Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,221,280 T→C L336P (CTG→CCG)  b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,221,2800TC96.6% 538.4 / 2.7 176L336P (CTG→CCG) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base C (85/84);  total (88/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

ACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAG  >  NC_000913/3221190‑3221368
                                                                                          |                                                                                        
aCGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGAtgccgc                                                                                        >  1:4271921/1‑93 (MQ=255)
  gTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAc                                                                                      <  2:4561547/93‑1 (MQ=255)
   ttCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACg                                                                                     <  1:4217210/93‑1 (MQ=255)
   ttCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACg                                                                                     <  1:4517545/93‑1 (MQ=255)
     cTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACggg                                                                                   >  1:1390892/1‑92 (MQ=255)
      tGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCg                                                                                  >  1:317184/1‑93 (MQ=255)
        gAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTc                                                                                >  2:4258004/1‑93 (MQ=255)
          aGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCaa                                                                              <  1:3248863/93‑1 (MQ=255)
            tggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAAcc                                                                            >  2:3730680/1‑93 (MQ=255)
                   acaACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGa                                                                     <  2:3417150/93‑1 (MQ=255)
                   acaACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGa                                                                     >  2:2506698/1‑93 (MQ=255)
                    caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGAc                                                                    <  1:1591423/93‑1 (MQ=255)
                    caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGAc                                                                    <  2:1131306/93‑1 (MQ=255)
                    caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGAc                                                                    >  1:309251/1‑93 (MQ=255)
                    caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGAc                                                                    >  2:1126976/1‑93 (MQ=255)
                    caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGAc                                                                    >  1:4094029/1‑93 (MQ=255)
                    caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGAc                                                                    >  2:3835646/1‑93 (MQ=255)
                     aaCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACa                                                                   >  1:2781205/1‑93 (MQ=255)
                      aCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACaa                                                                  >  1:1368040/1‑93 (MQ=255)
                        gcgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACg                                                                <  2:4121088/93‑1 (MQ=255)
                         cgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGa                                                               >  1:1774557/1‑93 (MQ=255)
                          gTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGat                                                              <  1:351976/93‑1 (MQ=255)
                           ttGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatc                                                             >  2:1654729/1‑93 (MQ=255)
                           ttGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatc                                                             >  2:2833394/1‑93 (MQ=255)
                           ttGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatc                                                             >  1:3989049/1‑93 (MQ=255)
                            tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatca                                                            >  1:2261700/1‑93 (MQ=255)
                            tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatca                                                            >  1:2261699/1‑93 (MQ=255)
                            tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTCTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatca                                                            <  1:4565921/93‑1 (MQ=255)
                               cTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCg                                                         >  2:3883734/1‑93 (MQ=255)
                               cTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCg                                                         >  2:3883735/1‑93 (MQ=255)
                               cTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCg                                                         >  2:4726594/1‑93 (MQ=255)
                               cTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCg                                                         >  1:1247031/1‑93 (MQ=255)
                                 tCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCa                                                       >  2:2097802/1‑93 (MQ=255)
                                  ccGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaa                                                      <  2:263685/93‑1 (MQ=255)
                                   cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaaa                                                     >  1:2392517/1‑93 (MQ=255)
                                   cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaaa                                                     >  2:808808/1‑93 (MQ=255)
                                    gTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAg                                                    <  2:4033753/93‑1 (MQ=255)
                                    gTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAg                                                    <  1:1412621/93‑1 (MQ=255)
                                    gTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAg                                                    >  1:4184951/1‑93 (MQ=255)
                                    gTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCATCACGACAACGATCATCGCAAAg                                                    >  2:2596137/1‑93 (MQ=255)
                                    gTGATATCAAAGTGCGCGACGGTATGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAg                                                    <  2:2613650/93‑1 (MQ=255)
                                     tGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAgg                                                   >  2:3536086/1‑93 (MQ=255)
                                      tgtatCACAGAGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGc                                                  <  2:2625753/91‑1 (MQ=255)
                                      gATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGc                                                  <  2:4488841/93‑1 (MQ=255)
                                       atatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGcc                                                 <  1:3892508/93‑1 (MQ=255)
                                       atatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGACAACAGTCACGACAACGATCATCGCGAAGGcc                                                 >  2:1602869/1‑93 (MQ=255)
                                        tatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcg                                                >  2:1362632/1‑93 (MQ=255)
                                         atCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcgc                                               >  1:2127598/1‑93 (MQ=255)
                                          tCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcgcg                                              >  2:614584/1‑93 (MQ=255)
