BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,507,831	T→C	T262A (ACT→GCT)	yhfX ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,831	0	T	C	98.4%	579.3 / ‑2.7	184	T262A (ACT→GCT)	yhfX	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (80/101); total (83/101)
Fisher's exact test for biased strand distribution p-value = 9.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 193 total aligned reads displayed.

AGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGC  >  NC_000913/3507741‑3507916

 gCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGcc                                                                                    >  1:4252185/1‑93 (MQ=255)

 gCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGcc                                                                                    >  1:840014/1‑93 (MQ=255)

 gCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGcc                                                                                    <  1:3066194/93‑1 (MQ=255)

   tGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCg                                                                                  >  1:3634085/1‑93 (MQ=255)

   tGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCg                                                                                  >  2:1213748/1‑93 (MQ=255)

   tGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCg                                                                                  >  2:3442039/1‑93 (MQ=255)

   tGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTCCTGGTTTGCCGGAATAGCGCCCg                                                                                  >  2:2144633/1‑93 (MQ=255)

    gTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCAGGAATAGCGCCCGt                                                                                 >  1:1600938/1‑93 (MQ=255)

       gCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAg                                                                              >  2:2246357/1‑93 (MQ=255)

        ccACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAgc                                                                             <  1:2099485/93‑1 (MQ=255)

          aCGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAgcgc                                                                           >  1:2749840/1‑93 (MQ=255)

           cGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCa                                                                          <  2:2364385/93‑1 (MQ=255)

            ggAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCAt                                                                         >  2:1480173/1‑93 (MQ=255)

             gAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATg                                                                        >  2:4486654/1‑93 (MQ=255)

             gAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATg                                                                        >  2:4416548/1‑93 (MQ=255)

             gAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATg                                                                        <  2:1497349/93‑1 (MQ=255)

              aaaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGa                                                                       <  2:1364946/93‑1 (MQ=255)

               aaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAc                                                                      <  2:3882778/93‑1 (MQ=255)

               aaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAc                                                                      <  1:760329/93‑1 (MQ=255)

               aaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAc                                                                      >  2:429065/1‑93 (MQ=255)

                 tgatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCg                                                                    <  1:2903593/93‑1 (MQ=255)

                  gatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCgg                                                                   <  2:496483/93‑1 (MQ=255)

                    tgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCTTGACCGGGt                                                                 >  2:3710953/1‑93 (MQ=255)

                    tgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGt                                                                 <  1:3339864/93‑1 (MQ=255)

                     gggATATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                <  2:908880/93‑1 (MQ=255)

                     gggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                <  1:1586794/93‑1 (MQ=255)

                      ggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTc                                                               <  2:1836294/93‑1 (MQ=255)

                      ggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTc                                                               >  1:3761933/1‑93 (MQ=255)

                          aTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCa                                                           <  2:2014987/93‑1 (MQ=255)

                          aTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCa                                                           >  1:4705474/1‑93 (MQ=255)

                          aTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCa                                                           <  1:2670652/93‑1 (MQ=255)

                           tttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAt                                                          >  2:4242773/1‑93 (MQ=255)

                           tttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAt                                                          <  1:2546241/93‑1 (MQ=255)

                           tttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAt                                                          >  1:4646632/1‑93 (MQ=255)

                           tttCACTTAACCAGAGCATCGCGATACGTTAAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAt                                                          >  2:1013084/1‑93 (MQ=255)

                            ttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATg                                                         <  1:1211999/93‑1 (MQ=255)

                            ttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATg                                                         >  1:3193235/1‑93 (MQ=255)

                             tCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCTCCCGTCAGCGCATGACCGGGTTCGGCATGa                                                        >  2:1197561/1‑93 (MQ=255)

                             tCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGa                                                        >  1:1096677/1‑93 (MQ=255)

                               aCTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGt                                                      <  1:4612899/93‑1 (MQ=255)

                                cTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTc                                                     >  1:2372443/1‑93 (MQ=255)

                                 ttAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTca                                                    <  2:2880653/93‑1 (MQ=255)

                                  tAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTcac                                                   >  2:1942977/1‑93 (MQ=255)

                                  tAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTcac                                                   <  2:4037548/93‑1 (MQ=255)

                                   aaCCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTcaca                                                  <  1:399211/93‑1 (MQ=255)

                                   aaCCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTcaca                                                  >  1:2404354/1‑93 (MQ=255)

                                    aCCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCacac                                                 <  1:893422/93‑1 (MQ=255)

                                    aCCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCacac                                                 <  2:163096/93‑1 (MQ=255)

                                    aCCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCacac                                                 <  1:1449566/93‑1 (MQ=255)

                                      cAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCg                                               >  1:460227/1‑93 (MQ=255)

                                       agagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGt                                              >  2:32176/1‑93 (MQ=255)

