Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 435,927 A→G T39A (ACG→GCG)  pgpA → phosphatidylglycerophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913435,9270AG97.6% 378.4 / ‑2.0 123T39A (ACG→GCG) pgpAphosphatidylglycerophosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (45/75);  total (45/78)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 127 total aligned reads displayed.

GATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATC  >  NC_000913/435837‑436016
                                                                                          |                                                                                         
gATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCg                                                                                         >  2:1400815/1‑93 (MQ=255)
gATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCg                                                                                         >  1:459173/1‑93 (MQ=255)
gATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCg                                                                                         >  2:2278943/1‑93 (MQ=255)
 aTGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGa                                                                                        >  1:4214211/1‑93 (MQ=255)
  tGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAt                                                                                       <  1:1575543/93‑1 (MQ=255)
   gTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATg                                                                                      <  1:2227325/93‑1 (MQ=255)
      gcgAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGc                                                                                   >  2:2225018/1‑93 (MQ=255)
       cgAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCt                                                                                  <  1:1368151/93‑1 (MQ=255)
       cgAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCt                                                                                  <  1:4113411/93‑1 (MQ=255)
        gAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTc                                                                                 <  2:1853775/93‑1 (MQ=255)
         aaaaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCg                                                                                >  2:4480000/1‑93 (MQ=255)
          aaaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGc                                                                               >  2:2705323/1‑93 (MQ=255)
          aaaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGc                                                                               <  1:3891734/93‑1 (MQ=255)
           aaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCt                                                                              <  2:802364/93‑1 (MQ=255)
           aaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCt                                                                              <  1:3163954/93‑1 (MQ=255)
           aaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCt                                                                              <  2:4349205/93‑1 (MQ=255)
            aGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTg                                                                             <  1:2503617/93‑1 (MQ=255)
              tCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGc                                                                           >  1:840844/1‑93 (MQ=255)
               cGCCTGAAGATGTGTCATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGAAGATGGGCTCGCTGGCa                                                                          <  2:4214186/93‑1 (MQ=255)
               cGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCa                                                                          <  2:3342029/93‑1 (MQ=255)
                gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTgcgag                                                                         >  2:3526566/1‑89 (MQ=255)
                gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAg                                                                         >  2:3526565/1‑93 (MQ=255)
                gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAg                                                                         >  1:2251385/1‑93 (MQ=255)
                gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAg                                                                         >  1:2251384/1‑93 (MQ=255)
                 ccTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGc                                                                        <  1:3734077/93‑1 (MQ=255)
                 ccTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGc                                                                        <  1:1164483/93‑1 (MQ=255)
                  cTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCg                                                                       >  1:4234695/1‑93 (MQ=255)
                   tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGa                                                                      >  1:2083690/1‑93 (MQ=255)
                   tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGa                                                                      <  1:100211/93‑1 (MQ=255)
                    gAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGAt                                                                     <  1:283233/93‑1 (MQ=255)
                     aaGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGAtt                                                                    <  2:1603116/93‑1 (MQ=255)
                      aGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTc                                                                   <  1:3447368/93‑1 (MQ=255)
                       gATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTcc                                                                  <  2:1842505/93‑1 (MQ=255)
                       gATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTcc                                                                  <  2:911674/93‑1 (MQ=255)
                       gATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTcc                                                                  <  2:3897567/93‑1 (MQ=255)
                       gATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGATGGGCTCGCTGGCAGCGATTcc                                                                  <  2:3284611/93‑1 (MQ=255)
                          gAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGtt                                                               >  2:2634050/1‑93 (MQ=255)
                           aGTAATCCGTGTCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTc                                                              <  1:3514356/93‑1 (MQ=255)
                           aGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTc                                                              <  1:946211/93‑1 (MQ=255)
                            gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCt                                                             <  1:3287940/93‑1 (MQ=255)
                            gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCt                                                             <  1:2314529/93‑1 (MQ=255)
                            gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCt                                                             >  2:4104289/1‑93 (MQ=255)
                              aaTCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTgg                                                           <  1:3757680/93‑1 (MQ=255)
                               aTCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGt                                                          <  1:4351042/93‑1 (MQ=255)
                                tCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGCTCTGGTa                                                         >  1:1914508/1‑93 (MQ=255)
                                      gCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGCTCTGGTATCtgat                                                   <  1:3134801/93‑1 (MQ=255)
                                       cATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCtgatg                                                  <  2:3687131/93‑1 (MQ=255)
                                       cATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCACCGATTCCGTTCTGGTATCtgatg                                                  >  1:380012/1‑93 (MQ=255)
                                         tCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGAc                                                <  2:4198739/93‑1 (MQ=255)
