BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,851,044	A→G	T296A (ACG→GCG)	hypD →	HypD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,851,044	0	A	G	100.0%	357.8 / NA	114	T296A (ACG→GCG)	hypD	HypD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (56/58); total (56/58)

Only 100 of 124 total aligned reads displayed.

GCGCAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGACGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTGTGGTGAG  >  NC_000913/2850954‑2851133

gcgcAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAGTGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCg                                                                                         >  2:4152822/1‑93 (MQ=255)

gcgcAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCg                                                                                         >  2:1211530/1‑93 (MQ=255)

   cAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCg                                                                                      >  1:715818/1‑93 (MQ=255)

    aaCAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCgg                                                                                     >  1:2261854/1‑93 (MQ=255)

     aCAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGa                                                                                    >  1:2654951/1‑93 (MQ=255)

      cAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGAt                                                                                   <  1:2875649/93‑1 (MQ=255)

      cAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAATCGTGAATGAATCTTCTGGCGTGCACCTGACGCCGGAt                                                                                   >  2:2937200/1‑93 (MQ=255)

       aGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGGACCTGGCGCCGGAtt                                                                                  >  2:4251472/1‑93 (MQ=255)

           gATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCa                                                                              <  2:3848949/93‑1 (MQ=255)

            aTTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGTACCTGGCGCCGGATTATCaa                                                                             >  1:3135362/1‑93 (MQ=255)

            aTTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCaa                                                                             <  2:1971998/93‑1 (MQ=255)

            aTTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCaa                                                                             <  2:3736290/93‑1 (MQ=255)

             ttGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAAc                                                                            <  1:559303/93‑1 (MQ=255)

                ccGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTTGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGAt                                                                         >  2:2260984/1‑93 (MQ=255)

                 cGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGAtt                                                                        >  2:83704/1‑93 (MQ=255)

                  gATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATTAACGATTc                                                                       <  2:369405/93‑1 (MQ=255)

                    tgtgTTCTGTGTTAACGGCGCCAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTTTGACCTGCACCTGGCGCCGGAGTATCAACGATTCGa                                                                     >  2:1708355/1‑93 (MQ=255)

                    tgtgTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGa                                                                     <  1:269898/93‑1 (MQ=255)

                    tgtgTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGa                                                                     <  1:738339/93‑1 (MQ=255)

                     gtgtTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGCTTCGAt                                                                    >  2:3723120/1‑93 (MQ=255)

                     gtgtTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGAt                                                                    <  2:4741340/93‑1 (MQ=255)

                     gtgtTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGAt                                                                    >  2:3723119/1‑93 (MQ=255)

                     gtgtTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGAt                                                                    >  2:4172728/1‑93 (MQ=255)

                       gtTCTGTGTCAACGGCGAGAGCGAATGGCGCGGCTTATGCGTGATTGAATCTTCTGCCGGGCCGCGCGCGCCGGCGTATCAACGATTCGATGc                                                                  >  2:2756187/1‑93 (MQ=255)

                       gtTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCCACTAGCGCCGGATTATCAACGATTCGATGc                                                                  >  2:507308/1‑93 (MQ=255)

                       gtTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGc                                                                  <  1:717972/93‑1 (MQ=255)

                        ttCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGcc                                                                 <  1:3802458/93‑1 (MQ=255)

                         tCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCg                                                                >  2:1867854/1‑93 (MQ=255)

                         tCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCg                                                                >  2:2140748/1‑93 (MQ=255)

                           tgtgTCAACGGCGACAGCGAATGGCGCGGCTTAGGGGTGATTGAATCTTTTGGGGGGCACTTGGCGCGGGATCATACACGATTCGATGCGGac                                                              >  1:1221405/1‑92 (MQ=255)

                           tgtgTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGaa                                                              >  1:2450070/1‑93 (MQ=255)

                           tgtgTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGaa                                                              <  1:2216089/93‑1 (MQ=255)

                           tgtgTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGaa                                                              >  1:913179/1‑93 (MQ=255)

                           tgtgTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGaa                                                              >  1:3281373/1‑93 (MQ=255)

                            gtgtCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGTATTATCAACGATTCGATGCCGAAg                                                             <  2:1501339/93‑1 (MQ=255)

                            gtgtCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAg                                                             <  2:309580/93‑1 (MQ=255)

                            gtgtCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAg                                                             <  1:3810361/93‑1 (MQ=255)

                             tgtCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGCTGCCGAAGc                                                            >  2:3235876/1‑93 (MQ=255)

                             tgtCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGc                                                            <  1:1331952/93‑1 (MQ=255)

                             tgtCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGc                                                            <  1:1331951/93‑1 (MQ=255)

                             agtCACCGGCGACAGCGAATGGCGCGACTTAGCCGTGATTGAATCTTCTGGCGTTCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGc                                                            <  2:1912829/92‑1 (MQ=255)

                              gtCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGca                                                           <  1:85554/93‑1 (MQ=255)

                               tCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGcac                                                          >  2:488746/1‑93 (MQ=255)

                                cagcGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCACCGATTCGATGCCGAAGcaca                                                         <  2:880858/90‑1 (MQ=255)

                                cAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGcaca                                                         <  2:1793655/93‑1 (MQ=255)

