BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,383,369	C→T	T83M (ACG→ATG)	yhcN →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,383,369	0	C	T	99.1%	734.3 / ‑5.6	211	T83M (ACG→ATG)	yhcN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (97/111); total (98/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

CAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGA  >  NC_000913/3383279‑3383459

 aaTCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGc                                                                                         >  1:1012426/1‑93 (MQ=255)

  aTCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGcc                                                                                        >  2:2553050/1‑93 (MQ=255)

  aTCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGcc                                                                                        <  1:2633607/93‑1 (MQ=255)

   tCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCt                                                                                       >  2:1902219/1‑93 (MQ=255)

   tCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCt                                                                                       <  1:4420217/93‑1 (MQ=255)

   tCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCt                                                                                       >  2:694123/1‑93 (MQ=255)

     gggACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTAc                                                                                     >  1:1107924/1‑93 (MQ=255)

     gggACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTAc                                                                                     >  1:3660638/1‑93 (MQ=255)

       gACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCa                                                                                   <  2:2204098/93‑1 (MQ=255)

       gACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCa                                                                                   <  2:497958/93‑1 (MQ=255)

        aCCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAg                                                                                  <  2:2612709/93‑1 (MQ=255)

        aCCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAg                                                                                  <  2:832846/93‑1 (MQ=255)

             aTCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTat                                                                             >  2:4358947/1‑92 (MQ=255)

             aTCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTAc                                                                             <  1:3158095/93‑1 (MQ=255)

             aTCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTAc                                                                             <  1:2578658/93‑1 (MQ=255)

              tCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACt                                                                            <  2:512486/93‑1 (MQ=255)

                cGTAGGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCCGAATCCTGa                                                                          <  2:4196203/93‑1 (MQ=255)

                cGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGa                                                                          >  2:3032662/1‑93 (MQ=255)

                cGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGa                                                                          <  1:3719016/93‑1 (MQ=255)

                   aaGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGc                                                                       <  1:3782933/93‑1 (MQ=255)

                   aaGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGc                                                                       >  2:2844292/1‑93 (MQ=255)

                    aGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCt                                                                      <  2:101749/93‑1 (MQ=255)

                    aGTGGTGTGGCGTCGTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCt                                                                      <  1:1216131/93‑1 (MQ=255)

                       tgtgtGGCATCTTCGCCAATGGATATGAGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTTGt                                                                   <  1:167376/92‑1 (MQ=255)

                       ggtgTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGt                                                                   <  1:2485843/93‑1 (MQ=255)

                       ggtgTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGt                                                                   >  1:426585/1‑93 (MQ=255)

                       ggtgTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGt                                                                   <  1:4674969/93‑1 (MQ=255)

                       ggtgTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGt                                                                   >  2:4497290/1‑93 (MQ=255)

                       ggtgTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGt                                                                   <  2:2521038/93‑1 (MQ=255)

                        gtgtGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTa                                                                  <  1:1335225/93‑1 (MQ=255)

                        gtgtGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTa                                                                  <  1:2427115/93‑1 (MQ=255)

                            ggCGTTTTCCCCAATGGATATGCGTGAAATGCTGCACAAAAAAGAGGAAGAGAAAGGCCCAACGGCCTACCAGATTACTGAAGCTCGTAGCgg                                                              <  2:3807052/93‑1 (MQ=255)

                             gCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGt                                                             <  2:3940253/93‑1 (MQ=255)

                               gTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGa                                                           >  1:3319627/1‑93 (MQ=255)

                               gTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGa                                                           <  2:2666099/93‑1 (MQ=255)

                               gTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGa                                                           >  2:3127444/1‑93 (MQ=255)

                                  ttCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGacac                                                        <  1:3128626/93‑1 (MQ=255)

                                  ttCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGacac                                                        >  2:3256341/1‑93 (MQ=255)

                                   tCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACAcc                                                       >  1:4616917/1‑93 (MQ=255)

                                   tCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACAcc                                                       <  2:1787684/93‑1 (MQ=255)

                                   tCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACAcc                                                       <  2:1835417/93‑1 (MQ=255)

                                    cGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCt                                                      >  1:1483826/1‑93 (MQ=255)

                                     gCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTg                                                     <  2:2706451/93‑1 (MQ=255)

                                     gCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTg                                                     <  1:2567266/93‑1 (MQ=255)

                                       cAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGc                                                   >  1:356813/1‑93 (MQ=255)

                                       cAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGc                                                   >  1:2768982/1‑93 (MQ=255)

                                        aaTGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCa                                                  >  2:134674/1‑93 (MQ=255)

                                        aaTGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCa                                                  >  2:3009835/1‑93 (MQ=255)

                                        aaTGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCa                                                  >  2:2342203/1‑93 (MQ=255)

