BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,877,392	T→C	E135G (GAG→GGG)	b2755 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,877,392	0	T	C	96.2%	476.0 / 5.7	160	E135G (GAG→GGG)	b2755	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (3/3); new base C (59/95); total (62/98)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 171 total aligned reads displayed.

TAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCAGAGC  >  NC_000913/2877302‑2877480

tAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGctct                                                                                        <  1:4035895/93‑1 (MQ=255)

tAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGccct                                                                                        <  2:1680779/93‑1 (MQ=255)

 aGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTa                                                                                       <  2:1444428/93‑1 (MQ=255)

 aGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTa                                                                                       <  1:3309437/93‑1 (MQ=255)

     tcGTCCATTCCATGTCACGCCGTATTGCTTCGCCATAAGTGCGCAGGTTGCCCTCACGCGACTGCCTTCTATATTTCTGAGTTGCCCTACgta                                                                                   >  2:4358500/2‑91 (MQ=255)

     aCGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGa                                                                                   <  2:1279212/93‑1 (MQ=255)

      cGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAg                                                                                  <  2:2316790/93‑1 (MQ=255)

       gTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAgc                                                                                 <  1:3458233/93‑1 (MQ=255)

        tccattccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAgcg                                                                                >  2:34017/1‑93 (MQ=255)

          cattccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGcc                                                                              <  1:1598848/93‑1 (MQ=255)

            ttccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCgg                                                                            >  2:3090093/1‑93 (MQ=255)

            ttccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCgg                                                                            <  1:3040352/93‑1 (MQ=255)

            ttccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCgg                                                                            <  2:2978943/93‑1 (MQ=255)

             tccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCggg                                                                           <  2:3321141/93‑1 (MQ=255)

             tccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCggg                                                                           >  1:1886339/1‑93 (MQ=255)

               catGTCACGCCGTCTTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCGGGCa                                                                         <  1:4328655/93‑1 (MQ=255)

                atGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAg                                                                        >  2:4309918/1‑93 (MQ=255)

                 tGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAgg                                                                       <  2:2437192/93‑1 (MQ=255)

                 tGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACATCTGAGTGGCCCTACGGAGCGCCCGGCAgg                                                                       >  2:4313789/1‑93 (MQ=255)

                  gTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGgc                                                                      >  2:4595715/1‑93 (MQ=255)

                   tCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGgcg                                                                     <  1:1397757/93‑1 (MQ=255)

                     aCGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCa                                                                   <  1:517238/93‑1 (MQ=255)

                     aCGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCa                                                                   >  2:4713469/1‑93 (MQ=255)

                      cGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAg                                                                  <  1:1673955/93‑1 (MQ=255)

                      cGCCGTATTGCGTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGTGCAGGCGCAg                                                                  <  1:2409059/93‑1 (MQ=255)

                       gcagTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAgg                                                                 <  2:4151980/90‑1 (MQ=255)

                        ccGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGt                                                                >  1:3702154/1‑93 (MQ=255)

                        ccGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGt                                                                <  2:3697114/93‑1 (MQ=255)

                          gTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTTGCCTACGGAGCGCCGGGCAGGCGCAGGCtc                                                              >  1:4148119/1‑93 (MQ=255)

                          gTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtc                                                              <  1:1452694/93‑1 (MQ=255)

                           tATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCGGGCAGGCGCAGGTtct                                                             >  2:4002809/1‑93 (MQ=255)

                           tATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtct                                                             >  1:2950/1‑93 (MQ=255)

                            aTTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtctc                                                            >  1:1237538/1‑93 (MQ=255)

                             ttGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTcc                                                           <  1:890115/93‑1 (MQ=255)

                                 ttCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAc                                                       >  1:3538035/1‑93 (MQ=255)

                                 ttCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAc                                                       <  1:4287527/93‑1 (MQ=255)

                                 ttCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAc                                                       <  2:210717/93‑1 (MQ=255)

                                  tCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAcc                                                      <  2:3515123/93‑1 (MQ=255)

                                     ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                   <  1:3772519/93‑1 (MQ=255)

                                     ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                   >  2:1854013/1‑93 (MQ=255)

                                     ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                   <  1:2571079/93‑1 (MQ=255)

                                     ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                   <  2:447887/93‑1 (MQ=255)

                                     ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                   <  1:2322702/93‑1 (MQ=255)

                                     ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                   >  2:28357/1‑93 (MQ=255)

                                     ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGGTCTCCAAACCGaa                                                   >  2:1854014/1‑93 (MQ=255)

                                      cAGAGGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATTCCTATGAGTTGCCCTCCGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAg                                                  <  2:685100/93‑1 (MQ=255)

                                      cAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAg                                                  <  2:3913245/93‑1 (MQ=255)

                                        gAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGtt                                                >  2:2596050/1‑93 (MQ=255)