                                 aaCGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAt                                                        <  1:57594/93‑1 (MQ=255)

                                 aaCGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAt                                                        <  1:3772968/93‑1 (MQ=255)

                                  aCGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAtt                                                       <  2:2350885/93‑1 (MQ=255)

                                  aCGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAtt                                                       <  1:3464178/93‑1 (MQ=255)

                                   cGGCGACAGCGAATGGCGCGGCTTAGTCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAttt                                                      <  2:29621/93‑1 (MQ=255)

                                   cGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAttt                                                      <  1:1482736/93‑1 (MQ=255)

                                   cGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACAttt                                                      <  2:29622/93‑1 (MQ=255)

                                    ggCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTc                                                     <  1:2477987/93‑1 (MQ=255)

                                        aCAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGcc                                                 >  2:4429305/1‑93 (MQ=255)

                                         cAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGccc                                                >  2:3544482/1‑93 (MQ=255)

                                         cAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGccc                                                >  1:3424241/1‑93 (MQ=255)

                                         cAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCAGccc                                                >  1:2038868/1‑93 (MQ=255)

                                             gAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGACGCACATTTCCGCCCGGCa                                            >  1:3981989/1‑93 (MQ=255)

                                              aaTGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCAc                                           >  1:2026235/1‑93 (MQ=255)

                                               aTGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCAcc                                          >  2:1180869/1‑93 (MQ=255)

                                               aTGGCGCGACTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCAcc                                          <  2:1747614/93‑1 (MQ=255)

                                                    tcgGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCgcagc                                     >  1:4252718/2‑93 (MQ=255)

                                                    gcgGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCgcagc                                     <  2:3584579/93‑1 (MQ=255)

                                                    gcgGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCgcagc                                     <  1:1240495/93‑1 (MQ=255)

                                                         ttAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTc                                <  1:969742/93‑1 (MQ=255)

                                                          tAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCt                               >  2:108992/1‑93 (MQ=255)

                                                          tAGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCt                               <  2:4749296/93‑1 (MQ=255)

                                                           aGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTg                              <  2:900912/93‑1 (MQ=255)

                                                           aGGCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTg                              >  1:3180238/1‑93 (MQ=255)

                                                            ggCGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGc                             >  1:3707756/1‑93 (MQ=255)

                                                              ggTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGa                           <  1:680635/92‑1 (MQ=255)

                                                              cGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGa                           >  2:4724495/1‑93 (MQ=255)

                                                              cGTGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGa                           <  2:600722/93‑1 (MQ=255)

                                                              cGTGATTGAATCTTCTGGCATGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGa                           <  1:2970970/93‑1 (MQ=255)

                                                                tGATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATg                         >  1:668450/1‑93 (MQ=255)

                                                                 gATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGa                        <  1:1835476/93‑1 (MQ=255)

                                                                 gATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGa                        >  2:2460682/1‑93 (MQ=255)

                                                                 gATTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGa                        >  2:1210884/1‑93 (MQ=255)

                                                                  aTTGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGAc                       <  2:4080030/93‑1 (MQ=255)

                                                                   ttGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGAcc                      <  1:373032/93‑1 (MQ=255)

                                                                    tGAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGAccc                     <  2:3585892/93‑1 (MQ=255)

                                                                     gAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcg                    >  2:1131217/1‑93 (MQ=255)

                                                                     gAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcg                    >  1:2793440/1‑93 (MQ=255)

                                                                     gAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcg                    >  2:4461078/1‑93 (MQ=255)

                                                                     gAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcg                    <  1:4176531/93‑1 (MQ=255)

                                                                     gAATCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcg                    >  2:4443025/1‑93 (MQ=255)

                                                                       aTCTTCTGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcg                  >  1:2786218/1‑93 (MQ=255)

                                                                        tcttctGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcgc                 >  2:2484837/1‑93 (MQ=255)

                                                                        tcttctGGCGTGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCcgcgc                 >  1:4292184/1‑93 (MQ=255)

                                                                                  tGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTtgtg       >  1:4422734/1‑93 (MQ=255)

                                                                                  tGCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTtgtg       >  1:4719838/1‑93 (MQ=255)

                                                                                   gCACCTGGCTCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTgtgg      <  1:171423/93‑1 (MQ=255)

                                                                                   gCACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTgtgg      <  1:1797319/93‑1 (MQ=255)

                                                                                   gCACCTGCCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTgtgg      <  1:1797316/93‑1 (MQ=255)

                                                                                    cACCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTgtggt     <  1:4385512/93‑1 (MQ=255)

                                                                                     aCCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTgtggtg    <  2:2651031/93‑1 (MQ=255)

                                                                                     aCCTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTgtggtg    >  1:3048164/1‑93 (MQ=255)

                                                                                      ccTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTGTGGTGa   <  1:3517916/93‑1 (MQ=255)

                                                                                      ccTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTGTGGTGa   <  2:3510455/93‑1 (MQ=255)

                                                                                      ccTGGCGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTGTGGTGa   <  2:1871496/93‑1 (MQ=255)

GCGCAACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGCACCTGACGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGCGCGCGTTGTGGTGAG  >  NC_000913/2850954‑2851133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`