BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,427,261	T→C	V130A (GTC→GCC)	b3279 →	putative transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,427,261	0	T	C	98.1%	493.0 / ‑1.4	157	V130A (GTC→GCC)	b3279	putative transferase
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (61/92); total (62/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 167 total aligned reads displayed.

TTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTG  >  NC_000913/3427171‑3427350

ttGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGccc                                                                                         >  2:355412/1‑93 (MQ=255)

 tGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGcccc                                                                                        <  1:314575/93‑1 (MQ=255)

   gCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCcac                                                                                      >  1:808046/1‑93 (MQ=255)

   gCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCcac                                                                                      <  1:2960459/93‑1 (MQ=255)

      aTCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaa                                                                                   >  2:743748/1‑93 (MQ=255)

      aTCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaa                                                                                   >  2:2477124/1‑93 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              >  1:645086/1‑93 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              <  2:3865446/93‑1 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              <  2:2146966/93‑1 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              <  1:1566493/93‑1 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              >  1:1714343/1‑93 (MQ=255)

            ttttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAAc                                                                             <  1:203508/93‑1 (MQ=255)

             tttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACg                                                                            <  2:1022379/93‑1 (MQ=255)

              ttggttggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACgg                                                                           <  2:3319807/93‑1 (MQ=255)

                ggttggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCt                                                                         <  2:82461/93‑1 (MQ=255)

                 gttggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTg                                                                        <  2:3100762/93‑1 (MQ=255)

                  gtggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTgg                                                                       <  1:3536264/92‑1 (MQ=255)

                   tggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGga                                                                      <  2:1461335/93‑1 (MQ=255)

                   tggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGga                                                                      <  2:1515376/93‑1 (MQ=255)

                   tggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGga                                                                      <  2:1770273/93‑1 (MQ=255)

                    gggATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGgag                                                                     <  2:3276843/93‑1 (MQ=255)

                    gggATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGgag                                                                     <  1:4105214/93‑1 (MQ=255)

                    gggATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGgag                                                                     >  2:1828293/1‑93 (MQ=255)

                       aTGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGagagg                                                                  >  2:3215144/1‑92 (MQ=255)

                       aTGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGc                                                                  <  1:991241/93‑1 (MQ=255)

                        tGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCg                                                                 <  2:3828448/93‑1 (MQ=255)

                        tGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCg                                                                 >  1:1519834/1‑93 (MQ=255)

                          ggCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGa                                                               <  2:1779788/93‑1 (MQ=255)

                           gCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGat                                                              >  1:3067774/1‑93 (MQ=255)

                            cTCAATTTTACTTTATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGata                                                             <  2:2327312/93‑1 (MQ=255)

                            cTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGata                                                             <  1:1047474/93‑1 (MQ=255)

                            cTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGata                                                             >  2:3181727/1‑93 (MQ=255)

                             tCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGatat                                                            >  2:1912208/1‑93 (MQ=255)

                              cAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATc                                                           <  1:2866636/93‑1 (MQ=255)

                              cAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATc                                                           <  1:1284165/93‑1 (MQ=255)

                                 ttttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGt                                                        <  1:4304139/93‑1 (MQ=255)

                                 ttttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGt                                                        >  1:922272/1‑93 (MQ=255)

                                  tttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTa                                                       <  2:1803336/93‑1 (MQ=255)

                                  tttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTa                                                       <  2:765506/93‑1 (MQ=255)

                                   ttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAt                                                      >  1:2232656/1‑93 (MQ=255)

                                    tACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtc                                                     <  1:1087276/93‑1 (MQ=255)

                                    tACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtc                                                     <  1:1737653/93‑1 (MQ=255)

                                    tACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtc                                                     <  1:1423665/93‑1 (MQ=255)

                                     aCTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtct                                                    <  2:1847671/93‑1 (MQ=255)

                                      cTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtctc                                                   <  2:3252685/93‑1 (MQ=255)

                                      attGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtctc                                                   <  1:3173767/92‑1 (MQ=255)

                                        tGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCgg                                                 <  2:301789/93‑1 (MQ=255)

                                        tGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCgg                                                 >  1:4221385/1‑93 (MQ=255)

                                         gATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGt                                                >  2:1088842/1‑93 (MQ=255)

                                          aTGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTa                                               <  2:3446526/93‑1 (MQ=255)

                                           tGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAg                                              <  2:305604/93‑1 (MQ=255)

