BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,102	T→C	F230L (TTC→CTC)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,102	0	T	C	100.0%	407.6 / NA	125	F230L (TTC→CTC)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (65/60); total (65/60)

Only 100 of 130 total aligned reads displayed.

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTA  >  NC_000913/2825012‑2825191

aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTtcctcg                                                                                         <  2:458674/93‑3 (MQ=255)

aTTGCCCACGGTCTTGCGCCGCTGGAGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTTCTCCTTCCCACTGCTTTCACCTttcctc                                                                                         >  2:4492416/1‑93 (MQ=255)

   gCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGc                                                                                      <  2:762722/93‑1 (MQ=255)

   gCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGc                                                                                      <  1:143527/93‑1 (MQ=255)

     ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                                                    <  1:653873/93‑1 (MQ=255)

      cACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                                                   >  1:4347041/1‑93 (MQ=255)

       ccGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                                                  <  2:3900689/92‑1 (MQ=255)

       aCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                                                  >  2:848519/1‑93 (MQ=255)

        cGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                                                 <  1:4065068/93‑1 (MQ=255)

         ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                                <  2:3590846/93‑1 (MQ=255)

         ggTCTTGATCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                                >  2:1549248/1‑93 (MQ=255)

          gTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                                               >  2:3406610/1‑93 (MQ=255)

          gTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                                               >  1:2284785/1‑93 (MQ=255)

            cTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCa                                                                             <  2:1734711/93‑1 (MQ=255)

             ttGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAg                                                                            >  2:1136390/1‑93 (MQ=255)

              tGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGt                                                                           <  2:3779470/93‑1 (MQ=255)

               gCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGtt                                                                          <  2:3265809/93‑1 (MQ=255)

                cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                                         <  1:2604037/93‑1 (MQ=255)

                cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCACACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                                         >  2:4137590/1‑93 (MQ=255)

                 tCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTAt                                                                        <  1:2673482/93‑1 (MQ=255)

                    gCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGc                                                                     >  1:427824/1‑93 (MQ=255)

                      tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                                                   >  2:702598/1‑93 (MQ=255)

                       ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                                                  <  2:469706/93‑1 (MQ=255)

                        gCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAg                                                                 >  1:2655319/1‑93 (MQ=255)

                           aGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGtt                                                              <  1:4275417/93‑1 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                             >  1:1549918/1‑93 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                             >  2:2762853/1‑93 (MQ=255)

                              cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           <  2:58836/93‑1 (MQ=255)

                               aTCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCg                                                          >  2:2334083/1‑93 (MQ=255)

                               aTCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCg                                                          >  1:1705194/1‑93 (MQ=255)

                                tCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                                         >  2:3775635/1‑93 (MQ=255)

                                 ccACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGc                                                        <  1:1023698/93‑1 (MQ=255)

                                        gtgtGGTCATGCTGGCGCTGATCGTCTCCTTCCCCCTTCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCCCAGGTTAGCGGCGTattga                                                 <  2:1948501/90‑1 (MQ=255)

                                        gTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattga                                                 <  2:3656290/93‑1 (MQ=255)

                                        gTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattga                                                 <  2:1849540/93‑1 (MQ=255)

                                         tCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgat                                                <  2:2367237/93‑1 (MQ=255)

                                         tCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgat                                                >  2:2540673/1‑93 (MQ=255)

                                          cTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgatt                                               <  1:2016997/93‑1 (MQ=255)

                                           tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                                              <  1:3368649/93‑1 (MQ=255)

                                            ggTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTgg                                             >  1:4585966/1‑93 (MQ=255)

                                             gTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGc                                            <  2:1190574/93‑1 (MQ=255)

                                             gTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGc                                            <  2:1453800/93‑1 (MQ=255)

                                             gTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGc                                            <  1:127378/93‑1 (MQ=255)

                                             gTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGc                                            >  1:4331712/1‑93 (MQ=255)

                                              tCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCg                                           >  2:2957445/1‑93 (MQ=255)

                                               cATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGt                                          <  2:3557142/93‑1 (MQ=255)

                                               cATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGt                                          >  1:2878339/1‑93 (MQ=255)

                                                   cTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAg                                      <  1:1756041/93‑1 (MQ=255)

