BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,160,251	A→G	T145T (ACT→ACC)	sufI ←	suppressor of ftsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,160,251	0	A	G	98.5%	408.8 / ‑3.8	130	T145T (ACT→ACC)	sufI	suppressor of ftsI
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (61/67); total (61/69)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 136 total aligned reads displayed.

GTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGG  >  NC_000913/3160161‑3160340

gTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGt                                                                                         <  2:1653795/93‑1 (MQ=255)

gTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGt                                                                                         <  2:1059960/93‑1 (MQ=255)

gTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGt                                                                                         >  1:3962875/1‑93 (MQ=255)

  tGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTAt                                                                                       <  1:3811270/93‑1 (MQ=255)

   ggggATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTAtt                                                                                      <  1:312385/93‑1 (MQ=255)

       aTAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCg                                                                                  >  1:2994149/1‑93 (MQ=255)

        tAGGCAGCGACTTGCTGACTCCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGt                                                                                 <  2:483826/93‑1 (MQ=255)

          ggCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGa                                                                               >  1:4064843/1‑93 (MQ=255)

          ggCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGa                                                                               >  2:576262/1‑93 (MQ=255)

           gCAGCGCCTTGCTGATTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGAt                                                                              <  2:2663413/93‑1 (MQ=255)

           gCAGCGACTTGCTGACTTCATCTTCCGCCAGCCACATTCCGGCAAGGCCGTTATAGACATGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGAt                                                                              <  2:3356775/93‑1 (MQ=255)

           gCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGAt                                                                              >  2:2669585/1‑93 (MQ=255)

           gCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGAt                                                                              >  1:2250136/1‑93 (MQ=255)

             aGCGACTTGCTGACTTCATCTTCCACCAGCCACATTTCGGCAAGGCCGTTATAGACCTGCTGAACCGTGCGGTTGGGGGTATTGGCGTGATAc                                                                            >  1:2723303/1‑93 (MQ=255)

             aGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATAc                                                                            >  1:3994447/1‑93 (MQ=255)

              gccccTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATAcc                                                                           <  2:3370017/89‑1 (MQ=255)

                gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcac                                                                         >  1:233860/1‑93 (MQ=255)

                gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcac                                                                         >  1:839427/1‑93 (MQ=255)

                 aCTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcaca                                                                        >  1:1418098/1‑93 (MQ=255)

                  cTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACag                                                                       >  1:2424358/1‑93 (MQ=255)

                  cTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACag                                                                       <  2:4316572/93‑1 (MQ=255)

                   ttGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGTGGTCTTGGCGTGATACCACaga                                                                      >  2:39629/1‑93 (MQ=255)

                   ttGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACaga                                                                      >  1:2508943/1‑93 (MQ=255)

                   ttGCTGACTTCATCTCCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACaga                                                                      <  2:4650937/93‑1 (MQ=255)

                    tGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACagag                                                                     <  2:256019/93‑1 (MQ=255)

                    tGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACagag                                                                     <  1:65394/93‑1 (MQ=255)

                     gCTGCCTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGt                                                                    <  2:1250842/93‑1 (MQ=255)

                     gCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGt                                                                    <  2:4455057/93‑1 (MQ=255)

                      cTGCCTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTa                                                                   <  1:4633870/93‑1 (MQ=255)

                      cTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTa                                                                   >  1:1373549/1‑93 (MQ=255)

                      cTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTa                                                                   <  1:4106813/93‑1 (MQ=255)

                       tGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAg                                                                  <  2:198508/93‑1 (MQ=255)

                       tGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAg                                                                  >  2:2025335/1‑93 (MQ=255)

                        gACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGc                                                                 <  2:1823756/93‑1 (MQ=255)

                        gACTTCATCTCCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGc                                                                 <  2:3128608/93‑1 (MQ=255)

                         aCTTCTTCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCt                                                                <  1:3864020/93‑1 (MQ=255)

                         aCTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCt                                                                <  1:1324846/93‑1 (MQ=255)

                         aCTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCt                                                                >  1:4275719/1‑93 (MQ=255)

                          cTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTg                                                               >  1:3747735/1‑93 (MQ=255)

                            tCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCg                                                             <  2:4454765/93‑1 (MQ=255)

                              aTCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGtt                                                           <  1:514411/93‑1 (MQ=255)

                               tCTTCCACCAGCCACATTCCGGCGAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTc                                                          <  2:2595710/93‑1 (MQ=255)

                                cTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCt                                                         <  1:2587592/93‑1 (MQ=255)

                                 ttCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTg                                                        >  1:1928831/1‑93 (MQ=255)

                                   ccaccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTggg                                                      >  1:1754836/1‑92 (MQ=255)

                                     accaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGa                                                    >  1:2812568/1‑93 (MQ=255)

