BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,245	C→G	G470G (GGG→GGC)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,245	0	C	G	98.5%	426.2 / NA	140	G470G (GGG→GGC)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base C (0/2); new base G (62/73); total (62/78)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

GCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078156‑3078334

gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCg                                                                                        >  2:2518575/1‑93 (MQ=255)

gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTG‑TCAACCCGGCTGGGGAGCCGGCCCg                                                                                        >  2:3757860/1‑93 (MQ=255)

  ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTc                                                                                      >  2:264884/1‑93 (MQ=255)

       cAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGc                                                                                 <  2:243701/93‑1 (MQ=255)

       cAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGc                                                                                 >  1:1849149/1‑93 (MQ=255)

        caCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                                                <  1:889088/92‑1 (MQ=255)

        caCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                                                <  1:2286878/92‑1 (MQ=255)

        aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                                                <  1:889207/93‑1 (MQ=255)

         aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCcccc                                                                               >  2:1968508/1‑93 (MQ=255)

         aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCACAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGACCGTCTTCGccc                                                                               <  2:3822575/93‑1 (MQ=255)

          ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                                              <  1:409463/93‑1 (MQ=255)

          ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                                              <  2:3320703/93‑1 (MQ=255)

            tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            <  1:1158246/93‑1 (MQ=255)

            tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            >  2:3061956/1‑93 (MQ=255)

             ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                                           >  1:4203333/1‑93 (MQ=255)

              gTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAcc                                                                          <  1:4390778/93‑1 (MQ=255)

                cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGTTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        <  2:3019452/93‑1 (MQ=255)

                cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        <  2:4715119/93‑1 (MQ=255)

                 acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                       >  2:3327148/1‑93 (MQ=255)

                 acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                       >  2:4023044/1‑93 (MQ=255)

                 acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                       <  1:2737995/93‑1 (MQ=255)

                  cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                      <  1:1461284/93‑1 (MQ=255)

                    cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAA‑CGGGCTGGATAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                                                    <  2:3693912/93‑1 (MQ=255)

                    cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAT‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                                                    <  1:2391761/93‑1 (MQ=255)

                    cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                                                    <  2:216593/93‑1 (MQ=255)

                     ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGCCCGGTggcg                                                                   >  2:855803/1‑91 (MQ=255)

                     ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAg                                                                   <  1:2198170/93‑1 (MQ=255)

                      gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                                                  <  2:4614635/93‑1 (MQ=255)

                        cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCg                                                                >  2:4692570/1‑93 (MQ=255)

                         tccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGGCGGGCCGTCTTCGCCCAGACCGGTGGAGTCGt                                                               <  2:2386414/92‑1 (MQ=255)

                         gCCATGTAGTCATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGt                                                               <  2:955880/93‑1 (MQ=255)

                         gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCgg                                                               >  2:4119334/1‑92 (MQ=255)

                         gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                               >  2:3443269/1‑93 (MQ=255)

                         gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                               <  1:186879/93‑1 (MQ=255)

                         gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                               <  1:172843/93‑1 (MQ=255)

                          ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                                              >  2:1982127/1‑93 (MQ=255)

                          ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                                              >  1:3114835/1‑93 (MQ=255)

                          ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                                              >  1:2557411/1‑93 (MQ=255)

                           cATGTAGACATGTTCGGGGTAACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgg                                                             <  2:1835746/93‑1 (MQ=255)

                            aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            <  1:1083608/93‑1 (MQ=255)

                             tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgggg                                                           >  2:1641713/1‑92 (MQ=255)

                             tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                           >  1:3323173/1‑93 (MQ=255)

                             tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                           <  1:4379677/93‑1 (MQ=255)

                               tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                         >  2:956167/1‑93 (MQ=255)

                               tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                         <  1:3618267/93‑1 (MQ=255)

                               gaGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                         <  1:3618266/92‑1 (MQ=255)

                                aGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGGCGTCTTCGCCCAGACCGATGGAGCCGGGGGTGTa                                                        >  2:1000115/1‑93 (MQ=255)

                                   cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                                                     <  1:702381/93‑1 (MQ=255)

                                   cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                                                     >  2:410157/1‑93 (MQ=255)

                                   cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                                                     <  2:1494991/93‑1 (MQ=255)

                                       ttCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATc                                                 >  1:908298/1‑93 (MQ=255)

                                        tCGTGGTTACGGGCAGAGAAGCGACCTGCTCCA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCa                                                <  1:1144762/93‑1 (MQ=255)

                                         cGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAc                                               <  1:3416246/93‑1 (MQ=255)