                                        gagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTa                                             <  1:125464/93‑1 (MQ=255)

                                        gagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTa                                             >  1:2994178/1‑93 (MQ=255)

                                         agCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAt                                            >  2:3860492/1‑93 (MQ=255)

                                         agCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAt                                            <  1:3360622/93‑1 (MQ=255)

                                         agCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAt                                            >  1:4345493/1‑93 (MQ=255)

                                          gCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAtt                                           <  1:8254/93‑1 (MQ=255)

                                          gCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAtt                                           <  1:41563/93‑1 (MQ=255)

                                           cATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTg                                          >  1:46416/1‑93 (MQ=255)

                                            aTCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTgc                                         >  2:3295865/1‑93 (MQ=255)

                                             tCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTgcg                                        >  2:3461463/1‑93 (MQ=255)

                                             tCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTgcg                                        >  2:1660943/1‑93 (MQ=255)

                                                cgATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTGGGCAGGGGTCACACCGTATTGCGccg                                     >  1:79895/1‑92 (MQ=255)

                                                cgATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCa                                     <  1:2282093/93‑1 (MQ=255)

                                                cgATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCa                                     >  1:4519250/1‑93 (MQ=255)

                                                 gATACGTTCTGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAg                                    <  2:4076835/93‑1 (MQ=255)

                                                  aTACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGt                                   >  1:954633/1‑93 (MQ=255)

                                                   tACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTa                                  >  1:4139890/1‑93 (MQ=255)

                                                     cGTTCAGGCTGATCGCCCTGCTGGTGTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAAt                                <  1:612186/93‑1 (MQ=255)

                                                      gTTCAGGCTGATCGCACTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATg                               <  2:4562330/93‑1 (MQ=255)

                                                       ttCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATgg                              <  1:3527098/93‑1 (MQ=255)

                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                             >  2:1169321/1‑93 (MQ=255)

                                                         cAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCa                            <  1:3701240/93‑1 (MQ=255)

                                                         cAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCa                            <  1:179166/93‑1 (MQ=255)

                                                          aGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAg                           >  1:3278591/1‑93 (MQ=255)

                                                           ggCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGc                          <  1:4258675/93‑1 (MQ=255)

                                                             cTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGa                        <  2:561567/93‑1 (MQ=255)

                                                              tGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGaa                       >  1:650800/1‑93 (MQ=255)

                                                                aTCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGt                     <  1:3381119/93‑1 (MQ=255)

                                                                 tCGCCCTGCTGGTTTGCCGGAATAGCTCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATGGCGCCAGTAATGGCAGCGAAGTg                    <  2:1102362/93‑1 (MQ=255)

                                                                   gCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCa                  >  2:3777230/1‑93 (MQ=255)

                                                                    cccTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAg                 <  2:820199/93‑1 (MQ=255)

                                                                     ccTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGc                <  1:4441642/93‑1 (MQ=255)

                                                                      ctgctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATTGCTGCGAAGTGCAGCt               >  2:3595068/1‑93 (MQ=255)

                                                                      ctgctgGTTTGACGGAATAGCGCCCGTCAGAGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCt               >  2:4732682/1‑93 (MQ=255)

                                                                       tgctgGTTTGTGTGAATAGCGCCCGTCAGCGGATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTg              <  1:4293415/93‑1 (MQ=255)

                                                                        gctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTgg             <  1:2131654/93‑1 (MQ=255)

                                                                        gctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTgg             <  2:2286934/93‑1 (MQ=255)

                                                                          tgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTc           >  1:2530269/1‑93 (MQ=255)

                                                                            gTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGc         <  2:1479610/93‑1 (MQ=255)

                                                                             tttGCCGGAATAGCGCCCGTCAGCGCATGACCGGGATCGGCATGAGTCACACCGTATTGCGCCAGCAATGGCAGTGAAGGGCAGCTGGTCGCt        >  1:2705700/1‑93 (MQ=255)

                                                                               tGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGa      <  1:4656491/93‑1 (MQ=255)

                                                                                gCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGaa     <  2:2989662/93‑1 (MQ=255)

                                                                                gCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGaa     <  1:133829/93‑1 (MQ=255)

                                                                                gCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGaa     <  2:4623696/93‑1 (MQ=255)

                                                                                 ccGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAg    <  2:2749840/93‑1 (MQ=255)

                                                                                 ccGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAg    <  2:2417300/93‑1 (MQ=255)

                                                                                 ccGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAg    <  1:3580013/93‑1 (MQ=255)

                                                                                  cGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAgg   <  2:465621/93‑1 (MQ=255)

                                                                                   cgAATAGCGCCCGTCAGCGCATGACCGTCTTCGGCATGATCCCCACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGgc  <  1:2325950/92‑1 (MQ=255)

AGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGC  >  NC_000913/3507741‑3507916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`