                                           tACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCt                                              <  1:3115731/93‑1 (MQ=255)
                                           tACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCt                                              <  1:4500304/93‑1 (MQ=255)
                                             cTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                            <  2:2758835/93‑1 (MQ=255)
                                             cTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                            <  2:4660046/93‑1 (MQ=255)
                                             cTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                            <  2:1524036/93‑1 (MQ=255)
                                              ttGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGTTTCCGTTCTGGTATCTGATGACCtttt                                           >  2:1148122/1‑93 (MQ=255)
                                              ttGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtttt                                           <  1:1149243/93‑1 (MQ=255)
                                              ttGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtttt                                           >  2:1148121/1‑93 (MQ=255)
                                              ttGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtttt                                           <  2:4301348/93‑1 (MQ=255)
                                              ttGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtttt                                           <  2:876065/93‑1 (MQ=255)
                                              ttGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtttt                                           >  2:743902/1‑93 (MQ=255)
                                               tGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttttt                                          <  2:1644638/93‑1 (MQ=255)
                                               tGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttttt                                          <  2:4627449/93‑1 (MQ=255)
                                                gCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTg                                         <  1:2753998/93‑1 (MQ=255)
                                                 cTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGc                                        <  2:2351222/93‑1 (MQ=255)
                                                 cTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGc                                        >  2:4687416/1‑93 (MQ=255)
                                                  tGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGcc                                       >  1:2101447/1‑93 (MQ=255)
                                                   gTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGcgc                                      >  2:838461/1‑91 (MQ=255)
                                                   gTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGGATCTGATGACCTTTTTGccc                                      >  2:1907233/1‑93 (MQ=255)
                                                    tCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCt                                     <  2:1165104/93‑1 (MQ=255)
                                                    tCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCt                                     <  2:3196437/93‑1 (MQ=255)
                                                     gggATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTg                                    >  2:2881165/2‑93 (MQ=255)
                                                      ggATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTgg                                   >  1:17682/1‑93 (MQ=255)
                                                      ggATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTgg                                   >  1:3279132/1‑93 (MQ=255)
                                                           cGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGct                              <  2:2209574/93‑1 (MQ=255)
                                                           cGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGct                              >  2:2857016/1‑93 (MQ=255)
                                                            ggAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGctc                             <  1:972749/93‑1 (MQ=255)
                                                             gAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGctct                            >  1:2916243/1‑93 (MQ=255)
                                                              aaGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTa                           >  2:1501548/1‑93 (MQ=255)
                                                               aGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTAc                          >  2:1311826/1‑93 (MQ=255)
                                                                    gttAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCt                     <  1:2253851/92‑1 (MQ=255)
                                                                    aTTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCt                     >  2:797559/1‑93 (MQ=255)
                                                                     ttAAGACCGATAGTTCCTGGGGCGATAGGCTCGCTGGCAGCAATTCCGTTCTGGTATCTGATGACCTTTTTACCCTGGCAGCACTACTCGCtg                    <  2:4200060/93‑1 (MQ=255)
                                                                       aggCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggt                  <  1:92601/91‑1 (MQ=255)
                                                                       aaGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggt                  <  1:3642940/93‑1 (MQ=255)
                                                                       aaGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggt                  <  2:2883650/93‑1 (MQ=255)
                                                                         gCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggtgg                <  1:2613483/93‑1 (MQ=255)
                                                                          cccGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTggtggt               <  1:1529921/93‑1 (MQ=255)
                                                                          cccGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCCCtggtggg               >  1:1582253/1‑92 (MQ=255)
                                                                            cGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGa             <  2:2103417/93‑1 (MQ=255)
                                                                             gATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGAt            >  2:208985/1‑93 (MQ=255)
                                                                              aTCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATg           >  2:4298584/1‑93 (MQ=255)
                                                                              aTCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATg           >  2:2665910/1‑93 (MQ=255)
                                                                                cGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCt         >  1:4590360/1‑93 (MQ=255)
                                                                                   tCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTggg      <  1:2137574/93‑1 (MQ=255)
                                                                                    ccTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTgggg     <  1:1523687/93‑1 (MQ=255)
                                                                                     cTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGa    <  1:1783942/93‑1 (MQ=255)
                                                                                     cTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGa    <  2:2838598/93‑1 (MQ=255)
                                                                                      tGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGAt   <  1:1803340/93‑1 (MQ=255)
                                                                                       ggggCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATc  <  2:439869/93‑1 (MQ=255)
                                                                                       ggggCGAGGGGCTCGCTGGCAGCGTTTCCGTTCTGGCAACTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCGGGCGGGGATGCGGGGGacc  >  1:3617518/1‑91 (MQ=255)
                                                                                          |                                                                                         
GATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATC  >  NC_000913/435837‑436016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