                                              cgcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGccc                                           <  2:2887532/93‑1 (MQ=255)

                                              cgcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGccc                                           <  2:3595630/93‑1 (MQ=255)

                                               gcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGcccc                                          <  2:358729/93‑1 (MQ=255)

                                               gcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGcccc                                          >  1:3042319/1‑93 (MQ=255)

                                                 aaTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCgc                                        >  1:3321107/1‑92 (MQ=255)

                                                 aaTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGt                                        >  2:1397215/1‑93 (MQ=255)

                                                 aaTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGt                                        <  1:3247519/93‑1 (MQ=255)

                                                 aaTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGt                                        >  1:4396227/1‑93 (MQ=255)

                                                 aaTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCGGGCCCCACAAAATAAACGGCTGGAGATGGGCTATCTGTATCTCGGTAGCCCCGt                                        >  1:4030425/1‑93 (MQ=255)

                                                  aTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTc                                       <  1:3961485/93‑1 (MQ=255)

                                                  aTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTc                                       <  2:4369756/93‑1 (MQ=255)

                                                   tagtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCa                                      <  2:2599910/93‑1 (MQ=255)

                                                   tagtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCa                                      >  1:776572/1‑93 (MQ=255)

                                                    agtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCaa                                     <  2:1405850/93‑1 (MQ=255)

                                                     gtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCaaa                                    >  2:2514699/1‑93 (MQ=255)

                                                     gtagAAGATGATGTGATGATTGGTGCGGATAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGCAGCCCCGTCaaa                                    >  2:2514698/1‑93 (MQ=255)

                                                          agaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGAt                               <  2:501737/93‑1 (MQ=255)

                                                            atgatgTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATcc                             >  2:2325346/1‑93 (MQ=255)

                                                            aggatgtgaggATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATcc                             <  2:1834990/91‑1 (MQ=255)

                                                              gatgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGc                           >  2:170660/1‑93 (MQ=255)

                                                              gatgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGc                           <  1:4047438/93‑1 (MQ=255)

                                                                tgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGccc                         >  2:329852/1‑93 (MQ=255)

                                                                tgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGccc                         >  2:3223056/1‑93 (MQ=255)

                                                                tgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGccc                         >  1:2988576/1‑93 (MQ=255)

                                                                    atgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTa                     >  1:4630224/1‑93 (MQ=255)

                                                                    atgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTa                     <  1:2570680/93‑1 (MQ=255)

                                                                      gatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAg                   <  1:595388/93‑1 (MQ=255)

                                                                      gatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAg                   <  1:2634987/93‑1 (MQ=255)

                                                                      gatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAg                   <  1:1267316/93‑1 (MQ=255)

                                                                       atTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGt                  <  2:206131/93‑1 (MQ=255)

                                                                        ttGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtg                 >  1:299554/1‑93 (MQ=255)

                                                                         tGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtga                <  1:4055351/93‑1 (MQ=255)

                                                                            tGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgatga             >  1:709620/1‑93 (MQ=255)

                                                                             gCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGaa            <  1:126176/93‑1 (MQ=255)

                                                                             gCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGaa            >  1:4637701/1‑93 (MQ=255)

                                                                              ggggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAag           <  2:4047120/92‑1 (MQ=255)

                                                                               gggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAaga          <  1:3639363/93‑1 (MQ=255)

                                                                               gggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAaga          <  1:800842/93‑1 (MQ=255)

                                                                                 gTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAgaga        <  2:4185620/93‑1 (MQ=255)

                                                                                 gTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAgaga        >  1:4447533/1‑93 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       >  2:3452758/1‑93 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       <  1:3068841/93‑1 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       <  1:3855348/93‑1 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAAAAACCGGCTAGAGAGCAGATATCTGTATCTAGGTAGCCCCGTCAAACAGAACCGCCCATTAAGTGATGAAGAGaa       <  2:4352850/93‑1 (MQ=255)

                                                                                   aGTCGGGCCCCACAAAATAAACGGCTGGAGACCGGATATCTGTATCGCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAg      <  1:117117/93‑1 (MQ=255)

                                                                                    gTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAgg     <  2:2939808/93‑1 (MQ=255)

                                                                                       tGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTg  >  2:2062857/1‑93 (MQ=255)

                                                                                       tGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTg  <  2:688595/93‑1 (MQ=255)

                                                                                       tGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGATg  >  1:2377793/1‑93 (MQ=255)

TTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTG  >  NC_000913/3427171‑3427350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`