BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,359,299	T→C	G163G (GGT→GGC)	gltF →	GltF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,359,299	0	T	C	98.5%	614.3 / ‑1.6	196	G163G (GGT→GGC)	gltF	GltF
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (98/94); total (100/96)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01

Only 100 of 199 total aligned reads displayed.

AGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCT  >  NC_000913/3359209‑3359388

 ggTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGaa                                                                                        >  1:2570733/1‑93 (MQ=255)

   ttgtGCGCCTAATGGACAAAAGATTGGCGCTGCATCCATTGGTAATGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAAt                                                                                      <  2:885624/90‑1 (MQ=255)

   ttCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAAt                                                                                      <  2:3932164/93‑1 (MQ=255)

   ttCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAAt                                                                                      <  2:2844817/93‑1 (MQ=255)

      tGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTcc                                                                                   >  2:2077886/1‑93 (MQ=255)

      tGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTcc                                                                                   <  1:742904/93‑1 (MQ=255)

      tGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTcc                                                                                   >  2:1013863/1‑93 (MQ=255)

      tGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTcc                                                                                   >  2:2055436/1‑93 (MQ=255)

      tGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTcc                                                                                   >  1:3882109/1‑93 (MQ=255)

      tGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTcc                                                                                   <  2:4655085/93‑1 (MQ=255)

       gcgcCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCt                                                                                  <  2:2398726/93‑1 (MQ=255)

        cgcCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTg                                                                                 >  2:3204591/1‑93 (MQ=255)

         gcCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGt                                                                                >  2:3700775/1‑93 (MQ=255)

         gcCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGt                                                                                <  1:3564502/93‑1 (MQ=255)

          ccTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTc                                                                               <  2:2511003/93‑1 (MQ=255)

            tAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGa                                                                             >  1:4336729/1‑93 (MQ=255)

            tAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGa                                                                             >  2:2176297/1‑93 (MQ=255)

              aTGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGAcc                                                                           >  1:2401387/1‑93 (MQ=255)

               tGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCt                                                                          >  1:1829904/1‑93 (MQ=255)

                ggACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATCCAGGCGAAATTCCTGTCGACCTg                                                                         >  1:1262339/1‑93 (MQ=255)

                ggACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTg                                                                         <  1:464745/93‑1 (MQ=255)

                ggACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTg                                                                         <  1:4222421/93‑1 (MQ=255)

                 gACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGa                                                                        >  2:449279/1‑93 (MQ=255)

                  aCAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGAt                                                                       <  1:2270059/93‑1 (MQ=255)

                  aCAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGAt                                                                       <  2:1613990/93‑1 (MQ=255)

                  aCAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGAt                                                                       <  1:1488218/93‑1 (MQ=255)

                   cAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGAtt                                                                      <  2:1782252/93‑1 (MQ=255)

                    aaaaaaTTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTc                                                                     >  1:1722537/1‑93 (MQ=255)

                    aaaaaaTTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTc                                                                     >  1:709173/1‑93 (MQ=255)

                      aaaaTTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCaa                                                                   <  1:199696/93‑1 (MQ=255)

                      aaaaTTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCaa                                                                   >  1:4391410/1‑93 (MQ=255)

                       aaaTTGGCGCGGCATCCATTCGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACAATACAGGCGAAATTCCTGTCGACCTGATTCaaa                                                                  <  1:3823772/93‑1 (MQ=255)

                        aaTTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAAc                                                                 <  2:293113/93‑1 (MQ=255)

                         aTTGGCGCTGCATTCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACt                                                                <  1:1077037/93‑1 (MQ=255)

                         aTTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACt                                                                >  2:2821915/1‑93 (MQ=255)

                         aTTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACt                                                                <  2:2291666/93‑1 (MQ=255)

                          ttGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTg                                                               >  2:1824186/1‑93 (MQ=255)

                           tGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGa                                                              <  2:292284/93‑1 (MQ=255)

                           tGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGa                                                              <  1:3115296/93‑1 (MQ=255)

                            ggCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGAc                                                             <  2:4276684/93‑1 (MQ=255)

                             gcgcTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGAcc                                                            >  1:1784572/1‑93 (MQ=255)

                                cTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACt                                                         <  1:1190652/93‑1 (MQ=255)

                                cTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACt                                                         >  1:945963/1‑93 (MQ=255)

                                 tGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTg                                                        >  1:1380320/1‑93 (MQ=255)

                                 tGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTAACCACTg                                                        >  1:1472458/1‑93 (MQ=255)

                                     tCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTca                                                    >  2:1317160/1‑93 (MQ=255)

                                      ccATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTcag                                                   <  2:1909300/93‑1 (MQ=255)

                                       cATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTcagc                                                  <  2:4554587/93‑1 (MQ=255)

                                       cATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTcagc                                                  >  2:35246/1‑93 (MQ=255)

                                       cATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTcagc                                                  >  1:1112839/1‑93 (MQ=255)

                                        aTTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTcagca                                                 >  1:721386/1‑93 (MQ=255)

