BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,454,538	T→C	N175N (AAT→AAC)	b3325 →	YheF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,454,538	0	T	C	98.6%	697.2 / ‑3.5	214	N175N (AAT→AAC)	b3325	YheF
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (99/112); total (101/113)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 219 total aligned reads displayed.

CAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCT  >  NC_000913/3454449‑3454627

cAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACaaa                                                                                        >  1:3721037/1‑93 (MQ=255)

cAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACaaa                                                                                        <  2:647529/93‑1 (MQ=255)

cAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACaaa                                                                                        <  2:3648396/93‑1 (MQ=255)

cAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACaaa                                                                                        <  2:2618264/93‑1 (MQ=255)

 aGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAAc                                                                                       >  1:3989288/1‑93 (MQ=255)

  gatgatGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACt                                                                                      >  2:760087/1‑93 (MQ=255)

    tgatgGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGa                                                                                    <  1:3240017/93‑1 (MQ=255)

     gatggatgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGAt                                                                                   <  2:229974/93‑1 (MQ=255)

     gatggatgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGAt                                                                                   <  2:3084293/93‑1 (MQ=255)

      atggatgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGAtt                                                                                  <  2:3930184/93‑1 (MQ=255)

      atggatgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGAtt                                                                                  >  2:2339052/1‑93 (MQ=255)

        ggatgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGa                                                                                <  2:1973979/93‑1 (MQ=255)

        ggatgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGa                                                                                <  1:2930607/93‑1 (MQ=255)

         gatgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGaa                                                                               >  2:2792182/1‑93 (MQ=255)

           tgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGt                                                                             <  1:3073001/93‑1 (MQ=255)

            gCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTc                                                                            >  1:1259010/1‑93 (MQ=255)

              gggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCAt                                                                          >  1:4492766/1‑93 (MQ=255)

              gggTAGCGTCGGTAATGTTGTGCATTATGAACCCTACAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCAt                                                                          <  2:2767937/93‑1 (MQ=255)

                 tAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaaa                                                                       <  2:2141646/93‑1 (MQ=255)

                  aGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAg                                                                      >  2:4032913/1‑93 (MQ=255)

                   gCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgc                                                                     >  2:985514/1‑93 (MQ=255)

                    cGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgcg                                                                    >  2:2426188/1‑93 (MQ=255)

                        ggTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGtt                                                                >  2:1363913/1‑93 (MQ=255)

                        ggTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGtt                                                                >  2:1064444/1‑93 (MQ=255)

                        ggTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGtt                                                                >  2:2233925/1‑93 (MQ=255)

                        ggTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGtt                                                                >  1:10329/1‑93 (MQ=255)

                        ggTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGtt                                                                <  1:1655430/93‑1 (MQ=255)

                         gTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTg                                                               >  1:3303722/1‑93 (MQ=255)

                         gTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTg                                                               >  1:1689002/1‑93 (MQ=255)

                           aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             <  1:1791469/93‑1 (MQ=255)

                           aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             >  1:990716/1‑93 (MQ=255)

                           aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             <  1:469735/93‑1 (MQ=255)

                            aTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATg                                                            >  2:1448305/1‑93 (MQ=255)

                            aTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATg                                                            <  1:328039/93‑1 (MQ=255)

                            aTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTACACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATg                                                            <  1:2690120/93‑1 (MQ=255)

                             tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           >  1:4379657/1‑93 (MQ=255)

                               ttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCa                                                         >  1:962491/1‑93 (MQ=255)

                               ttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATATCa                                                         >  2:1870107/1‑93 (MQ=255)

                                tgtgCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCAt                                                        >  2:4444537/1‑93 (MQ=255)

                                 gtgCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATc                                                       <  1:2809402/93‑1 (MQ=255)

                                   gCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCgg                                                     >  2:3503801/1‑93 (MQ=255)

                                   gCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGGTGATGTCATCgg                                                     >  1:2616211/1‑93 (MQ=255)

                                    cATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGc                                                    <  1:4359054/93‑1 (MQ=255)

                                      ttATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGcac                                                  >  2:2704261/1‑93 (MQ=255)

                                      ttATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGcac                                                  >  2:1796402/1‑93 (MQ=255)

                                        aTGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACag                                                <  2:1668322/93‑1 (MQ=255)

                                         tGACCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACaga                                               <  2:599813/93‑1 (MQ=255)

                                         tGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACaga                                               <  1:1960738/93‑1 (MQ=255)

                                          gAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACagag                                              >  1:1064005/1‑93 (MQ=255)

                                           aaCCCTCCAACGTTCTTATTCTGGCCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAgaga                                             <  2:3486082/93‑1 (MQ=255)

