BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,403,945	T→C	V131A (GTA→GCA)	accC →	acetyl CoA carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,403,945	0	T	C	98.9%	587.6 / ‑3.1	188	V131A (GTA→GCA)	accC	acetyl CoA carboxylase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (96/90); total (97/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 199 total aligned reads displayed.

ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403854‑3404034

aTCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAATTATTCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTa                                                                                          >  2:4362465/1‑93 (MQ=255)

  cTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAcc                                                                                        >  1:2263482/1‑93 (MQ=255)

  cTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAcc                                                                                        <  1:2631273/93‑1 (MQ=255)

  cTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCCCGGCGATGAAAAAAGCGGGAGTCCCTTGCGCAcc                                                                                        >  2:1615936/1‑93 (MQ=255)

   ttCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCg                                                                                       <  2:3871621/93‑1 (MQ=255)

   ttCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCg                                                                                       >  1:2782633/1‑93 (MQ=255)

      aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    >  2:3411954/1‑93 (MQ=255)

      aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    >  1:1649828/1‑93 (MQ=255)

      aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    >  2:2229475/1‑93 (MQ=255)

         ggCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCt                                                                                 <  2:2712758/93‑1 (MQ=255)

           cccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGa                                                                               <  2:120375/93‑1 (MQ=255)

            ccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAc                                                                              <  1:3303929/93‑1 (MQ=255)

            ccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAc                                                                              >  1:3092607/1‑93 (MQ=255)

             cGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACg                                                                             >  2:1504127/1‑93 (MQ=255)

              gAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACgg                                                                            >  1:2965959/1‑93 (MQ=255)

               aaaGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGc                                                                           >  2:2912205/1‑93 (MQ=255)

                 aGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGccc                                                                         >  2:4543505/1‑93 (MQ=255)

                  gCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCg                                                                        >  1:2458667/1‑93 (MQ=255)

                  gCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCg                                                                        >  1:3410227/1‑93 (MQ=255)

                   cAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGc                                                                       <  1:3699935/93‑1 (MQ=255)

                    aGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCt                                                                      <  2:2522911/93‑1 (MQ=255)

                    aGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCt                                                                      >  1:3154648/1‑93 (MQ=255)

                     gAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTg                                                                     >  2:4481249/1‑93 (MQ=255)

                      aaaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTgg                                                                    >  1:2014363/1‑93 (MQ=255)

                       aaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTggg                                                                   >  1:1679666/1‑93 (MQ=255)

                        aCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGc                                                                  >  1:402897/1‑93 (MQ=255)

                         ccATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcg                                                                 >  2:4606590/1‑93 (MQ=255)

                          cATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcga                                                                >  2:4038460/1‑93 (MQ=255)

                           aTTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgac                                                               >  1:4676722/1‑93 (MQ=255)

                           aTTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgac                                                               <  2:1558124/93‑1 (MQ=255)

                             ttgCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgacga                                                             <  1:3032832/91‑1 (MQ=255)

                             tCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgacga                                                             <  1:2435000/93‑1 (MQ=255)

                              cGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGat                                                            >  1:1987948/1‑93 (MQ=255)

                               gCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGata                                                           >  2:2336931/1‑93 (MQ=255)

                               gCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGata                                                           <  1:4502980/93‑1 (MQ=255)

                               gCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGata                                                           <  2:2416221/93‑1 (MQ=255)

                                 cTGATTGGCGACAAATTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         <  1:322121/93‑1 (MQ=255)

                                 cTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         <  1:3617640/93‑1 (MQ=255)

                                 cTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         <  2:2324135/93‑1 (MQ=255)

                                  tGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATgg                                                        <  2:2965638/93‑1 (MQ=255)

                                   gATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGa                                                       <  1:2911976/93‑1 (MQ=255)

                                    aTGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAt                                                      <  1:4430750/93‑1 (MQ=255)

                                    aTGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAt                                                      >  2:3696207/1‑93 (MQ=255)

                                    aTGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAt                                                      >  1:2669958/1‑93 (MQ=255)

                                     tGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATa                                                     <  1:4746119/93‑1 (MQ=255)

                                     tGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATa                                                     <  2:4728029/93‑1 (MQ=255)

                                       ggCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaa                                                   <  2:4088851/93‑1 (MQ=255)

                                         cGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaaaa                                                 <  2:3764071/93‑1 (MQ=255)

                                          gACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAc                                                >  1:3897865/1‑93 (MQ=255)

                                           aCAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAcc                                               >  2:3038154/1‑93 (MQ=255)