                                        aaTGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACATGGCa                                                  >  1:1257248/1‑93 (MQ=255)

                                          tGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACg                                                >  1:856210/1‑93 (MQ=255)

                                          tGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACg                                                >  2:2474743/1‑93 (MQ=255)

                                           ggATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGc                                               <  2:3693261/93‑1 (MQ=255)

                                           ggATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGc                                               <  1:2840421/93‑1 (MQ=255)

                                           ggATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACAACTGGCACGc                                               >  2:279785/1‑93 (MQ=255)

                                            gATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCt                                              >  2:762685/1‑93 (MQ=255)

                                             atatGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTa                                             <  2:3519421/93‑1 (MQ=255)

                                              tatGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTAc                                            <  1:4439595/93‑1 (MQ=255)

                                              tatGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTAc                                            >  2:3622741/1‑93 (MQ=255)

                                                tGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACgg                                          <  2:3740631/93‑1 (MQ=255)

                                                  cGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCt                                        <  2:832998/93‑1 (MQ=255)

                                                     gAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGaa                                     >  1:557166/1‑93 (MQ=255)

                                                      aaaTGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAAc                                    <  1:3322637/93‑1 (MQ=255)

                                                      aaaTGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAAc                                    >  1:1550392/1‑93 (MQ=255)

                                                      aaaTGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAAc                                    >  2:4423318/1‑93 (MQ=255)

                                                        aTGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTg                                  <  1:1347520/93‑1 (MQ=255)

                                                        aTGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTg                                  >  2:3627813/1‑93 (MQ=255)

                                                         tGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGt                                 <  2:4595389/93‑1 (MQ=255)

                                                         tGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGt                                 <  2:2511271/93‑1 (MQ=255)

                                                          gCTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTa                                >  2:916219/1‑93 (MQ=255)

                                                           cTGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTAc                               <  1:3013077/93‑1 (MQ=255)

                                                            tGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGGAGTGACACCTGGCACGCGACGGATGAACTGTACa                              >  1:4177451/1‑93 (MQ=255)

                                                            tGAACAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACa                              >  1:1919962/1‑93 (MQ=255)

                                                              aaCAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACaaa                            <  2:1283737/93‑1 (MQ=255)

                                                               aCAAAAAAGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAAt                           >  2:1631071/1‑93 (MQ=255)

                                                               aCAAAAAAGCAGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAAt                           >  2:3057686/1‑93 (MQ=255)

                                                                   aaaaGCGGAAGAGAAAGGCGCAATGGCCTACCATATTACTGATGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAAc                       <  2:1074585/93‑1 (MQ=255)

                                                                   aaaaGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAAc                       <  1:4456155/93‑1 (MQ=255)

                                                                    aaaGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACCCCTGGCACGCTACGGCTGAACTGTACAAATAAAcc                      <  2:4059863/93‑1 (MQ=255)

                                                                    aaaGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAAcc                      >  1:1721807/1‑93 (MQ=255)

                                                                     aaGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCCCGCTACGGCTGAACTGTACAAATAAAccc                     <  2:1422838/93‑1 (MQ=255)

                                                                     aaGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAAccc                     >  1:3188283/1‑93 (MQ=255)

                                                                     aaGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAAccc                     <  2:1246080/93‑1 (MQ=255)

                                                                      aGCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCt                    <  1:1424408/93‑1 (MQ=255)

                                                                       gCGGAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTc                   >  1:909170/1‑93 (MQ=255)

                                                                         ggAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCAt                 >  1:770751/1‑93 (MQ=255)

                                                                         ggAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCAt                 <  1:2058316/93‑1 (MQ=255)

                                                                          gAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATc                <  1:4253980/93‑1 (MQ=255)

                                                                          gAAGAGAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATc                <  1:3969710/93‑1 (MQ=255)

                                                                            agagAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACCCCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGt              <  1:3946723/93‑1 (MQ=255)

                                                                            agagAAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGt              >  2:203478/1‑93 (MQ=255)

                                                                              agaAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCt            <  1:1636624/93‑1 (MQ=255)

                                                                              agaAAGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCt            <  1:2274579/93‑1 (MQ=255)

                                                                                 aaGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGt         <  1:1190466/93‑1 (MQ=255)

                                                                                 aaGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGt         >  2:3362682/1‑93 (MQ=255)

                                                                                 aaGGCGCAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGt         <  2:2279187/93‑1 (MQ=255)

                                                                                     cgcAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCcga     <  1:3862346/93‑1 (MQ=255)

                                                                                     cgcAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCcga     <  2:2494697/93‑1 (MQ=255)

                                                                                      gcAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCcgac    >  2:4159912/1‑93 (MQ=255)

                                                                                       cAATGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCcgacg   <  2:4533980/93‑1 (MQ=255)

CAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGA  >  NC_000913/3383279‑3383459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`