                                           gggTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                             <  1:4554234/93‑1 (MQ=255)

                                           gggTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                             <  2:3231510/93‑1 (MQ=255)

                                           gggTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                             <  2:3231387/93‑1 (MQ=255)

                                              ttACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                          <  2:688489/93‑1 (MQ=255)

                                              ttACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                          <  1:736930/93‑1 (MQ=255)

                                              ttACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTAAACCATTACCTGAc                                          >  1:2929202/1‑93 (MQ=255)

                                              gtACGCGCAGAGCAGCGCCCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                          <  2:1034123/92‑1 (MQ=255)

                                               tACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAcc                                         >  2:1225367/1‑92 (MQ=255)

                                                 cgcgCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAGGCt                                       >  2:1296693/1‑93 (MQ=255)

                                                   cgcAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGt                                     <  2:4345745/93‑1 (MQ=255)

                                                   cgcAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGt                                     <  2:3547800/93‑1 (MQ=255)

                                                   cgcAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGt                                     <  1:4084975/93‑1 (MQ=255)

                                                    gcAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGtt                                    <  1:1334086/93‑1 (MQ=255)

                                                    gcAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAACTGACGCTGtt                                    >  1:3862276/1‑93 (MQ=255)

                                                     cAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                                   <  1:3376525/93‑1 (MQ=255)

                                                      agagAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc                                  <  1:384097/93‑1 (MQ=255)

                                                      agagAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc                                  <  1:2405559/93‑1 (MQ=255)

                                                      agagAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc                                  >  2:4596657/1‑93 (MQ=255)

                                                       gagaAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                 >  1:3334919/1‑93 (MQ=255)

                                                         gaAGCGACCTGCTCAT‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcata                               <  2:4217138/93‑2 (MQ=255)

                                                           aGCGGCCTGCTCAT‑CCGGCTGGTGAGCCGGTCCGTCTTCGCCCAGACCGATGGAGTCGGGAGTGTAATCCATCTCCTGACGCTGTTTCATCAg                             <  2:4503318/93‑1 (MQ=255)

                                                           aGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGTCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAg                             <  1:1796323/93‑1 (MQ=255)

                                                            gCGACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                            <  2:3845024/93‑1 (MQ=255)

                                                              gACCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc                          >  2:3973104/1‑93 (MQ=255)

                                                               aCCTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCCCCTGACGCTGTTTCATCAGCGCa                         <  1:3489072/93‑1 (MQ=255)

                                                                 cTGCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc                       <  1:4285927/93‑1 (MQ=255)

                                                                   gCTCAC‑CCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACTGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCa                     <  2:23895/93‑1 (MQ=255)

                                                                   gCTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGGGGGTGTAAACCATCACCTGACGCTGGTTGAGCAGCGCAGGCa                     >  2:385243/1‑93 (MQ=255)

                                                                    cTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAt                    >  2:3538027/1‑93 (MQ=255)

                                                                    cTCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAt                    <  2:766211/93‑1 (MQ=255)

                                                                     tCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAta                   >  1:1336576/1‑93 (MQ=255)

                                                                     tCAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAta                   <  1:4672493/93‑1 (MQ=255)

                                                                      cAA‑CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                  <  2:1512818/93‑1 (MQ=255)

                                                                      cAA‑CCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                  <  1:1473095/93‑1 (MQ=255)

                                                                          ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgta               >  2:2738332/1‑93 (MQ=255)

                                                                             gCTTGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCGGTTTCATCAGCGCAGCCATACGTACgg            <  1:1511580/93‑1 (MQ=255)

                                                                             gCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACgg            <  1:2760099/93‑1 (MQ=255)

                                                                             gCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACgg            >  2:2196071/1‑93 (MQ=255)

                                                                               tGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCg          >  2:692339/1‑93 (MQ=255)

                                                                               tGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCg          >  1:3489571/1‑93 (MQ=255)

                                                                               tGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGGCGTGGGTGTACACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCg          >  1:52386/1‑93 (MQ=255)

                                                                                ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGt         >  2:3461800/1‑93 (MQ=255)

                                                                                  tGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTa       >  1:458758/1‑93 (MQ=255)

                                                                                       cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc  >  1:270541/1‑93 (MQ=255)

                                                                                       cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc  <  1:1507063/93‑1 (MQ=255)

                                                                                       cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc  <  1:921293/93‑1 (MQ=255)

                                                                                       cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc  <  1:1015599/93‑1 (MQ=255)

GCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAA‑CCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078156‑3078334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`