BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,300,625	G→A	V172I (GTC→ATC)	yhbV →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,300,625	0	G	A	98.0%	506.3 / ‑2.6	146	V172I (GTC→ATC)	yhbV	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (67/76); total (69/77)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

GTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAA  >  NC_000913/3300536‑3300712

  ttGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTg                                                                                    <  1:3129685/92‑1 (MQ=255)

   tGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGa                                                                                   <  1:3984549/93‑1 (MQ=255)

   tGCCAGTGGAGCTTTCCCGCGACTGGCGTGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGa                                                                                   <  1:3537111/93‑1 (MQ=255)

    gCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAg                                                                                  >  1:1118694/1‑93 (MQ=255)

      cAGTGTAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCt                                                                                <  2:2289683/93‑1 (MQ=255)

      cAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATTCTGAGCt                                                                                >  1:3381327/1‑93 (MQ=255)

      cAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCt                                                                                >  1:3381324/1‑93 (MQ=255)

      cAGTGGAGCTGTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCt                                                                                <  1:2310254/93‑1 (MQ=255)

         tGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACg                                                                             <  1:3973308/93‑1 (MQ=255)

          ggAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACgg                                                                            <  2:2418393/93‑1 (MQ=255)

          ggAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGGTTGAAGTGGAAATCCTGAGCTACgg                                                                            >  2:4166866/1‑93 (MQ=255)

           gAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGt                                                                           >  1:3543034/1‑93 (MQ=255)

            aGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTc                                                                          <  1:2451971/93‑1 (MQ=255)

             gCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCa                                                                         >  1:2453604/1‑93 (MQ=255)

             gCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCa                                                                         <  2:2852742/93‑1 (MQ=255)

               tttCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATc                                                                       <  2:4415840/93‑1 (MQ=255)

                 tCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTg                                                                     <  2:3192360/93‑1 (MQ=255)

                   ccGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGcc                                                                   <  2:156654/93‑1 (MQ=255)

                       gACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTg                                                               <  1:1259373/93‑1 (MQ=255)

                       gACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTg                                                               >  1:4455226/1‑93 (MQ=255)

                        aCTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTgg                                                              >  1:764282/1‑93 (MQ=255)

                          tggctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGcc                                                            >  1:4292381/1‑93 (MQ=255)

                           ggctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCt                                                           <  1:4229805/93‑1 (MQ=255)

                           ggctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCt                                                           <  2:1390364/93‑1 (MQ=255)

                            gctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTa                                                          >  1:3192054/1‑93 (MQ=255)

                              tggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACt                                                        >  1:731625/1‑93 (MQ=255)

                               ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                       >  1:3354545/1‑93 (MQ=255)

                               ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                       >  1:683556/1‑93 (MQ=255)

                               ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                       <  2:3608824/93‑1 (MQ=255)

                               ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                       <  2:4513884/93‑1 (MQ=255)

                               ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                       >  1:2877747/1‑93 (MQ=255)

                               ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                       >  2:1956084/1‑93 (MQ=255)

                               ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                       >  2:1956082/1‑93 (MQ=255)

                                gTGAATCTGCTTAATCAGTGCGATGAGATGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGGTGGCCTACTcc                                                      <  1:289632/93‑1 (MQ=255)

                                 tGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTATAAAGCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccg                                                     <  1:941561/93‑1 (MQ=255)

                                   aaTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcc                                                   >  1:63456/1‑93 (MQ=255)

                                    aTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgccc                                                  >  1:168761/1‑93 (MQ=255)

                                    aTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgccc                                                  <  2:155943/93‑1 (MQ=255)

                                    aTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgccc                                                  <  1:467658/93‑1 (MQ=255)

                                      cTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcccgc                                                >  2:2465681/1‑93 (MQ=255)

                                       tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                               >  2:1737751/1‑93 (MQ=255)

                                       tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                               >  2:4409558/1‑93 (MQ=255)

                                       tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                               >  2:565902/1‑93 (MQ=255)

                                       tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                               >  2:3418614/1‑93 (MQ=255)

                                       tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                               >  2:3797139/1‑93 (MQ=255)

                                        gCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctg                                              >  2:1009921/1‑93 (MQ=255)

                                         cTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctgc                                             >  1:1561343/1‑93 (MQ=255)

                                          ttAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctgct                                            >  1:3837672/1‑93 (MQ=255)

                                            aaTCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCttt                                          <  1:1875804/93‑1 (MQ=255)

                                              tCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTAc                                        >  2:351124/1‑93 (MQ=255)

