BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,864	T→C	M169T (ATG→ACG)	b2810 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,864	0	T	C	98.6%	442.0 / ‑2.5	139	M169T (ATG→ACG)	b2810	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (61/76); total (62/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 146 total aligned reads displayed.

AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCT  >  NC_000913/2941774‑2941952

aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                        <  2:1121785/93‑1 (MQ=255)

aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                        >  2:1380193/1‑93 (MQ=255)

aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                        >  1:1511526/1‑93 (MQ=255)

    gCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGaa                                                                                    >  1:145480/1‑93 (MQ=255)

    gCCGCTTAATGCGCAGCGACTGCCGGATGNCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGaa                                                                                    <  2:2883327/93‑1 (MQ=255)

    gCCGCTTAAAGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGaa                                                                                    <  2:137168/93‑1 (MQ=255)

       gCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGt                                                                                 >  2:4595198/1‑93 (MQ=255)

        cTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGACCGtt                                                                                >  2:3375922/1‑93 (MQ=255)

         ttAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTa                                                                               >  1:2779960/1‑93 (MQ=255)

         ttAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTa                                                                               <  1:3191548/93‑1 (MQ=255)

          tAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTAc                                                                              >  1:1816820/1‑93 (MQ=255)

           taTGCGCAGCGGCTGCCGGATGTCGATTTGTTGCCAGAACTGATTACCCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACt                                                                             <  2:2665154/92‑1 (MQ=255)

           aaTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACt                                                                             <  1:3719761/93‑1 (MQ=255)

           aaTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACt                                                                             <  2:3732833/93‑1 (MQ=255)

           aaTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACt                                                                             >  2:1818740/1‑93 (MQ=255)

            aTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTg                                                                            <  2:3312208/93‑1 (MQ=255)

                gcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgg                                                                        >  2:3122361/1‑93 (MQ=255)

                 cAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGt                                                                       <  2:66725/93‑1 (MQ=255)

                 cAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGt                                                                       >  2:1943416/1‑93 (MQ=255)

                  ggCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                      <  2:961449/92‑1 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                      >  2:1806842/1‑93 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                      <  2:4659123/93‑1 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                      <  1:787618/93‑1 (MQ=255)

                      aCTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGccc                                                                  >  2:3494442/1‑93 (MQ=255)

                       cTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCg                                                                 >  2:3691889/1‑93 (MQ=255)

                       cTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCg                                                                 <  1:1673531/93‑1 (MQ=255)

                         gCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGa                                                               >  2:3111586/1‑93 (MQ=255)

                         gCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGa                                                               <  1:1391536/93‑1 (MQ=255)

                           cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTAAGACGTCGAACGCTACTGGCGGTTGCCCGGATc                                                             >  1:3002899/1‑93 (MQ=255)

                            ggATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCt                                                            >  1:1110543/1‑93 (MQ=255)

                            ggATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCt                                                            <  2:3317699/93‑1 (MQ=255)

                             gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTg                                                           <  2:3752494/93‑1 (MQ=255)

                             gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTg                                                           <  1:722473/93‑1 (MQ=255)

                             gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTg                                                           <  2:1898316/93‑1 (MQ=255)

                              aTGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTgg                                                          <  2:4089209/93‑1 (MQ=255)

                              aTGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTgg                                                          <  2:600989/93‑1 (MQ=255)

                              aTGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTgg                                                          >  2:1124304/1‑93 (MQ=255)

                              aTGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTgg                                                          <  2:826439/93‑1 (MQ=255)

                               tGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTGGAACGTTACTGGCGGTTGCCCCGGTCTGgc                                                         >  1:1039135/1‑93 (MQ=255)

                               tGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTggg                                                         >  1:1127537/1‑92 (MQ=255)

                               tGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTggg                                                         >  1:3527844/1‑92 (MQ=255)

                                gTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGgcg                                                        >  2:3304995/1‑93 (MQ=255)

                                gTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGgcg                                                        >  1:618634/1‑93 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTGCTCCCCGTAGTCGGATTCTGGCGTTGGGTAAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                     <  2:1463951/93‑1 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGcgcgg                                                     >  2:1445533/1‑92 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                     <  2:4326118/93‑1 (MQ=255)

                                     tttGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagc                                                   >  1:4670284/1‑93 (MQ=255)

                                     tttGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagc                                                   >  2:1040841/1‑93 (MQ=255)

                                       tgttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagcga                                                 <  2:4568609/93‑1 (MQ=255)

                                        gttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAt                                                >  2:2366610/1‑93 (MQ=255)