                                      ccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGaa                                                   <  1:4075113/93‑1 (MQ=255)

                                       caGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAt                                                  >  1:4176704/1‑93 (MQ=255)

                                       caGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAt                                                  <  1:3769292/93‑1 (MQ=255)

                                       caGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAt                                                  <  1:3422619/93‑1 (MQ=255)

                                         gCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATgg                                                >  1:4506092/1‑93 (MQ=255)

                                           cacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGc                                              >  2:4140555/1‑93 (MQ=255)

                                           cacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGc                                              <  1:3194613/93‑1 (MQ=255)

                                           cacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACACAGGAGCTGCGTTCTGGCGACTGGGc                                              >  2:2422019/1‑93 (MQ=255)

                                            acaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCa                                             <  1:4395410/93‑1 (MQ=255)

                                            acaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCa                                             <  2:1293826/93‑1 (MQ=255)

                                            acaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCa                                             <  1:143880/93‑1 (MQ=255)

                                            acaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCa                                             >  2:2464113/1‑93 (MQ=255)

                                            acaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGAATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCa                                             >  2:1495601/1‑93 (MQ=255)

                                             caTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAg                                            <  1:1956511/93‑1 (MQ=255)

                                             caTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAg                                            <  2:1320213/93‑1 (MQ=255)

                                              aTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGt                                           >  1:2718738/1‑93 (MQ=255)

                                                   ggCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                      <  1:4022321/93‑1 (MQ=255)

                                                   ggCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGGATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                      >  2:1963988/1‑93 (MQ=255)

                                                   ggCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                      <  2:2706204/93‑1 (MQ=255)

                                                     cAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTg                                    >  2:3189503/1‑93 (MQ=255)

                                                      aaGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGc                                   >  1:2520271/1‑93 (MQ=255)

                                                      aaGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGc                                   >  1:1721051/1‑93 (MQ=255)

                                                       aGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGcc                                  >  1:3908877/1‑93 (MQ=255)

                                                         gCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCa                                <  1:1258325/93‑1 (MQ=255)

                                                         gCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGGGCCCa                                >  2:2102955/1‑93 (MQ=255)

                                                          ccGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAg                               >  2:1889946/1‑93 (MQ=255)

                                                            gTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                             <  1:1860787/93‑1 (MQ=255)

                                                            gTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                             <  1:613508/93‑1 (MQ=255)

                                                            gTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                             <  2:4289868/93‑1 (MQ=255)

                                                            gTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                             >  2:3344110/1‑93 (MQ=255)

                                                            gTTATAGACCTGCTGAACCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGAAGCTGCGTTCTGGCGTATGGGCAGTACGGGTCCCCAGtt                             >  2:3389260/1‑92 (MQ=255)

                                                             ttATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                            >  2:1276/1‑93 (MQ=255)

                                                                taGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCg                         <  2:124779/93‑1 (MQ=255)

                                                                 aGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGt                        >  2:197456/1‑93 (MQ=255)

                                                                 aGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGt                        <  2:774389/93‑1 (MQ=255)

                                                                  gACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGtt                       <  2:3477901/93‑1 (MQ=255)

                                                                  gACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGtt                       >  2:3851897/1‑93 (MQ=255)

                                                                    ccTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTg                     <  2:1724055/93‑1 (MQ=255)

                                                                     ctgctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTgg                    >  2:1659731/1‑93 (MQ=255)

                                                                     ctgctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTgg                    <  2:3856666/93‑1 (MQ=255)

                                                                     ctgctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTgg                    >  2:2765792/1‑93 (MQ=255)

                                                                      tgctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGc                   >  2:4350790/1‑93 (MQ=255)

                                                                       gctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCg                  >  1:624256/1‑93 (MQ=255)

                                                                         tgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAc                >  2:1126753/1‑93 (MQ=255)

                                                                         tgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAc                <  1:413197/93‑1 (MQ=255)

                                                                            gCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatc             <  2:4245880/93‑1 (MQ=255)

                                                                              cGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatcat           <  2:2331765/93‑1 (MQ=255)

                                                                               gTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc          <  2:2666759/93‑1 (MQ=255)

                                                                                tGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATcc         >  1:1299425/1‑93 (MQ=255)

                                                                                 gCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCg        <  2:2844661/93‑1 (MQ=255)

                                                                                  cGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGt       <  2:2251120/93‑1 (MQ=255)

                                                                                   ggTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTg      >  2:3397613/1‑93 (MQ=255)

                                                                                       ggggGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCgg  <  2:565487/93‑1 (MQ=255)

                                                                                       ggggGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCgg  <  2:781717/93‑1 (MQ=255)

GTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGG  >  NC_000913/3160161‑3160340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`