BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,854	T→C	L271P (CTG→CCG)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,854	0	T	C	98.5%	415.8 / ‑2.4	134	L271P (CTG→CCG)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (71/61); total (72/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 140 total aligned reads displayed.

CGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTC  >  NC_000913/2977764‑2977943

cGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGa                                                                                         >  1:695524/1‑93 (MQ=255)

 ggtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCAGTTCCGCTCACCGTCAGCCCGAt                                                                                        >  1:3716784/1‑93 (MQ=255)

  gtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATc                                                                                       >  1:19994/1‑93 (MQ=255)

   tggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATcc                                                                                      <  2:72331/93‑1 (MQ=255)

   tggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATcc                                                                                      <  2:4523257/93‑1 (MQ=255)

    ggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCg                                                                                     <  2:3859126/93‑1 (MQ=255)

     gtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGc                                                                                    >  1:868263/1‑93 (MQ=255)

       tAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGccc                                                                                  >  1:2887952/1‑93 (MQ=255)

       tAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTACGGTTCCGTTCACCGTCAGCCCGATGCGccc                                                                                  >  1:193918/1‑93 (MQ=255)

        aaCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGcccc                                                                                 >  1:1044030/1‑93 (MQ=255)

        aaCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGcccc                                                                                 >  1:868853/1‑93 (MQ=255)

         aCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGccccc                                                                                >  1:1582657/1‑93 (MQ=255)

         aCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGccccc                                                                                <  2:193639/93‑1 (MQ=255)

         aCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGccccc                                                                                <  1:467325/93‑1 (MQ=255)

         aCCCGCCCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTCCACCGTCAGCCCGATCCGccccc                                                                                <  2:3202943/93‑1 (MQ=255)

          cccGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCt                                                                               <  2:4554213/93‑1 (MQ=255)

            cGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGc                                                                             <  2:1604417/93‑1 (MQ=255)

             gCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCa                                                                            >  2:2476796/1‑93 (MQ=255)

              cTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAc                                                                           <  1:807615/93‑1 (MQ=255)

                 aCCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGc                                                                        >  2:1350107/1‑93 (MQ=255)

                 aCCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGc                                                                        >  2:1350106/1‑93 (MQ=255)

                     cACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCCGCCCGATCCGCCCCCTGCACCGCCCGt                                                                    >  2:1722810/1‑93 (MQ=255)

                     cACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGt                                                                    <  1:2432421/93‑1 (MQ=255)

                       cTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtca                                                                  >  1:1624293/1‑93 (MQ=255)

                         aGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcatc                                                                >  2:3902152/1‑93 (MQ=255)

                         aGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcatc                                                                >  1:2487981/1‑93 (MQ=255)

                            ttATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgc                                                             >  1:3938937/1‑93 (MQ=255)

                            ttATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgc                                                             >  1:3678400/1‑93 (MQ=255)

                              aTGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcgc                                                           <  1:1011380/93‑1 (MQ=255)

                              aTGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcgc                                                           <  1:3671547/93‑1 (MQ=255)

                               tGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCt                                                          <  1:4391177/93‑1 (MQ=255)

                                 cGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTgg                                                        <  1:2549941/93‑1 (MQ=255)

                                 cGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTgg                                                        <  2:131866/93‑1 (MQ=255)

                                  ggCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGt                                                       <  2:233594/93‑1 (MQ=255)

                                  ggCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGt                                                       <  1:4719631/93‑1 (MQ=255)

                                    cAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTc                                                     >  2:4294133/1‑93 (MQ=255)

                                     aaGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCa                                                    <  2:1905507/93‑1 (MQ=255)

                                     aaGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCa                                                    >  2:3824022/1‑93 (MQ=255)

                                     aaGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCa                                                    <  1:3297918/93‑1 (MQ=255)

                                      aGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAg                                                   >  2:601794/1‑93 (MQ=255)

                                      aGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAg                                                   <  2:3985533/93‑1 (MQ=255)

                                       gCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAgg                                                  >  1:2923740/1‑93 (MQ=255)

                                        cGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGc                                                 <  2:1366092/93‑1 (MQ=255)

                                         ggTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCg                                                >  2:1738699/1‑93 (MQ=255)

                                         ggTTTAGTGGTGCGGAGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCCGGTTCAGGCg                                                >  2:1916937/1‑93 (MQ=255)

                                          gTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGt                                               <  2:3497159/93‑1 (MQ=255)

                                          gTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGt                                               >  1:182127/1‑93 (MQ=255)

                                           tttAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGtt                                              <  2:2381599/93‑1 (MQ=255)

                                              aGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAg                                           >  1:1135528/1‑93 (MQ=255)

                                              aGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGGTCAGGCGTTTAg                                           >  2:1600414/1‑93 (MQ=255)

                                               gtggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGt                                          >  1:3040832/1‑93 (MQ=255)

                                                tggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTg                                         <  2:2502890/93‑1 (MQ=255)

                                                tggggCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTg                                         <  2:4736533/93‑1 (MQ=255)

                                                 ggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTgg                                        >  2:3189371/1‑93 (MQ=255)

                                                  gtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTggg                                       <  2:4711819/93‑1 (MQ=255)

                                                  gtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTggg                                       <  1:1315216/93‑1 (MQ=255)

                                                   tgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGc                                      <  1:1468546/93‑1 (MQ=255)

                                                     cggcggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCAt                                    >  2:4174903/1‑93 (MQ=255)

                                                       gcggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCAttt                                  >  2:2612885/1‑93 (MQ=255)

                                                        cggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTa                                 <  2:4296026/93‑1 (MQ=255)

                                                          gTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACa                               >  2:2459129/1‑93 (MQ=255)

                                                          gTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACa                               >  2:2459135/1‑93 (MQ=255)

                                                           ttCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACaa                              >  1:4076456/1‑93 (MQ=255)

                                                           ttCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACaa                              >  2:4747303/1‑93 (MQ=255)

                                                           ttCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACaa                              >  2:558639/1‑93 (MQ=255)

                                                           ttCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACaa                              >  1:2817999/1‑93 (MQ=255)

                                                           ttCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACaa                              >  1:2750035/1‑93 (MQ=255)

                                                           ttCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACaa                              <  2:612905/93‑1 (MQ=255)

                                                           ttCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCAGACCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACaa                              >  1:1501312/1‑93 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAg                             <  2:3028552/93‑1 (MQ=255)

                                                             cAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGc                            <  1:3800978/93‑1 (MQ=255)

                                                             cAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGc                            <  1:3752526/93‑1 (MQ=255)

                                                             cAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGc                            >  2:3194832/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          >  2:1210677/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          >  1:4577678/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          >  2:250092/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCAGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          >  2:949787/1‑93 (MQ=255)

                                                                tATTGCGGTTCCGTTCGCCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACACGCggg                         <  1:4016320/93‑1 (MQ=255)

                                                                 aTTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCCCCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgggg                        <  1:3623931/93‑1 (MQ=255)

                                                                 aTTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgggg                        >  1:3590373/1‑93 (MQ=255)

                                                                 aTTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgggg                        >  1:3957863/1‑93 (MQ=255)

                                                                   tGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGtt                      <  2:33235/93‑1 (MQ=255)

                                                                   tGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGtt                      <  1:1776070/93‑1 (MQ=255)

                                                                   tGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGCCATTTACAAGCGGGGtt                      <  2:3088350/93‑1 (MQ=255)

                                                                    gCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTa                     <  2:2544186/93‑1 (MQ=255)

                                                                       gTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCg                  >  2:1654923/1‑93 (MQ=255)

                                                                         tCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGaa                >  1:3767241/1‑93 (MQ=255)

                                                                          ccGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGaaa               >  2:2216267/1‑93 (MQ=255)

                                                                           cGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAAc              <  1:3280691/93‑1 (MQ=255)

                                                                           cGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAAc              <  2:873048/93‑1 (MQ=255)

                                                                           cGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAAc              <  1:1027406/93‑1 (MQ=255)

                                                                             ttCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTg            >  1:1390743/1‑93 (MQ=255)

                                                                                aCCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTc         <  1:994158/93‑1 (MQ=255)

                                                                                 ccGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCa        <  1:356632/93‑1 (MQ=255)

                                                                                   gTCAGCCCGATCCGCCCCCTGCACCGCCCATCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACt      >  2:2848574/1‑93 (MQ=255)

                                                                                   gTCAGCCCGATCCGCCCCCTGCACAGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACt      >  2:633406/1‑93 (MQ=255)

                                                                                    tCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACtt     <  1:3734279/93‑1 (MQ=255)

                                                                                     gaGCGTGAACCGCACCACGCTCCGGCCGTCATCAGCGCTGGTTCAGGCGTTGAGTGGGCATTTCGAAGCGCGGTTACCGAAACTGGTCCCTtc    <  2:364176/92‑1 (MQ=255)

                                                                                     cAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTtc    <  1:2008526/93‑1 (MQ=255)

                                                                                       gCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTtctc  >  2:2025570/1‑93 (MQ=255)

CGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTC  >  NC_000913/2977764‑2977943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`