                                          tCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcgcc                                              >  2:1632423/1‑92 (MQ=255)
                                          tCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGACACGACAACGATCATCGCAAAGGCcgcg                                              <  1:1786181/93‑1 (MQ=255)
                                           cAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCgcgc                                             <  2:2027496/93‑1 (MQ=255)
                                            aaaGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGcc                                            >  2:1435432/1‑93 (MQ=255)
                                             aaGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAgg                                                   <  1:504896/85‑1 (MQ=255)
                                             aaGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCg                                           <  2:2300696/93‑1 (MQ=255)
                                              aGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGt                                          >  1:610041/1‑93 (MQ=255)
                                               gTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGtt                                         >  1:613438/1‑93 (MQ=255)
                                               gTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGtt                                         <  2:4389734/93‑1 (MQ=255)
                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                     >  1:4393244/1‑93 (MQ=255)
                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                     <  1:2519067/93‑1 (MQ=255)
                                                    cgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAAt                                    >  1:73755/1‑93 (MQ=255)
                                                    cgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAAt                                    <  1:3639533/93‑1 (MQ=255)
                                                     gACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATg                                   <  1:1871865/93‑1 (MQ=255)
                                                      aCGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATgg                                  <  1:4711483/93‑1 (MQ=255)
                                                      aCGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATgg                                  >  1:443101/1‑93 (MQ=255)
                                                       cGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGa                                 <  1:106438/93‑1 (MQ=255)
                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                <  2:3440007/93‑1 (MQ=255)
                                                           cTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATcgc                             >  2:1044771/1‑93 (MQ=255)
                                                             gTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGt                           >  2:1331703/1‑93 (MQ=255)
                                                              ttCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTc                          <  2:3106085/93‑1 (MQ=255)
                                                               tCTGGCTCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCg                         <  2:3260642/93‑1 (MQ=255)
                                                               tCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCg                         <  1:4520005/93‑1 (MQ=255)
                                                                cTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCga                        >  1:3302187/1‑93 (MQ=255)
                                                                 tGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCgag                       >  1:3864684/1‑93 (MQ=255)
                                                                    aTCAATAATCGTTATTTGATGCAGCACGGAGTCAACTGTCACGACAACGATCATCGCAAAGGCCGCTCCGTTGGAATGGATCGCGTCGAGaaa                    <  1:3940847/93‑1 (MQ=255)
                                                                    aTCAATAACCGTTATTTGATGCCGCACGGCTTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCCTCGAGaaa                    <  2:2267732/93‑1 (MQ=255)
                                                                     tCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAg                   >  2:3691824/1‑93 (MQ=255)
                                                                     tCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAg                   >  1:869444/1‑93 (MQ=255)
                                                                     tCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAg                   >  2:1796255/1‑93 (MQ=255)
                                                                     acattAATCGTTATGTGATGCTGCACGGCGTCAACCGTCTCGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAg                   >  2:4331398/5‑93 (MQ=255)
                                                                       aaTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAt                 >  1:2758461/1‑93 (MQ=255)
                                                                       aaTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAt                 >  1:3411655/1‑93 (MQ=255)
                                                                        aTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtc                >  2:335369/1‑93 (MQ=255)
                                                                        aTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAACAtc                <  1:132050/93‑1 (MQ=255)
                                                                          aaCCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtctc              >  2:909852/1‑93 (MQ=255)
                                                                            ccGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCa            >  2:1695607/1‑93 (MQ=255)
                                                                             cGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAg           >  1:2447331/1‑93 (MQ=255)
                                                                              gTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGt          <  2:49354/93‑1 (MQ=255)
                                                                               ttATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGtt         <  2:3111015/93‑1 (MQ=255)
                                                                                tATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtg        >  1:4383005/1‑93 (MQ=255)
                                                                                tATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtg        >  2:3182801/1‑93 (MQ=255)
                                                                                 aTGTGATGCCGCACGGCGTCACCCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtga       <  1:2039350/93‑1 (MQ=255)
                                                                                  tgtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgat      >  2:2428776/1‑93 (MQ=255)
                                                                                  tgtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgat      >  1:2007874/1‑93 (MQ=255)
                                                                                  tgtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGGCGAGAAAGATCCCCAGTtgat      >  1:3308426/1‑93 (MQ=255)
                                                                                   gtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgatg     <  1:280709/93‑1 (MQ=255)
                                                                                    tgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgatga    <  1:1007480/93‑1 (MQ=255)
                                                                                     gATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGaa   <  2:4548374/93‑1 (MQ=255)
                                                                                      aTGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAag  <  2:533561/93‑1 (MQ=255)
                                                                                      aTGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAag  <  1:1134201/93‑1 (MQ=255)
                                                                                          |                                                                                        
ACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAG  >  NC_000913/3221190‑3221368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