Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,483,814 C→T H20Y (CAC→TAC)  crp → cyclic AMP receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,483,8140CT90.7% 720.3 / 36.1 237H20Y (CAC→TAC) crpcyclic AMP receptor protein
Reads supporting (aligned to +/- strand):  ref base C (12/10);  new base T (107/108);  total (119/118)
Fisher's exact test for biased strand distribution p-value = 8.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 241 total aligned reads displayed.

TGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTG  >  NC_000913/3483725‑3483906
                                                                                         |                                                                                            
tGGAGAAAGCTTCTACCCGAGGATAACCGCGCATGGTGCTTCGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCtaca                                                                                           <  2:505807/93‑1 (MQ=255)
 ggAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCCTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAt                                                                                          >  2:623388/1‑93 (MQ=255)
  gagaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAtt                                                                                         <  1:2315502/93‑1 (MQ=255)
  gagaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACAtt                                                                                         >  1:3234959/1‑93 (MQ=255)
   agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTc                                                                                        <  2:1860218/93‑1 (MQ=255)
    gaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCa                                                                                       >  1:2407146/1‑93 (MQ=255)
     aaaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCAt                                                                                      <  1:158552/93‑1 (MQ=255)
      aaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCTACCTTCAtc                                                                                     >  1:3949387/1‑92 (MQ=255)
      aaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATa                                                                                     >  2:4547504/1‑93 (MQ=255)
      aaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATa                                                                                     <  2:3445539/93‑1 (MQ=255)
      aaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATa                                                                                     <  2:2917220/93‑1 (MQ=255)
       aGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATaa                                                                                    <  1:4031992/93‑1 (MQ=255)
        gCTTATAGCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                   <  2:683257/93‑1 (MQ=255)
        gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                   <  2:1241989/93‑1 (MQ=255)
        gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                   >  1:544429/1‑93 (MQ=255)
        gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAg                                                                                   >  2:3833075/1‑93 (MQ=255)
          ttATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTa                                                                                 >  1:1176577/1‑93 (MQ=255)
           tataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTAc                                                                                >  2:2393685/1‑93 (MQ=255)
           tataACAGAGGATAACCGCACATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTAc                                                                                >  2:2495468/1‑93 (MQ=255)
              aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCa                                                                             <  2:4363728/93‑1 (MQ=255)
              aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCa                                                                             <  2:4483823/93‑1 (MQ=255)
               aCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCAt                                                                            >  2:4657013/1‑93 (MQ=255)
                 cgcggATAACGGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATcc                                                                          <  1:43990/90‑1 (MQ=255)
                  gagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCa                                                                         >  1:3825385/1‑93 (MQ=255)
                     gATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAaga                                                                      >  2:1442740/1‑93 (MQ=255)
                      aTAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAagag                                                                     >  2:4563842/1‑93 (MQ=255)
                        aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCa                                                                   <  1:1013249/93‑1 (MQ=255)
                        aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCa                                                                   >  1:2973231/1‑93 (MQ=255)
                         aCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCAc                                                                  <  1:2730623/93‑1 (MQ=255)
                           cgcgCCTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGc                                                                <  1:321325/93‑1 (MQ=255)
                           cgcgCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGc                                                                >  1:2223593/1‑93 (MQ=255)
                           cgcgCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGc                                                                <  1:1018274/93‑1 (MQ=255)
                            gcgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCt                                                               >  1:4331587/1‑93 (MQ=255)
                              gcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTa                                                             <  2:4208618/93‑1 (MQ=255)
                              gcATGGTGCTTGGAAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCttt                                                             >  1:3694879/1‑92 (MQ=255)
                               cATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTAt                                                            <  2:4147197/93‑1 (MQ=255)
                                 tGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTc                                                          <  2:3395664/93‑1 (MQ=255)
                                  ggTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCa                                                         >  1:1450290/1‑93 (MQ=255)
                                     gCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCa                                                      >  1:2148273/1‑93 (MQ=255)
                                     gCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGGTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCa                                                      >  1:1050165/1‑93 (MQ=255)
                                      cTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAg                                                     <  2:1035637/93‑1 (MQ=255)
                                       ttGTCAAACCGCAAACAGACCCGACTCTCGAATAGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                    <  1:4587643/93‑1 (MQ=255)
                                       ttGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                    <  2:3619886/93‑1 (MQ=255)
                                       ttGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                    <  2:3242608/93‑1 (MQ=255)
                                        tGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAggg                                                   <  1:4156533/93‑1 (MQ=255)
                                        tGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAggg                                                   <  1:888863/93‑1 (MQ=255)
                                         ggCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGt                                                  <  1:2047412/93‑1 (MQ=255)
                                          gCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTg                                                 <  2:3930445/93‑1 (MQ=255)
                                             aaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                              >  1:3974097/1‑93 (MQ=255)
