BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,595,797	A→G	G61G (GGA→GGG)	yhhK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,595,797	0	A	G	99.3%	477.6 / ‑4.7	150	G61G (GGA→GGG)	yhhK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (72/77); total (73/77)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01

Only 100 of 161 total aligned reads displayed.

ACAGGTTGACGATAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGAGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACAATCCTGG  >  NC_000913/3595707‑3595886

 cAGGTTGACGATAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCa                                                                                        >  2:3317730/1‑93 (MQ=255)

  aGGTTGACGATAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCAc                                                                                       >  1:413502/1‑93 (MQ=255)

   ggTTGACGATAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACt                                                                                      >  1:3134354/1‑93 (MQ=255)

    gTTGACGATAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTg                                                                                     >  2:2299721/1‑93 (MQ=255)

     ttGACGATAACCACCGTATCTACGCCGGGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTATCCTTAAGCGGCTCCGAGGGGGCACTga                                                                                    <  2:2866809/93‑2 (MQ=255)

     ttGACGATAACCACCGTATCTACGCCGCGCGCTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTgg                                                                                    >  1:2009956/1‑93 (MQ=255)

      tGACGATAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGa                                                                                   <  1:4008581/93‑1 (MQ=255)

        aCGATAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGAtt                                                                                 >  1:2771004/1‑93 (MQ=255)

          gATAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTcc                                                                               >  2:2774166/1‑93 (MQ=255)

            tAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCt                                                                             <  1:779211/93‑1 (MQ=255)

             aaCCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTTGCTTCCCTg                                                                            >  2:108184/1‑93 (MQ=255)

             aaCCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTg                                                                            >  2:2531028/1‑93 (MQ=255)

              accaccGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTgc                                                                           >  2:2002270/1‑93 (MQ=255)

              accaccGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTgc                                                                           >  2:1971226/1‑93 (MQ=255)

              accaccGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTgc                                                                           >  1:1183717/1‑93 (MQ=255)

                 accGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGcgcg                                                                        >  2:3442252/1‑93 (MQ=255)

                 accGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGcgcg                                                                        >  2:3640529/1‑93 (MQ=255)

                  ccGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGt                                                                       <  2:406687/93‑1 (MQ=255)

                   cGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTg                                                                      <  2:1056532/93‑1 (MQ=255)

                    gTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGc                                                                     <  1:2259488/93‑1 (MQ=255)

                    gTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGc                                                                     >  1:2142666/1‑93 (MQ=255)

                    gTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGc                                                                     >  2:66003/1‑93 (MQ=255)

                    gTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGc                                                                     >  1:1240763/1‑93 (MQ=255)

                       tCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCggg                                                                  >  1:4199257/1‑93 (MQ=255)

                       tCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCggg                                                                  >  1:96957/1‑93 (MQ=255)

                       tCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCggg                                                                  <  2:797417/93‑1 (MQ=255)

                         tACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGaa                                                                <  1:3617322/93‑1 (MQ=255)

                            gccgcGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGta                                                             >  2:4322647/1‑92 (MQ=255)

                            gccgcGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTc                                                             <  2:1519227/93‑1 (MQ=255)

                            gccgcGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTc                                                             <  1:1932316/93‑1 (MQ=255)

                            gccgcGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTc                                                             >  2:592425/1‑93 (MQ=255)

                              cgcgcgTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCAc                                                           <  2:3234843/93‑1 (MQ=255)

                               gcgcgTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCAcc                                                          <  1:2610004/93‑1 (MQ=255)

                               gcgcgTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCAcc                                                          >  2:1376224/1‑93 (MQ=255)

                               gcgcgTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCAcc                                                          <  2:4747936/93‑1 (MQ=255)

                                cgcgTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCAccc                                                         <  1:283770/93‑1 (MQ=255)

                                 gcgTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACccg                                                        >  1:3322854/1‑93 (MQ=255)

                                   gTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACccgcc                                                      >  2:91285/1‑93 (MQ=255)

                                     tttAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGt                                                    <  1:4446694/93‑1 (MQ=255)

                                      ttAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTc                                                   <  1:1012384/93‑1 (MQ=255)

                                      ttAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTc                                                   <  2:663327/93‑1 (MQ=255)

                                       tAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTcg                                                  <  2:163853/93‑1 (MQ=255)

                                        aaCGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTcgc                                                 >  1:1009414/1‑93 (MQ=255)

                                        aaCGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTcgc                                                 >  1:2336089/1‑93 (MQ=255)

                                         aCGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTcgcg                                                >  2:743223/1‑93 (MQ=255)

                                            aGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGTGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGgtg                                             >  2:1037319/1‑93 (MQ=255)

                                             gcgcCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGgtgt                                            >  2:2995775/1‑93 (MQ=255)

                                             gcgcCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGgtgt                                            <  2:3822685/93‑1 (MQ=255)

                                                ccTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTggg                                         <  1:1261672/93‑1 (MQ=255)

                                                 cTGCTCGCTGCCGTGCGGGTAACCTTCAGCGGCACCGAGGGGGCACTGGATTCCCAGCGCGTGCGGAAAGTCACCCGCCGTCGCGGTGTgggg                                        <  2:4212907/93‑1 (MQ=255)

                                                  tGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGc                                       >  2:1547252/1‑93 (MQ=255)

                                                  tGCTCGCTGCCGTGCGGGCAACCTTAAGCGGCACCGAGGGGGCACTGGATTACCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGc                                       >  2:4434783/1‑93 (MQ=255)

