BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,925,058	A→C	E243D (GAA→GAC)	ygdH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,925,058	0	A	C	98.3%	588.4 / ‑1.6	181	E243D (GAA→GAC)	ygdH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/1); new base C (93/85); total (95/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

Only 100 of 189 total aligned reads displayed.

TATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAATTTTAAT  >  NC_000913/2924968‑2925147

tATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGc                                                                                         >  2:2081825/1‑93 (MQ=255)

  tGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGt                                                                                       >  2:4239093/1‑93 (MQ=255)

  tGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCCGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGt                                                                                       >  1:385588/1‑93 (MQ=255)

   gACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGtt                                                                                      >  2:3111009/1‑93 (MQ=255)

   gACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGtt                                                                                      <  2:2041518/93‑1 (MQ=255)

   gACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGtt                                                                                      <  1:1449734/93‑1 (MQ=255)

     cAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTg                                                                                    >  1:1550668/1‑93 (MQ=255)

      agagCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGt                                                                                   >  2:2219061/1‑93 (MQ=255)

       gagCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTc                                                                                  <  2:3392399/93‑1 (MQ=255)

       gagCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTc                                                                                  >  1:796416/1‑93 (MQ=255)

         gCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCg                                                                                >  2:62760/1‑93 (MQ=255)

         gCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCg                                                                                <  1:2862778/93‑1 (MQ=255)

           cGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTa                                                                              <  2:2073057/93‑1 (MQ=255)

            gTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTAt                                                                             >  1:2236777/1‑93 (MQ=255)

            gTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTAt                                                                             >  2:1323136/1‑93 (MQ=255)

            gTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAAAGTCTGGACGCGTTTGTCCGTAt                                                                             >  1:3493900/1‑93 (MQ=255)

             tCGGTTATCGCCGCTGAACCGCCTAACCCGCTGGGCAACGAATTGATCCTCATGCCAGGTATCGAAAAACGTCTGGACGAGTTTGTCCGTATc                                                                            >  2:3026322/1‑93 (MQ=255)

             tCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATc                                                                            <  2:2536183/93‑1 (MQ=255)

             tCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATc                                                                            <  1:1999727/93‑1 (MQ=255)

                aTTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCt                                                                         >  1:143902/1‑93 (MQ=255)

                aTTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCt                                                                         >  2:1528383/1‑93 (MQ=255)

                aTTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCt                                                                         <  2:759790/93‑1 (MQ=255)

                 ttATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTc                                                                        >  1:3334525/1‑93 (MQ=255)

                   aTCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCAc                                                                      >  1:3415816/1‑93 (MQ=255)

                    tCGCCGCTGACCCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACg                                                                     <  2:4287869/93‑1 (MQ=255)

                    tCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACg                                                                     <  1:2661856/93‑1 (MQ=255)

                    tCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACg                                                                     >  2:2162785/1‑93 (MQ=255)

                      gccgcTGACCCGCCTCACCCGCTGGTCAACGAATTGATCATCATGCCTGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGt                                                                   <  2:4224721/93‑1 (MQ=255)

                      gccgcTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGt                                                                   >  1:4675451/1‑93 (MQ=255)

                      gccgcTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGt                                                                   >  2:1903257/1‑93 (MQ=255)

                          cTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCa                                                               <  1:996795/93‑1 (MQ=255)

                           tGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCAt                                                              >  1:2545837/1‑93 (MQ=255)

                           tGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCAt                                                              >  2:3318830/1‑93 (MQ=255)

                           tGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCAt                                                              <  1:1793850/93‑1 (MQ=255)

                           tGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCAt                                                              >  1:862995/1‑93 (MQ=255)

                           tGAACCGCCTAACCCGCCGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCAt                                                              >  2:1543247/1‑93 (MQ=255)

                            gAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCAtt                                                             >  2:2352699/1‑93 (MQ=255)

                            gAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCAtt                                                             >  1:1240891/1‑93 (MQ=255)

                             aaCCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTa                                                            <  1:3052955/93‑1 (MQ=255)

                               ccGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATc                                                          >  1:1354668/1‑93 (MQ=255)

                                cGCCTAACCCGCTGGTCAACGAATGGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCt                                                         <  1:3222568/93‑1 (MQ=255)

                                 gCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCtt                                                        >  1:1007996/1‑93 (MQ=255)

                                 gCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCtt                                                        <  1:2258078/93‑1 (MQ=255)

                                   cTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTcc                                                      >  1:3982911/1‑93 (MQ=255)

                                   cTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTcc                                                      >  2:496933/1‑93 (MQ=255)

                                    tAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTccc                                                     <  1:2712998/93‑1 (MQ=255)

                                    tAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTccc                                                     <  2:3031639/93‑1 (MQ=255)

                                     aaCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCt                                                    >  2:368000/1‑93 (MQ=255)

