BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,295,175	T→C	Q202R (CAG→CGG)	yraQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,295,175	0	T	C	99.2%	408.6 / ‑4.5	128	Q202R (CAG→CGG)	yraQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (67/59); total (68/60)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.79e-01

Only 100 of 135 total aligned reads displayed.

TCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAA  >  NC_000913/3295086‑3295267

tCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCcgcg                                                                                           >  2:279468/1‑93 (MQ=255)

 cGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGt                                                                                          >  2:2381010/1‑93 (MQ=255)

 cGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGt                                                                                          >  2:1048870/1‑93 (MQ=255)

  gTACTCCAGAAAAGCGTCCATGGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGtt                                                                                         <  2:1407225/93‑1 (MQ=255)

  gTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGtt                                                                                         <  1:3864173/93‑1 (MQ=255)

   tACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGttt                                                                                        <  1:962422/93‑1 (MQ=255)

   tACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCAGCGttt                                                                                        >  1:247049/1‑93 (MQ=255)

    aCTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTg                                                                                       <  2:3788782/93‑1 (MQ=255)

    aCTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTg                                                                                       <  1:2308127/93‑1 (MQ=255)

    aCTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTg                                                                                       <  2:4657832/93‑1 (MQ=255)

     ctgcAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAACCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACTGGTGCCCGCGTTTGc                                                                                      <  2:2675530/90‑1 (MQ=255)

     cTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGc                                                                                      <  2:4746183/93‑1 (MQ=255)

     cTCCAGAAAAGCGGCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGc                                                                                      <  1:4124224/93‑1 (MQ=255)

      tCCAGAAAGGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCg                                                                                     <  1:177941/93‑1 (MQ=255)

      tCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCg                                                                                     >  2:3413933/1‑93 (MQ=255)

        cAGAAAAGCTTCCATAGCGCCCTGCCCCAGCGTCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgt                                                                                   <  2:2412982/93‑1 (MQ=255)

         aGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgtg                                                                                  <  1:3409587/93‑1 (MQ=255)

          gAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgtgt                                                                                 <  1:2203961/93‑1 (MQ=255)

          gAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGgtgt                                                                                 >  1:2106360/1‑93 (MQ=255)

           aaaaGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGtt                                                                                <  2:1005356/93‑1 (MQ=255)

            aaaGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGttt                                                                               >  2:2690198/1‑93 (MQ=255)

               gCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCAc                                                                            <  1:3127936/93‑1 (MQ=255)

               gCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCAc                                                                            >  2:549137/1‑93 (MQ=255)

                cGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACg                                                                           <  1:4588908/93‑1 (MQ=255)

                  tCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGTCCGCGTTTGCGGTGTTTCACGCa                                                                         >  2:2557366/1‑93 (MQ=255)

                  tCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCCGTGCCCGCGTTTGCGGTGTTTCACGcc                                                                         >  2:3046485/1‑92 (MQ=255)

                     aTAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCAccc                                                                      >  1:3759639/1‑93 (MQ=255)

                     aTAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCAccc                                                                      >  2:1762169/1‑93 (MQ=255)

                     aTAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCAccc                                                                      >  2:3730196/1‑93 (MQ=255)

                      tAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCa                                                                     >  1:3884794/1‑93 (MQ=255)

                      tAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCa                                                                     <  1:3719116/93‑1 (MQ=255)

                       aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAt                                                                    >  2:1782261/1‑93 (MQ=255)

                       aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAt                                                                    >  2:127637/1‑93 (MQ=255)

                        gcgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAtt                                                                   >  1:1685130/1‑93 (MQ=255)

                         cccccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAttt                                                                  <  1:732423/91‑1 (MQ=255)

                          ccccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAtttt                                                                 <  1:93991/92‑1 (MQ=255)

                           cccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCTCCCAttttt                                                                >  1:1021535/1‑93 (MQ=255)

                           cccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAttttt                                                                <  1:2640340/93‑1 (MQ=255)

                           cccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAttttt                                                                <  2:2053820/93‑1 (MQ=255)

                            ccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCAtttttt                                                               >  1:1003383/1‑92 (MQ=255)

                            ccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTg                                                               >  1:4331588/1‑93 (MQ=255)

                            ccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGAGGTTGCGGTGTTTCACGCACCGATTTTTg                                                               >  1:4331585/1‑93 (MQ=255)

                             cTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGc                                                              >  1:3173571/1‑93 (MQ=255)

                              tGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCa                                                             >  1:932506/1‑93 (MQ=255)

                                 cccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCa                                                          >  2:3983975/1‑93 (MQ=255)

                                 cccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCa                                                          <  1:4535914/93‑1 (MQ=255)

                                  ccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGGGTTTCACGCACCCATTTTTGCAcccg                                                         >  2:789160/1‑91 (MQ=255)

                                  ccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGAACGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAg                                                         >  1:4704855/1‑93 (MQ=255)