                                                  cgCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCCCCCGCCGTCGCGGTGTGGGGc                                       <  2:4686114/92‑1 (MQ=255)

                                                   gCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCa                                      <  1:1391113/93‑1 (MQ=255)

                                                    cTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCaa                                     <  1:728360/93‑1 (MQ=255)

                                                     tCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAat                                    <  1:62417/93‑1 (MQ=255)

                                                      cGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGTCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAata                                   <  1:3049549/93‑1 (MQ=255)

                                                       gCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAatat                                  <  2:1482195/93‑1 (MQ=255)

                                                       gCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGCGTGGGGCAatat                                  >  2:3857567/1‑93 (MQ=255)

                                                       gCTGCCGTGCGGGAAACCTTAAGCGGCACCGAGGGGGAACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGAGTGGGGCAatac                                  >  2:1248902/1‑92 (MQ=255)

                                                        cTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATc                                 >  2:2633096/1‑93 (MQ=255)

                                                        cTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATc                                 <  1:3756418/93‑1 (MQ=255)

                                                        cTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGCCGCGGTGTGGGGCAATATc                                 >  2:3558537/1‑93 (MQ=255)

                                                         tGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATct                                >  2:4088009/1‑93 (MQ=255)

                                                         tGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGCGAAGTCACCCGCCGTCGCGGTGTGGGGCACTATct                                >  2:2142645/1‑93 (MQ=255)

                                                          gCCGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATctg                               >  2:2034494/1‑93 (MQ=255)

                                                           ccGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATctgc                              <  1:4351796/93‑1 (MQ=255)

                                                           ccGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATctgc                              <  1:55266/93‑1 (MQ=255)

                                                           ccGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATctgc                              <  1:2357842/93‑1 (MQ=255)

                                                            cGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATctgct                             >  2:2610220/1‑93 (MQ=255)

                                                            cGTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATctgct                             <  2:3483389/93‑1 (MQ=255)

                                                             gTGCGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATctgctg                            >  1:1402585/1‑93 (MQ=255)

                                                                cGGGTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGaa                         <  1:1543993/93‑1 (MQ=255)

                                                                 gggTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAag                        <  1:173182/93‑1 (MQ=255)

                                                                 gggTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAag                        >  2:3906650/1‑93 (MQ=255)

                                                                 gggTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCAGCCGTCGCGGTGTGGGGCAATATCTGCTGGAag                        <  1:341133/93‑1 (MQ=255)

                                                                  ggTAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAaga                       <  2:3121980/93‑1 (MQ=255)

                                                                   gTAACCTTAAGCGGCACCGAGGGAGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAagag                      >  2:1591796/1‑93 (MQ=255)

                                                                    tAACCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCCCCCGCCGTCGCGGTGTGGGGCAATATCTACTGGAAGAgg                     <  2:4195662/93‑1 (MQ=255)

                                                                     aaCCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGt                    <  1:3764666/93‑1 (MQ=255)

                                                                      aCCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGtt                   >  2:4681619/1‑93 (MQ=255)

                                                                      aCCTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGtt                   <  2:1956409/93‑1 (MQ=255)

                                                                       ccTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGttt                  >  2:1333749/1‑93 (MQ=255)

                                                                        cTTAAGCGGCACCGGGGGGGCACTGGATTCCCTGCCCGTGCGGGAAGTCCACCGACGTCGCGGTGTGGGGCAATATCTCCTGGAAACGGtttg                 >  2:3535307/1‑92 (MQ=255)

                                                                        cTTAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGtttt                 <  1:3166692/93‑1 (MQ=255)

                                                                          tAAGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGc               >  2:3063179/1‑93 (MQ=255)

                                                                           aaGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCg              >  1:1607525/1‑93 (MQ=255)

                                                                            aGCGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGt             <  2:629215/93‑1 (MQ=255)

                                                                             gcgcCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCCCCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTa            <  1:4622652/93‑1 (MQ=255)

                                                                              cGGCACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTaa           <  1:676549/93‑1 (MQ=255)

                                                                                 cACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACaa        <  1:2506946/93‑1 (MQ=255)

                                                                                 cACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACaa        <  2:769159/93‑1 (MQ=255)

                                                                                 cACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACaa        <  2:1921008/93‑1 (MQ=255)

                                                                                 cACCGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACaa        <  1:2506945/93‑1 (MQ=255)

                                                                                   ccGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACAATc      >  1:2134182/1‑93 (MQ=255)

                                                                                    cGAGGGGGCCCTGGATTCCCTGCGCGTGCGGGAAGTCCCCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACAATcc     <  1:479473/93‑1 (MQ=255)

                                                                                    cGAGGGGGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACAATcc     <  2:2750103/93‑1 (MQ=255)

                                                                                    cGAGGGGGCACTGGATTCCCTGCGCGTGCGGAAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACAATcc     <  2:1018935/93‑1 (MQ=255)

                                                                                      aGGGTGCACTGGATTCCCTGCGCGTGCGGAAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACAATCCTg   <  2:4459013/93‑1 (MQ=255)

                                                                                       ggggGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACAATCCTgg  >  1:4452271/1‑93 (MQ=255)

ACAGGTTGACGATAACCACCGTATCTACGCCGCGCGTTTTAACGAGCGCCTGCTCGCTGCCGTGCGGGTAACCTTAAGCGGCACCGAGGGAGCACTGGATTCCCTGCGCGTGCGGGAAGTCACCCGCCGTCGCGGTGTGGGGCAATATCTGCTGGAAGAGGTTTTGCGTAACAATCCTGG  >  NC_000913/3595707‑3595886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`