BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,393,906	T→C	intergenic (‑4243/+57)	tldD ← / ← cafA	TldD/CafA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,393,906	0	T	C	96.7%	563.9 / 4.9	183	intergenic (‑4243/+57)	tldD/cafA	TldD/CafA
Reads supporting (aligned to +/- strand): ref base T (3/3); new base C (90/87); total (93/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 190 total aligned reads displayed.

CGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCTGGTTAT  >  NC_000913/3393816‑3393997

   cggCCGCTAACCAGCAGGGCAGAGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCt                                                                                        <  2:3583810/92‑1 (MQ=255)

   aGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCt                                                                                        <  1:1832014/93‑1 (MQ=255)

   aGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGCAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCt                                                                                        >  1:765803/1‑93 (MQ=255)

    ggCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTg                                                                                       >  2:1034983/1‑93 (MQ=255)

    ggCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTg                                                                                       >  2:4686737/1‑93 (MQ=255)

    ggCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTg                                                                                       >  1:1742803/1‑93 (MQ=255)

       cGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTa                                                                                    >  2:4517694/1‑93 (MQ=255)

         cTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTaaa                                                                                  <  1:3262032/93‑1 (MQ=255)

          tAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTaaaa                                                                                 >  1:4107420/1‑93 (MQ=255)

           aaCCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTaaaaa                                                                                >  2:4478595/1‑93 (MQ=255)

            aCCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTGCTAAAAAt                                                                               >  1:3782938/1‑93 (MQ=255)

                 cagGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGAc                                                                          <  2:422675/93‑1 (MQ=255)

                  agGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACt                                                                         >  1:3741810/1‑93 (MQ=255)

                   gggCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTc                                                                        <  1:1753398/93‑1 (MQ=255)

                   gggCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTc                                                                        >  2:3081044/1‑93 (MQ=255)

                     gCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCaa                                                                      >  2:701946/1‑93 (MQ=255)

                      cAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCaca                                                                     >  1:3603141/1‑91 (MQ=255)

                      cAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCaaa                                                                     <  2:282680/93‑1 (MQ=255)

                        gCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCaaaaa                                                                   >  1:2436886/1‑93 (MQ=255)

                        gCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCaaaaa                                                                   >  1:1954090/1‑93 (MQ=255)

                        gCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCaaaaa                                                                   >  2:574690/1‑93 (MQ=255)

                        gCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCaaaaa                                                                   >  1:488918/1‑93 (MQ=255)

                           aTCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAAccc                                                                >  2:2970521/1‑93 (MQ=255)

                           aTCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAAccc                                                                <  2:540541/93‑1 (MQ=255)

                           aTCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAAccc                                                                <  2:2035811/93‑1 (MQ=255)

                            tcccaACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCt                                                               <  1:3956801/90‑1 (MQ=255)

                            tCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCt                                                               <  2:2838460/93‑1 (MQ=255)

                            tCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCt                                                               <  1:4140611/93‑1 (MQ=255)

                            tCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCt                                                               <  2:1215009/93‑1 (MQ=255)

                             cacaACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCtt                                                              <  2:2350674/93‑1 (MQ=255)

                             cacaACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCtt                                                              <  2:3231269/93‑1 (MQ=255)

                              acaACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCttt                                                             <  2:2457045/93‑1 (MQ=255)

                              acaACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCttt                                                             <  2:2059586/93‑1 (MQ=255)

                                 aCGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAATATGTGGCTCAAAAACACTTTGcc                                                          >  2:2589571/1‑93 (MQ=255)

                                  cGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCg                                                         >  2:2484136/1‑93 (MQ=255)

                                    aGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAATAATGTGACTCAAAAACCCTTTGCCGGa                                                       >  1:4197984/1‑93 (MQ=255)

                                    aGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGa                                                       <  2:1534659/93‑1 (MQ=255)

                                    aGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGa                                                       <  2:3841040/93‑1 (MQ=255)

                                      cgcgGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATg                                                     <  2:3668178/93‑1 (MQ=255)

                                            tCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGcc                                               >  2:3179062/1‑93 (MQ=255)

                                            tCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGcc                                               >  2:472333/1‑93 (MQ=255)

                                            tCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGcc                                               >  1:3127022/1‑93 (MQ=255)

                                             ccAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGccc                                              >  2:4255599/1‑93 (MQ=255)

                                              cAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCa                                             >  1:451548/1‑93 (MQ=255)

                                              cAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCa                                             >  2:3271117/1‑93 (MQ=255)

                                              cAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCa                                             <  1:975258/93‑1 (MQ=255)

                                               aGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAg                                            >  2:438792/1‑93 (MQ=255)

                                                 tAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCa                                          <  2:1986288/93‑1 (MQ=255)

                                                 tAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCa                                          <  1:2972107/93‑1 (MQ=255)

                                                 tAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCa                                          <  2:3376782/93‑1 (MQ=255)

                                                  aaGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCAt                                         >  1:1191217/1‑93 (MQ=255)

                                                  aaGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTAAAAAACCCTTTGCCGGATGGCGGCCCAGCAt                                         <  1:2678966/93‑1 (MQ=255)

                                                   aGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATc                                        <  1:1533491/93‑1 (MQ=255)