                                            cAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCg                                              <  2:1210399/93‑1 (MQ=255)

                                             aaaGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGt                                             <  2:202207/93‑1 (MQ=255)

                                                  aTCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAt                                        <  1:3460083/93‑1 (MQ=255)

                                                   tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       <  1:116277/93‑1 (MQ=255)

                                                   tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       <  1:3827665/93‑1 (MQ=255)

                                                   tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       <  1:2241289/93‑1 (MQ=255)

                                                   tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       >  1:484466/1‑93 (MQ=255)

                                                    ccGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTg                                      >  2:395312/1‑93 (MQ=255)

                                                     cGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGc                                     <  1:2265748/93‑1 (MQ=255)

                                                       cAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTa                                   >  1:3633940/1‑93 (MQ=255)

                                                         aTCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTaaa                                 >  2:2620062/1‑93 (MQ=255)

                                                         aTCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTCGGCGACGATATGGATAAAAACCGTGCCATTGCTaaa                                 >  1:477278/1‑93 (MQ=255)

                                                            gcggcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGc                              >  2:704054/1‑93 (MQ=255)

                                                              ggcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAt                            <  2:3240867/93‑1 (MQ=255)

                                                              ggcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAt                            <  2:2080079/93‑1 (MQ=255)

                                                               gcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCGTTGCTAAACGCAtt                           >  2:2178270/1‑93 (MQ=255)

                                                                cgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTg                          >  1:671974/1‑93 (MQ=255)

                                                                 gATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTgg                         <  2:3571819/93‑1 (MQ=255)

                                                                 gATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTgg                         <  2:3084429/93‑1 (MQ=255)

                                                                  aTGAAAAAAGCGGGCGTCCCTTGCTCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGTTATAAACCGTGCCATTGCTAAACTCATTGGt                        >  2:4196345/1‑93 (MQ=255)

                                                                  aTGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGt                        >  2:3593812/1‑93 (MQ=255)

                                                                  aTGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGt                        >  2:4549942/1‑93 (MQ=255)

                                                                   tGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGtt                       >  2:491762/1‑93 (MQ=255)

                                                                   tGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGtt                       >  1:353140/1‑93 (MQ=255)

                                                                   tGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGtt                       >  1:875232/1‑93 (MQ=255)

                                                                   tGAAAAAAGCGGGCGTCCCTGGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGtt                       <  1:3528413/93‑1 (MQ=255)

                                                                     aaaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATAGATAAAAACCGAGCCAGTGCTAAACGCATTGGGTAt                     >  2:886240/1‑93 (MQ=255)

                                                                      aaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATc                    <  1:1234635/93‑1 (MQ=255)

                                                                      aaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCCCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATc                    >  2:2472495/1‑93 (MQ=255)

                                                                       aaaaGCGGGCGTCCCTTGCGCGCCGGGTTCTGACCGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATcc                   <  1:1333075/93‑1 (MQ=255)

                                                                        aaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCg                  >  1:912881/1‑93 (MQ=255)

                                                                         aaGCGCGCCTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCgg                 <  1:3882564/93‑1 (MQ=255)

                                                                             gggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAt             <  2:1923087/93‑1 (MQ=255)

                                                                              ggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAtt            >  2:800530/1‑93 (MQ=255)

                                                                              ggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAtt            >  1:1495634/1‑93 (MQ=255)

                                                                                cGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAt          <  1:4151905/93‑1 (MQ=255)

                                                                                cGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAt          <  1:1264824/93‑1 (MQ=255)

                                                                                cGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAt          <  2:107248/93‑1 (MQ=255)

                                                                                  tCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCa        >  1:386311/1‑93 (MQ=255)

                                                                                    ccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaaa      >  2:656260/1‑93 (MQ=255)

                                                                                    ccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaaa      <  2:3351798/93‑1 (MQ=255)

                                                                                    ccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaaa      <  2:277781/93‑1 (MQ=255)

                                                                                     ctggcgcACCGTGTGCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     <  1:1137385/90‑1 (MQ=255)

                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     >  2:1679587/1‑93 (MQ=255)

                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     <  2:4523288/93‑1 (MQ=255)

                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     >  2:3933092/1‑93 (MQ=255)

                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     >  1:4717779/1‑93 (MQ=255)

                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     >  1:968193/1‑93 (MQ=255)

                                                                                      ttGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGc    <  2:2844040/93‑1 (MQ=255)

                                                                                       tGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGcc   <  2:3465332/93‑1 (MQ=255)

ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403854‑3404034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`