BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,915,759	A→G	D894G (GAT→GGT)	barA →	sensor‑regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,915,759	0	A	G	99.4%	507.8 / ‑5.2	155	D894G (GAT→GGT)	barA	sensor‑regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (89/65); minor base T (0/1); total (89/66)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 180 total aligned reads displayed.

ATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTC  >  NC_000913/2915669‑2915848

attgCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGtt                                                                                         >  1:2319806/4‑93 (MQ=255)

 tGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGttt                                                                                        >  1:3786822/1‑93 (MQ=255)

  ggCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTg                                                                                       <  2:2508122/93‑1 (MQ=255)

  ggCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTg                                                                                       <  1:1917500/93‑1 (MQ=255)

    cAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGa                                                                                     <  1:4160745/93‑1 (MQ=255)

     aGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGaa                                                                                    <  1:3383005/93‑1 (MQ=255)

      gTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAAc                                                                                   >  2:3516565/1‑93 (MQ=255)

         gCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCgg                                                                                >  2:3237263/1‑93 (MQ=255)

          cGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGa                                                                               >  1:2270170/1‑93 (MQ=255)

          cGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGa                                                                               <  1:3578450/93‑1 (MQ=255)

           ggCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAg                                                                              >  1:4303711/1‑93 (MQ=255)

           ggCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAg                                                                              <  2:2566636/93‑1 (MQ=255)

            gCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgc                                                                             <  2:3298321/93‑1 (MQ=255)

             cTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgct                                                                            <  1:4445739/93‑1 (MQ=255)

             cTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgct                                                                            <  1:3188834/93‑1 (MQ=255)

               ataGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgctgc                                                                          >  2:2203007/1‑93 (MQ=255)

                taGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgctgct                                                                         >  2:2369071/1‑93 (MQ=255)

                 aGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGctgctg                                                                        >  2:245923/1‑93 (MQ=255)

                    ggTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGaa                                                                     >  2:2645969/1‑93 (MQ=255)

                     gtgtGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAAc                                                                    <  1:280194/93‑1 (MQ=255)

                       gtgCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTg                                                                  >  2:4308255/1‑93 (MQ=255)

                       gtgCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTg                                                                  >  1:4214033/1‑93 (MQ=255)

                        tgCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGt                                                                 <  1:216798/93‑1 (MQ=255)

                         gCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGtt                                                                >  1:1813490/1‑93 (MQ=255)

                         gCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGtt                                                                <  1:1299851/93‑1 (MQ=255)

                         gCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGtt                                                                <  1:3038780/93‑1 (MQ=255)

                         gCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGtt                                                                >  2:422526/1‑93 (MQ=255)

                          ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTg                                                               <  1:3564532/93‑1 (MQ=255)

                            aCGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGa                                                             >  1:2577458/1‑93 (MQ=255)

                            aCGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGa                                                             >  1:4560758/1‑93 (MQ=255)

                             cGTATGAAGAATCTCTGCCACCTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGATCTGCTGGAACTGTTGTAc                                                            <  2:3317088/93‑1 (MQ=255)

                                 tGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACgaga                                                        >  2:4525807/1‑93 (MQ=255)

                                  gaagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGAt                                                       <  2:1875380/93‑1 (MQ=255)

                                   aagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATg                                                      <  1:2814707/93‑1 (MQ=255)

                                   aagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATg                                                      <  2:4025166/93‑1 (MQ=255)

                                    agaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATgg                                                     >  2:666776/1‑93 (MQ=255)

                                     gaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGa                                                    <  2:2870145/93‑1 (MQ=255)

                                     gaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGa                                                    >  2:2646711/1‑93 (MQ=255)

                                      aaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCTGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                                                   <  1:74079/93‑1 (MQ=255)

                                      aaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                                                   <  2:3216572/93‑1 (MQ=255)

                                       aTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGCGATGGATa                                                  >  1:2250914/1‑93 (MQ=255)

                                       aTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATa                                                  >  2:3805115/1‑93 (MQ=255)

                                       aTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATa                                                  >  1:132550/1‑93 (MQ=255)

                                       aTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATa                                                  >  2:2989255/1‑93 (MQ=255)

                                         ctctGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAAt                                                <  1:204234/93‑1 (MQ=255)

                                         ctctGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAAt                                                >  1:3206203/1‑93 (MQ=255)

                                            tGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTc                                             <  2:2669931/93‑1 (MQ=255)

