BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,596,295	A→G	V332A (GTG→GCG)	b3460 ←	LivJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,596,295	0	A	G	99.1%	679.3 / ‑3.1	213	V332A (GTG→GCG)	b3460	LivJ
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (100/111); total (102/111)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

Only 100 of 217 total aligned reads displayed.

GTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG  >  NC_000913/3596205‑3596384

gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         <  1:2484429/93‑1 (MQ=255)

gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         >  1:1956782/1‑93 (MQ=255)

  gccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGa                                                                                       <  2:3913949/93‑1 (MQ=255)

    cgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGt                                                                                     <  2:1740025/93‑1 (MQ=255)

    cgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGt                                                                                     <  2:1895337/93‑1 (MQ=255)

     gtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGtt                                                                                    <  1:10438/93‑1 (MQ=255)

     gtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGtt                                                                                    <  2:111269/93‑1 (MQ=255)

      ttGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTc                                                                                   >  1:3492423/1‑93 (MQ=255)

        ggCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGc                                                                                 <  2:1641180/93‑1 (MQ=255)

         gCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCt                                                                                >  2:2552583/1‑93 (MQ=255)

          cGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCtt                                                                               <  2:546622/93‑1 (MQ=255)

             gCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCa                                                                            >  1:3092502/1‑93 (MQ=255)

                aGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGt                                                                         >  2:1015362/1‑93 (MQ=255)

                aGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGt                                                                         <  1:2163168/93‑1 (MQ=255)

                aGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGCTCGCTTTCAGGt                                                                         >  1:4401833/1‑93 (MQ=255)

                    aaaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAttt                                                                     >  2:2455041/1‑93 (MQ=255)

                    aaaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAttt                                                                     >  2:3185519/1‑93 (MQ=255)

                     aaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTg                                                                    >  2:2783392/1‑93 (MQ=255)

                     aaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTg                                                                    >  1:265594/1‑93 (MQ=255)

                      aTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTgg                                                                   <  2:741122/93‑1 (MQ=255)

                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  2:3529943/93‑1 (MQ=255)

                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  2:2672045/93‑1 (MQ=255)

                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  1:2759073/93‑1 (MQ=255)

                        aCGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCg                                                                 >  2:2320064/1‑93 (MQ=255)

                          gCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAt                                                               <  1:2266248/93‑1 (MQ=255)

                           ccGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAtt                                                              <  2:794836/93‑1 (MQ=255)

                            cGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAttt                                                             <  1:3770895/93‑1 (MQ=255)

                             gAACTCAAAGCCTTTCAGATCGCCTTTCTCATCACAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTc                                                            <  1:3793047/93‑1 (MQ=255)

                              aaCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                                           <  1:1934169/93‑1 (MQ=255)

                              aaCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                                           <  2:3465218/93‑1 (MQ=255)

                               aCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGATCGCTTTCAGGTATTTGGCGATTTCAg                                                          >  1:435600/1‑93 (MQ=255)

                                 tCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCCTTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGcc                                                        >  2:2272694/1‑93 (MQ=255)

                                 ccAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGcc                                                        <  1:3138531/92‑1 (MQ=255)

                                  cAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                                       <  2:2100400/93‑1 (MQ=255)

                                  cAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                                       <  1:1497798/93‑1 (MQ=255)

                                  cAAAACCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                                       <  1:1591822/93‑1 (MQ=255)

                                   aaaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCgg                                                      >  1:923323/1‑93 (MQ=255)

                                   aaaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCgg                                                      <  1:2231896/93‑1 (MQ=255)

                                       ccTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCg                                                  <  2:1263625/93‑1 (MQ=255)

                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                >  2:3567468/1‑93 (MQ=255)

                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                >  1:2839401/1‑93 (MQ=255)

                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                <  1:127796/93‑1 (MQ=255)

                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCCTc                                                >  2:3012592/1‑93 (MQ=255)

                                            cAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGa                                             >  2:845594/1‑93 (MQ=255)

                                             aGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGAc                                            <  2:4054207/93‑1 (MQ=255)

                                              gATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACt                                           >  1:460521/1‑93 (MQ=255)

                                              gATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTAGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACt                                           >  1:734518/1‑93 (MQ=255)

                                              gATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACt                                           >  1:1570835/1‑93 (MQ=255)

                                               aTCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                                          <  2:3716413/93‑1 (MQ=255)

                                               aTCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                                          >  1:1734339/1‑93 (MQ=255)

                                                tCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                                         >  1:3302044/1‑93 (MQ=255)

