BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,212,918	A→G	V66A (GTC→GCC)	ygjF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,212,918	0	A	G	98.1%	483.4 / ‑1.7	158	V66A (GTC→GCC)	ygjF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (78/77); total (80/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 163 total aligned reads displayed.

ATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCA  >  NC_000913/3212828‑3213003

aTTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCg                                                                                     >  2:4348639/1‑93 (MQ=255)

 ttttttCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCCGTTTTGTGgcgc                                                                                    >  2:3483739/1‑93 (MQ=255)

    tttCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCcac                                                                                 >  1:2271964/1‑93 (MQ=255)

      tCAATCAGCTACCGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTCCCAGTTTGGTGGCGCCACaa                                                                               <  1:2471695/93‑1 (MQ=255)

       cAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACaat                                                                              <  2:2879345/93‑2 (MQ=255)

       cAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAAc                                                                              <  2:1757207/93‑1 (MQ=255)

       cAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAAc                                                                              <  1:177000/93‑1 (MQ=255)

        aaTCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACg                                                                             <  2:799920/93‑1 (MQ=255)

         aTCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGa                                                                            <  2:1994069/93‑1 (MQ=255)

         aTCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGa                                                                            >  1:1334224/1‑93 (MQ=255)

          tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGTTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTAACAGTTTGGTGGCGCCACAACGAt                                                                           >  2:633679/1‑93 (MQ=255)

            accTTACGCCCGCCTGGGTGTAGCCCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACAATaa                                                                         <  2:1943421/91‑1 (MQ=255)

            aGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACCACGATaa                                                                         >  1:2869305/1‑93 (MQ=255)

              cTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATc                                                                       <  1:4305413/93‑1 (MQ=255)

               ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATcc                                                                      >  1:4294633/1‑93 (MQ=255)

               ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATcc                                                                      >  1:3202892/1‑93 (MQ=255)

               ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGGTGGACGGTTCACCAGTTTTGTGGCGCCACAAGGATAATcc                                                                      >  1:3202891/1‑93 (MQ=255)

                 aCGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCACCACAACGATAATCcag                                                                    <  2:3318074/93‑1 (MQ=255)

                  cgcgcgctTGCGTGTATCGCTTGCGGTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTCCGAGTTTGGTGGCGCCACAACGATAATCcagc                                                                   <  2:2444217/93‑1 (MQ=255)

                  cgcccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagc                                                                   >  2:1211298/1‑93 (MQ=255)

                   gcccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagca                                                                  >  1:4324325/1‑93 (MQ=255)

                   gcccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagca                                                                  >  2:2767584/1‑93 (MQ=255)

                    cccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagcag                                                                 >  2:623219/1‑93 (MQ=255)

                    cccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCcagcag                                                                 >  1:1967020/1‑93 (MQ=255)

                    cccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCAACGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagccg                                                                 >  1:1568867/1‑91 (MQ=255)

                     ccgccTGCGTGTAGCTCCTGCTTTGAAAATTCATTGGCTTGCACCGTTGGCAGGTGTGACAGATTGGTGGTGGCCCAACGATAATCCAGCAGa                                                                >  2:3757856/1‑93 (MQ=255)

                      cgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGAt                                                               <  2:4664711/93‑1 (MQ=255)

                      cgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGAt                                                               <  2:240615/93‑1 (MQ=255)

                      cgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGAt                                                               >  1:3380169/1‑93 (MQ=255)

                        ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGc                                                             <  1:3938638/93‑1 (MQ=255)

                        ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGc                                                             <  1:1967003/93‑1 (MQ=255)

                        ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGc                                                             <  1:4384508/93‑1 (MQ=255)

                        ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGc                                                             <  2:3031986/93‑1 (MQ=255)

                        ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGCTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGc                                                             <  2:2491318/93‑1 (MQ=255)

                         cTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCt                                                            >  1:4228117/1‑93 (MQ=255)

                          tGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCtg                                                           >  2:4416358/1‑93 (MQ=255)

                          tGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCtg                                                           >  1:2402086/1‑93 (MQ=255)

                          tGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCtg                                                           <  2:234026/93‑1 (MQ=255)

                          tGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCtg                                                           >  2:4412943/1‑93 (MQ=255)

                             gtgtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGc                                                        >  2:2933270/1‑93 (MQ=255)

                              tgtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcc                                                       <  2:723643/93‑1 (MQ=255)

                              tgtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcc                                                       >  2:3921225/1‑93 (MQ=255)

                                 aGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctcc                                                    >  1:1337977/1‑93 (MQ=255)

                                 aGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctcc                                                    <  1:1522417/93‑1 (MQ=255)

                                 aGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctcc                                                    <  1:619825/93‑1 (MQ=255)

                                  gCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctcct                                                   <  1:3297162/93‑1 (MQ=255)

                                    tCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCCCAACGATAATCCAGCAGATGCTGTGCCTCCTGc                                                 <  1:3045360/93‑1 (MQ=255)

                                    tCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATACTCCCGCAGATGCTGTGCCTCCTGc                                                 >  1:1409084/1‑93 (MQ=255)

                                      cTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCgg                                               >  2:3360551/1‑93 (MQ=255)

                                      cTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCgg                                               >  2:2178591/1‑93 (MQ=255)

