BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,253,781	T→C	E171G (GAG→GGG)	yhaNM ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,253,781	0	T	C	99.1%	366.2 / ‑5.0	116	E171G (GAG→GGG)	yhaNM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (60/55); total (61/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 122 total aligned reads displayed.

TCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC  >  NC_000913/3253691‑3253870

tCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTg                                                                                        <  1:3992164/93‑1 (MQ=255)

tCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTg                                                                                        <  2:4250559/93‑1 (MQ=255)

tCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTg                                                                                        <  1:2766702/93‑1 (MQ=255)

  gccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGcc                                                                                      >  1:140221/1‑93 (MQ=255)

  gccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGcc                                                                                      <  2:2054139/93‑1 (MQ=255)

  gccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGcc                                                                                      >  1:1548490/1‑93 (MQ=255)

  gccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGcc                                                                                      <  2:2054135/93‑1 (MQ=255)

  gccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGcc                                                                                      >  2:4485666/1‑93 (MQ=255)

   ccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCca                                                                                     >  1:3967410/1‑93 (MQ=255)

    cgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcac                                                                                    >  2:2683886/1‑93 (MQ=255)

     gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcaca                                                                                   >  1:410734/1‑93 (MQ=255)

      cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacac                                                                                  <  2:2369874/93‑1 (MQ=255)

      cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacac                                                                                  >  1:2611276/1‑93 (MQ=255)

      cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTAAGCGGAGACTCTTGCTCGCCCCATGCcacac                                                                                  >  2:1508165/1‑93 (MQ=255)

       aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                                                                 <  2:3896139/93‑1 (MQ=255)

       aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                                                                 >  1:3333272/1‑93 (MQ=255)

        aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacac                                                                                >  1:2392508/1‑93 (MQ=255)

          cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGc                                                                              >  2:2786429/1‑93 (MQ=255)

           gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGGTCGCCCCCTGCCACACACGcc                                                                             <  1:841750/93‑1 (MQ=255)

           gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             >  1:129383/1‑93 (MQ=255)

            ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCct                                                                            <  1:1181333/93‑1 (MQ=255)

            ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCct                                                                            <  2:2657988/93‑1 (MQ=255)

            ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCct                                                                            >  1:4349286/1‑93 (MQ=255)

            ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTGCCTC‑CCCCCTTCCCCACACCCctg                                                                           >  1:1499171/1‑93 (MQ=255)

             gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                                                           <  2:838210/93‑1 (MQ=255)

              aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACATGCctgc                                                                          <  2:4608636/93‑1 (MQ=255)

              aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                                                          >  1:590316/1‑93 (MQ=255)

              aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                                                          <  2:1874687/93‑1 (MQ=255)

               cTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgct                                                                         >  2:3758484/1‑93 (MQ=255)

               cTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgct                                                                         >  2:4554551/1‑93 (MQ=255)

                ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        >  1:1442137/1‑93 (MQ=255)

                ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        <  2:2699665/93‑1 (MQ=255)

                 tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  1:2785261/1‑93 (MQ=255)

                 tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  1:3416340/1‑93 (MQ=255)

                      gACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaaa                                                                  >  2:792024/1‑93 (MQ=255)

                        cGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAaca                                                                <  2:4472898/93‑1 (MQ=255)

                         gAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacac                                                               <  2:3413814/93‑1 (MQ=255)

                           aCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacacca                                                             >  1:2773904/1‑93 (MQ=255)

                           aCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacacca                                                             <  1:4630659/93‑1 (MQ=255)

                            cTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacaccac                                                            <  1:291255/93‑1 (MQ=255)

                              tCAGTATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacaccacac                                                          <  1:2697290/93‑1 (MQ=255)

                                aGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAAcaccacacca                                                        >  1:3024759/1‑93 (MQ=255)

                                  gATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATc                                                      >  1:638689/1‑93 (MQ=255)

                                  gATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATc                                                      <  2:1065774/93‑1 (MQ=255)

                                  gATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATc                                                      <  2:505335/93‑1 (MQ=255)

                                   aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                     >  2:2329672/1‑93 (MQ=255)

                                   aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                     <  1:297933/93‑1 (MQ=255)

                                   aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                     >  1:2941810/1‑93 (MQ=255)

                                   aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                     >  1:10681/1‑93 (MQ=255)

                                      tcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGc                                                  >  2:4113297/1‑93 (MQ=255)

                                      ccagccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGc                                                  <  2:1432107/92‑1 (MQ=255)

