BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,862,683	T→C	V142A (GTT→GCT)	ygbM →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,862,683	0	T	C	95.7%	485.4 / 7.5	161	V142A (GTT→GCT)	ygbM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (3/4); new base C (78/76); total (81/80)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 168 total aligned reads displayed.

GCGAAGATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGTTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTGCGCGAGAT  >  NC_000913/2862593‑2862771

  gAAGATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGaa                                                                                      <  1:677999/93‑1 (MQ=255)

    aGATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAga                                                                                    >  1:3759185/1‑92 (MQ=255)

     gATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCa                                                                                   >  2:4242272/1‑93 (MQ=255)

     gATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCa                                                                                   >  2:4535275/1‑93 (MQ=255)

     gATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCa                                                                                   <  1:2635360/93‑1 (MQ=255)

     gATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCa                                                                                   >  1:2408908/1‑93 (MQ=255)

       tGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACt                                                                                 <  1:1792450/93‑1 (MQ=255)

       tGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACt                                                                                 >  2:2911286/1‑93 (MQ=255)

        gCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTc                                                                                <  2:128311/93‑1 (MQ=255)

        gCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTc                                                                                <  2:4250225/93‑1 (MQ=255)

           gAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGc                                                                             >  1:1225476/1‑93 (MQ=255)

           gAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGc                                                                             <  2:4294376/93‑1 (MQ=255)

           gAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGc                                                                             >  2:2985324/1‑93 (MQ=255)

            aaCGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGcc                                                                            <  2:1200256/93‑1 (MQ=255)

              cGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGcccc                                                                          >  2:1590272/1‑93 (MQ=255)

              cGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGcccc                                                                          >  1:958495/1‑93 (MQ=255)

              cGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCAATCAGcccc                                                                          >  2:3568068/1‑93 (MQ=255)

                gTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCgg                                                                        >  2:3108095/1‑93 (MQ=255)

                gTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCgg                                                                        >  1:4628129/1‑93 (MQ=255)

                  aCCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCg                                                                      >  1:2305154/1‑93 (MQ=255)

                   ccGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGt                                                                     >  2:3298316/1‑93 (MQ=255)

                   ccGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGt                                                                     <  2:2116899/93‑1 (MQ=255)

                    cGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGtt                                                                    <  2:4348956/93‑1 (MQ=255)

                     gggCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTa                                                                   >  2:2311645/1‑93 (MQ=255)

                      ggCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTaa                                                                  <  1:4373385/93‑1 (MQ=255)

                      ggCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTaa                                                                  <  2:4167043/93‑1 (MQ=255)

                        cAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGc                                                                >  2:3461860/1‑93 (MQ=255)

                        cAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGc                                                                <  1:663941/93‑1 (MQ=255)

                         aGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGTTGAAGCACTCAGCCCCGGCGTTAAGcc                                                               >  2:4497524/1‑93 (MQ=255)

                         aGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGcc                                                               >  2:2241431/1‑93 (MQ=255)

                         aGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGcc                                                               >  1:4673424/1‑93 (MQ=255)

                          gTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCt                                                              >  1:1624693/1‑93 (MQ=255)

                          gTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCt                                                              >  1:4045994/1‑93 (MQ=255)

                          gTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCt                                                              >  1:1077520/1‑93 (MQ=255)

                           tATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTc                                                             <  1:1473499/93‑1 (MQ=255)

                              ttATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCAtt                                                          >  2:3760465/1‑93 (MQ=255)

                              ttATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCAtt                                                          >  2:525182/1‑93 (MQ=255)

                               tATCGATAATATCCGCTACGCTGCAGCCCGCTTTGCGCCACCCGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTa                                                         <  2:4072703/93‑1 (MQ=255)

                                 tCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTAtc                                                       >  2:3612098/1‑93 (MQ=255)

                                   gATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTAtctc                                                     <  1:1774842/93‑1 (MQ=255)

                                     taataTCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCtctt                                                   >  1:4203932/1‑93 (MQ=255)

                                      aataTCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCtcttc                                                  <  1:3676072/93‑1 (MQ=255)

                                       atatCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCtcttct                                                 >  1:2518007/1‑93 (MQ=255)

                                       atatCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCtcttct                                                 <  2:314879/93‑1 (MQ=255)

                                       atatCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCtcttct                                                 <  2:148181/93‑1 (MQ=255)

                                        tatCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTtctc                                                >  1:1054804/1‑93 (MQ=255)

                                          tCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTcca                                              <  1:3197152/93‑1 (MQ=255)

                                          tCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTcca                                              <  1:3436259/93‑1 (MQ=255)

                                             gCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTccagcc                                           >  2:1254573/1‑93 (MQ=255)

                                             gCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTccagcc                                           <  1:3920141/93‑1 (MQ=255)

                                             gCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTccagcc                                           <  1:434978/93‑1 (MQ=255)

                                             gCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTT‑AGCCTCATTATCTCTTCTccagcca                                          <  2:2285539/93‑1 (MQ=255)