                                       tGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCGGATGCTGTGCCTCCTGCGGc                                              >  2:1596662/1‑93 (MQ=255)

                                        gCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCt                                             <  2:4244484/93‑1 (MQ=255)

                                         cTTTGAAACTTCGTTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCtt                                            <  2:741996/93‑1 (MQ=255)

                                         cTTTGAAACTTCATTGGCTTGCACCGTTGGACTGTCTACCAGTTTGGTGGCGCCACAACTATAATCCAGCTGAATCCTTTCCTCCTGCGGCtt                                            >  1:4197565/1‑93 (MQ=255)

                                         cTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCtt                                            <  1:3664038/93‑1 (MQ=255)

                                          tttGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTc                                           >  1:2540149/1‑93 (MQ=255)

                                          tttGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTc                                           <  2:3072625/93‑1 (MQ=255)

                                           ttGAAACTTCATTGGCTTGCAACGTTGGACGGTCTACCTGTTTGGTGGCGCCACAAAGATAATCCAGCAGATGGTGTGTCTTCTGCGGCTTCa                                          >  2:2771904/1‑93 (MQ=255)

                                             gAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAAc                                        <  1:4593236/93‑1 (MQ=255)

                                             gAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAAc                                        <  2:4599793/93‑1 (MQ=255)

                                              aaaCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACt                                       <  1:4534819/93‑1 (MQ=255)

                                              aaaCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACt                                       >  2:1922225/1‑93 (MQ=255)

                                               aaCTTCATTGGCTTGCACCGTTGGACGGTCTACCCGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTg                                      >  1:2134303/1‑93 (MQ=255)

                                               aaCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTg                                      >  1:1077774/1‑93 (MQ=255)

                                               aaCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTg                                      >  1:3002970/1‑93 (MQ=255)

                                                aCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGa                                     >  2:1121176/1‑93 (MQ=255)

                                                aCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGa                                     >  1:4693039/1‑93 (MQ=255)

                                                aCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGa                                     >  1:3315529/1‑93 (MQ=255)

                                                aCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGa                                     <  1:1249841/93‑1 (MQ=255)

                                                aCTTCATTGGCTTGCACCGTTGGACGGTCTACCAATTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCGACTGa                                     >  2:2974006/1‑93 (MQ=255)

                                                 cTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTTAACCGAc                                    >  2:1478736/1‑93 (MQ=255)

                                                 cTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAc                                    <  2:3097009/93‑1 (MQ=255)

                                                  ttCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcg                                   >  2:2284991/1‑93 (MQ=255)

                                                     aTTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtc                                >  2:58221/1‑93 (MQ=255)

                                                      ttGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCACCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTCCAACTGAcggtcg                               >  1:4059438/1‑93 (MQ=255)

                                                       tGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCCCAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtcgg                              <  1:3699088/93‑1 (MQ=255)

                                                       tGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtcgg                              <  1:1134132/93‑1 (MQ=255)

                                                         gCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTa                            <  1:4709599/93‑1 (MQ=255)

                                                           ttGCACCGTTGGACGGTCTACCAGTTTGGTGGTGCCACAACGATAATACAGCAGATGCTGTGCCTCCTGCGGATTCAACTGACGGTCGGGaaa                          >  2:708710/1‑93 (MQ=255)

                                                           ttGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATTCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTaaa                          >  2:1835310/1‑93 (MQ=255)

                                                           ttGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCTGTaaa                          >  1:1737446/1‑93 (MQ=255)

                                                            tGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAAc                         <  1:142493/93‑1 (MQ=255)

                                                              cACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAAccc                       >  1:4079269/1‑93 (MQ=255)

                                                               aCCGTTGTACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCg                      <  1:2357660/93‑1 (MQ=255)

                                                                ccGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCgg                     >  1:2133127/1‑93 (MQ=255)

                                                                 cGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGc                    >  1:2126456/1‑93 (MQ=255)

                                                                   ttGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCt                  <  1:2079091/93‑1 (MQ=255)

                                                                     ggACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGa                <  1:1698052/93‑1 (MQ=255)

                                                                        cGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGatat             <  1:651481/93‑1 (MQ=255)

                                                                         ggTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGatata            <  2:4146352/93‑1 (MQ=255)

                                                                           tCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATc          <  1:935444/93‑1 (MQ=255)

                                                                           tCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATc          <  2:4314102/93‑1 (MQ=255)

                                                                             tACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCAc        >  1:2940820/1‑93 (MQ=255)

                                                                               ccAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCt      >  2:3811098/1‑93 (MQ=255)

                                                                               ccAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCt      <  1:2118669/93‑1 (MQ=255)

                                                                               ccAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCt      <  1:4355661/93‑1 (MQ=255)

                                                                                cAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCACCTGACGCACGGTAACCACGGCCTGATATATCACCtt     >  1:1635899/1‑93 (MQ=255)

                                                                                cAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCtt     >  2:2359407/1‑93 (MQ=255)

                                                                                 aGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTc    >  1:3213212/1‑93 (MQ=255)

                                                                                   tttGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCa  <  2:359765/93‑1 (MQ=255)

ATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCA  >  NC_000913/3212828‑3213003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`