                                     aaCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCt                                                    >  2:113810/1‑93 (MQ=255)

                                     aaCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCt                                                    >  1:1946787/1‑93 (MQ=255)

                                     aaCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCt                                                    <  2:820011/93‑1 (MQ=255)

                                     aaCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCt                                                    <  1:315151/93‑1 (MQ=255)

                                     aaCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCt                                                    <  1:4336779/93‑1 (MQ=255)

                                      aCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACTGTATCATTATCTTCCCTg                                                   >  2:1830634/1‑93 (MQ=255)

                                      aCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTg                                                   >  2:111231/1‑93 (MQ=255)

                                      aCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTg                                                   <  2:1673779/93‑1 (MQ=255)

                                      aCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTg                                                   >  1:2401356/1‑93 (MQ=255)

                                      aCCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTg                                                   >  2:437924/1‑93 (MQ=255)

                                         cGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCg                                                >  1:4618090/1‑93 (MQ=255)

                                          gCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCgg                                               <  2:4575387/93‑1 (MQ=255)

                                          gCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCgg                                               <  1:3573241/93‑1 (MQ=255)

                                           cTGGTCAACGAATTGATCATCACGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGgt                                              <  2:2701735/93‑1 (MQ=255)

                                            tGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGgtg                                             <  1:2580990/93‑1 (MQ=255)

                                             ggTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGgtgt                                            <  2:514499/93‑1 (MQ=255)

                                                cAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTggg                                         <  2:1395941/93‑1 (MQ=255)

                                                 caccaaTTGATCATCATGCCAGATATCGAAAATCGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGt                                        <  1:1036591/89‑1 (MQ=255)

                                                  aCGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTa                                       >  1:2756441/1‑93 (MQ=255)

                                                   cGAATCGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTAc                                      <  2:937616/93‑1 (MQ=255)

                                                    gAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACg                                     >  2:1958565/1‑93 (MQ=255)

                                                    gAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACg                                     <  1:3916827/93‑1 (MQ=255)

                                                    gAATTGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACg                                     >  1:4235512/1‑93 (MQ=255)

                                                    caaTTGATCATCATGCCAGATATCCAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACg                                     <  2:3908271/92‑1 (MQ=255)

                                                       ttGATCATCATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCa                                  <  1:913832/93‑1 (MQ=255)

                                                       ttGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCTTGGCGGTGTGGGGACGGCa                                  >  2:4270565/1‑93 (MQ=255)

                                                          atcatcATGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCagaa                               <  2:3528311/93‑1 (MQ=255)

                                                           tcatcaTGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCagaag                              >  2:926502/1‑93 (MQ=255)

                                                           tcatcaTGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCagaag                              <  2:3627719/93‑1 (MQ=255)

                                                             atcatGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAagag                            >  2:2287960/1‑93 (MQ=255)

                                                              tcatGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGt                           <  1:3552235/93‑1 (MQ=255)

                                                               catGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGtt                          >  1:3937313/1‑93 (MQ=255)

                                                                 tGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGc                        <  1:2296087/93‑1 (MQ=255)

                                                                 tGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGc                        <  1:3807427/93‑1 (MQ=255)

                                                                 tGCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGc                        <  1:3309017/93‑1 (MQ=255)

                                                                  gCCAGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGct                       <  1:3612208/93‑1 (MQ=255)

                                                                    cAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGctct                     >  1:4035761/1‑93 (MQ=255)

                                                                     aGATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTa                    >  2:3769715/1‑93 (MQ=255)

                                                                      gATATCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTAt                   >  2:3945483/1‑93 (MQ=255)

                                                                       atatCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTAtt                  <  1:1856174/93‑1 (MQ=255)

                                                                        tatCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTAttt                 >  1:4552045/1‑93 (MQ=255)

                                                                        tatCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTAttt                 >  1:4617121/1‑93 (MQ=255)

                                                                         atCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTg                >  1:3701361/1‑93 (MQ=255)

                                                                          tCGAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGc               <  2:2472532/93‑1 (MQ=255)

                                                                            gAAAAACGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTg             >  2:4469905/1‑93 (MQ=255)

                                                                             aaaaaCGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTgg            >  2:626334/1‑93 (MQ=255)

                                                                                aaCGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGaa         <  1:3000224/93‑1 (MQ=255)

                                                                                aaCGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGaa         >  2:387333/1‑93 (MQ=255)

                                                                                  cGTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAAtt       <  1:2940140/93‑1 (MQ=255)

                                                                                   gTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAAttt      >  2:3297934/1‑93 (MQ=255)

                                                                                   gTCTGGACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAAttt      >  1:4488031/1‑93 (MQ=255)

                                                                                       ggACGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAATTTTAAt  <  1:1113772/93‑1 (MQ=255)

TATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAATTTTAAT  >  NC_000913/2924968‑2925147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`