                                                   aGCAGTAAAATCCCCGGCAATCGCCTCACCCGGCACTCCCTGGCTGCTAAAAATGTGACTCAAAAACCCNTTGCCGGATGGCGGCCCAGCATc                                        >  1:2225727/1‑93 (MQ=255)

                                                    gCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCt                                       >  2:1233274/1‑93 (MQ=255)

                                                    gCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCt                                       >  1:3391091/1‑93 (MQ=255)

                                                     cAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTGCTAAAAATGTGACTCAAAACCCCTTTGCCGGATGGCGGCCCAGCATCTg                                      <  2:2909172/93‑1 (MQ=255)

                                                     cAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTg                                      >  1:1124485/1‑93 (MQ=255)

                                                      aGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGt                                     <  1:1160430/93‑1 (MQ=255)

                                                       gTAAAATCCCCGGCAATCGCCTCCCCGGTCACTCCCTTTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGtt                                    <  2:1430042/93‑1 (MQ=255)

                                                       gTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGtt                                    <  1:3030366/93‑1 (MQ=255)

                                                       gTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGtt                                    <  2:1307253/93‑1 (MQ=255)

                                                            aTCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTAcat                               <  2:3462952/93‑1 (MQ=255)

                                                            aTCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTAcat                               >  2:2859158/1‑93 (MQ=255)

                                                             tCCCCGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTAcatc                              <  1:1349398/93‑1 (MQ=255)

                                                             accccGGCAACCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTAcatc                              <  1:802588/92‑1 (MQ=255)

                                                               cccGGCAATCGCCTCACACGTTACTCCCTGTCTGCTAAAAAAGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTTTTTAcatcat                            <  2:3057952/93‑1 (MQ=255)

                                                                ccGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCAtt                           >  1:3862462/1‑93 (MQ=255)

                                                                ccGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCAtt                           <  1:227376/93‑1 (MQ=255)

                                                                 cGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTa                          >  1:1122947/1‑93 (MQ=255)

                                                                 cGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTa                          <  2:774965/93‑1 (MQ=255)

                                                                 cGGCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTa                          >  1:3003540/1‑93 (MQ=255)

                                                                  ggCAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTac                         <  1:3208543/93‑1 (MQ=255)

                                                                    cAATCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTacga                       <  1:4337516/93‑1 (MQ=255)

                                                                     aaTCGCCTCACCCTTCACTCCCTTTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTacgac                      >  2:1639684/1‑93 (MQ=255)

                                                                     aaTCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTacgac                      <  1:1685447/93‑1 (MQ=255)

                                                                     aaTCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTacgac                      >  1:2079057/1‑93 (MQ=255)

                                                                     aaTCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTacgac                      >  1:4240112/1‑93 (MQ=255)

                                                                      aTCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTacgacg                     >  2:1849819/1‑93 (MQ=255)

                                                                       tCGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGt                    >  2:4593464/1‑93 (MQ=255)

                                                                        cGCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTc                   <  2:2182938/93‑1 (MQ=255)

                                                                         gCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCa                  <  1:672431/93‑1 (MQ=255)

                                                                         gCCTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCa                  <  1:2818161/93‑1 (MQ=255)

                                                                           cTCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCaaa                >  1:4423247/1‑93 (MQ=255)

                                                                            tCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAAc               >  2:2336677/1‑93 (MQ=255)

                                                                            tCACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAAc               <  2:4035550/93‑1 (MQ=255)

                                                                             cACCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGACCAGCATCTGTTTACATCATTACGACGTCAAACt              >  2:1180390/1‑93 (MQ=255)

                                                                              ccccGTCCCTCCCTGTCGGCTAAAAATGTGCCTCAAAACCCCTTTGCCGAATGGCGCCCCAGCATCTGTTTACATCATTACGACGTCAAACTg             <  1:3396828/92‑1 (MQ=255)

                                                                              aCCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTg             >  2:4185461/1‑93 (MQ=255)

                                                                              aCCCGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTg             >  1:4359239/1‑93 (MQ=255)

                                                                               cccGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGc            <  2:3895935/93‑1 (MQ=255)

                                                                                ccGTCACTCCCTGTCTGCTAAAAATGTGACTCAAAAGCCCTTTGCCGGATGGCGACCCAGCATCTGTTTACATCATTACGACGTCAAACTGCt           <  1:2021441/93‑1 (MQ=255)

                                                                                   tCACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCt        <  2:1236650/93‑1 (MQ=255)

                                                                                    cACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCTg       >  1:3566451/1‑93 (MQ=255)

                                                                                    cACTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCTg       <  2:1566050/93‑1 (MQ=255)

                                                                                     ccTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCTgg      <  1:3347311/92‑1 (MQ=255)

                                                                                      cTCCCTGTCTGCTAAAAATGTGACTCAAAACCCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCTGGt     <  1:2694147/93‑1 (MQ=255)

                                                                                      cTCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCTGGt     <  2:1604102/93‑1 (MQ=255)

                                                                                       tCCCTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCTGGtt    >  1:4405559/1‑93 (MQ=255)

                                                                                         cTTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCTGGTtat  <  1:4019733/93‑1 (MQ=255)

CGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTGCTAAAAATGTGACTCAAAAACCCTTTGCCGGATGGCGGCCCAGCATCTGTTTACATCATTACGACGTCAAACTGCTCCTGGTTAT  >  NC_000913/3393816‑3393997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`