                                   cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGc                                                        <  2:2134131/93‑1 (MQ=255)

                                    aGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCg                                                       <  2:4590499/93‑1 (MQ=255)

                                    aGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCg                                                       <  2:3440742/93‑1 (MQ=255)

                                    aGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCg                                                       <  2:3322005/93‑1 (MQ=255)

                                    aGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCg                                                       >  2:523863/1‑93 (MQ=255)

                                    aGCGGCTAAAAAACCCGCCCTGGGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCg                                                       >  2:866267/1‑93 (MQ=255)

                                      cGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTc                                                     <  1:3379003/93‑1 (MQ=255)

                                       ggCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCg                                                    >  1:708759/1‑93 (MQ=255)

                                       ggCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCg                                                    >  1:428820/1‑93 (MQ=255)

                                       ggCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCg                                                    <  1:2166942/93‑1 (MQ=255)

                                       ggCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCg                                                    <  1:2805230/93‑1 (MQ=255)

                                         cTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCa                                                  >  2:921977/1‑93 (MQ=255)

                                          tAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCACCGTCGCaa                                                 >  1:3431533/1‑93 (MQ=255)

                                             aaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCa                                              <  1:1064382/93‑1 (MQ=255)

                                                  ccGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAac                                         <  1:2463506/93‑1 (MQ=255)

                                                   cGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAaca                                        <  2:4386473/93‑1 (MQ=255)

                                                    gCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAacac                                       >  1:1612183/1‑93 (MQ=255)

                                                      ccTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCa                                     <  2:1620689/93‑1 (MQ=255)

                                                      ccTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCa                                     >  2:3887054/1‑93 (MQ=255)

                                                      ccTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCa                                     <  2:1923635/93‑1 (MQ=255)

                                                       ccgtgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCACCGTCGCAATCAGCAACACCAt                                    >  2:1096705/3‑93 (MQ=255)

                                                       cTGTGTTTGCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGGGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCac                                    >  2:4000476/1‑92 (MQ=255)

                                                        tgtgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATc                                   >  2:1160497/1‑93 (MQ=255)

                                                         gtgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCa                                  <  1:2650737/93‑1 (MQ=255)

                                                         gtgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCa                                  <  2:1047653/93‑1 (MQ=255)

                                                         gtgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCa                                  >  1:1176062/1‑93 (MQ=255)

                                                          tgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCAc                                 >  2:280872/1‑93 (MQ=255)

                                                          tgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCAc                                 <  1:3213129/93‑1 (MQ=255)

                                                          tgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCAc                                 >  1:1531718/1‑93 (MQ=255)

                                                          tgCTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCACCAACACCATCAc                                 >  1:4738460/1‑93 (MQ=255)

                                                            cTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCa                               >  2:2887435/1‑93 (MQ=255)

                                                            cTTCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCa                               >  2:3701757/1‑93 (MQ=255)

                                                             ttCCGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCac                              >  2:3219816/1‑92 (MQ=255)

                                                               ccGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGcc                            <  1:2898687/93‑1 (MQ=255)

                                                               ccGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGcc                            <  1:527872/93‑1 (MQ=255)

                                                                cGGTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGccc                           <  1:1728999/93‑1 (MQ=255)

                                                                 ggTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCg                          >  2:713529/1‑93 (MQ=255)

                                                                 ggTATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCg                          >  1:347955/1‑93 (MQ=255)

                                                                   tATGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGc                        <  1:3276394/93‑1 (MQ=255)

                                                                     tGTCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccc                      >  1:1635644/1‑92 (MQ=255)

                                                                       tCAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccacc                    <  1:101002/93‑1 (MQ=255)

                                                                        cAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccacca                   <  2:2599590/93‑1 (MQ=255)

                                                                        cAATTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccacca                   <  2:1288312/93‑1 (MQ=255)

                                                                         aaTTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAACGTCGCAATCAGCAAAACAATCACCAGCCCGGccaccaa                  >  2:3035183/1‑92 (MQ=255)

                                                                          aTTTCGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGa                 >  1:639173/1‑93 (MQ=255)

                                                                              cGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGaa             >  2:1608945/1‑93 (MQ=255)

                                                                              cGACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGaa             <  1:1156144/93‑1 (MQ=255)

                                                                               gACCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAAt            >  2:3303292/1‑93 (MQ=255)

                                                                                gccGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATc           <  2:982622/92‑1 (MQ=255)

                                                                                aCCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATc           >  2:3614092/1‑93 (MQ=255)

                                                                                aCCGGTGCCCGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATc           <  2:1168644/93‑1 (MQ=255)

                                                                                         tGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACGAGCGTCTCAAGCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCaaaa  <  2:2128065/93‑1 (MQ=255)

TCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAA  >  NC_000913/3295086‑3295267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`