                                              ccAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgc                                           >  1:3244406/1‑93 (MQ=255)

                                                aaCTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgc                                         >  1:3093962/1‑93 (MQ=255)

                                                aaCTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgc                                         <  2:164499/93‑1 (MQ=255)

                                                 aCTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgcg                                        >  1:1595122/1‑93 (MQ=255)

                                                 aCTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgcg                                        >  2:2488269/1‑93 (MQ=255)

                                                 aCTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgcg                                        <  2:4588093/93‑1 (MQ=255)

                                                  cTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgcgc                                       <  1:1177372/93‑1 (MQ=255)

                                                   tGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgcgcg                                      <  1:653715/93‑1 (MQ=255)

                                                   tGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgcgcg                                      >  2:1072490/1‑93 (MQ=255)

                                                    gATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGa                                     >  2:1530872/1‑93 (MQ=255)

                                                      tCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAg                                   >  1:4281798/1‑93 (MQ=255)

                                                        gagcaacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGcc                                 <  2:1294271/90‑1 (MQ=255)

                                                        gAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGcc                                 >  2:151868/1‑93 (MQ=255)

                                                          acaacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAGCCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAg                               <  2:560739/93‑1 (MQ=255)

                                                          acaacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAg                               >  1:833478/1‑93 (MQ=255)

                                                           caacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGc                              >  1:3722469/1‑93 (MQ=255)

                                                           caacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGc                              >  1:1627305/1‑93 (MQ=255)

                                                            aacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCa                             >  2:1982398/1‑93 (MQ=255)

                                                             acaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCaa                            >  1:1134384/1‑93 (MQ=255)

                                                             acaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCaa                            >  2:2870937/1‑93 (MQ=255)

                                                             acaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCaa                            >  1:4678469/1‑93 (MQ=255)

                                                              caGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCaaa                           <  2:2304733/93‑1 (MQ=255)

                                                              caGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCaaa                           >  2:2132095/1‑93 (MQ=255)

                                                                  tACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtc                       <  2:230051/93‑1 (MQ=255)

                                                                  tACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtc                       >  1:2560628/1‑93 (MQ=255)

                                                                  tACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtc                       >  1:988369/1‑93 (MQ=255)

                                                                  tACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtc                       >  2:2960646/1‑93 (MQ=255)

                                                                   aCGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtct                      >  1:4429148/1‑93 (MQ=255)

                                                                   aCGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtct                      >  1:312436/1‑93 (MQ=255)

                                                                    cGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtctc                     <  1:4138346/93‑1 (MQ=255)

                                                                      tAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCgg                   <  1:1039663/93‑1 (MQ=255)

                                                                      tAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCgg                   <  1:2260531/93‑1 (MQ=255)

                                                                      tAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCgg                   >  1:1025604/1‑93 (MQ=255)

                                                                        gCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGt                 >  1:3267778/1‑93 (MQ=255)

                                                                         cGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTa                <  1:1872541/93‑1 (MQ=255)

                                                                          gggtcTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTaa               <  1:1392901/89‑1 (MQ=255)

                                                                           gTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAAt              >  2:641452/1‑93 (MQ=255)

                                                                              cTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGa           <  1:1527266/93‑1 (MQ=255)

                                                                                aaaGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATg         >  2:3705065/1‑93 (MQ=255)

                                                                                 aagaagaagGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGt        >  2:3093103/1‑93 (MQ=255)

                                                                                  agaagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTc       >  1:3330111/1‑93 (MQ=255)

                                                                                  agaagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTc       <  2:3727968/93‑1 (MQ=255)

                                                                                  agaagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTc       >  2:3926240/1‑93 (MQ=255)

                                                                                  agaagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTc       <  1:549069/93‑1 (MQ=255)

                                                                                    aagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCtt     <  2:1326783/93‑1 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCGGCAAAATTCTCGGGTAATGGATGTCTTg    <  1:2748223/93‑1 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCttt    >  1:2932130/1‑92 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTg    >  2:4019126/1‑93 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTg    >  2:4664583/1‑93 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTg    <  2:1743191/93‑1 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTg    >  1:2932129/1‑93 (MQ=255)

                                                                                      gaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGt   >  1:2250154/1‑93 (MQ=255)

                                                                                       aaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTc  <  2:3885000/93‑1 (MQ=255)

ATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTC  >  NC_000913/2915669‑2915848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`