                                              tCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTAc                                        <  2:4721467/93‑1 (MQ=255)

                                                 gTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACcgc                                     >  2:4525672/1‑93 (MQ=255)

                                                     gATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGt                                 <  1:978738/93‑1 (MQ=255)

                                                     gATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGt                                 <  2:3979637/93‑1 (MQ=255)

                                                      aTGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGtt                                <  2:3542830/93‑1 (MQ=255)

                                                      aTGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGtt                                >  1:3620535/1‑93 (MQ=255)

                                                      aTGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGtt                                <  1:303397/93‑1 (MQ=255)

                                                       tGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTc                               <  1:1746259/93‑1 (MQ=255)

                                                       tGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTc                               <  1:4367906/93‑1 (MQ=255)

                                                        gAGCTGGGCGCTCGTACCCAGTTTGAAGTGGAAATCCTGAGCTCCGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCg                              <  2:1287354/93‑1 (MQ=255)

                                                        gAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCg                              <  1:4133381/93‑1 (MQ=255)

                                                        gAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCg                              <  2:2842829/93‑1 (MQ=255)

                                                         agttGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTTCCGCTGGACTACTCCGCCCGCTGCTTTACCGCGCGTTCgg                             <  2:156551/90‑1 (MQ=255)

                                                         aGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCGACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGTTTTACCGCGCGTTCgg                             <  2:3810063/93‑1 (MQ=255)

                                                         aGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCgg                             >  2:1646431/1‑93 (MQ=255)

                                                          gCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGa                            >  1:4382316/1‑93 (MQ=255)

                                                          gCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGa                            <  1:2045353/93‑1 (MQ=255)

                                                          gCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGa                            >  2:3760041/1‑93 (MQ=255)

                                                           cTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGaa                           <  2:4685300/93‑1 (MQ=255)

                                                             gggCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGa                         <  2:3226172/93‑1 (MQ=255)

                                                              ggCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCgg                             <  2:3479218/88‑1 (MQ=255)

                                                              ggCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCgg                             >  1:3479217/1‑88 (MQ=255)

                                                              ggCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGAc                        <  2:2628793/93‑1 (MQ=255)

                                                               gCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCAGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGAcc                       <  2:3371207/93‑1 (MQ=255)

                                                                 aTTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGCCCGc                     >  1:4674271/1‑93 (MQ=255)

                                                                 aTTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGc                     >  1:2125014/1‑93 (MQ=255)

                                                                 aTTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGc                     >  2:4630611/1‑93 (MQ=255)

                                                                  ttCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGcc                    <  2:2252059/93‑1 (MQ=255)

                                                                  ttCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGcc                    >  1:4145800/1‑93 (MQ=255)

                                                                     gTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGa                 >  2:3048466/1‑93 (MQ=255)

                                                                     ctAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGa                 <  2:1314255/92‑1 (MQ=255)

                                                                        aCCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCTCGTTCGGAAGACCGCCCGAAAg              >  1:654491/1‑93 (MQ=255)

                                                                        aCCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAg              >  2:4284037/1‑93 (MQ=255)

                                                                        aCCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAg              <  1:1886921/93‑1 (MQ=255)

                                                                         ccAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGa             >  1:254424/1‑93 (MQ=255)

                                                                         ccAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGa             >  2:4047962/1‑93 (MQ=255)

                                                                         ccAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGa             <  1:4353257/93‑1 (MQ=255)

                                                                         ccAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGa             <  2:1724872/93‑1 (MQ=255)

                                                                           aGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATg           >  2:324061/1‑93 (MQ=255)

                                                                            gTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGa          <  2:3002244/93‑1 (MQ=255)

                                                                            gTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGa          <  1:980771/93‑1 (MQ=255)

                                                                            gTTTGAAGGGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGa          <  1:3698743/93‑1 (MQ=255)

                                                                             tttGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAg         <  2:728444/93‑1 (MQ=255)

                                                                              ttGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgt        <  2:1471315/93‑1 (MQ=255)

                                                                              ttGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgt        >  2:2382871/1‑93 (MQ=255)

                                                                               tGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgtg       <  2:1609957/93‑1 (MQ=255)

                                                                               tGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgtg       >  2:3723793/1‑93 (MQ=255)

                                                                                gAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgtgt      <  1:343245/93‑1 (MQ=255)

                                                                                  aGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGa    >  2:1412920/1‑93 (MQ=255)

                                                                                   gTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGaa   >  1:2693436/1‑93 (MQ=255)

GTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAA  >  NC_000913/3300536‑3300712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`