BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,604,952	T→C	S291P (TCC→CCC)	zntA →	zinc‑transporting ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,604,952	0	T	C	98.5%	405.0 / ‑3.2	131	S291P (TCC→CCC)	zntA	zinc‑transporting ATPase
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (56/72); total (57/74)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 138 total aligned reads displayed.

ATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGA  >  NC_000913/3604862‑3605040

aTTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAAccc                                                                                        >  2:649687/1‑93 (MQ=255)

 ttGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCa                                                                                       <  1:654804/93‑1 (MQ=255)

 ttGAAAGCGGCGCAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTACCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCa                                                                                       <  1:2504670/93‑1 (MQ=255)

   gAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCAtt                                                                                     >  2:2563078/1‑93 (MQ=255)

    aaGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTc                                                                                    <  1:2151883/93‑1 (MQ=255)

    aaGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGGGAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACACATTc                                                                                    >  2:3183710/1‑93 (MQ=255)

     aGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTcc                                                                                   >  2:4037093/1‑93 (MQ=255)

       tCGCCGCAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCgg                                                                                 <  1:62210/93‑1 (MQ=255)

        cgccgcAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGt                                                                                <  2:738198/93‑1 (MQ=255)

        cgccgcAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGt                                                                                <  2:2377933/93‑1 (MQ=255)

        cgccgcAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGt                                                                                <  2:1304993/93‑1 (MQ=255)

         gccgcAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTg                                                                               <  2:1997523/93‑1 (MQ=255)

         cccgcAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTg                                                                               <  2:2508806/92‑1 (MQ=255)

           ngcAGGTGGGCGTTTGCCTGCNGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGa                                                                             <  1:3773437/92‑1 (MQ=255)

           cgcAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGa                                                                             >  1:3733449/1‑93 (MQ=255)

              agatGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAgcg                                                                          <  1:639882/90‑1 (MQ=255)

               ggTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAgcgc                                                                         <  1:618211/93‑1 (MQ=255)

                  gggCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCa                                                                      >  2:3542340/1‑93 (MQ=255)

                    gCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGCCCGGCGAATCCATTCCGGTGGAGCGCGCAAc                                                                    <  2:4035921/93‑1 (MQ=255)

                      gTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACgg                                                                  <  2:154998/93‑1 (MQ=255)

                      gTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACgg                                                                  <  2:154996/93‑1 (MQ=255)

                      gTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACgg                                                                  <  1:2187115/93‑1 (MQ=255)

                       tttGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACggg                                                                 >  2:338118/1‑93 (MQ=255)

                         tgcctgccGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCg                                                               >  1:1512544/1‑93 (MQ=255)

                         tgcctgccGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCg                                                               <  1:509222/93‑1 (MQ=255)

                          gcctgccGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGa                                                              <  1:2061411/93‑1 (MQ=255)

                           cctgccGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGGAAGCGCCCTGACCGGCGAACACATTCCGGTGGAGCGCGCAACGGGCGAt                                                             >  2:415034/1‑93 (MQ=255)

                            ctgccGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATa                                                            <  1:821434/93‑1 (MQ=255)

                             tgccGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATaa                                                           >  2:1866651/1‑93 (MQ=255)

                              gccGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCCTTCCGGTGGAGCGCGCAACGGGCGATaaa                                                          >  1:3721701/1‑93 (MQ=255)

                                 gACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTc                                                       <  2:4556759/93‑1 (MQ=255)

                                 gACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTc                                                       <  2:1281473/93‑1 (MQ=255)

                                  aCGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTcc                                                      <  1:4072142/93‑1 (MQ=255)

                                   cGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTccc                                                     <  2:4461950/93‑1 (MQ=255)

                                   cGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTccc                                                     <  1:4376197/93‑1 (MQ=255)

                                       aaaCTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCctgct                                                 <  1:1326564/93‑1 (MQ=255)

                                         aCTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTgg                                               <  2:3924356/93‑1 (MQ=255)

                                         aCTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTgg                                               >  1:4030594/1‑93 (MQ=255)

                                          cTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGt                                              >  2:4717778/1‑93 (MQ=255)

                                          cTGCTCTCACCGTTTGCCAGTTTAGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGt                                              <  2:3393854/93‑1 (MQ=255)

                                           tGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATCAAGTCCCTGCTGGTg                                             >  1:2578189/1‑93 (MQ=255)

                                              tctcACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccc                                          >  1:1013983/1‑92 (MQ=255)

                                              tctcACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGcca                                          >  1:1200140/1‑93 (MQ=255)

                                               ctcACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGTGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccac                                         <  2:3943454/93‑1 (MQ=255)

                                               ctcACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccac                                         >  1:3064562/1‑93 (MQ=255)

                                                tcACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccacc                                        >  2:3628100/1‑93 (MQ=255)

                                                tcACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccacc                                        >  1:4582172/1‑93 (MQ=255)

                                                tcACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccacc                                        <  2:1563445/93‑1 (MQ=255)

                                                 cACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccacca                                       >  2:2733377/1‑93 (MQ=255)

