BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,185,776	T→C	F121L (TTC→CTC)	yqiG →	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,185,776	0	T	C	100.0%	1051.9 / NA	316	F121L (TTC→CTC)	yqiG	putative membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (169/147); total (169/147)

Only 100 of 325 total aligned reads displayed.

GGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGG  >  NC_000913/3185686‑3185865

 gATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCg                                                                                        >  2:54721/1‑93 (MQ=255)

 gATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCg                                                                                        <  2:421821/93‑1 (MQ=255)

 gATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCg                                                                                        >  1:4345638/1‑93 (MQ=255)

 gATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCg                                                                                        >  1:952131/1‑93 (MQ=255)

   tttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGAt                                                                                      <  1:3239533/93‑1 (MQ=255)

   tttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGAt                                                                                      <  2:3139564/93‑1 (MQ=255)

     tACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATaa                                                                                    <  1:3645226/93‑1 (MQ=255)

       cTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAg                                                                                  <  1:4290086/93‑1 (MQ=255)

        tgatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAgg                                                                                 >  1:2350762/1‑93 (MQ=255)

         gatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  2:87960/1‑93 (MQ=255)

         gatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  1:1780293/1‑93 (MQ=255)

         gatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                <  2:3356180/93‑1 (MQ=255)

          atgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGa                                                                               >  1:1873598/1‑93 (MQ=255)

          atgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGa                                                                               >  2:2499780/1‑93 (MQ=255)

          atgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGa                                                                               <  1:895777/93‑1 (MQ=255)

          atgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGa                                                                               <  1:3626593/93‑1 (MQ=255)

           tgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaa                                                                              >  2:1076978/1‑93 (MQ=255)

             aaTTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaaaa                                                                            <  2:1974256/93‑1 (MQ=255)

                ttATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGc                                                                         >  2:3687856/1‑93 (MQ=255)

                  aTTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCaa                                                                       <  1:2601485/93‑1 (MQ=255)

                  aTTGAAAAAACTCAGCAAAACCTGATCGATGGGTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCaa                                                                       >  2:2130069/1‑93 (MQ=255)

                    tGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAAtt                                                                     >  1:2405480/1‑93 (MQ=255)

                     gAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTa                                                                    >  2:3849522/1‑93 (MQ=255)

                      aaaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTAt                                                                   <  2:4495918/93‑1 (MQ=255)

                       aaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATc                                                                  >  1:2739173/1‑93 (MQ=255)

                        aaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATcc                                                                 >  1:1068207/1‑93 (MQ=255)

                         aaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCa                                                                >  1:741156/1‑93 (MQ=255)

                          aaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCat                                                               >  1:225852/1‑93 (MQ=255)

                           aCTCAGCAAACCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCata                                                              <  1:2672861/93‑1 (MQ=255)

                            cTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCatat                                                             <  1:3045557/93‑1 (MQ=255)

                            cTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCatat                                                             >  1:557473/1‑93 (MQ=255)

                             tCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCatatc                                                            <  2:793551/93‑2 (MQ=255)

                             tCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCatatc                                                            >  2:1924363/1‑92 (MQ=255)

                              cAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCatatct                                                           >  2:2640092/1‑91 (MQ=255)

                                gCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGc                                                         <  1:3072298/93‑1 (MQ=255)

                                 cAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGTAAATGCAATTATCCATATCTGCa                                                        >  2:2583562/1‑93 (MQ=255)

                                  aaaaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCAc                                                       >  1:1360440/1‑93 (MQ=255)

                                   aaaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCAcc                                                      <  2:2894754/93‑1 (MQ=255)

                                    aaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCt                                                     >  1:1122917/1‑93 (MQ=255)

                                    aaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCt                                                     <  2:2059840/93‑1 (MQ=255)

                                    aaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCt                                                     <  2:407021/93‑1 (MQ=255)

                                     aCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTc                                                    >  2:2656747/1‑93 (MQ=255)

                                      ccTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCa                                                   <  2:2982469/93‑1 (MQ=255)

                                      ccTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCa                                                   <  1:2552729/93‑1 (MQ=255)

                                      ccTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCa                                                   <  1:3577390/93‑1 (MQ=255)

                                       cTGATCGATTGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAg                                                  <  2:1219940/93‑1 (MQ=255)

                                       cTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAg                                                  >  2:2323035/1‑93 (MQ=255)

