaaaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACt                                 <  1:3589016/93‑1 (MQ=255)

                                                      aaaaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACt                                 <  2:2454276/93‑1 (MQ=255)

                                                       aaaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTa                                <  2:1572244/93‑1 (MQ=255)

                                                       aaaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTa                                <  1:4092904/93‑1 (MQ=255)

                                                        aaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAg                               >  1:2072062/1‑93 (MQ=255)

                                                          ggTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTCGCt                             >  1:1746511/1‑93 (MQ=255)

                                                            ttACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGa                           <  2:4327556/93‑1 (MQ=255)

                                                             tACGAGGTCATCGTTGAGCAACAAATCGCCCAGGAACTGCAACTGAAGAATGTTAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGAt                          >  2:3943470/1‑93 (MQ=255)

                                                               cGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGAtct                        >  1:2210946/1‑93 (MQ=255)

                                                                gAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGAtctc                       >  1:2893449/1‑93 (MQ=255)

                                                                gAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGAtctc                       <  1:1341012/93‑1 (MQ=255)

                                                                 aGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcg                      >  1:1278534/1‑93 (MQ=255)

                                                                 aGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcg                      >  1:379743/1‑93 (MQ=255)

                                                                 aGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcg                      <  2:1203854/93‑1 (MQ=255)

                                                                   gTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcgcg                    >  1:1692959/1‑93 (MQ=255)

                                                                    tCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCgg                   <  2:3476148/93‑1 (MQ=255)

                                                                    tCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCgg                   <  1:639415/93‑1 (MQ=255)

                                                                     cATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGt                  >  2:1164329/1‑93 (MQ=255)

                                                                       tCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAg                <  2:430895/93‑1 (MQ=255)

                                                                       tCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAg                >  1:4167379/1‑93 (MQ=255)

                                                                       tCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAg                <  1:242972/93‑1 (MQ=255)

                                                                         gTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTc              >  2:375750/1‑93 (MQ=255)

                                                                          ttGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGTAACTAGCTGATCTCGCGGTAGTCg             >  2:1709973/1‑93 (MQ=255)

                                                                            gAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGtt           >  2:4197856/1‑93 (MQ=255)

                                                                              gCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtgg         <  2:2686144/93‑1 (MQ=255)

                                                                                aacaaATCGCTCACGAACTGCAACTGAAGAATGAGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtg       <  2:4114086/93‑1 (MQ=255)

                                                                                aacaaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtg       <  1:3871726/93‑1 (MQ=255)

                                                                                aacaaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtg       >  1:4266737/1‑93 (MQ=255)

                                                                                 acaaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtgg      >  2:306625/1‑93 (MQ=255)

                                                                                 acaaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtgg      >  1:296580/1‑93 (MQ=255)

                                                                                 acaaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtgg      >  2:672848/1‑93 (MQ=255)

                                                                                  caaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGc     <  2:2836544/93‑1 (MQ=255)

                                                                                  caaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGc     <  2:4455526/93‑1 (MQ=255)

                                                                                  caaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGc     <  2:506731/93‑1 (MQ=255)

                                                                                   aaaTCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCg    <  2:1015054/93‑1 (MQ=255)

ACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGAC  >  NC_000913/2748893‑2749070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`