                                                 cACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccacca                                       <  2:716472/93‑1 (MQ=255)

                                                 cACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccacca                                       <  1:1590293/93‑1 (MQ=255)

                                                 cACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccacca                                       >  2:2341221/1‑93 (MQ=255)

                                                 cACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGccacca                                       <  2:1678084/93‑1 (MQ=255)

                                                  aCCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAg                                      <  1:2521118/93‑1 (MQ=255)

                                                  aCCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAg                                      <  1:2521117/93‑1 (MQ=255)

                                                   ccGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGc                                     <  2:3227065/93‑1 (MQ=255)

                                                   ccGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGc                                     <  1:35407/93‑1 (MQ=255)

                                                   ccGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCAAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGc                                     <  1:35409/93‑1 (MQ=255)

                                                     gTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGt                                   <  1:528270/93‑1 (MQ=255)

                                                      tttGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATACAGTCCCTGCTGGTGCCACCAGCGTa                                  >  2:2089362/1‑93 (MQ=255)

                                                      tttGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTa                                  >  1:2353150/1‑93 (MQ=255)

                                                      tttGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTa                                  >  2:4470254/1‑93 (MQ=255)

                                                       ttGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAg                                 <  2:1479507/93‑1 (MQ=255)

                                                          ccAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGAcc                              >  1:1310576/1‑93 (MQ=255)

                                                           cAGTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCg                             >  2:1003126/1‑93 (MQ=255)

                                                             gTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTc                           >  1:2652977/1‑93 (MQ=255)

                                                             gTTTTGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTc                           <  2:1144099/93‑1 (MQ=255)

                                                              ttttGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCt                          >  1:2382171/1‑93 (MQ=255)

                                                              ttttGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCt                          <  1:4145503/93‑1 (MQ=255)

                                                               tttGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTg                         <  2:1994623/93‑1 (MQ=255)

                                                               tttGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTg                         <  1:1688655/93‑1 (MQ=255)

                                                                ttGATGAAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTgg                        <  1:3986134/93‑1 (MQ=255)

                                                                  gatgaAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTg                      >  2:301902/1‑93 (MQ=255)

                                                                  gatgaAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTg                      >  1:1149046/1‑93 (MQ=255)

                                                                   atgaAAGCGCCCTGACCGTCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAGGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGa                     <  2:3420785/93‑1 (MQ=255)

                                                                     gaAAGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGCCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACg                   >  1:2751556/1‑93 (MQ=255)

                                                                      aaaGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGt                  <  1:4313126/93‑1 (MQ=255)

                                                                       aaGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGtt                 <  1:1612381/93‑1 (MQ=255)

                                                                       aaGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGtt                 >  1:4538022/1‑93 (MQ=255)

                                                                       aaGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGtt                 <  1:4350494/93‑1 (MQ=255)

                                                                       aaGCGCCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGtt                 <  1:2227763/93‑1 (MQ=255)

                                                                       aaGCGCCCGGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGtt                 <  2:361906/93‑1 (MQ=255)

                                                                          cgcCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGa              <  2:1478339/93‑1 (MQ=255)

                                                                           gcCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGaa             >  2:1400711/1‑93 (MQ=255)

                                                                           gcCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGaa             <  1:4393933/93‑1 (MQ=255)

                                                                           gcCCTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGaa             >  1:3121608/1‑93 (MQ=255)

                                                                            cccTGACCGGCGAAGCCATTCCGGTCGAGCGCGCAATTGATAATAAAGTCCCTGCTGGTGCAACCACCGTAGACCGTCTGGTGACGTTGGAAg            <  1:2493303/93‑1 (MQ=255)

                                                                            cccTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAg            >  2:4405033/1‑93 (MQ=255)

                                                                            cccTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAg            <  1:2493301/93‑1 (MQ=255)

                                                                             ccTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAgg           >  1:3576200/1‑92 (MQ=255)

                                                                             ccTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGt           >  1:2416723/1‑93 (MQ=255)

                                                                             ccTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCGGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGt           >  1:1505173/1‑93 (MQ=255)

                                                                              cTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTg          <  2:3090052/93‑1 (MQ=255)

                                                                              cTGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTg          >  1:275237/1‑93 (MQ=255)

                                                                               tGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGc         <  2:1163385/93‑1 (MQ=255)

                                                                               tGACCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGc         <  2:4583592/93‑1 (MQ=255)

                                                                                 aCCGGCGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACACTCTGGTGACGTTGGAAGTGCTg       >  1:489619/1‑93 (MQ=255)

                                                                                   tggCGAAGCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGGGACGGTGGAAGTGCTggc     >  2:2881563/2‑91 (MQ=255)

                                                                                      cGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGa  >  2:543851/1‑93 (MQ=255)

                                                                                      cGAACCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGa  <  1:2730869/93‑1 (MQ=255)

ATTGAAGTCGCCGCAGGTGGGCGTTTGCCTGCCGACGGTAAACTGCTCTCACCGTTTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGA  >  NC_000913/3604862‑3605040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`