                                        gttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAt                                                <  2:2657495/93‑1 (MQ=255)

                                        gttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAt                                                <  2:4710787/93‑1 (MQ=255)

                                        gttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAt                                                <  1:3392091/93‑1 (MQ=255)

                                         ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                               <  2:3198499/93‑1 (MQ=255)

                                         ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                               <  1:1919121/93‑1 (MQ=255)

                                         ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                               <  2:3198500/93‑1 (MQ=255)

                                          tgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTa                                              <  2:900867/93‑1 (MQ=255)

                                            ccAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAcc                                            <  1:1727256/93‑1 (MQ=255)

                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                           <  1:4175696/93‑1 (MQ=255)

                                               gAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGTATCTGGCGCGGGCGATTACCctt                                         >  2:2608536/1‑93 (MQ=255)

                                               gAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCttt                                         <  2:1742617/93‑1 (MQ=255)

                                               gAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCttt                                         <  2:398301/93‑1 (MQ=255)

                                               gAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCttt                                         >  1:2332162/1‑93 (MQ=255)

                                                 aCTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGc                                       >  1:1930116/1‑93 (MQ=255)

                                                 aCTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGc                                       >  1:3804556/1‑93 (MQ=255)

                                                    gATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCa                                    >  2:2191065/1‑93 (MQ=255)

                                                      ttACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCGATTACCTTTGCTCAtt                                  >  2:1063050/1‑93 (MQ=255)

                                                      ttACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCCtt                                  >  2:3395757/1‑93 (MQ=255)

                                                       tACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTc                                 <  2:1185650/93‑1 (MQ=255)

                                                        aCTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCa                                <  2:276173/93‑1 (MQ=255)

                                                         cTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAg                               >  1:3571641/1‑93 (MQ=255)

                                                           ccccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTCCCTTTGCTCATTCAGcc                             >  2:3483596/1‑93 (MQ=255)

                                                           ccccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGcc                             <  1:4381122/93‑1 (MQ=255)

                                                            cccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCg                            <  2:4265684/93‑1 (MQ=255)

                                                             ccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgg                           <  1:61530/93‑1 (MQ=255)

                                                             ccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgg                           <  2:1963657/93‑1 (MQ=255)

                                                             ccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgg                           <  1:4050513/93‑1 (MQ=255)

                                                                 aGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtg                       >  2:2741151/1‑93 (MQ=255)

                                                                 aGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtg                       >  2:834535/1‑93 (MQ=255)

                                                                  gTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTCCTTGCGGTTGCCCGGATCTGGCGCGTGCGATTACCTTTGCACATTCTGCCGGGAtgg                      >  2:4605955/1‑93 (MQ=255)

                                                                     ggATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtgg                   >  1:2320701/1‑93 (MQ=255)

                                                                     ggATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtgg                   <  1:4302728/93‑1 (MQ=255)

                                                                     ggATTCTGGCGTTCGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtgg                   >  1:3135659/1‑93 (MQ=255)

                                                                      gATTCTGGCGTTGGGTCAGGCGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATggtggt                  <  2:1607495/93‑1 (MQ=255)

                                                                       aTTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGgtggtg                 >  1:1828316/1‑93 (MQ=255)

                                                                       aTTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGCCCGGATCTGGGGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGggggtg                 >  1:3496438/1‑93 (MQ=255)

                                                                        ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGgga                >  2:2561923/1‑93 (MQ=255)

                                                                         tCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgat               <  2:2230081/93‑1 (MQ=255)

                                                                           tGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatgg             <  1:3140342/93‑1 (MQ=255)

                                                                             gCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggtt           >  1:3128850/1‑93 (MQ=255)

                                                                             gCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggtt           <  2:2146274/93‑1 (MQ=255)

                                                                              gtttGGGTCAGCCGTCCAACGTTACTGGCGGTTGCCCGGGTCTGTCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttg          <  1:6208/91‑1 (MQ=255)

                                                                               gTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttga         <  2:3652492/93‑1 (MQ=255)

                                                                               gTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttga         <  1:4347405/93‑1 (MQ=255)

                                                                                ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat        >  1:1411864/1‑93 (MQ=255)

                                                                                ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat        <  2:1269290/93‑1 (MQ=255)

                                                                                  gggTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgatgg      <  1:1969825/93‑1 (MQ=255)

                                                                                   ggTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgatggt     <  2:2829392/93‑1 (MQ=255)

                                                                                   ggTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgatggt     >  1:4680927/1‑93 (MQ=255)

                                                                                      cAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCt  <  2:2146096/93‑1 (MQ=255)

AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCT  >  NC_000913/2941774‑2941952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`