                                             aaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                              <  1:2354833/93‑1 (MQ=255)
                                             aaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                              >  2:2313984/1‑93 (MQ=255)
                                               ccGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                            <  2:2086805/93‑1 (MQ=255)
                                               ccGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                            <  2:2732829/93‑1 (MQ=255)
                                                cGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAg                                           <  2:2829105/93‑1 (MQ=255)
                                                   aaaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCgg                                        >  2:886004/1‑93 (MQ=255)
                                                    aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGa                                       <  1:2551575/93‑1 (MQ=255)
                                                    aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGa                                       >  1:2807288/1‑93 (MQ=255)
                                                     aCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaa                                      <  1:1331271/93‑1 (MQ=255)
                                                      cggACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCGCGCTTATTCACCAGGGTGAAAAAGCGGaaa                                     <  1:2283368/91‑1 (MQ=255)
                                                      cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaaa                                     <  1:1980769/93‑1 (MQ=255)
                                                       aGACCCGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                    <  1:290350/93‑1 (MQ=255)
                                                       aGACCCGACTCTCGAATGGATCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                    >  1:3703379/1‑93 (MQ=255)
                                                          cccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                 <  2:433216/93‑1 (MQ=255)
                                                          cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGAGAAAAAGCGGCAACGCt                                 >  2:4035139/1‑93 (MQ=255)
                                                           ccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                                >  2:4082860/1‑93 (MQ=255)
                                                           ccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                                <  2:736522/93‑1 (MQ=255)
                                                           ccGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                                <  1:2605998/93‑1 (MQ=255)
                                                            cGACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                               >  2:3903706/1‑93 (MQ=255)
                                                             gACTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCATATTCACCAGGGTGAAAAAGCGGAAACGCTGta                              >  2:4391340/1‑93 (MQ=255)
                                                              aCTCTCGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtac                             >  2:3570610/1‑93 (MQ=255)
                                                                 ctcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtactac                          >  1:339525/1‑93 (MQ=255)
                                                                  tcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTCCa                         >  2:1584203/1‑93 (MQ=255)
                                                                  tcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                         <  1:659671/93‑1 (MQ=255)
                                                                  tcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                         <  2:3295798/93‑1 (MQ=255)
                                                                  tcGAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                         >  2:3684583/1‑93 (MQ=255)
                                                                  tcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                         >  1:1873773/1‑93 (MQ=255)
                                                                    gAATGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATc                       >  2:279047/1‑93 (MQ=255)
                                                                      aTGGTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGt                     <  1:2175265/93‑1 (MQ=255)
                                                                        ggTTCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                   <  2:2101890/93‑1 (MQ=255)
                                                                          ttCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa                 <  2:101242/93‑1 (MQ=255)
                                                                           tCTTGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGCAAAAGCGGCAACGCTGTACTACATCGTTAAAg                >  2:2421870/1‑93 (MQ=255)
                                                                             ttGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGc              >  1:3254558/1‑93 (MQ=255)
                                                                             ttGTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGc              <  2:2587283/93‑1 (MQ=255)
                                                                               gTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc            <  1:1226374/93‑1 (MQ=255)
                                                                               gTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc            >  1:3001272/1‑93 (MQ=255)
                                                                               gTCTCATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGCGAAAAAGCGGAACCGCTGTACTACATCGTTAAAGActc            >  2:3713463/1‑93 (MQ=255)
                                                                               gTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc            >  2:3583988/1‑93 (MQ=255)
                                                                                tctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct           <  1:1427610/93‑1 (MQ=255)
                                                                                tctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct           >  2:4173876/1‑93 (MQ=255)
                                                                                tctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct           >  2:613318/1‑93 (MQ=255)
                                                                                tctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct           >  1:4107757/1‑93 (MQ=255)
                                                                                 ctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtg          >  1:170473/1‑93 (MQ=255)
                                                                                 ctcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtg          <  2:984431/93‑1 (MQ=255)
                                                                                  tcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt         >  2:2288518/1‑93 (MQ=255)
                                                                                  tcATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt         <  1:721199/93‑1 (MQ=255)
                                                                                   cATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg        <  2:1313562/93‑1 (MQ=255)
                                                                                   cATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg        <  2:1204595/93‑1 (MQ=255)
                                                                                   cATTGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGGGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg        >  1:3560985/1‑93 (MQ=255)
                                                                                     ttGCTACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGc      >  2:3020139/1‑93 (MQ=255)
                                                                                         cacaTTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTg  <  2:3165709/93‑1 (MQ=255)
                                                                                         |                                                                                            
TGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTG  >  NC_000913/3483725‑3483906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