                                        tGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAgg                                                 <  1:477060/93‑1 (MQ=255)

                                        tGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAgg                                                 >  2:318398/1‑93 (MQ=255)

                                         gATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGc                                                >  2:1857481/1‑93 (MQ=255)

                                          aTCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCa                                               <  2:1658208/93‑1 (MQ=255)

                                          aTCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCa                                               <  2:1583628/93‑1 (MQ=255)

                                          aTCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCa                                               <  2:904960/93‑1 (MQ=255)

                                            cGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATg                                             <  1:291763/93‑1 (MQ=255)

                                               tGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGtt                                          >  1:2007063/1‑93 (MQ=255)

                                               tGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGtt                                          >  2:4416682/1‑93 (MQ=255)

                                                 gttgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaa                                        <  2:657572/93‑1 (MQ=255)

                                                    gttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAt                                     >  1:3869504/1‑93 (MQ=255)

                                                    gttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAt                                     <  2:1541187/93‑1 (MQ=255)

                                                       aCCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACa                                  >  1:1575861/1‑93 (MQ=255)

                                                       aCCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACa                                  >  1:3169355/1‑93 (MQ=255)

                                                        cccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaa                                 <  1:3293059/93‑1 (MQ=255)

                                                        cccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaa                                 >  1:2051459/1‑93 (MQ=255)

                                                         ccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaaa                                >  1:1471709/1‑93 (MQ=255)

                                                         ccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaaa                                >  2:2766714/1‑93 (MQ=255)

                                                         ccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaaa                                <  2:3289897/93‑1 (MQ=255)

                                                          cTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAg                               >  1:4497236/1‑93 (MQ=255)

                                                          cTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAg                               <  1:1685868/93‑1 (MQ=255)

                                                          cTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAg                               >  1:1292001/1‑93 (MQ=255)

                                                           tATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGa                              >  2:698647/1‑93 (MQ=255)

                                                           tATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGa                              <  1:3739042/93‑1 (MQ=255)

                                                             tCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATg                            >  1:4677928/1‑93 (MQ=255)

                                                             tCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATg                            <  1:7061/93‑1 (MQ=255)

                                                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                          >  1:1152483/1‑93 (MQ=255)

                                                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                          >  1:295919/1‑93 (MQ=255)

                                                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                          >  1:2110941/1‑93 (MQ=255)

                                                                 aaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCaaa                        <  1:4433606/93‑1 (MQ=255)

                                                                  aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                       >  2:1378388/1‑93 (MQ=255)

                                                                   aagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACt                      >  1:4219587/1‑93 (MQ=255)

                                                                    agaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTg                     >  1:2157999/1‑93 (MQ=255)

                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  <  2:1685373/93‑1 (MQ=255)

                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  1:599461/1‑93 (MQ=255)

                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  2:3943546/1‑93 (MQ=255)

                                                                          aCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcc               <  2:4654907/93‑1 (MQ=255)

                                                                          aCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcc               <  1:31098/93‑1 (MQ=255)

                                                                           cAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcct              <  2:49724/93‑1 (MQ=255)

                                                                            aaaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctc             <  2:2828157/93‑1 (MQ=255)

                                                                            aaaTTACAACTTATCTCGATAAAGGGAAAATACAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctc             >  2:3869718/1‑93 (MQ=255)

                                                                             aaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcc            >  2:3997507/1‑93 (MQ=255)

                                                                             aaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcc            >  2:3559573/1‑93 (MQ=255)

                                                                             aaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcc            >  1:1006411/1‑93 (MQ=255)

                                                                              aTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcct           <  1:3381767/93‑1 (MQ=255)

                                                                              aTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcct           <  2:3028676/93‑1 (MQ=255)

                                                                              aTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcct           >  2:2431489/1‑93 (MQ=255)

                                                                                tACAACTTATCTCAATGAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGa         <  1:3976618/93‑1 (MQ=255)

                                                                                 aCAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGaa        >  2:3220896/1‑93 (MQ=255)

                                                                                 aCAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGaa        >  1:2781492/1‑93 (MQ=255)

                                                                                    aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt     >  2:3649340/1‑93 (MQ=255)

                                                                                    aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt     >  2:1316472/1‑93 (MQ=255)

                                                                                     cTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACttt    >  1:3914781/1‑93 (MQ=255)

GGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGG  >  NC_000913/3185686‑3185865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`