| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,806,271 | T→C | intergenic (+126/‑1367) | proX → / → b2682 | high‑affinity transport system for glycine betaine and proline/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,806,271 | 0 | T | C | 96.8% | 476.6 / 3.1 | 155 | intergenic (+126/‑1367) | proX/b2682 | high‑affinity transport system for glycine betaine and proline/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (64/86); total (67/88) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
TCCGCCGTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTT > NC_000913/2806180‑2806360 | tCCGCCGTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATc > 1:3989810/1‑93 (MQ=255) cgccgTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCAcccc < 2:1759944/93‑1 (MQ=255) cgccgTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCAcccc > 1:591961/1‑93 (MQ=255) gccgTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCa > 1:3626371/1‑93 (MQ=255) gccgTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCa < 1:2686461/93‑1 (MQ=255) cgTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCata > 2:4581418/1‑93 (MQ=255) cgTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCata < 2:3889469/93‑1 (MQ=255) ttCTGTGCACAATGCCTGATGCGACGGTGGCGCGTCTTAACATTCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATAtt < 2:2677271/93‑1 (MQ=38) tCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATtc > 2:2528628/1‑93 (MQ=255) cTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATtca < 2:2812825/93‑1 (MQ=255) cTGTGCACAATGCCTGATGCGACGCTGGCGCGGCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCCCCCCATATtca < 1:4135788/93‑1 (MQ=37) tgtgCACAATGCCTGATGCGACGCTGGCGCGTCTTATCGTGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATtcat > 1:3047956/1‑93 (MQ=255) tgtgCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATtcat > 1:3132257/1‑93 (MQ=255) gtgCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATtcatc < 1:294720/93‑1 (MQ=255) tgCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATtcatca > 2:1572682/1‑93 (MQ=255) acaATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACa < 2:1864580/93‑1 (MQ=255) caATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACAt < 2:3271780/93‑1 (MQ=255) aTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATcc < 2:1439486/93‑1 (MQ=255) aTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATcc < 1:161820/93‑1 (MQ=255) gCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGc < 1:2898825/93‑1 (MQ=255) gCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGc < 2:2015948/93‑1 (MQ=255) gCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGc < 1:3524818/93‑1 (MQ=255) gCCTGATGCGACGCTGGCGCGTCTGATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGc < 1:1130615/93‑1 (MQ=255) ccTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCt > 2:3332199/1‑93 (MQ=255) gATGCGACGCTGGCGCGTCGTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCtatt < 1:2923014/93‑1 (MQ=255) tGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCtattat < 2:1053512/93‑1 (MQ=255) tGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCtattat > 1:4585032/1‑93 (MQ=255) tGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCtattat > 1:1238874/1‑93 (MQ=255) tGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCtattat < 1:1074310/93‑1 (MQ=255) gCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCAAATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTattatt > 2:2708555/1‑93 (MQ=255) cGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTg < 1:391712/93‑1 (MQ=255) gACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGa < 1:3302600/93‑1 (MQ=255) gACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGa > 1:2379698/1‑93 (MQ=255) gACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGa < 2:2296088/93‑1 (MQ=255) gACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGa < 2:3818247/93‑1 (MQ=255) aCGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGAt < 2:3200938/93‑1 (MQ=255) aCGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGAt > 1:4696773/1‑93 (MQ=255) aCGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGAt > 1:1270585/1‑93 (MQ=255) cGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGAtt < 1:3387115/93‑1 (MQ=255) gCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGAttt > 1:4165114/1‑93 (MQ=255) gCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGAttt < 2:129308/93‑1 (MQ=255) cTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTc > 2:2736508/1‑93 (MQ=255) tGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTcc > 2:2661391/1‑93 (MQ=255) ggCGCGTCTTATCATGCCTACAAACCGCATCGCACATTTCACCAATCTATCTTTCACCCCATATTCGTCAACATCCGCTAATATTGATTTCCa < 2:4216700/93‑1 (MQ=38) cgcgTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGc < 2:3142849/93‑1 (MQ=255) gcgTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCt > 2:1989080/1‑93 (MQ=255) cgTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCCCCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCtt < 1:1672635/93‑1 (MQ=255) gTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTa > 1:4537251/1‑93 (MQ=255) gTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTa > 2:1429116/1‑93 (MQ=255) tCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTaa > 2:2068784/1‑93 (MQ=255) tCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTaa > 2:2385765/1‑93 (MQ=255) tCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTaa > 2:3626236/1‑93 (MQ=255) cTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAat < 2:4605770/93‑1 (MQ=255) tATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAatca < 2:4495337/93‑1 (MQ=255) taTGCCTGAAAACCCCATCGCACTTTTCATCGAGCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAAtcatca < 1:1414884/92‑1 (MQ=255) aTGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCAc < 2:2516511/93‑1 (MQ=255) aTGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCAc > 1:4489910/1‑93 (MQ=255) tGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCAcc > 2:1796353/1‑93 (MQ=255) gCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCt < 2:2862968/93‑1 (MQ=255) gCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCt < 1:703824/93‑1 (MQ=255) cTACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtga < 2:3973090/93‑1 (MQ=255) tACAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtgat > 1:1404713/1‑93 (MQ=255) aCAAACCACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtgatg > 2:1270736/1‑93 (MQ=255) ccACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACaa < 2:1109634/93‑1 (MQ=255) ccACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACaa < 2:1109633/93‑1 (MQ=255) ccACATCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACaa > 2:3079145/1‑93 (MQ=255) cacaTCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACaaa > 1:4033701/1‑93 (MQ=255) cacaTCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACaaa > 1:3804416/1‑93 (MQ=255) caTCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACaaaaa > 1:1439128/1‑93 (MQ=255) tCGCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAaata > 1:472246/1‑93 (MQ=255) gCACATTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCCTCACATGATTAACAAAaataat > 2:3778781/1‑93 (MQ=255) acaTTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGa < 1:1017697/93‑1 (MQ=255) acaTTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGa < 2:1109489/93‑1 (MQ=255) acaTTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGa > 1:1883773/1‑93 (MQ=255) aTTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACt < 1:933583/93‑1 (MQ=255) aTTTCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACt < 2:4368113/93‑1 (MQ=255) tttCAACAATCTATCTTTCATCCCATATTCAGCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTa < 1:2338452/93‑1 (MQ=255) ttCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTaa < 2:1221822/93‑1 (MQ=255) ttCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTaa < 2:2183679/93‑1 (MQ=255) tCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTaaa > 2:1968533/1‑93 (MQ=255) tCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTaaa > 2:2405583/1‑93 (MQ=255) tCAACAATCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTaaa > 1:224631/1‑93 (MQ=255) aacaaTCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAAcc < 2:828597/93‑1 (MQ=255) aacaaTCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAAcc > 1:116857/1‑93 (MQ=255) acaaTCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCt > 2:3084607/1‑93 (MQ=255) aaTCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTaa < 1:3783022/93‑1 (MQ=255) aaTCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTaa < 1:3688939/93‑1 (MQ=255) aaTCTATCTTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTaa < 2:2111350/93‑1 (MQ=255) atctatctTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAAt > 2:4112964/1‑93 (MQ=255) atctatctTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAAt > 2:1919165/1‑93 (MQ=255) tctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATc < 2:2120785/93‑1 (MQ=255) ctatctTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCa > 1:4744487/1‑93 (MQ=255) atctTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATg < 2:4152110/93‑1 (MQ=255) tctTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAGAAATAATGACTAAACCTAATCATGa > 2:4680168/1‑93 (MQ=255) tctTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGa < 1:3583282/93‑1 (MQ=255) tctTTCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGa < 1:2975975/93‑1 (MQ=255) tttCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGc < 2:466503/93‑1 (MQ=255) ttCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCt < 2:4076008/93‑1 (MQ=255) tCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCtt < 1:744622/93‑1 (MQ=255) tCACCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCtt < 1:1592533/93‑1 (MQ=255) | TCCGCCGTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTT > NC_000913/2806180‑2806360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |