BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,046,045	T→C	V339V (GTA→GTG)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,046,045	0	T	C	98.8%	521.1 / ‑3.3	162	V339V (GTA→GTG)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (76/84); total (77/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 173 total aligned reads displayed.

ATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCC  >  NC_000913/3045955‑3046134

atCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGTCATACAGGCTGGCGATGTTTGCCAGCAGCAc                                                                                         >  2:2376912/1‑93 (MQ=255)

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                        >  1:2077114/1‑93 (MQ=255)

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                        <  2:2040158/93‑1 (MQ=255)

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                        <  1:4398642/93‑1 (MQ=255)

  cGGTCAGACGGTGGATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAGCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCt                                                                                       <  1:84742/93‑1 (MQ=255)

   ggTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTg                                                                                      >  1:2128373/1‑93 (MQ=255)

   ggTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGAACCACCTg                                                                                      >  1:2875712/1‑93 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                     <  1:4228384/93‑1 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                     >  1:3679706/1‑93 (MQ=255)

     tCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTggg                                                                                    <  2:545312/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  1:2093234/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  2:4501002/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  >  2:221820/1‑93 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  1:1183481/93‑1 (MQ=255)

         aCGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGt                                                                                <  1:1103455/93‑1 (MQ=255)

         aCGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGt                                                                                >  1:4422032/1‑93 (MQ=255)

          cGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTa                                                                               >  1:47587/1‑93 (MQ=255)

          cGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTa                                                                               <  1:2431592/93‑1 (MQ=255)

            gTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACTGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACa                                                                             >  1:842523/1‑93 (MQ=255)

              gAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGGTAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACaaa                                                                           <  2:4235863/93‑1 (MQ=255)

                aTGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATg                                                                         >  1:2199421/1‑93 (MQ=255)

                aTGCGGTTAGCGATACGTTTCAGGCCAACCAGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCCGAAGCAACTGGGAAGTACAAATg                                                                         >  2:1239459/1‑93 (MQ=255)

                 tGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGt                                                                        <  1:419539/93‑1 (MQ=255)

                 tGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGt                                                                        >  1:1586428/1‑93 (MQ=255)

                 agtggTTAGCGAGACGTGTCAGGCCAACCGTGCCGTGATAAACGGCATACAGGCTGGCGATGGTTGCCAGCAGCACCTGGGAAGTACAAATGt                                                                        <  1:1008890/90‑1 (MQ=255)

                  gCGGTTAGCGATACGTTTCAGAACAACCGGGACGTGATACACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAAAGtt                                                                       <  1:2694234/93‑1 (MQ=255)

                    ggTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTgg                                                                     <  2:2615816/93‑1 (MQ=255)

                       tAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGt                                                                  <  2:820067/93‑1 (MQ=255)

                        aGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGtt                                                                 <  1:2393421/93‑1 (MQ=255)

                         gCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTc                                                                <  2:3207015/93‑1 (MQ=255)

                         gCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTc                                                                <  1:4494364/93‑1 (MQ=255)

                          cGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCg                                                               <  1:3758018/93‑1 (MQ=255)

                           gATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGc                                                              <  2:925316/93‑1 (MQ=255)

                            aTACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                             >  1:2314337/1‑93 (MQ=255)

                             tACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCtt                                                            <  2:4029613/93‑1 (MQ=255)

                             tACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCtt                                                            <  1:17376/93‑1 (MQ=255)

                              aCGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTAGCCAGCAGGACCTGGGAAGTACTAATGTTGGAGTTCGCttt                                                           >  2:4290647/1‑93 (MQ=255)

                               cGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                          <  1:1056258/93‑1 (MQ=255)

                                gTTTCAGGCCAACCGGGCCGTGATAAACTGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtct                                                         <  2:4108821/93‑1 (MQ=255)

                                gTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtct                                                         <  2:1609619/93‑1 (MQ=255)

                                gTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtct                                                         <  1:1512079/93‑1 (MQ=255)

                                 tttCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtctc                                                        <  1:2667181/93‑1 (MQ=255)

                                   tCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAc                                                      <  2:1736661/93‑1 (MQ=255)

                                    cAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcg                                                     <  2:3740652/93‑1 (MQ=255)

                                     aGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcgg                                                    <  2:859022/93‑1 (MQ=255)

                                      ggCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggc                                                   >  2:1871983/1‑93 (MQ=255)

                                      ggCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggc                                                   >  2:4376874/1‑93 (MQ=255)

                                         cAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGa                                                <  2:4642097/93‑1 (MQ=255)

                                          aaCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGat                                               >  2:3448216/1‑93 (MQ=255)

                                             cGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATg                                            >  2:1131925/1‑93 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                           >  2:3744447/1‑93 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                           >  1:461173/1‑93 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                           >  1:1473208/1‑93 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                           <  1:4064908/93‑1 (MQ=255)

                                               ggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGtt                                          <  2:3431203/93‑1 (MQ=255)

                                                gCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTg                                         >  2:1528759/1‑93 (MQ=255)

                                                 ccGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGc                                        <  1:2504184/93‑1 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                      >  1:1213101/1‑93 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                      >  1:254829/1‑93 (MQ=255)

                                                   gTGATAAACGGCATACAGGATGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                      >  1:3413695/1‑93 (MQ=255)

                                                    tGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGGTATGTTGCTcg                                     >  2:2132665/1‑93 (MQ=255)

                                                     gATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcgc                                    >  1:2763842/1‑93 (MQ=255)

                                                     gATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcgc                                    <  2:3293694/93‑1 (MQ=255)

                                                     gATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcgc                                    <  1:4254605/93‑1 (MQ=255)

                                                     gATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcgc                                    >  2:1067516/1‑93 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  <  1:4157747/93‑1 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  <  1:500559/93‑1 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  >  1:1251036/1‑93 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  >  2:2797439/1‑93 (MQ=255)

                                                         aaCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAgc                                >  2:1068551/1‑92 (MQ=255)

                                                          aaggCATACAGGCTGGCTGTGTTTGCCAGCGGGAGCTAGGAAGTACAAATGTTTGAGTTCGCTTTCTCCCGGCGGATATGTTGCTCGCGAGTc                               <  2:3609434/91‑1 (MQ=255)

                                                          aCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCATGGCGGATATGTTGCTCGCGAGTc                               <  1:3840946/93‑1 (MQ=255)

                                                           cGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCt                              <  1:3018939/93‑1 (MQ=255)

                                                             gCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGc                            >  2:895893/1‑93 (MQ=255)

                                                              cATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCa                           <  1:220474/93‑1 (MQ=255)

                                                               aTACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                          >  1:4647988/1‑93 (MQ=255)

                                                                 aCAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCg                        >  1:3458963/1‑93 (MQ=255)

                                                                 aCAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCg                        <  1:610559/93‑1 (MQ=255)

                                                                 aCAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCg                        >  2:362414/1‑93 (MQ=255)

                                                                  cAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGc                       >  1:551158/1‑93 (MQ=255)

                                                                  cAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGc                       <  1:2963886/93‑1 (MQ=255)

                                                                    ggCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCa                     >  2:4352437/1‑93 (MQ=255)

                                                                    ggCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCa                     >  1:3037523/1‑93 (MQ=255)

                                                                      cTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATg                   <  1:560783/93‑1 (MQ=255)

                                                                      cTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATg                   <  2:4551844/93‑1 (MQ=255)

                                                                           gATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAg              <  2:2874912/93‑1 (MQ=255)

                                                                           gATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAg              >  1:2424372/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             >  1:913070/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             >  1:1340981/1‑93 (MQ=255)

                                                                             tGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcg            >  1:750024/1‑93 (MQ=255)

                                                                             tGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcg            >  2:2754791/1‑93 (MQ=255)

                                                                             tGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcg            <  2:2922375/93‑1 (MQ=255)

                                                                              gTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcgc           >  1:3834931/1‑93 (MQ=255)

                                                                               tttGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGcgcg          >  2:4727218/1‑93 (MQ=255)

                                                                                ttGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTAACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCgg         <  1:450934/93‑1 (MQ=255)

                                                                                  gCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGCCTGCATCGCCATGCGCAGCGCGGTa       >  2:4367310/1‑93 (MQ=255)

                                                                                    cagcagCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAtt     <  2:3090455/93‑1 (MQ=255)

                                                                                      gcagcaCCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGc   <  2:1887587/93‑1 (MQ=255)

                                                                                      gcagcaCCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGc   <  2:3503720/93‑1 (MQ=255)

                                                                                       cagCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCCGTATTGcc  >  2:56086/1‑93 (MQ=255)

ATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCC  >  NC_000913/3045955‑3046134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`