BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,228,614	G→A	A696T (GCT→ACT)	ygjK →	putative isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,228,614	0	G	A	99.5%	620.7 / NA	182	A696T (GCT→ACT)	ygjK	putative isomerase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (94/87); total (95/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

GCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGGCTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGTGAT  >  NC_000913/3228524‑3228706

gCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACt                                                                                            <  2:3472850/93‑1 (MQ=255)

 ccAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACtt                                                                                           <  2:958794/93‑1 (MQ=255)

   aaTGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACtttt                                                                                         >  2:2175011/1‑93 (MQ=255)

   aaTGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACtttt                                                                                         <  1:3499720/93‑1 (MQ=255)

   aaTGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACtttt                                                                                         >  2:4186483/1‑93 (MQ=255)

    aTGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGGCTTTTg                                                                                        >  1:1245902/1‑93 (MQ=255)

      gCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGgc                                                                                      <  1:3139930/93‑1 (MQ=255)

      gCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGgc                                                                                      >  1:2053569/1‑93 (MQ=255)

        cGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGgcgc                                                                                    >  1:1945753/1‑93 (MQ=255)

         gACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCt                                                                                   >  1:3653460/1‑93 (MQ=255)

         gACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCt                                                                                   <  2:1351586/93‑1 (MQ=255)

            gcgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGat                                                                                <  1:1145543/93‑1 (MQ=255)

             cgGTGGTGAATGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGata                                                                               <  1:994598/93‑1 (MQ=255)

              ggtggtGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGatat                                                                              >  1:2620627/1‑93 (MQ=255)

              ggtggtGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGatat                                                                              <  2:2695161/93‑1 (MQ=255)

               gtggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATc                                                                             >  2:2659979/1‑93 (MQ=255)

                 ggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCGGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTa                                                                           <  2:4307576/93‑1 (MQ=255)

                  gtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTAc                                                                          <  1:3419398/93‑1 (MQ=255)

                   tgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACt                                                                         <  2:2874953/93‑1 (MQ=255)

                     aaGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTCGCGCTGATATCTACTgg                                                                       >  2:3847933/1‑93 (MQ=255)

                       ggTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCg                                                                     >  2:2813299/1‑93 (MQ=255)

                       ggTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCg                                                                     <  1:4150448/93‑1 (MQ=255)

                         tGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTg                                                                   <  1:4447804/93‑1 (MQ=255)

                          taTGTTCGATACTAAAGAGTTCAGCTCTTTTGTCCCGCGGGGATCGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTgg                                                                  <  1:3325138/92‑1 (MQ=255)

                             gCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTgggag                                                               >  1:2993693/1‑91 (MQ=255)

                               tCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGcgcg                                                             >  2:1653138/1‑93 (MQ=255)

                               tCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGcgcg                                                             >  1:2289708/1‑93 (MQ=255)

                                ctaTCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGt                                                            >  1:4720507/3‑93 (MQ=255)

                                 gATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                           >  1:1649343/1‑93 (MQ=255)

                                 gATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                           <  2:2050701/93‑1 (MQ=255)

                                 gATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                           <  1:4387723/93‑1 (MQ=255)

                                 gATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                           <  1:4302457/93‑1 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                         >  1:2892769/1‑93 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                         <  1:1703845/93‑1 (MQ=255)

                                    ccTAAAGAGTTCACCACCTTTGTCCCGCTGGGACCGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                        <  2:1143893/93‑1 (MQ=255)

                                    ccTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                        >  2:3708055/1‑93 (MQ=255)

                                     cTAAAGAGTTCAACACCTTTGTCCCGCTGGGCACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATggg                                                       >  1:1572078/1‑93 (MQ=255)

                                     cTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATggg                                                       >  2:3539326/1‑93 (MQ=255)

                                     cTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATggg                                                       >  1:3836922/1‑93 (MQ=255)

                                      tAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGt                                                      <  1:4257241/93‑1 (MQ=255)

                                      tAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGt                                                      <  1:1759081/93‑1 (MQ=255)

                                       aaaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTg                                                     >  2:2548525/1‑93 (MQ=255)

                                        aaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTgg                                                    >  2:670094/1‑93 (MQ=255)

                                         agagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGa                                                   >  1:141884/1‑93 (MQ=255)

                                         agagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGa                                                   >  1:3303948/1‑93 (MQ=255)

                                         agagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGa                                                   <  1:281675/93‑1 (MQ=255)

                                             ttCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAg                                               <  2:2636928/93‑1 (MQ=255)

                                               cAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGtt                                             >  2:1972516/1‑93 (MQ=255)

                                               cAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGtt                                             <  2:1414557/93‑1 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                            >  2:2190638/1‑93 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                            <  2:1498246/93‑1 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                         <  1:1656455/93‑1 (MQ=255)