                                         aaGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTc                                               <  1:2209455/93‑1 (MQ=255)

                                           gTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCaa                                             >  2:45020/1‑93 (MQ=255)

                                              cGTAGGTTGCCCGCACGCGACTGCCTTCTATGCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACa                                          <  2:4179491/93‑1 (MQ=255)

                                              cGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACa                                          <  1:1746523/93‑1 (MQ=255)

                                               gTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAt                                         <  2:4664368/93‑1 (MQ=255)

                                                 aGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAttt                                       <  1:1144873/93‑1 (MQ=255)

                                                  ggTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAtttt                                      >  1:1277177/1‑93 (MQ=255)

                                                    ttGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtac                                    <  2:1945509/93‑1 (MQ=255)

                                                    ttGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtac                                    <  1:2424257/93‑1 (MQ=255)

                                                      gCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgt                                  <  1:3430827/93‑1 (MQ=255)

                                                      gCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgt                                  <  1:1992710/93‑1 (MQ=255)

                                                       gccGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgta                                 <  2:841436/92‑1 (MQ=255)

                                                       cccGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAGCCGAAGTTCAAGCATTTtacgta                                 <  1:4642363/93‑1 (MQ=255)

                                                       cccGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgta                                 >  2:1480436/1‑93 (MQ=255)

                                                         cGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgtacg                               >  1:1529622/1‑93 (MQ=255)

                                                          gCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACGGAAGTTCAAACCTTTTACATACGa                              >  2:1201687/1‑93 (MQ=255)

                                                          gCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGa                              >  2:3794865/1‑93 (MQ=255)

                                                           cACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGAc                             >  1:799317/1‑93 (MQ=255)

                                                            ccgcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGAcc                            <  1:17995/92‑1 (MQ=255)

                                                             cgcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCACACCGAAGTTCAAACATTTTACGTACGACCt                           >  2:4095472/1‑93 (MQ=255)

                                                             cgcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCt                           >  1:2015910/1‑93 (MQ=255)

                                                             cgcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCt                           <  1:2451469/93‑1 (MQ=255)

                                                             cgcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCt                           <  1:1046101/93‑1 (MQ=255)

                                                             cgcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGCTCTCAAAACCGATGTTCAAACATTTTACGTACGACCt                           >  2:1121175/1‑93 (MQ=255)

                                                              gcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCtt                          <  2:4125706/93‑1 (MQ=255)

                                                              gcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCtt                          >  1:32654/1‑93 (MQ=255)

                                                               cgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTc                         <  1:3026379/93‑1 (MQ=255)

                                                                gACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCa                        <  1:756151/93‑1 (MQ=255)

                                                                gACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCa                        <  1:2307725/93‑1 (MQ=255)

                                                                gACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCa                        <  2:432683/93‑1 (MQ=255)

                                                                 aCTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAg                       <  2:1107708/93‑1 (MQ=255)

                                                                  cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                      <  1:2380135/93‑1 (MQ=255)

                                                                   tGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGAc                     >  1:4507965/1‑93 (MQ=255)

                                                                   tGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGAc                     <  1:890560/93‑1 (MQ=255)

                                                                    gCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACg                    <  2:447052/93‑1 (MQ=255)

                                                                        tCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCaaa                >  1:2477578/1‑93 (MQ=255)

                                                                         cTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAAt               >  2:724334/1‑93 (MQ=255)

                                                                         cTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAAt               <  1:2131070/93‑1 (MQ=255)

                                                                          tataCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATc              >  1:4620913/1‑93 (MQ=255)

                                                                          tataCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGGTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATc              >  2:3720442/1‑93 (MQ=255)

                                                                           ataCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCt             <  1:587016/93‑1 (MQ=255)

                                                                           ataCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCt             <  2:651171/93‑1 (MQ=255)

                                                                              ccTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCa          <  2:347484/93‑1 (MQ=255)

                                                                              ccTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCa          >  1:107253/1‑93 (MQ=255)

                                                                               ctctGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCAt         <  2:3138126/93‑1 (MQ=255)

                                                                               ctctGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCAt         <  2:3138127/93‑1 (MQ=255)

                                                                               ctctGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGGTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCAt         >  2:3318088/1‑93 (MQ=255)

                                                                                  tGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCa      >  2:3972340/1‑93 (MQ=255)

                                                                                  tGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCa      <  2:835748/93‑1 (MQ=255)

                                                                                     gTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCagag   >  1:51652/1‑93 (MQ=255)

                                                                                     gTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCagag   >  2:1165526/1‑93 (MQ=255)

                                                                                      ttGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTCCGACCTTCAGACGCAAATCTTCATCCAGAGc  <  1:2496221/93‑1 (MQ=255)

TAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCAGAGC  >  NC_000913/2877302‑2877480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`