Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,358,838	G→A	A10T (GCT→ACT)	gltF →	GltF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,358,838	0	G	A	99.0%	342.2 / ‑4.9	97	A10T (GCT→ACT)	gltF	GltF
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (95/1);  total (96/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01

GCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTAT  >  NC_000913/3358766‑3358927                                                                         |                                                                                          gCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACtt                                                                       >  1:3476183/1‑93 (MQ=255)            taAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTgcagc                                                            >  1:1783331/1‑93 (MQ=255)                aaataaatTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTc                                                        >  2:394029/1‑93 (MQ=255)                aaataaatTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTc                                                        >  2:2045571/1‑93 (MQ=255)                  ataaatTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAg                                                      >  2:2587212/1‑93 (MQ=255)                  ataaatTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAg                                                      >  2:3311019/1‑93 (MQ=255)                  ataaatTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAg                                                      >  2:2587213/1‑93 (MQ=255)                   taaatTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGt                                                     >  1:88258/1‑93 (MQ=255)                      atTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGcc                                                  >  1:3246047/1‑93 (MQ=255)                      atTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGcc                                                  >  1:3374846/1‑93 (MQ=255)                       tttGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCa                                                 >  1:781035/1‑93 (MQ=255)                       tttGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCa                                                 >  1:1582215/1‑93 (MQ=255)                       tttGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCa                                                 >  1:3552598/1‑93 (MQ=255)                       tttGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCa                                                 >  2:4201711/1‑93 (MQ=255)                        ttGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCAt                                                >  1:1755005/1‑93 (MQ=255)                        ttGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCAt                                                >  2:2990793/1‑93 (MQ=255)                          gTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATgg                                              >  1:4578652/1‑93 (MQ=255)                          gTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATgg                                              >  2:2209008/1‑93 (MQ=255)                           ttattaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGc                                             >  2:2152863/1‑93 (MQ=255)                            tattaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCa                                            >  2:2187071/1‑93 (MQ=255)                             attaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCaa                                           >  1:4138240/1‑93 (MQ=255)                             attaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCAAAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCac                                           >  2:1140927/1‑92 (MQ=255)                              ttaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAAc                                          >  1:3615060/1‑93 (MQ=255)                              ttaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAAc                                          >  1:4444179/1‑93 (MQ=255)                              ttaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAAc                                          >  1:1666725/1‑93 (MQ=255)                               taAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACa                                         >  1:2352243/1‑93 (MQ=255)                                aaGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAg                                        >  2:3679280/1‑93 (MQ=255)                                aaGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAg                                        >  2:827211/1‑93 (MQ=255)                                aaGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAg                                        >  2:827210/1‑93 (MQ=255)                                aaGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAg                                        >  2:716315/1‑93 (MQ=255)                                 aGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGa                                       >  1:1484023/1‑93 (MQ=255)                                 aGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGa                                       >  1:1935227/1‑93 (MQ=255)                                  ggATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAt                                      >  1:2783114/1‑93 (MQ=255)                                  ggATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAt                                      >  2:2361635/1‑93 (MQ=255)                                  ggATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAt                                      >  2:4403760/1‑93 (MQ=255)                                  ggATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAt                                      >  2:4351667/1‑93 (MQ=255)                                  ggATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAt                                      >  2:293430/1‑93 (MQ=255)                                   gATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAtt                                     >  1:218960/1‑93 (MQ=255)                                    atatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTc                                    >  2:4621992/1‑93 (MQ=255)                                    atatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTc                                    >  2:3693716/1‑93 (MQ=255)                                     tatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCa                                   >  1:2093734/1‑93 (MQ=255)                                     tatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCa                                   >  2:4053343/1‑93 (MQ=255)                                     tatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCa                                   >  2:3107170/1‑93 (MQ=255)                                     tatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCa                                   >  1:850094/1‑93 (MQ=255)                                     tatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCa                                   >  2:1517555/1‑93 (MQ=255)                                      atGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCaa                                  >  1:2428361/1‑93 (MQ=255)                                       tGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAGCAGATTCAAc                                 >  1:1290462/1‑93 (MQ=255)                                       tGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAAc                                 >  2:3495004/1‑93 (MQ=255)                                       tGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAAc                                 >  2:3530262/1‑93 (MQ=255)                                       tGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAAc                                 >  1:2908741/1‑93 (MQ=255)                                        gTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACt                                >  1:131388/1‑93 (MQ=255)                                         ttCATATGTTTTTCAAAAAGAACCTCACAGCAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTg                               >  2:3361113/1‑93 (MQ=255)                                          tCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGa                              >  2:1782851/1‑93 (MQ=255)                                          tCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGa                              >  2:1782720/1‑93 (MQ=255)                                           cATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGAt                             >  1:1615519/1‑93 (MQ=255)                                           cATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGAt                             >  1:2972329/1‑93 (MQ=255)                                           cATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGCAAACAGATTAAACtaaa                             >  1:1615518/1‑90 (MQ=255)                                            atatGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATa                            >  2:4506516/1‑93 (MQ=255)                                            atatGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATa                            >  1:3430360/1‑93 (MQ=255)                                             tatGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATAc                           >  2:1956942/1‑93 (MQ=255)                                             tatGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATAc                           >  1:3161488/1‑93 (MQ=255)                                             tatGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATAc                           >  1:4384187/1‑93 (MQ=255)                                              atGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACa                          >  2:2064948/1‑93 (MQ=255)                                               tGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAg                         >  2:2664403/1‑93 (MQ=255)                                               tGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAg                         >  1:4459121/1‑93 (MQ=255)                                                 tttttCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGat                       >  1:1393801/1‑92 (MQ=255)                                                 tttttCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGaa                       >  2:2156199/1‑93 (MQ=255)                                                  ttttCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCACCAGTTTAAACTGATAAAGAAt                      >  1:723484/1‑93 (MQ=255)                                                   tttCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAAtt                     >  2:2485178/1‑93 (MQ=255)                                                   tttCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAAtt                     >  2:717187/1‑93 (MQ=255)                                                       aaaaaGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACt                 >  1:2347243/1‑93 (MQ=255)                                                        aaaaGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTa                >  2:4324599/1‑93 (MQ=255)                                                        aaaaGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTa                >  1:886446/1‑93 (MQ=255)                                                         aaaGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTat               >  2:583146/1‑93 (MQ=255)                                                         aaaGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTat               >  2:583145/1‑93 (MQ=255)                                                          aaGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatc              >  1:1914078/1‑93 (MQ=255)                                                           aGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatca             >  2:3400710/1‑93 (MQ=255)                                                            gAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcat            >  1:2242281/1‑93 (MQ=255)                                                            gAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcat            >  2:1067865/1‑93 (MQ=255)                                                            gAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcat            >  1:1732601/1‑93 (MQ=255)                                                            gAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcat            >  2:2134625/1‑93 (MQ=255)                                                             aaCCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcatc           <  1:3548871/93‑1 (MQ=255)                                                              aCCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCg          >  1:2021865/1‑93 (MQ=255)                                                               ccTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCgg         >  1:4723989/1‑93 (MQ=255)                                                                cTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGc        >  1:2551078/1‑93 (MQ=255)                                                                cTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGc        >  1:1655232/1‑93 (MQ=255)                                                                 tCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCg       >  2:4326951/1‑93 (MQ=255)                                                                 tCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCg       >  1:2894241/1‑93 (MQ=255)                                                                 tCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCg       >  2:4717423/1‑93 (MQ=255)                                                                 tCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCg       >  2:2896258/1‑93 (MQ=255)                                                                 tCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCg       >  2:1192302/1‑93 (MQ=255)                                                                 tCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCg       >  2:1897710/1‑93 (MQ=255)                                                                   acaacaACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAg     >  1:3202088/1‑93 (MQ=255)                                                                   acaacaACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAg     >  2:1946609/1‑93 (MQ=255)                                                                    caacaaCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGt    >  2:370219/1‑93 (MQ=255)                                                                    caacaaCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGt    >  1:3701786/1‑93 (MQ=255)                                                                      acaACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGtat  >  2:4015110/1‑93 (MQ=255)                                                                      acaACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGtat  >  2:336132/1‑93 (MQ=255)                                                                      acaACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGtat  >  2:2790781/1‑93 (MQ=255)                                                                         |                                                                                          GCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTAT  >  NC_000913/3358766‑3358927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