                                           aaCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAgaga                                             <  1:2472227/93‑1 (MQ=255)

                                           aaCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAgaga                                             >  2:950366/1‑93 (MQ=255)

                                           aaCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAgaga                                             <  1:1089107/93‑1 (MQ=255)

                                            aCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGaa                                            >  1:2644404/1‑93 (MQ=255)

                                            aCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGaa                                            <  1:476935/93‑1 (MQ=255)

                                              ccTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAaga                                          >  1:1106777/1‑92 (MQ=255)

                                               cTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAagca                                         >  2:1244254/1‑93 (MQ=255)

                                               cTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAagca                                         <  1:499541/93‑1 (MQ=255)

                                                tCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGGTTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAagcag                                        >  1:2495708/1‑93 (MQ=255)

                                                tCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAagcag                                        >  2:527554/1‑93 (MQ=255)

                                                 ccAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAagcagc                                       <  2:3581732/93‑1 (MQ=255)

                                                 ccAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAagcagc                                       <  2:4393080/93‑1 (MQ=255)

                                                 ccAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAagcagc                                       <  1:4231546/93‑1 (MQ=255)

                                                 ccAACGTTATTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAagcagc                                       <  1:1595851/93‑1 (MQ=255)

                                                  cAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAgcagca                                      >  2:4734135/1‑93 (MQ=255)

                                                  cAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAgcagca                                      >  1:1249279/1‑93 (MQ=255)

                                                    aCGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCaaa                                    >  1:343286/1‑93 (MQ=255)

                                                       ttCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatta                                 <  1:4049227/93‑1 (MQ=255)

                                                       ttCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatta                                 <  2:3296577/93‑1 (MQ=255)

                                                          ttATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTc                              >  1:3343228/1‑93 (MQ=255)

                                                           tATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCa                             <  1:609594/93‑1 (MQ=255)

                                                           tATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCa                             <  1:2301788/93‑1 (MQ=255)

                                                            aTTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCAt                            >  2:3716876/1‑93 (MQ=255)

                                                            aTTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCAt                            >  1:2087101/1‑93 (MQ=255)

                                                             ttCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATc                           <  2:537918/93‑1 (MQ=255)

                                                              tCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCt                          <  1:2427151/93‑1 (MQ=255)

                                                               cTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTg                         <  1:1884147/93‑1 (MQ=255)

                                                               cTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTg                         <  2:3756450/93‑1 (MQ=255)

                                                                tGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTgg                        >  1:2521898/1‑93 (MQ=255)

                                                                  aCCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGaa                      <  1:846271/93‑1 (MQ=255)

                                                                    cGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGTAata                    <  2:2467749/93‑1 (MQ=255)

                                                                    cGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAata                    >  1:3647147/1‑93 (MQ=255)

                                                                    cGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAata                    <  2:1290533/93‑1 (MQ=255)

                                                                        cGTGCCTCCACCATTAACAAACTGATTGAAGTCCTAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCg                <  1:4575497/93‑1 (MQ=255)

                                                                        cGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCg                <  2:2198544/93‑1 (MQ=255)

                                                                        cGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCg                <  2:2001623/93‑1 (MQ=255)

                                                                         gTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGcat               >  1:3681285/1‑91 (MQ=255)

                                                                           gCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCa             <  2:4520152/93‑1 (MQ=255)

                                                                            ccTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATC‑GCCCACAGACACCGCAAATTATTTATCTGGAATATGCGTCAgg           >  1:2527538/1‑92 (MQ=255)

                                                                             cTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGc           <  2:1710786/93‑1 (MQ=255)

                                                                             cTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGc           <  2:2666003/93‑1 (MQ=255)

                                                                              tCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCg          >  1:3645406/1‑93 (MQ=255)

                                                                              tCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCg          >  1:1679503/1‑93 (MQ=255)

                                                                                caccaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGa        >  2:905702/1‑93 (MQ=255)

                                                                                 accaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGaa       <  2:1763430/93‑1 (MQ=255)

                                                                                   caTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGa     <  2:4297402/93‑1 (MQ=255)

                                                                                   caTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGa     <  1:3508019/93‑1 (MQ=255)

                                                                                   caTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGa     <  1:3910025/93‑1 (MQ=255)

                                                                                     ttAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGTAATATGCGTCAGCGGAAGAtc   <  2:4672686/93‑1 (MQ=255)

                                                                                     ttAACAAACTGATTGAAGTCATAAAGAGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAta   >  1:1447074/1‑92 (MQ=255)

CAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCT  >  NC_000913/3454449‑3454627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`