                                         gccagcGTC‑GTTCTTGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc                                               >  2:4256840/1‑93 (MQ=255)

                                         gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc                                               >  1:3011430/1‑93 (MQ=255)

                                         gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc                                               >  1:4442214/1‑93 (MQ=255)

                                         gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc                                               <  1:1737659/93‑1 (MQ=255)

                                           cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             >  1:451717/1‑93 (MQ=255)

                                           cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             <  1:365736/93‑1 (MQ=255)

                                             gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCgg                                           >  1:226909/1‑92 (MQ=255)

                                             gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa                                           <  1:3216469/93‑1 (MQ=255)

                                             gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa                                           <  1:3087371/93‑1 (MQ=255)

                                             gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa                                           >  1:226910/1‑93 (MQ=255)

                                                  cgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGGAAAAACCACACCATCGTGCGCCTCGTTATg                                       >  2:1709358/2‑93 (MQ=255)

                                                     tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcac                                    <  2:1716819/93‑1 (MQ=255)

                                                       tGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACaa                                  <  1:1109983/93‑1 (MQ=255)

                                                       tGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACaa                                  >  2:1295631/1‑93 (MQ=255)

                                                       tGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACaa                                  >  1:2005551/1‑93 (MQ=255)

                                                         gAAATCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATg                                <  1:2495035/93‑1 (MQ=255)

                                                         gAAAGCCCCGTCAGCGGAGACTCTTGCTCCCCCCCTGCCACACACGCCTGCTGGGTAAACCCCACACCATCGTGCGTCTCGATATGCACAATg                                <  1:2495036/93‑1 (MQ=255)

                                                           aaGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGtt                              <  1:2598557/93‑1 (MQ=255)

                                                            aGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTg                             >  2:3447109/1‑93 (MQ=255)

                                                            aGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTg                             <  2:4558273/93‑1 (MQ=255)

                                                               cccGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                          <  1:2730570/92‑1 (MQ=255)

                                                               aCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                          >  2:4308103/1‑93 (MQ=255)

                                                                ccGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAt                         >  1:3827753/1‑93 (MQ=255)

                                                                  gTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGc                       >  1:3943794/1‑93 (MQ=255)

                                                                  gTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGc                       >  1:2266408/1‑93 (MQ=255)

                                                                   tgaGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      <  1:538936/91‑1 (MQ=255)

                                                                   tCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      >  1:930869/1‑93 (MQ=255)

                                                                      gCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCccgc                   >  1:682908/1‑93 (MQ=255)

                                                                       cGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCccgcc                  >  2:1114664/1‑93 (MQ=255)

                                                                        ggAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGCGCGTCTCGATATGCACAATGTTAGTATGTccgccg                 >  2:3454570/1‑93 (MQ=255)

                                                                         gagaCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcga                >  1:89306/1‑93 (MQ=255)

                                                                         gagaCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcga                <  2:213988/93‑1 (MQ=255)

                                                                          agaCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgac               <  1:1992594/93‑1 (MQ=255)

                                                                            aCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga             <  2:2437443/93‑1 (MQ=255)

                                                                            aCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga             <  1:3246082/93‑1 (MQ=255)

                                                                            aCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga             <  2:379994/93‑1 (MQ=255)

                                                                            aCTCGTGCTCGCCCCCTGCCACACACGCCTGCTGTGTAAACACCACACCATCGTGCGTCTCGATATGCACAATTTTGGTATGCCCGCcgacga             <  1:1984850/93‑1 (MQ=255)

                                                                               ctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg          <  2:197909/93‑1 (MQ=255)

                                                                               ctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg          <  1:4133183/93‑1 (MQ=255)

                                                                                ttGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATggg         >  1:1975727/1‑92 (MQ=255)

                                                                                ttGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGt         >  2:3142665/1‑93 (MQ=255)

                                                                                  gCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGa       <  1:1290709/93‑1 (MQ=255)

                                                                                   cTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGac      >  2:4344668/1‑93 (MQ=255)

                                                                                   cTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGac      <  2:2498957/93‑1 (MQ=255)

                                                                                    tCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGaca     >  1:2976577/1‑93 (MQ=255)

                                                                                    tCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGaca     >  2:726689/1‑93 (MQ=255)

                                                                                    tCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGaca     <  2:2907476/93‑1 (MQ=255)

                                                                                       ccccCTGGCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacacac  <  2:3983838/93‑1 (MQ=255)

                                                                                       ccccCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacacac  >  1:2384678/1‑93 (MQ=255)

TCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC  >  NC_000913/3253691‑3253870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`