                                              cTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTccagcca                                          <  2:247909/93‑1 (MQ=255)

                                               tACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTccagccag                                         >  1:2461813/1‑93 (MQ=255)

                                               tACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTccagccag                                         >  2:2506737/1‑93 (MQ=255)

                                               tACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTccagccag                                         >  1:3204092/1‑93 (MQ=255)

                                               tACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTccagccag                                         >  1:2731422/1‑93 (MQ=255)

                                                aCGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGt                                        <  1:4009253/93‑1 (MQ=255)

                                                 cGCTGCCGACCGCTTTGCGCCACACGGCAAGCGGATATTAGTTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTa                                       <  2:1441413/93‑1 (MQ=255)

                                                 cGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTa                                       <  2:1875847/93‑1 (MQ=255)

                                                 cGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTa                                       <  2:2250769/93‑1 (MQ=255)

                                                 cGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTa                                       >  1:2583701/1‑93 (MQ=255)

                                                  gCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTAt                                      <  2:2326185/93‑1 (MQ=255)

                                                   cTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATc                                     >  2:2317057/1‑93 (MQ=255)

                                                   cTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATc                                     >  1:484854/1‑93 (MQ=255)

                                                    tGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCa                                    <  2:2051429/93‑1 (MQ=255)

                                                    tGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCa                                    >  2:3949473/1‑93 (MQ=255)

                                                    ctccGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCa                                    <  1:733785/91‑1 (MQ=255)

                                                       cGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGc                                 <  2:1938739/93‑1 (MQ=255)

                                                       cGACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTATCCAGCCAGTATCAGGc                                 <  1:744960/93‑1 (MQ=255)

                                                        gACCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCa                                >  1:335749/1‑93 (MQ=255)

                                                         aCCGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCAc                               >  1:4658478/1‑93 (MQ=255)

                                                           cGCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTg                             >  1:3934172/1‑93 (MQ=255)

                                                            gCTTTGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTgg                            >  2:404034/1‑93 (MQ=255)

                                                              tttGCGCCACACGGCAAGCGAATATTAGCTGAAGCCCTCAGCACCGGCGTTAAGCTGCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCt                          <  2:224319/93‑1 (MQ=255)

                                                              tttGCGCCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCt                          <  1:2138240/93‑1 (MQ=255)

                                                                 gcgcCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTAtt                       <  2:3286252/93‑1 (MQ=255)

                                                                 gcgcCACACGGCAAGCGAATATTAGCTAAAGCACTCAGCCCCGGAGTTAAGCATCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTAtt                       <  2:4353187/93‑1 (MQ=255)

                                                                   gcCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTAttgt                     <  2:1524477/93‑1 (MQ=255)

                                                                   gcCACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTAttgt                     <  2:2641478/93‑1 (MQ=255)

                                                                    ccACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTAttgtt                    >  2:2194222/1‑93 (MQ=255)

                                                                    ccACACGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTAttgtt                    <  2:2349821/93‑1 (MQ=255)

                                                                      acacGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGa                  >  2:1351724/1‑93 (MQ=255)

                                                                        ccGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAag                <  1:1389811/92‑1 (MQ=255)

                                                                         cGGCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAaga               <  2:672/93‑1 (MQ=255)

                                                                          ggCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAagag              >  2:1146707/1‑93 (MQ=255)

                                                                          ggCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAagag              <  1:274478/93‑1 (MQ=255)

                                                                          ggCAAGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAagag              >  2:4582061/1‑93 (MQ=255)

                                                                             aaGCGAATATTACCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGtt           <  2:1381307/93‑1 (MQ=255)

                                                                              aGCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTg          <  2:157547/93‑1 (MQ=255)

                                                                               gCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTgc         >  1:2934297/1‑93 (MQ=255)

                                                                               gCGAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTgc         <  1:1635417/93‑1 (MQ=255)

                                                                               gCGAATATAAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTgc         <  1:2689378/93‑1 (MQ=255)

                                                                                 gAATATTAGTTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTgcgc       >  2:4029656/1‑93 (MQ=255)

                                                                                 gAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTgcgc       <  2:3759184/93‑1 (MQ=255)

                                                                                 gAATATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTgcgc       <  1:3710245/93‑1 (MQ=255)

                                                                                  aaTATTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTGcgcg      >  2:2743599/1‑93 (MQ=255)

                                                                                   atatTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTGCGCga     <  2:2499667/93‑1 (MQ=255)

                                                                                     atTAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGCTGCGCgaga   >  1:3879911/1‑93 (MQ=255)

                                                                                      ttAGCTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTGCGCGAGAt  <  1:1357844/93‑1 (MQ=255)

GCGAAGATGCTGAACGGTACCGGGCAGTATTTATCGATAATATCCGCTACGCTGCCGACCGCTTTGCGCCACACGGCAAGCGAATATTAGTTGAAGCACTCAGCCCCGGCGTTAAGCCTCATTATCTCTTCTCCAGCCAGTATCAGGCACTGGCTATTGTTGAAGAGGTTGCGCGAGAT  >  NC_000913/2862593‑2862771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`