                                                tCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                                         <  2:320366/93‑1 (MQ=255)

                                                 cGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCTGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                                        <  1:636158/93‑1 (MQ=255)

                                                 cGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                                        >  1:577397/1‑93 (MQ=255)

                                                 cGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                                        <  2:5936/93‑1 (MQ=255)

                                                  gCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                                       >  1:3669934/1‑93 (MQ=255)

                                                   ccTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTc                                      >  2:1899742/1‑93 (MQ=255)

                                                    cTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                                     <  1:2705150/93‑1 (MQ=255)

                                                      ttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAgg                                   <  1:2099416/93‑1 (MQ=255)

                                                      ttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAgg                                   >  2:3027281/1‑93 (MQ=255)

                                                       tctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGc                                  >  1:2273875/1‑93 (MQ=255)

                                                       tctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGc                                  <  2:1212547/93‑1 (MQ=255)

                                                       tctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGc                                  >  2:1980211/1‑93 (MQ=255)

                                                       tctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGc                                  <  1:922194/93‑1 (MQ=255)

                                                        ctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGcc                                 <  1:2987085/93‑1 (MQ=255)

                                                        ctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGcc                                 <  1:1710665/93‑1 (MQ=255)

                                                         tcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGGCTGATTCAGGccc                                <  2:4570200/93‑1 (MQ=255)

                                                          cATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCg                               >  1:3220511/1‑93 (MQ=255)

                                                           aTCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGc                              >  2:2753809/1‑93 (MQ=255)

                                                            tCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGCTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGACCGcc                             >  2:4325145/1‑93 (MQ=255)

                                                             cccAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCt                            <  1:4274674/93‑1 (MQ=255)

                                                             cccAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCt                            <  1:1166626/93‑1 (MQ=255)

                                                              ccAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTg                           <  1:2610656/93‑1 (MQ=255)

                                                               cAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGc                          <  2:3109550/93‑1 (MQ=255)

                                                                 ggTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaa                        >  2:704454/1‑93 (MQ=255)

                                                                 ggTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaa                        >  2:3815179/1‑93 (MQ=255)

                                                                  gTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaaa                       <  2:892032/93‑1 (MQ=255)

                                                                    cAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa                     <  1:2304619/93‑1 (MQ=255)

                                                                     aGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAt                    >  1:2086725/1‑93 (MQ=255)

                                                                      gCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAtt                   <  1:1828403/93‑1 (MQ=255)

                                                                      gCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAtt                   <  1:3426837/93‑1 (MQ=255)

                                                                       cGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTg                  <  2:3490288/93‑1 (MQ=255)

                                                                        ggTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGc                 >  1:3346652/1‑93 (MQ=255)

                                                                         gTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa                <  1:2104128/93‑1 (MQ=255)

                                                                            ccATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcg             >  1:550949/1‑93 (MQ=255)

                                                                             cATTGCGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcgc            <  2:1879047/93‑1 (MQ=255)

                                                                             cATTACGGTATCCGCGGAGTTCGCTTTCGGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcgc            <  1:1233586/93‑1 (MQ=255)

                                                                             cATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcgc            <  1:1366453/93‑1 (MQ=255)

                                                                             cATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcgc            >  1:497019/1‑93 (MQ=255)

                                                                             cATTACGGTATCCGCGGAGTTCGCTTTCAGGGATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCCAAGATTGCAgcgc            >  2:2505856/1‑93 (MQ=255)

                                                                              aTTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGcgcg           >  2:1501826/1‑93 (MQ=255)

                                                                               ttACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCgcgg          >  1:4586424/1‑93 (MQ=255)

                                                                               ttACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCgcgg          >  1:4594530/1‑93 (MQ=255)

                                                                                 aCGGTATCCGCGGAGTTCGCTGTCAGGTATTTGGCGATTTCAGCGGGATCGTCAGACTGATTGAGGCCCGCTTGCAAAGATTGCAGCgcggcg        <  1:1161391/93‑1 (MQ=255)

                                                                                  cGGTCTCCGCGGATTTCGCTTTCAGGTATTTGGCTATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGt       <  1:4037623/93‑1 (MQ=255)

                                                                                   ggTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTa      <  1:2945016/93‑1 (MQ=255)

                                                                                      aTCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggt   <  2:1401125/93‑1 (MQ=255)

                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  >  1:4624332/1‑93 (MQ=255)

                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  <  1:895767/93‑1 (MQ=255)

                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  >  1:981903/1‑93 (MQ=255)

GTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG  >  NC_000913/3596205‑3596384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`