                                        aTTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTcagca                                                 >  1:1650151/1‑93 (MQ=255)

                                        aTTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTcagca                                                 >  1:3170614/1‑93 (MQ=255)

                                         ttGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTcagcag                                                >  1:4698340/1‑93 (MQ=255)

                                         ttGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTcagcag                                                >  2:261944/1‑93 (MQ=255)

                                             tATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGAc                                            <  1:2864178/93‑1 (MQ=255)

                                                  aCAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCca                                       >  2:2699884/1‑93 (MQ=255)

                                                  aCAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCca                                       <  2:3591939/93‑1 (MQ=255)

                                                    aGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCcaca                                     <  1:2567271/93‑1 (MQ=255)

                                                     ggTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACAt                                    <  1:3789417/93‑1 (MQ=255)

                                                     ggTCTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACAt                                    >  2:2772756/1‑93 (MQ=255)

                                                       gcTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATgg                                  <  2:4007265/92‑1 (MQ=255)

                                                        cTAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGa                                 <  2:1364831/93‑1 (MQ=255)

                                                         tAATGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGaa                                >  2:625078/1‑93 (MQ=255)

                                                          aaTGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGaaa                               <  1:581438/93‑1 (MQ=255)

                                                           aTGGTGGGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAg                              >  2:1497762/1‑93 (MQ=255)

                                                            tggtggGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGc                             >  2:751844/1‑93 (MQ=255)

                                                             ggtggGATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGcc                            <  2:3047089/93‑1 (MQ=255)

                                                                gggATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCa                         >  2:1160454/1‑93 (MQ=255)

                                                                 ggATTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCAt                        <  2:3022311/93‑1 (MQ=255)

                                                                   aTTCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCt                      <  1:4516497/93‑1 (MQ=255)

                                                                     tCACGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTaa                    >  1:1290328/1‑93 (MQ=255)

                                                                       aCGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTACTg                  >  1:2477384/1‑93 (MQ=255)

                                                                       aCGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATg                  <  2:4319526/93‑1 (MQ=255)

                                                                       aCGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATg                  >  1:1045874/1‑93 (MQ=255)

                                                                       aCGCGGCGGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATg                  <  1:4674639/93‑1 (MQ=255)

                                                                         gcggcgGACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGgc                <  2:3335607/93‑1 (MQ=255)

                                                                           ggcggACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGgcgc              >  2:3450799/1‑93 (MQ=255)

                                                                            gcggACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGGCAGCAGCGACACCCACATGGAAAGCCTCATCTAATGCCGCa             >  2:2131493/1‑93 (MQ=255)

                                                                             cggACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCAt            >  1:1386492/1‑93 (MQ=255)

                                                                             cggACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTAACCACTGGTCAGGAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGGAt            >  1:2514891/1‑93 (MQ=255)

                                                                              ggACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCAtt           <  1:1751589/93‑1 (MQ=255)

                                                                              ggACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCAtt           <  1:3688800/93‑1 (MQ=255)

                                                                               gACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTc          <  1:1102855/93‑1 (MQ=255)

                                                                               gACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTc          >  2:4141516/1‑93 (MQ=255)

                                                                               gACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTc          <  2:3308822/93‑1 (MQ=255)

                                                                               gACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTc          <  2:2256668/93‑1 (MQ=255)

                                                                               gACGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTc          <  1:3016693/93‑1 (MQ=255)

                                                                                aCGATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCt         <  1:908951/93‑1 (MQ=255)

                                                                                aCGAGACAGGCGAAATTCCTGCCGACCTGAGTCAATCGGACCACTGGCAAGCAGCGCCCCTCACATGGAAAGCCTCATGTAATGGCGCATTCt         <  2:4384494/93‑1 (MQ=255)

                                                                                  gATACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGc       <  2:1150544/93‑1 (MQ=255)

                                                                                   aTACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCt      >  1:3729641/1‑93 (MQ=255)

                                                                                    tACAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTc     <  1:2440286/93‑1 (MQ=255)

                                                                                     aCAGGCGAAATTCCTGTCGACCTGATTCAAACTTACCACTGGCCAGCATCGATCCCCACATGGAAAGCCTCACCTCATGGGGTATTCTGCTcc    >  2:2521833/1‑92 (MQ=255)

                                                                                     aCAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCg    <  1:2195616/93‑1 (MQ=255)

                                                                                      cAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGc   >  1:1586182/1‑93 (MQ=255)

                                                                                      cAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGc   <  2:389266/93‑1 (MQ=255)

                                                                                      cAGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGc   >  2:1525576/1‑93 (MQ=255)

                                                                                       aGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCt  <  1:689198/93‑1 (MQ=255)

                                                                                       aGGCGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCt  >  1:3490017/1‑93 (MQ=255)

AGGTTCTGCGCCTAATGGACAAAAAATTGGCGCTGCATCCATTGGTATTGACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCT  >  NC_000913/3359209‑3359388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`