                                                   cTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAg                                      <  1:3027389/93‑1 (MQ=255)

                                                    tGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGa                                     >  2:3100063/1‑93 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                    <  1:3251558/93‑1 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                    <  1:971404/93‑1 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                    <  1:223405/93‑1 (MQ=255)

                                                       cgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTg                                  <  2:3135978/93‑1 (MQ=255)

                                                        gcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTgg                                 >  1:1631693/1‑93 (MQ=255)

                                                        gcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTgg                                 >  1:1164281/1‑93 (MQ=255)

                                                          tCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc                               <  1:3126855/93‑1 (MQ=255)

                                                           cATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtct                              >  2:3775743/1‑93 (MQ=255)

                                                           cATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtct                              >  2:3321338/1‑93 (MQ=255)

                                                            aTCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtctc                             <  1:2854196/93‑1 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                            >  2:2335313/1‑93 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                            >  2:4573990/1‑93 (MQ=255)

                                                              cTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCgg                           >  2:2576549/1‑93 (MQ=255)

                                                              cTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCgg                           >  1:575276/1‑93 (MQ=255)

                                                               tgctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                          <  2:2030009/90‑1 (MQ=255)

                                                               tgctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                          <  1:1899149/90‑1 (MQ=255)

                                                                tctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCATATTGGTCTCGTCa                         <  2:2369665/91‑1 (MQ=255)

                                                                gctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCa                         <  1:4641796/91‑1 (MQ=255)

                                                                  tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat                       >  1:1454376/1‑93 (MQ=255)

                                                                  tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat                       <  1:285431/93‑1 (MQ=255)

                                                                    cttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAatat                     >  2:3888194/1‑93 (MQ=255)

                                                                     ttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAtt                    <  2:942015/93‑1 (MQ=255)

                                                                     ttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAtt                    >  2:2092962/1‑93 (MQ=255)

                                                                     ttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAtt                    <  1:1910447/93‑1 (MQ=255)

                                                                      tccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTc                   <  2:2472964/93‑1 (MQ=255)

                                                                      tccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTc                   <  2:158271/93‑1 (MQ=255)

                                                                        ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCGGATTGGTCTCGGCAATATTccg                 >  1:1048518/1‑93 (MQ=255)

                                                                        ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccg                 >  1:4294770/1‑93 (MQ=255)

                                                                        ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccg                 >  1:1048676/1‑93 (MQ=255)

                                                                           cTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgccg              >  2:207332/1‑93 (MQ=255)

                                                                           cTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgccg              >  2:207333/1‑93 (MQ=255)

                                                                            tGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCcgccgc             >  2:3589229/1‑93 (MQ=255)

                                                                            tGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCcgccgc             >  2:3879745/1‑93 (MQ=255)

                                                                             gCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCa            >  1:869436/1‑93 (MQ=255)

                                                                              cTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCAt           <  1:4355807/93‑1 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc          >  2:1033019/1‑93 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc          <  1:2091006/93‑1 (MQ=255)

                                                                                ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCt         <  1:1513171/93‑1 (MQ=255)

                                                                                ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCt         <  1:300653/93‑1 (MQ=255)

                                                                                ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCt         <  2:4426624/93‑1 (MQ=255)

                                                                                ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCt         >  1:2615885/1‑93 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg        <  2:2995948/93‑1 (MQ=255)

                                                                                   cccTTTCCCCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGc      <  2:1150574/92‑1 (MQ=255)

                                                                                   aCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGc      >  2:919175/1‑93 (MQ=255)

                                                                                   aCCTTTCCTC‑GCCCAGGCGCAGTTATCGCACAGGGTATCGGCGGATTGATTGGCGGGCAGATTGGTCTCGGCAATATTCCGCCGCCTCTGGCt     >  2:2976924/1‑93 (MQ=255)

                                                                                    ccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCt     >  1:1688730/1‑93 (MQ=255)

                                                                                    ccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCt     >  2:3012827/1‑93 (MQ=255)

                                                                                     cTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCtt    >  2:4595594/1‑93 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt   >  1:3005523/1‑93 (MQ=255)

                                                                                       ttcctcGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTa  <  1:1678490/93‑1 (MQ=255)

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTA  >  NC_000913/2825012‑2825191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`