                                                    ccTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGt                                        >  2:2037232/1‑93 (MQ=255)

                                                     cTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGtt                                       >  2:3953328/1‑93 (MQ=255)

                                                      tttGTCCCGCTGGGAACGGTGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTCTGGGTGGCTCAGTTCTGGttt                                      >  1:4282034/1‑93 (MQ=255)

                                                      tttGCCCCG‑TGGGAAGGGCGGCGTTAACCAATCCGACTTTTGGCGCTTATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTg                                     <  2:3590324/93‑1 (MQ=255)

                                                        tGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTgg                                    <  1:2307714/93‑1 (MQ=255)

                                                         gTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGt                                   >  1:1056841/1‑93 (MQ=255)

                                                          tCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTc                                  <  2:4657825/93‑1 (MQ=255)

                                                          tCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTc                                  <  2:365308/93‑1 (MQ=255)

                                                           cccGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCt                                 <  1:3924096/93‑1 (MQ=255)

                                                            ccGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTg                                >  1:2380942/1‑93 (MQ=255)

                                                             cGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGa                               >  2:2112121/1‑93 (MQ=255)

                                                             cGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGa                               >  1:1431904/1‑93 (MQ=255)

                                                             cGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGAGTGGGCGCGAATGGGTGGATCAGTTCTGGTTTGGTCTGa                               >  1:683255/1‑93 (MQ=255)

                                                              gCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaa                              >  2:2331024/1‑93 (MQ=255)

                                                               cTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTggtctggtctcaca                             >  2:192533/1‑89 (MQ=255)

                                                               cTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaag                             >  1:1540322/1‑92 (MQ=255)

                                                               cTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaaa                             <  2:110909/93‑1 (MQ=255)

                                                                tGGGAACGGCGGGGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAg                            >  1:4242381/1‑93 (MQ=255)

                                                                tGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAg                            >  1:184396/1‑93 (MQ=255)

                                                                tGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAg                            <  2:2265831/93‑1 (MQ=255)

                                                                 gggAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAgg                           >  1:706064/1‑93 (MQ=255)

                                                                 gggAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAgg                           >  2:640858/1‑93 (MQ=255)

                                                                 gggAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGGGGATCAGTTCTGGTTTGGTCTGAAAgg                           >  1:1091828/1‑93 (MQ=255)

                                                                 gggAACGGCCGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAgg                           <  1:556229/93‑1 (MQ=255)

                                                                  ggAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAggg                          <  1:406933/93‑1 (MQ=255)

                                                                   gAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGa                         <  1:2861532/93‑1 (MQ=255)

                                                                   gAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGa                         <  1:3694758/93‑1 (MQ=255)

                                                                    aaCGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGAt                        >  1:2953372/1‑93 (MQ=255)

                                                                    aaCGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGAt                        <  1:886621/93‑1 (MQ=255)

                                                                     aCGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATg                       <  1:2487673/93‑1 (MQ=255)

                                                                       ggcggcGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGa                     <  2:1768232/93‑1 (MQ=255)

                                                                        gcggcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAg                    <  2:3396156/93‑1 (MQ=255)

                                                                          ggcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCg                  >  1:1839186/1‑93 (MQ=255)

                                                                           gcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGt                 <  2:4683672/93‑1 (MQ=255)

                                                                           gcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTAGTCTGAAAGGGATGGAGCGt                 >  2:4372148/1‑93 (MQ=255)

                                                                            cgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGGATGGGCGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGtt                >  2:167416/1‑93 (MQ=255)

                                                                             tttAACCAATCCGACTTTTGGCGCTGATAGCTACTGGCGTGGTGGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTa               <  1:3224548/92‑1 (MQ=255)

                                                                             gTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTa               >  2:4343152/1‑93 (MQ=255)

                                                                              ttAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTAc              <  2:4079617/93‑1 (MQ=255)

                                                                               tAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACg             <  2:1422414/93‑1 (MQ=255)

                                                                               tAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACg             >  1:2722181/1‑93 (MQ=255)

                                                                                aaCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACgg            <  1:770591/93‑1 (MQ=255)

                                                                                aaCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACgg            >  1:3114199/1‑93 (MQ=255)

                                                                                 aCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGt           <  2:3234466/93‑1 (MQ=255)

                                                                                  ccAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGtt          <  2:1291300/93‑1 (MQ=255)

                                                                                   cAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTa         >  1:3504008/1‑93 (MQ=255)

                                                                                    aaTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTAt        <  2:1837607/93‑1 (MQ=255)

                                                                                    aaTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTAt        >  2:970196/1‑93 (MQ=255)

GCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGGCTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGTGAT  >  NC_000913/3228524‑3228706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`